Amino acid dipepetide frequency for Kryptolebias marmoratus (Mangrove killifish) (Rivulus marmoratus)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.498AlaAla: 6.498 ± 0.034	1.29AlaCys: 1.29 ± 0.01	3.2AlaAsp: 3.2 ± 0.013	4.635AlaGlu: 4.635 ± 0.025	2.443AlaPhe: 2.443 ± 0.013	4.335AlaGly: 4.335 ± 0.021	1.478AlaHis: 1.478 ± 0.01	2.607AlaIle: 2.607 ± 0.013	3.316AlaLys: 3.316 ± 0.018	6.384AlaLeu: 6.384 ± 0.027	1.509AlaMet: 1.509 ± 0.011	2.15AlaAsn: 2.15 ± 0.012	3.505AlaPro: 3.505 ± 0.023	2.872AlaGln: 2.872 ± 0.018	3.103AlaArg: 3.103 ± 0.015	5.494AlaSer: 5.494 ± 0.022	3.33AlaThr: 3.33 ± 0.014	4.876AlaVal: 4.876 ± 0.022	0.635AlaTrp: 0.635 ± 0.007	1.47AlaTyr: 1.47 ± 0.009	0.0AlaXaa: 0.0 ± 0.0
Cys	1.183CysAla: 1.183 ± 0.01	0.655CysCys: 0.655 ± 0.008	1.13CysAsp: 1.13 ± 0.012	1.275CysGlu: 1.275 ± 0.014	0.969CysPhe: 0.969 ± 0.01	1.586CysGly: 1.586 ± 0.017	0.647CysHis: 0.647 ± 0.007	0.952CysIle: 0.952 ± 0.01	1.148CysLys: 1.148 ± 0.009	2.204CysLeu: 2.204 ± 0.014	0.455CysMet: 0.455 ± 0.005	0.842CysAsn: 0.842 ± 0.008	1.301CysPro: 1.301 ± 0.013	1.039CysGln: 1.039 ± 0.012	1.314CysArg: 1.314 ± 0.009	2.222CysSer: 2.222 ± 0.015	1.146CysThr: 1.146 ± 0.011	1.521CysVal: 1.521 ± 0.013	0.308CysTrp: 0.308 ± 0.005	0.608CysTyr: 0.608 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	2.924AspAla: 2.924 ± 0.015	1.177AspCys: 1.177 ± 0.011	3.072AspAsp: 3.072 ± 0.021	3.825AspGlu: 3.825 ± 0.021	2.171AspPhe: 2.171 ± 0.013	3.708AspGly: 3.708 ± 0.021	1.196AspHis: 1.196 ± 0.009	2.61AspIle: 2.61 ± 0.014	2.733AspLys: 2.733 ± 0.014	5.008AspLeu: 5.008 ± 0.022	1.252AspMet: 1.252 ± 0.009	1.938AspAsn: 1.938 ± 0.015	2.872AspPro: 2.872 ± 0.015	1.946AspGln: 1.946 ± 0.012	2.818AspArg: 2.818 ± 0.015	4.562AspSer: 4.562 ± 0.021	2.55AspThr: 2.55 ± 0.014	3.36AspVal: 3.36 ± 0.014	0.669AspTrp: 0.669 ± 0.007	1.532AspTyr: 1.532 ± 0.01	0.0AspXaa: 0.0 ± 0.0
Glu	4.681GluAla: 4.681 ± 0.023	1.192GluCys: 1.192 ± 0.013	4.467GluAsp: 4.467 ± 0.02	7.767GluGlu: 7.767 ± 0.055	1.963GluPhe: 1.963 ± 0.013	4.002GluGly: 4.002 ± 0.018	1.45GluHis: 1.45 ± 0.01	2.845GluIle: 2.845 ± 0.016	4.723GluLys: 4.723 ± 0.028	6.153GluLeu: 6.153 ± 0.029	1.709GluMet: 1.709 ± 0.012	2.836GluAsn: 2.836 ± 0.016	2.908GluPro: 2.908 ± 0.016	3.078GluGln: 3.078 ± 0.02	4.221GluArg: 4.221 ± 0.028	4.38GluSer: 4.38 ± 0.02	3.465GluThr: 3.465 ± 0.02	4.247GluVal: 4.247 ± 0.02	0.672GluTrp: 0.672 ± 0.006	1.561GluTyr: 1.561 ± 0.01	0.0GluXaa: 0.0 ± 0.0
