Escherichia phage vB_EcoS_CEB_EC3a

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Braunvirinae; Guelphvirus; Escherichia virus EC3a

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q1PWD7|A0A1Q1PWD7_9CAUD Putative membrane protein OS=Escherichia phage vB_EcoS_CEB_EC3a OX=1933774 GN=Ec3a_04 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.39VYY4 pKa = 10.88LLNGYY9 pKa = 6.93TNYY12 pKa = 10.94DD13 pKa = 3.08GFEE16 pKa = 3.9IFGVFDD22 pKa = 3.72TEE24 pKa = 3.89EE25 pKa = 3.72LAEE28 pKa = 4.11FEE30 pKa = 4.67KK31 pKa = 11.11NRR33 pKa = 11.84LLDD36 pKa = 3.34IDD38 pKa = 3.61ITLGYY43 pKa = 9.84VPDD46 pKa = 3.82YY47 pKa = 11.07YY48 pKa = 10.89LIEE51 pKa = 4.26EE52 pKa = 4.67FEE54 pKa = 4.64VKK56 pKa = 10.33VAII59 pKa = 4.76

Molecular weight: 6.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q1PWB0|A0A1Q1PWB0_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_CEB_EC3a OX=1933774 GN=Ec3a_26 PE=4 SV=1
MM1 pKa = 7.18NAVDD5 pKa = 4.57KK6 pKa = 10.67RR7 pKa = 11.84TINGNNGTIRR17 pKa = 11.84TEE19 pKa = 4.03DD20 pKa = 2.82KK21 pKa = 10.5KK22 pKa = 10.72QRR24 pKa = 11.84KK25 pKa = 8.53RR26 pKa = 11.84PSGYY30 pKa = 10.06YY31 pKa = 8.95VLKK34 pKa = 10.9DD35 pKa = 3.51EE36 pKa = 4.52VRR38 pKa = 11.84AGLRR42 pKa = 11.84ARR44 pKa = 11.84LEE46 pKa = 3.84IVLDD50 pKa = 3.88FFGTKK55 pKa = 10.47ANIAKK60 pKa = 9.21QLKK63 pKa = 7.58VTPQAVEE70 pKa = 3.72EE71 pKa = 4.24WFKK74 pKa = 11.38RR75 pKa = 11.84GMISARR81 pKa = 11.84GAQLSHH87 pKa = 6.72NYY89 pKa = 9.66YY90 pKa = 10.0KK91 pKa = 10.9RR92 pKa = 11.84NGEE95 pKa = 3.9GFRR98 pKa = 11.84ATFCRR103 pKa = 11.84PDD105 pKa = 3.38LQFDD109 pKa = 4.36GNGKK113 pKa = 8.92PLTLRR118 pKa = 11.84CKK120 pKa = 9.49KK121 pKa = 9.98RR122 pKa = 11.84HH123 pKa = 4.86MLRR126 pKa = 11.84VVTEE130 pKa = 4.33AEE132 pKa = 4.14LATKK136 pKa = 9.16PEE138 pKa = 4.04CRR140 pKa = 11.84SWRR143 pKa = 11.84KK144 pKa = 9.4IKK146 pKa = 10.36AANEE150 pKa = 3.74AARR153 pKa = 11.84KK154 pKa = 9.56AKK156 pKa = 9.88EE157 pKa = 3.61

Molecular weight: 18.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13336

36

1138

190.5

21.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.481 ± 0.474

1.222 ± 0.167

6.044 ± 0.247

6.756 ± 0.37

4.019 ± 0.221

7.543 ± 0.336

1.44 ± 0.186

6.756 ± 0.222

6.659 ± 0.497

7.086 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.557 ± 0.271

5.249 ± 0.362

3.007 ± 0.199

3.772 ± 0.35

4.634 ± 0.201

6.786 ± 0.296

5.759 ± 0.305

7.086 ± 0.254

1.222 ± 0.11

3.922 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski