Amino acid dipepetide frequency for [Clostridium] polysaccharolyticum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.413AlaAla: 5.413 ± 0.111	1.045AlaCys: 1.045 ± 0.037	3.869AlaAsp: 3.869 ± 0.078	4.174AlaGlu: 4.174 ± 0.078	3.016AlaPhe: 3.016 ± 0.059	5.267AlaGly: 5.267 ± 0.086	0.959AlaHis: 0.959 ± 0.034	5.06AlaIle: 5.06 ± 0.086	5.33AlaLys: 5.33 ± 0.087	5.76AlaLeu: 5.76 ± 0.078	1.981AlaMet: 1.981 ± 0.047	2.909AlaAsn: 2.909 ± 0.065	1.867AlaPro: 1.867 ± 0.058	2.059AlaGln: 2.059 ± 0.056	2.313AlaArg: 2.313 ± 0.05	4.086AlaSer: 4.086 ± 0.066	2.999AlaThr: 2.999 ± 0.064	5.328AlaVal: 5.328 ± 0.074	0.544AlaTrp: 0.544 ± 0.027	2.796AlaTyr: 2.796 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	0.866CysAla: 0.866 ± 0.03	0.276CysCys: 0.276 ± 0.019	0.849CysAsp: 0.849 ± 0.033	0.875CysGlu: 0.875 ± 0.031	0.729CysPhe: 0.729 ± 0.029	1.256CysGly: 1.256 ± 0.034	0.264CysHis: 0.264 ± 0.017	1.27CysIle: 1.27 ± 0.041	1.083CysLys: 1.083 ± 0.032	1.223CysLeu: 1.223 ± 0.035	0.47CysMet: 0.47 ± 0.021	0.772CysAsn: 0.772 ± 0.028	0.518CysPro: 0.518 ± 0.023	0.439CysGln: 0.439 ± 0.02	0.509CysArg: 0.509 ± 0.022	0.988CysSer: 0.988 ± 0.027	0.756CysThr: 0.756 ± 0.028	1.0CysVal: 1.0 ± 0.037	0.113CysTrp: 0.113 ± 0.011	0.682CysTyr: 0.682 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	3.481AspAla: 3.481 ± 0.069	0.807AspCys: 0.807 ± 0.031	2.423AspAsp: 2.423 ± 0.056	4.122AspGlu: 4.122 ± 0.075	2.639AspPhe: 2.639 ± 0.047	4.027AspGly: 4.027 ± 0.082	0.739AspHis: 0.739 ± 0.035	4.76AspIle: 4.76 ± 0.08	4.259AspLys: 4.259 ± 0.074	4.468AspLeu: 4.468 ± 0.062	1.65AspMet: 1.65 ± 0.041	2.488AspAsn: 2.488 ± 0.054	1.446AspPro: 1.446 ± 0.039	1.282AspGln: 1.282 ± 0.037	1.875AspArg: 1.875 ± 0.042	3.378AspSer: 3.378 ± 0.067	2.766AspThr: 2.766 ± 0.052	3.858AspVal: 3.858 ± 0.061	0.607AspTrp: 0.607 ± 0.027	2.895AspTyr: 2.895 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	5.067GluAla: 5.067 ± 0.079	0.904GluCys: 0.904 ± 0.03	4.106GluAsp: 4.106 ± 0.072	7.557GluGlu: 7.557 ± 0.125	2.923GluPhe: 2.923 ± 0.061	4.094GluGly: 4.094 ± 0.069	1.435GluHis: 1.435 ± 0.033	5.855GluIle: 5.855 ± 0.098	7.433GluLys: 7.433 ± 0.107	6.912GluLeu: 6.912 ± 0.094	2.271GluMet: 2.271 ± 0.049	4.431GluAsn: 4.431 ± 0.073	1.914GluPro: 1.914 ± 0.055	3.297GluGln: 3.297 ± 0.07	3.05GluArg: 3.05 ± 0.058	3.609GluSer: 3.609 ± 0.069	3.712GluThr: 3.712 ± 0.069	4.61GluVal: 4.61 ± 0.077	