Phe	1.964PheAla: 1.964 ± 0.012	1.043PheCys: 1.043 ± 0.008	1.817PheAsp: 1.817 ± 0.012	1.902PheGlu: 1.902 ± 0.013	1.727PhePhe: 1.727 ± 0.014	2.267PheGly: 2.267 ± 0.016	1.041PheHis: 1.041 ± 0.008	1.978PheIle: 1.978 ± 0.013	1.886PheLys: 1.886 ± 0.011	4.051PheLeu: 4.051 ± 0.02	0.834PheMet: 0.834 ± 0.007	1.525PheAsn: 1.525 ± 0.011	1.858PhePro: 1.858 ± 0.012	1.662PheGln: 1.662 ± 0.011	1.943PheArg: 1.943 ± 0.011	3.596PheSer: 3.596 ± 0.017	2.304PheThr: 2.304 ± 0.016	2.355PheVal: 2.355 ± 0.014	0.498PheTrp: 0.498 ± 0.006	1.276PheTyr: 1.276 ± 0.01	0.0PheXaa: 0.0 ± 0.0
Gly	4.033GlyAla: 4.033 ± 0.022	1.249GlyCys: 1.249 ± 0.011	3.227GlyAsp: 3.227 ± 0.018	4.014GlyGlu: 4.014 ± 0.023	2.497GlyPhe: 2.497 ± 0.016	5.427GlyGly: 5.427 ± 0.037	1.651GlyHis: 1.651 ± 0.013	2.504GlyIle: 2.504 ± 0.014	3.491GlyLys: 3.491 ± 0.016	5.423GlyLeu: 5.423 ± 0.019	1.417GlyMet: 1.417 ± 0.011	2.433GlyAsn: 2.433 ± 0.014	3.277GlyPro: 3.277 ± 0.036	2.71GlyGln: 2.71 ± 0.017	3.63GlyArg: 3.63 ± 0.022	5.839GlySer: 5.839 ± 0.027	3.337GlyThr: 3.337 ± 0.018	3.98GlyVal: 3.98 ± 0.019	0.774GlyTrp: 0.774 ± 0.008	1.796GlyTyr: 1.796 ± 0.013	0.001GlyXaa: 0.001 ± 0.0
His	1.322HisAla: 1.322 ± 0.009	0.729HisCys: 0.729 ± 0.008	0.959HisAsp: 0.959 ± 0.008	1.215HisGlu: 1.215 ± 0.009	1.107HisPhe: 1.107 ± 0.008	1.562HisGly: 1.562 ± 0.013	1.103HisHis: 1.103 ± 0.016	1.295HisIle: 1.295 ± 0.009	1.32HisLys: 1.32 ± 0.009	2.768HisLeu: 2.768 ± 0.015	0.673HisMet: 0.673 ± 0.008	1.016HisAsn: 1.016 ± 0.008	1.632HisPro: 1.632 ± 0.013	1.281HisGln: 1.281 ± 0.012	1.657HisArg: 1.657 ± 0.012	2.439HisSer: 2.439 ± 0.015	1.565HisThr: 1.565 ± 0.012	1.463HisVal: 1.463 ± 0.01	0.327HisTrp: 0.327 ± 0.005	0.848HisTyr: 0.848 ± 0.008	0.0HisXaa: 0.0 ± 0.0
Ile	2.382IleAla: 2.382 ± 0.013	1.095IleCys: 1.095 ± 0.009	2.043IleAsp: 2.043 ± 0.013	2.329IleGlu: 2.329 ± 0.014	1.857IlePhe: 1.857 ± 0.012	2.258IleGly: 2.258 ± 0.013	1.279IleHis: 1.279 ± 0.009	2.432IleIle: 2.432 ± 0.016	2.571IleLys: 2.571 ± 0.014	4.284IleLeu: 4.284 ± 0.021	1.065IleMet: 1.065 ± 0.009	2.01IleAsn: 2.01 ± 0.013	2.421IlePro: 2.421 ± 0.012	2.184IleGln: 2.184 ± 0.013	2.436IleArg: 2.436 ± 0.012	3.804IleSer: 3.804 ± 0.015	2.721IleThr: 2.721 ± 0.016	2.556IleVal: 2.556 ± 0.015	0.477IleTrp: 0.477 ± 0.005	1.4IleTyr: 1.4 ± 0.011	0.0IleXaa: 0.0 ± 0.0
Lys	3.679LysAla: 3.679 ± 0.017	1.051LysCys: 1.051 ± 0.01	3.276LysAsp: 3.276 ± 0.018	4.707LysGlu: 4.707 ± 0.026	1.643LysPhe: 1.643 ± 0.01	3.143LysGly: 3.143 ± 0.023	1.448LysHis: 1.448 ± 0.011	2.501LysIle: 2.501 ± 0.014	4.475LysLys: 4.475 ± 0.026	5.054LysLeu: 5.054 ± 0.021	1.494LysMet: 1.494 ± 0.011	2.35LysAsn: 2.35 ± 0.014	2.987LysPro: 2.987 ± 0.016	2.601LysGln: 2.601 ± 0.016	3.401LysArg: 3.401 ± 0.019	3.908LysSer: 3.908 ± 0.02	3.319LysThr: 3.319 ± 0.019	3.535LysVal: 3.535 ± 0.016	0.58LysTrp: 0.58 ± 0.006	1.481LysTyr: 1.481 ± 0.01	0.0LysXaa: 0.0 ± 0.0
Leu	5.919LeuAla: 5.919 ± 0.027	2.248LeuCys: 2.248 ± 0.015	4.893LeuAsp: 4.893 ± 0.022	6.333LeuGlu: 6.333 ± 0.032	3.568LeuPhe: 3.568 ± 0.019	5.092LeuGly: 5.092 ± 0.018	2.701LeuHis: 2.701 ± 0.016	3.865LeuIle: 3.865 ± 0.018	5.687LeuLys: 5.687 ± 0.023	10.136LeuLeu: 10.136 ± 0.042	2.129LeuMet: 2.129 ± 0.013	3.74LeuAsn: 3.74 ± 0.017	5.347LeuPro: 5.347 ± 0.021	5.531LeuGln: 5.531 ± 0.028	5.664LeuArg: 5.664 ± 0.022	8.486LeuSer: 8.486 ± 0.031	5.262LeuThr: 5.262 ± 0.02	5.474LeuVal: 5.474 ± 0.026	1.059LeuTrp: 1.059 ± 0.011	2.611LeuTyr: 2.611 ± 0.015	0.0LeuXaa: 0.0 ± 0.0
Met	1.853MetAla: 1.853 ± 0.011	0.475MetCys: 0.475 ± 0.006	1.397MetAsp: 1.397 ± 0.008	1.965MetGlu: 1.965 ± 0.012	0.884MetPhe: 0.884 ± 0.008	1.372MetGly: 1.372 ± 0.01	0.476MetHis: 0.476 ± 0.006	0.894MetIle: 0.894 ± 0.008	1.503MetLys: 1.503 ± 0.011	2.065MetLeu: 2.065 ± 0.012	0.701MetMet: 0.701 ± 0.007	0.911MetAsn: 0.911 ± 0.007	1.062MetPro: 1.062 ± 0.01	0.945MetGln: 0.945 ± 0.008	1.165MetArg: 1.165 ± 0.009	1.899MetSer: 1.899 ± 0.011	1.253MetThr: 1.253 ± 0.008	1.521MetVal: 1.521 ± 0.01	0.258MetTrp: 0.258 ± 0.004	0.617MetTyr: 0.617 ± 0.006	0.0MetXaa: 0.0 ± 0.0
Asn	2.124AsnAla: 2.124 ± 0.012	0.854AsnCys: 0.854 ± 0.009	1.701AsnAsp: 1.701 ± 0.014	2.119AsnGlu: 2.119 ± 0.011	1.481AsnPhe: 1.481 ± 0.011	2.763AsnGly: 2.763 ± 0.02	1.035AsnHis: 1.035 ± 0.008	2.189AsnIle: 2.189 ± 0.014	2.351AsnLys: 2.351 ± 0.013	3.715AsnLeu: 3.715 ± 0.02	1.068AsnMet: 1.068 ± 0.008	1.899AsnAsn: 1.899 ± 0.013	2.287AsnPro: 2.287 ± 0.014	1.827AsnGln: 1.827 ± 0.01	2.024AsnArg: 2.024 ± 0.012	3.331AsnSer: 3.331 ± 0.017	2.245AsnThr: 2.245 ± 0.013	2.327AsnVal: 2.327 ± 0.015	0.455AsnTrp: 0.455 ± 0.005	1.155AsnTyr: 1.155 ± 0.009	0.0AsnXaa: 0.0 ± 0.0
