Aureitalea marina
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2645 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S7KSY9|A0A2S7KSY9_9FLAO Uncharacterized protein OS=Aureitalea marina OX=930804 GN=BST85_12915 PE=4 SV=1
MM1 pKa = 7.71 KK2 pKa = 10.25 LQLSLLFVLALISSGVSAQQVSSEE26 pKa = 4.05 GAFYY30 pKa = 9.74 TGQVASMRR38 pKa = 11.84 YY39 pKa = 9.22 VEE41 pKa = 4.5 PLSSRR46 pKa = 11.84 TNLIPAEE53 pKa = 4.27 DD54 pKa = 3.83 RR55 pKa = 11.84 TGEE58 pKa = 4.08 AQDD61 pKa = 3.91 GRR63 pKa = 11.84 SSRR66 pKa = 11.84 PEE68 pKa = 3.97 VIPGKK73 pKa = 10.29 DD74 pKa = 3.16 PQTTNDD80 pKa = 3.52 YY81 pKa = 9.97 FASNPHH87 pKa = 6.56 PMTGKK92 pKa = 9.54 IASRR96 pKa = 11.84 SPLIVFDD103 pKa = 5.03 ASASSSQPTDD113 pKa = 3.16 PSLAVGPNHH122 pKa = 6.66 VISVFNTGFVIFNDD136 pKa = 3.86 KK137 pKa = 10.75 LDD139 pKa = 4.21 PANNNTGQLSVTNIFSGGGCCDD161 pKa = 3.2 LTVSYY166 pKa = 11.07 DD167 pKa = 3.33 SAADD171 pKa = 3.63 RR172 pKa = 11.84 WVLSYY177 pKa = 11.26 LFVGAGAEE185 pKa = 4.18 IAVSDD190 pKa = 4.53 GPDD193 pKa = 3.41 PNTAGWYY200 pKa = 9.62 VYY202 pKa = 10.16 SIPQINDD209 pKa = 3.22 YY210 pKa = 11.02 QKK212 pKa = 11.18 LSVWSDD218 pKa = 2.88 GYY220 pKa = 11.46 YY221 pKa = 9.04 ITDD224 pKa = 3.25 NVGGSNRR231 pKa = 11.84 VWALEE236 pKa = 3.66 RR237 pKa = 11.84 DD238 pKa = 3.62 ALLAGDD244 pKa = 4.62 PAASIQGFNLPGIVTSGFFSPQALNVTDD272 pKa = 5.46 DD273 pKa = 3.87 NMPADD278 pKa = 4.09 GNATIIYY285 pKa = 8.97 LQDD288 pKa = 4.04 DD289 pKa = 3.62 AWSGVSFDD297 pKa = 4.68 HH298 pKa = 6.67 FKK300 pKa = 10.33 IWTIDD305 pKa = 3.26 ADD307 pKa = 3.96 FDD309 pKa = 4.06 NPGNSTVSAPTEE321 pKa = 3.73 IATDD325 pKa = 3.63 PFISVFDD332 pKa = 3.97 GGSFSNLTQPGGGVAIDD349 pKa = 3.99 ALQATIMNQAQFRR362 pKa = 11.84 KK363 pKa = 9.39 FPAYY367 pKa = 10.51 NSALFNFVVDD377 pKa = 3.52 VDD379 pKa = 3.76 ATSGEE384 pKa = 4.24 KK385 pKa = 10.53 AGVRR389 pKa = 11.84 WFEE392 pKa = 3.86 LRR394 pKa = 11.84 QDD396 pKa = 3.65 GDD398 pKa = 3.87 GQPWTVYY405 pKa = 10.48 QEE407 pKa = 4.27 GTYY410 pKa = 9.69 TSPDD414 pKa = 3.45 TPTGGEE420 pKa = 3.59 KK421 pKa = 10.27 HH422 pKa = 6.16 AWHH425 pKa = 7.26 ASLMMDD431 pKa = 3.63 GSGNIGMGYY440 pKa = 10.03 SAMTVDD446 pKa = 4.8 PSDD449 pKa = 4.12 PNPIRR454 pKa = 11.84 VSSYY458 pKa = 7.5 YY459 pKa = 8.61 TGRR462 pKa = 11.84 YY463 pKa = 9.47 ASDD466 pKa = 3.59 PLGTMTIEE474 pKa = 4.26 EE475 pKa = 4.09 EE476 pKa = 4.79 LIANGNANIPGLRR489 pKa = 11.84 YY490 pKa = 10.05 GDD492 pKa = 3.79 YY493 pKa = 11.03 SKK495 pKa = 11.02 IDD497 pKa = 3.45 IDD499 pKa = 3.91 PVNDD503 pKa = 3.2 KK504 pKa = 9.31 TFWFINEE511 pKa = 3.9 YY512 pKa = 9.77 MNSGRR517 pKa = 11.84 KK518 pKa = 8.85 NVVGVFQITPNFNNDD533 pKa = 3.21 AGVVSIDD540 pKa = 3.64 TPVTGTLSDD549 pKa = 3.85 SEE551 pKa = 4.93 EE552 pKa = 3.8 ITVTIFNYY560 pKa = 10.59 GLDD563 pKa = 4.36 PIADD567 pKa = 3.7 FDD569 pKa = 3.59 VTYY572 pKa = 10.59 QVDD575 pKa = 3.5 GGAVVTEE582 pKa = 4.39 TFNGTIEE589 pKa = 4.25 STEE592 pKa = 3.87 TAQFSFVTLADD603 pKa = 4.15 LSTVGQTYY611 pKa = 10.66 SITASTALAGDD622 pKa = 3.9 EE623 pKa = 5.25 DD624 pKa = 4.25 NGNDD628 pKa = 3.45 STTKK632 pKa = 10.1 EE633 pKa = 4.15 VTFLEE638 pKa = 4.66 PNDD641 pKa = 4.36 LGVTAITAPQSGTNLPADD659 pKa = 3.68 AEE661 pKa = 4.33 ITVTITNFGGEE672 pKa = 4.29 SQSNFDD678 pKa = 2.99 VTYY681 pKa = 10.69 NLDD684 pKa = 3.61 GNEE687 pKa = 3.84 VTEE690 pKa = 4.39 QVAGPLAGNSEE701 pKa = 4.27 LSYY704 pKa = 10.94 TFTQTADD711 pKa = 3.22 LSAFGSYY718 pKa = 10.32 SLSARR723 pKa = 11.84 TDD725 pKa = 3.32 LPTDD729 pKa = 3.36 SDD731 pKa = 3.74 NTNDD735 pKa = 3.36 EE736 pKa = 4.31 TAVTIVNSNCEE747 pKa = 3.85 PGANCTLGDD756 pKa = 4.22 GIVRR760 pKa = 11.84 LQLEE764 pKa = 4.38 NVDD767 pKa = 4.26 NNSGCDD773 pKa = 3.33 PDD775 pKa = 5.94 GYY777 pKa = 11.65 GNYY780 pKa = 9.4 TNLIVEE786 pKa = 5.23 LPTPDD791 pKa = 4.2 ADD793 pKa = 3.85 YY794 pKa = 11.48 DD795 pKa = 3.9 LTITTGYY802 pKa = 10.46 GNQFVRR808 pKa = 11.84 AWIDD812 pKa = 3.48 FNDD815 pKa = 4.09 DD816 pKa = 3.41 FVFTIDD822 pKa = 3.49 EE823 pKa = 4.34 LVVNNYY829 pKa = 9.61 EE830 pKa = 4.01 IADD833 pKa = 3.82 GQGAGDD839 pKa = 3.73 YY840 pKa = 9.83 TEE842 pKa = 4.68 TMTLSVPASASPGQHH857 pKa = 6.23 LMRR860 pKa = 11.84 VKK862 pKa = 9.92 TNWNAPVPDD871 pKa = 4.61 DD872 pKa = 3.71 ACEE875 pKa = 3.71 EE876 pKa = 4.23 TTYY879 pKa = 11.58 GEE881 pKa = 4.34 TEE883 pKa = 4.59 DD884 pKa = 3.92 YY885 pKa = 10.56 LAEE888 pKa = 4.78 IGVLSFGDD896 pKa = 3.56 NQVSSEE902 pKa = 4.21 DD903 pKa = 3.71 LVIIHH908 pKa = 6.17 QGNNLFEE915 pKa = 4.36 VTMISPNGPEE925 pKa = 3.89 KK926 pKa = 11.21 LEE928 pKa = 3.94 LSVYY932 pKa = 10.53 NILGQQILNRR942 pKa = 11.84 RR943 pKa = 11.84 LEE945 pKa = 4.61 SNNGTYY951 pKa = 10.46 NYY953 pKa = 9.04 QLNMNYY959 pKa = 9.61 VSSGVYY965 pKa = 7.83 MVRR968 pKa = 11.84 LGTSQGGLVKK978 pKa = 10.53 KK979 pKa = 10.54 IVVRR983 pKa = 4.19
Molecular weight: 105.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.783
IPC_protein 3.834
Toseland 3.605
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.745
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.77
Nozaki 3.923
DTASelect 4.19
Thurlkill 3.643
EMBOSS 3.757
Sillero 3.948
Patrickios 1.074
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A2S7KPT9|A0A2S7KPT9_9FLAO Uncharacterized protein OS=Aureitalea marina OX=930804 GN=BST85_06865 PE=3 SV=1
MM1 pKa = 7.66 ISDD4 pKa = 4.0 GTNRR8 pKa = 11.84 RR9 pKa = 11.84 STVLLILGLLLCFLANISAGSVHH32 pKa = 6.49 VPLEE36 pKa = 4.31 DD37 pKa = 2.96 VWQGLIGGEE46 pKa = 4.23 TSRR49 pKa = 11.84 PAYY52 pKa = 10.16 RR53 pKa = 11.84 FIILEE58 pKa = 3.78 YY59 pKa = 9.87 RR60 pKa = 11.84 LPKK63 pKa = 10.46 AITAVLTGSGLAVSGLLMQTLFRR86 pKa = 11.84 NPLAGPFVLGLSSGASLGVAILILGAGTFGGWLGGLLLSNWSLVLASALGSFLVLMAVLAVTLRR150 pKa = 11.84 VKK152 pKa = 10.24 DD153 pKa = 3.57 TMAILIIGLMFGSVTASVVGVLSYY177 pKa = 9.09 FSPAEE182 pKa = 3.67 QLQQYY187 pKa = 7.02 VFWSFGSLGNLSWQALGVLTLCWLFGMILAIGRR220 pKa = 11.84 IKK222 pKa = 10.73 SLNSLLLGTRR232 pKa = 11.84 YY233 pKa = 9.38 AQSMGLRR240 pKa = 11.84 VRR242 pKa = 11.84 RR243 pKa = 11.84 TTLLLIVATSLLAGGITAFAGPIAFVGLAVPHH275 pKa = 6.46 LTRR278 pKa = 11.84 QLVPSADD285 pKa = 3.35 HH286 pKa = 6.94 RR287 pKa = 11.84 LLLPAVVLFGAALMLICDD305 pKa = 5.37 TIAQLPGSEE314 pKa = 3.99 FTLPINAITSLVGAPVVIWLLVRR337 pKa = 11.84 KK338 pKa = 9.59 RR339 pKa = 11.84 KK340 pKa = 10.16 LMFF343 pKa = 4.27
Molecular weight: 36.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 9.648
IPC_protein 10.496
Toseland 10.394
ProMoST 10.175
Dawson 10.57
Bjellqvist 10.321
Wikipedia 10.804
Rodwell 10.716
Grimsley 10.643
Solomon 10.657
Lehninger 10.613
Nozaki 10.423
DTASelect 10.306
Thurlkill 10.438
EMBOSS 10.804
Sillero 10.496
Patrickios 10.394
IPC_peptide 10.657
IPC2_peptide 9.516
IPC2.peptide.svr19 8.463
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2645
0
2645
881571
38
4674
333.3
37.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.87 ± 0.043
0.711 ± 0.015
6.151 ± 0.052
6.647 ± 0.048
4.869 ± 0.04
7.212 ± 0.053
1.805 ± 0.027
6.985 ± 0.035
5.64 ± 0.062
9.634 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.476 ± 0.027
4.995 ± 0.041
3.825 ± 0.028
4.089 ± 0.029
4.574 ± 0.043
6.429 ± 0.037
5.365 ± 0.057
6.588 ± 0.045
1.232 ± 0.017
3.902 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here