0.613GluTrp: 0.613 ± 0.027	3.328GluTyr: 3.328 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	2.84PheAla: 2.84 ± 0.052	0.787PheCys: 0.787 ± 0.03	2.556PheAsp: 2.556 ± 0.06	2.855PheGlu: 2.855 ± 0.056	1.897PhePhe: 1.897 ± 0.048	2.949PheGly: 2.949 ± 0.057	0.943PheHis: 0.943 ± 0.032	3.245PheIle: 3.245 ± 0.072	2.315PheLys: 2.315 ± 0.047	3.944PheLeu: 3.944 ± 0.076	1.18PheMet: 1.18 ± 0.036	1.756PheAsn: 1.756 ± 0.043	1.259PhePro: 1.259 ± 0.032	1.633PheGln: 1.633 ± 0.044	1.571PheArg: 1.571 ± 0.04	2.897PheSer: 2.897 ± 0.051	2.421PheThr: 2.421 ± 0.056	2.985PheVal: 2.985 ± 0.055	0.417PheTrp: 0.417 ± 0.026	1.967PheTyr: 1.967 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	4.215GlyAla: 4.215 ± 0.079	1.181GlyCys: 1.181 ± 0.042	3.25GlyAsp: 3.25 ± 0.069	4.282GlyGlu: 4.282 ± 0.074	3.05GlyPhe: 3.05 ± 0.057	4.256GlyGly: 4.256 ± 0.075	1.039GlyHis: 1.039 ± 0.033	6.006GlyIle: 6.006 ± 0.084	6.063GlyLys: 6.063 ± 0.082	5.182GlyLeu: 5.182 ± 0.076	2.123GlyMet: 2.123 ± 0.052	3.395GlyAsn: 3.395 ± 0.076	1.118GlyPro: 1.118 ± 0.037	2.024GlyGln: 2.024 ± 0.049	2.522GlyArg: 2.522 ± 0.048	3.901GlySer: 3.901 ± 0.079	4.178GlyThr: 4.178 ± 0.075	4.667GlyVal: 4.667 ± 0.067	0.63GlyTrp: 0.63 ± 0.03	3.235GlyTyr: 3.235 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	1.013HisAla: 1.013 ± 0.032	0.283HisCys: 0.283 ± 0.015	0.884HisAsp: 0.884 ± 0.031	0.967HisGlu: 0.967 ± 0.028	0.86HisPhe: 0.86 ± 0.029	1.135HisGly: 1.135 ± 0.04	0.389HisHis: 0.389 ± 0.025	1.415HisIle: 1.415 ± 0.039	1.154HisLys: 1.154 ± 0.039	1.362HisLeu: 1.362 ± 0.037	0.515HisMet: 0.515 ± 0.023	0.907HisAsn: 0.907 ± 0.031	0.716HisPro: 0.716 ± 0.026	0.49HisGln: 0.49 ± 0.021	0.598HisArg: 0.598 ± 0.025	1.043HisSer: 1.043 ± 0.031	0.843HisThr: 0.843 ± 0.027	1.147HisVal: 1.147 ± 0.034	0.158HisTrp: 0.158 ± 0.014	0.764HisTyr: 0.764 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.69IleAla: 5.69 ± 0.094	1.374IleCys: 1.374 ± 0.037	4.135IleAsp: 4.135 ± 0.071	5.72IleGlu: 5.72 ± 0.087	2.837IlePhe: 2.837 ± 0.058	5.19IleGly: 5.19 ± 0.083	1.472IleHis: 1.472 ± 0.041	5.674IleIle: 5.674 ± 0.099	5.399IleLys: 5.399 ± 0.086	7.072IleLeu: 7.072 ± 0.105	2.092IleMet: 2.092 ± 0.043	3.488IleAsn: 3.488 ± 0.064	2.891IlePro: 2.891 ± 0.053	2.926IleGln: 2.926 ± 0.058	3.262IleArg: 3.262 ± 0.059	5.213IleSer: 5.213 ± 0.074	4.505IleThr: 4.505 ± 0.068	5.293IleVal: 5.293 ± 0.081	0.638IleTrp: 0.638 ± 0.026	3.002IleTyr: 3.002 ± 0.056	0.0IleXaa: 0.0 ± 0.0
