Pelargonium vein banding virus
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C9DT06|C9DT06_9VIRU Uncharacterized protein OS=Pelargonium vein banding virus OX=671126 PE=4 SV=1
MM1 pKa = 7.75 SEE3 pKa = 4.29 RR4 pKa = 11.84 FEE6 pKa = 3.93 QAIQKK11 pKa = 8.73 WYY13 pKa = 10.45 NEE15 pKa = 3.75 SRR17 pKa = 11.84 TADD20 pKa = 3.75 LRR22 pKa = 11.84 YY23 pKa = 10.0 LDD25 pKa = 5.15 LATEE29 pKa = 4.36 SPTVKK34 pKa = 10.31 DD35 pKa = 3.58 YY36 pKa = 11.79 CSVINNNVSVIYY48 pKa = 10.4 DD49 pKa = 3.53 RR50 pKa = 11.84 LLLHH54 pKa = 6.55 SKK56 pKa = 10.18 VAIKK60 pKa = 9.28 DD61 pKa = 3.37 TYY63 pKa = 10.83 KK64 pKa = 10.53 ILEE67 pKa = 4.32 AQEE70 pKa = 4.18 ANQDD74 pKa = 3.3 ILVQEE79 pKa = 4.21 IRR81 pKa = 11.84 RR82 pKa = 11.84 LSKK85 pKa = 10.2 RR86 pKa = 11.84 VKK88 pKa = 10.27 ALEE91 pKa = 4.15 AEE93 pKa = 4.25 ALASKK98 pKa = 10.18 PLTAEE103 pKa = 3.89 QVRR106 pKa = 11.84 EE107 pKa = 4.03 LVKK110 pKa = 10.82 EE111 pKa = 4.06 IAAQPKK117 pKa = 9.83 LVEE120 pKa = 4.04 EE121 pKa = 4.28 QAFKK125 pKa = 10.2 LTTDD129 pKa = 3.54 LRR131 pKa = 11.84 SQVLQVKK138 pKa = 9.31 EE139 pKa = 3.96 LVEE142 pKa = 4.33 KK143 pKa = 10.32 VQHH146 pKa = 6.26 LLVSS150 pKa = 4.15
Molecular weight: 17.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.923
IPC2_protein 5.97
IPC_protein 6.008
Toseland 6.287
ProMoST 6.198
Dawson 6.198
Bjellqvist 6.173
Wikipedia 6.173
Rodwell 6.173
Grimsley 6.402
Solomon 6.198
Lehninger 6.186
Nozaki 6.402
DTASelect 6.576
Thurlkill 6.576
EMBOSS 6.547
Sillero 6.532
Patrickios 4.584
IPC_peptide 6.211
IPC2_peptide 6.402
IPC2.peptide.svr19 6.582
Protein with the highest isoelectric point:
>tr|C9DT07|C9DT07_9VIRU Reverse transcriptase OS=Pelargonium vein banding virus OX=671126 PE=4 SV=1
MM1 pKa = 7.46 SRR3 pKa = 11.84 PDD5 pKa = 3.23 SATTAEE11 pKa = 4.57 YY12 pKa = 10.16 IAAAQATEE20 pKa = 4.11 ALGSPAEE27 pKa = 4.41 GFLSVKK33 pKa = 10.23 NIPPGSSSQTALLIKK48 pKa = 10.35 QNNFLLEE55 pKa = 4.18 LVLDD59 pKa = 3.85 LHH61 pKa = 6.9 RR62 pKa = 11.84 KK63 pKa = 8.29 INGVEE68 pKa = 3.85 ARR70 pKa = 11.84 LSRR73 pKa = 11.84 AKK75 pKa = 10.43 EE76 pKa = 3.74 PAEE79 pKa = 4.24 GSQGQGLEE87 pKa = 4.22 EE88 pKa = 5.03 IIQKK92 pKa = 10.1 LDD94 pKa = 3.13 KK95 pKa = 11.16 LKK97 pKa = 10.83 IGDD100 pKa = 4.3 GEE102 pKa = 4.42 QKK104 pKa = 8.24 THH106 pKa = 6.6 SIKK109 pKa = 11.0 GPGKK113 pKa = 9.13 VYY115 pKa = 9.99 FYY117 pKa = 10.82 RR118 pKa = 11.84 DD119 pKa = 3.51 PKK121 pKa = 11.05 ALAEE125 pKa = 4.05 EE126 pKa = 4.37 EE127 pKa = 3.95 RR128 pKa = 11.84 RR129 pKa = 11.84 KK130 pKa = 10.73 AKK132 pKa = 10.12 LRR134 pKa = 11.84 YY135 pKa = 9.09 AA136 pKa = 3.92
Molecular weight: 14.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.632
IPC2_protein 8.478
IPC_protein 8.492
Toseland 9.297
ProMoST 8.946
Dawson 9.516
Bjellqvist 9.121
Wikipedia 9.648
Rodwell 9.867
Grimsley 9.575
Solomon 9.619
Lehninger 9.589
Nozaki 9.224
DTASelect 9.136
Thurlkill 9.341
EMBOSS 9.692
Sillero 9.414
Patrickios 5.461
IPC_peptide 9.604
IPC2_peptide 7.585
IPC2.peptide.svr19 7.703
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2256
136
1970
752.0
85.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.979 ± 1.301
1.463 ± 0.558
5.053 ± 0.454
8.333 ± 0.88
2.97 ± 0.597
5.541 ± 1.422
1.995 ± 0.288
6.161 ± 0.281
6.693 ± 1.182
8.732 ± 1.71
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.261 ± 0.757
4.167 ± 0.503
5.674 ± 1.125
4.787 ± 0.779
6.738 ± 0.387
6.25 ± 0.383
4.965 ± 0.413
5.496 ± 1.167
1.418 ± 0.536
3.324 ± 0.109
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here