Lys	5.345LysAla: 5.345 ± 0.084	0.831LysCys: 0.831 ± 0.027	4.541LysAsp: 4.541 ± 0.065	8.808LysGlu: 8.808 ± 0.14	2.374LysPhe: 2.374 ± 0.052	4.626LysGly: 4.626 ± 0.065	1.141LysHis: 1.141 ± 0.031	5.681LysIle: 5.681 ± 0.082	7.689LysLys: 7.689 ± 0.108	6.285LysLeu: 6.285 ± 0.08	2.434LysMet: 2.434 ± 0.041	4.407LysAsn: 4.407 ± 0.069	2.198LysPro: 2.198 ± 0.05	3.063LysGln: 3.063 ± 0.06	3.382LysArg: 3.382 ± 0.06	4.021LysSer: 4.021 ± 0.065	4.173LysThr: 4.173 ± 0.074	5.163LysVal: 5.163 ± 0.077	0.694LysTrp: 0.694 ± 0.027	3.34LysTyr: 3.34 ± 0.058	0.0LysXaa: 0.0 ± 0.0
Leu	5.989LeuAla: 5.989 ± 0.088	1.382LeuCys: 1.382 ± 0.036	4.908LeuAsp: 4.908 ± 0.07	6.873LeuGlu: 6.873 ± 0.1	3.775LeuPhe: 3.775 ± 0.067	5.602LeuGly: 5.602 ± 0.082	1.538LeuHis: 1.538 ± 0.048	6.045LeuIle: 6.045 ± 0.101	6.676LeuLys: 6.676 ± 0.088	7.871LeuLeu: 7.871 ± 0.121	2.237LeuMet: 2.237 ± 0.041	4.349LeuAsn: 4.349 ± 0.074	2.889LeuPro: 2.889 ± 0.062	2.794LeuGln: 2.794 ± 0.054	3.207LeuArg: 3.207 ± 0.063	6.279LeuSer: 6.279 ± 0.082	4.493LeuThr: 4.493 ± 0.072	5.465LeuVal: 5.465 ± 0.074	0.694LeuTrp: 0.694 ± 0.026	3.448LeuTyr: 3.448 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	2.008MetAla: 2.008 ± 0.049	0.375MetCys: 0.375 ± 0.019	1.771MetAsp: 1.771 ± 0.036	2.621MetGlu: 2.621 ± 0.051	1.086MetPhe: 1.086 ± 0.036	1.738MetGly: 1.738 ± 0.044	0.426MetHis: 0.426 ± 0.021	2.047MetIle: 2.047 ± 0.045	2.834MetLys: 2.834 ± 0.051	2.622MetLeu: 2.622 ± 0.063	0.802MetMet: 0.802 ± 0.028	1.641MetAsn: 1.641 ± 0.038	1.01MetPro: 1.01 ± 0.03	1.009MetGln: 1.009 ± 0.031	1.069MetArg: 1.069 ± 0.035	1.695MetSer: 1.695 ± 0.034	1.354MetThr: 1.354 ± 0.034	1.777MetVal: 1.777 ± 0.043	0.197MetTrp: 0.197 ± 0.014	1.002MetTyr: 1.002 ± 0.031	0.0MetXaa: 0.0 ± 0.0
Asn	3.262AsnAla: 3.262 ± 0.059	0.766AsnCys: 0.766 ± 0.029	2.296AsnAsp: 2.296 ± 0.047	3.458AsnGlu: 3.458 ± 0.064	1.86AsnPhe: 1.86 ± 0.049	4.061AsnGly: 4.061 ± 0.077	0.871AsnHis: 0.871 ± 0.03	4.033AsnIle: 4.033 ± 0.056	3.413AsnLys: 3.413 ± 0.064	4.089AsnLeu: 4.089 ± 0.07	1.558AsnMet: 1.558 ± 0.041	2.339AsnAsn: 2.339 ± 0.06	1.962AsnPro: 1.962 ± 0.043	2.124AsnGln: 2.124 ± 0.053	2.122AsnArg: 2.122 ± 0.048	2.877AsnSer: 2.877 ± 0.061	2.679AsnThr: 2.679 ± 0.059	3.458AsnVal: 3.458 ± 0.068	0.514AsnTrp: 0.514 ± 0.024	2.182AsnTyr: 2.182 ± 0.049	0.0AsnXaa: 0.0 ± 0.0
Pro	2.118ProAla: 2.118 ± 0.057	0.423ProCys: 0.423 ± 0.021	2.051ProAsp: 2.051 ± 0.048	2.63ProGlu: 2.63 ± 0.052	1.445ProPhe: 1.445 ± 0.038	1.741ProGly: 1.741 ± 0.043	0.451ProHis: 0.451 ± 0.022	2.009ProIle: 2.009 ± 0.047	1.974ProLys: 1.974 ± 0.044	2.377ProLeu: 2.377 ± 0.046	0.801ProMet: 0.801 ± 0.027	1.263ProAsn: 1.263 ± 0.036	0.56ProPro: 0.56 ± 0.028	0.805ProGln: 0.805 ± 0.028	0.845ProArg: 0.845 ± 0.03	2.239ProSer: 2.239 ± 0.079	1.192ProThr: 1.192 ± 0.04	2.828ProVal: 2.828 ± 0.067	0.268ProTrp: 0.268 ± 0.015	1.462ProTyr: 1.462 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	2.244GlnAla: 2.244 ± 0.046	0.418GlnCys: 0.418 ± 0.02	1.646GlnAsp: 1.646 ± 0.042	2.865GlnGlu: 2.865 ± 0.063	1.474GlnPhe: 1.474 ± 0.04	1.918GlnGly: 1.918 ± 0.046	0.488GlnHis: 0.488 ± 0.018	2.835GlnIle: 2.835 ± 0.053	3.201GlnLys: 3.201 ± 0.056	2.984GlnLeu: 2.984 ± 0.055	1.168GlnMet: 1.168 ± 0.036	1.949GlnAsn: 1.949 ± 0.041	0.843GlnPro: 0.843 ± 0.028	1.252GlnGln: 1.252 ± 0.044	1.246GlnArg: 1.246 ± 0.034	1.823GlnSer: 1.823 ± 0.042	1.667GlnThr: 1.667 ± 0.045	2.376GlnVal: 2.376 ± 0.052	0.338GlnTrp: 0.338 ± 0.021	1.638GlnTyr: 1.638 ± 0.044	0.0GlnXaa: 0.0 ± 0.0
Arg	2.047ArgAla: 2.047 ± 0.045	0.472ArgCys: 0.472 ± 0.023	1.811ArgAsp: 1.811 ± 0.043	3.103ArgGlu: 3.103 ± 0.068	1.694ArgPhe: 1.694 ± 0.041	2.083ArgGly: 2.083 ± 0.045	0.619ArgHis: 0.619 ± 0.022	3.426ArgIle: 3.426 ± 0.063	3.652ArgLys: 3.652 ± 0.066	3.142ArgLeu: 3.142 ± 0.063	1.27ArgMet: 1.27 ± 0.032	2.248ArgAsn: 2.248 ± 0.044	1.006ArgPro: 1.006 ± 0.037	1.384ArgGln: 1.384 ± 0.037	1.622ArgArg: 1.622 ± 0.044	1.897ArgSer: 1.897 ± 0.042	2.025ArgThr: 2.025 ± 0.047	2.312ArgVal: 2.312 ± 0.045	0.31ArgTrp: 0.31 ± 0.017	1.753ArgTyr: 1.753 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	3.854SerAla: 3.854 ± 0.072	0.914SerCys: 0.914 ± 0.031	3.261SerAsp: 3.261 ± 0.066	3.997SerGlu: 3.997 ± 0.064	2.916SerPhe: 2.916 ± 0.053	4.679SerGly: 4.679 ± 0.082	0.952SerHis: 0.952 ± 0.031	5.021SerIle: 5.021 ± 0.079	4.53SerLys: 4.53 ± 0.064	5.391SerLeu: 5.391 ± 0.079	1.816SerMet: 1.816 ± 0.046	2.997SerAsn: 2.997 ± 0.066	1.555SerPro: 1.555 ± 0.039	2.108SerGln: 2.108 ± 0.044	2.336SerArg: 2.336 ± 0.051	4.294SerSer: 4.294 ± 0.102	3.042SerThr: 3.042 ± 0.059	4.495SerVal: 4.495 ± 0.085	0.581SerTrp: 0.581 ± 0.026	2.902SerTyr: 2.902 ± 0.063	0.0SerXaa: 0.0 ± 0.0
Thr	3.747ThrAla: 3.747 ± 0.067	0.706ThrCys: 0.706 ± 0.025	2.938ThrAsp: 2.938 ± 0.068	3.478ThrGlu: 3.478 ± 0.063	2.278ThrPhe: 2.278 ± 0.051	4.357ThrGly: 4.357 ± 0.072	0.77ThrHis: 0.77 ± 0.029	4.272ThrIle: 4.272 ± 0.073	3.756ThrLys: 3.756 ± 0.061	4.5ThrLeu: 4.5 ± 0.072	1.377ThrMet: 1.377 ± 0.036	2.248ThrAsn: 2.248 ± 0.048	1.759ThrPro: 1.759 ± 0.05	1.448ThrGln: 1.448 ± 0.039	1.753ThrArg: 1.753 ± 0.038	3.43ThrSer: 3.43 ± 0.07	2.61ThrThr: 2.61 ± 0.07	4.219ThrVal: 4.219 ± 0.078	0.473ThrTrp: 0.473 ± 0.021	2.376ThrTyr: 2.376 ± 0.064	0.0ThrXaa: 0.0 ± 0.0
Val	4.459ValAla: 4.459 ± 0.074	1.114ValCys: 1.114 ± 0.03	3.522ValAsp: 3.522 ± 0.06	4.843ValGlu: 4.843 ± 0.071	3.132ValPhe: 3.132 ± 0.064	3.658ValGly: 3.658 ± 0.075	1.07ValHis: 1.07 ± 0.033	5.353ValIle: 5.353 ± 0.084	5.602ValLys: 5.602 ± 0.085	6.684ValLeu: 6.684 ± 0.097	1.96ValMet: 1.96 ± 0.042	3.496ValAsn: 3.496 ± 0.062	2.373ValPro: 2.373 ± 0.051	2.096ValGln: 2.096 ± 0.044	2.499ValArg: 2.499 ± 0.054	4.864ValSer: 4.864 ± 0.069	4.235ValThr: 4.235 ± 0.086	4.747ValVal: 4.747 ± 0.081	0.55ValTrp: 0.55 ± 0.025	2.919ValTyr: 2.919 ± 0.063	0.001ValXaa: 0.001 ± 0.001
Trp	0.489TrpAla: 0.489 ± 0.021	0.161TrpCys: 0.161 ± 0.013	0.516TrpAsp: 0.516 ± 0.024	0.613TrpGlu: 0.613 ± 0.024	0.414TrpPhe: 0.414 ± 0.02	0.635TrpGly: 0.635 ± 0.03	0.178TrpHis: 0.178 ± 0.013	0.657TrpIle: 0.657 ± 0.026	0.811TrpLys: 0.811 ± 0.026	0.705TrpLeu: 0.705 ± 0.027	0.263TrpMet: 0.263 ± 0.017	0.638TrpAsn: 0.638 ± 0.025	0.201TrpPro: 0.201 ± 0.016	0.28TrpGln: 0.28 ± 0.017	0.315TrpArg: 0.315 ± 0.018	0.522TrpSer: 0.522 ± 0.026	0.427TrpThr: 0.427 ± 0.022	0.463TrpVal: 0.463 ± 0.023	0.113TrpTrp: 0.113 ± 0.011	0.427TrpTyr: 0.427 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.627TyrAla: 2.627 ± 0.059	0.719TyrCys: 0.719 ± 0.031	2.639TyrAsp: 2.639 ± 0.066	3.279TyrGlu: 3.279 ± 0.064	2.015TyrPhe: 2.015 ± 0.054	3.043TyrGly: 3.043 ± 0.061	0.901TyrHis: 0.901 ± 0.026	3.211TyrIle: 3.211 ± 0.055	3.023TyrLys: 3.023 ± 0.05	3.94TyrLeu: 3.94 ± 0.077	1.112TyrMet: 1.112 ± 0.031	2.204TyrAsn: 2.204 ± 0.055	1.397TyrPro: 1.397 ± 0.043	1.862TyrGln: 1.862 ± 0.051	1.779TyrArg: 1.779 ± 0.044	2.587TyrSer: 2.587 ± 0.051	2.415TyrThr: 2.415 ± 0.053	2.989TyrVal: 2.989 ± 0.058	0.376TyrTrp: 0.376 ± 0.02	2.23TyrTyr: 2.23 ± 0.06	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 3018 proteins (1022225 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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