Pro	4.223ProAla: 4.223 ± 0.026	1.069ProCys: 1.069 ± 0.011	2.94ProAsp: 2.94 ± 0.017	3.798ProGlu: 3.798 ± 0.019	1.882ProPhe: 1.882 ± 0.012	4.03ProGly: 4.03 ± 0.046	1.513ProHis: 1.513 ± 0.012	1.855ProIle: 1.855 ± 0.011	2.57ProLys: 2.57 ± 0.018	4.893ProLeu: 4.893 ± 0.021	1.006ProMet: 1.006 ± 0.01	1.978ProAsn: 1.978 ± 0.013	5.671ProPro: 5.671 ± 0.054	2.708ProGln: 2.708 ± 0.022	2.776ProArg: 2.776 ± 0.015	5.689ProSer: 5.689 ± 0.032	3.04ProThr: 3.04 ± 0.019	3.78ProVal: 3.78 ± 0.019	0.543ProTrp: 0.543 ± 0.006	1.411ProTyr: 1.411 ± 0.012	0.002ProXaa: 0.002 ± 0.0
Gln	3.107GlnAla: 3.107 ± 0.018	0.926GlnCys: 0.926 ± 0.01	2.337GlnAsp: 2.337 ± 0.013	3.533GlnGlu: 3.533 ± 0.02	1.394GlnPhe: 1.394 ± 0.01	2.586GlnGly: 2.586 ± 0.018	1.354GlnHis: 1.354 ± 0.011	1.982GlnIle: 1.982 ± 0.01	2.665GlnLys: 2.665 ± 0.015	4.516GlnLeu: 4.516 ± 0.023	1.152GlnMet: 1.152 ± 0.009	1.904GlnAsn: 1.904 ± 0.011	2.586GlnPro: 2.586 ± 0.018	3.344GlnGln: 3.344 ± 0.035	3.047GlnArg: 3.047 ± 0.016	3.523GlnSer: 3.523 ± 0.017	2.669GlnThr: 2.669 ± 0.015	2.818GlnVal: 2.818 ± 0.016	0.529GlnTrp: 0.529 ± 0.006	1.197GlnTyr: 1.197 ± 0.009	0.0GlnXaa: 0.0 ± 0.0
Arg	3.422ArgAla: 3.422 ± 0.019	1.282ArgCys: 1.282 ± 0.012	2.95ArgAsp: 2.95 ± 0.016	3.835ArgGlu: 3.835 ± 0.022	2.05ArgPhe: 2.05 ± 0.011	3.446ArgGly: 3.446 ± 0.022	1.577ArgHis: 1.577 ± 0.01	2.382ArgIle: 2.382 ± 0.012	3.555ArgLys: 3.555 ± 0.017	5.289ArgLeu: 5.289 ± 0.023	1.271ArgMet: 1.271 ± 0.01	2.151ArgAsn: 2.151 ± 0.012	2.954ArgPro: 2.954 ± 0.02	2.653ArgGln: 2.653 ± 0.014	4.483ArgArg: 4.483 ± 0.026	4.751ArgSer: 4.751 ± 0.025	3.022ArgThr: 3.022 ± 0.016	3.254ArgVal: 3.254 ± 0.016	0.69ArgTrp: 0.69 ± 0.007	1.537ArgTyr: 1.537 ± 0.009	0.0ArgXaa: 0.0 ± 0.0
Ser	5.774SerAla: 5.774 ± 0.025	2.108SerCys: 2.108 ± 0.018	4.469SerAsp: 4.469 ± 0.02	5.069SerGlu: 5.069 ± 0.025	3.264SerPhe: 3.264 ± 0.016	5.678SerGly: 5.678 ± 0.024	2.274SerHis: 2.274 ± 0.015	3.309SerIle: 3.309 ± 0.015	4.129SerLys: 4.129 ± 0.017	8.447SerLeu: 8.447 ± 0.03	1.783SerMet: 1.783 ± 0.01	3.081SerAsn: 3.081 ± 0.018	6.092SerPro: 6.092 ± 0.037	3.953SerGln: 3.953 ± 0.019	4.662SerArg: 4.662 ± 0.024	10.881SerSer: 10.881 ± 0.063	4.737SerThr: 4.737 ± 0.025	5.597SerVal: 5.597 ± 0.02	1.024SerTrp: 1.024 ± 0.008	2.185SerTyr: 2.185 ± 0.014	0.001SerXaa: 0.001 ± 0.0
Thr	3.897ThrAla: 3.897 ± 0.018	1.371ThrCys: 1.371 ± 0.016	2.865ThrAsp: 2.865 ± 0.015	3.719ThrGlu: 3.719 ± 0.019	2.183ThrPhe: 2.183 ± 0.014	3.641ThrGly: 3.641 ± 0.018	1.394ThrHis: 1.394 ± 0.011	2.391ThrIle: 2.391 ± 0.016	2.732ThrLys: 2.732 ± 0.016	5.277ThrLeu: 5.277 ± 0.023	1.187ThrMet: 1.187 ± 0.01	2.012ThrAsn: 2.012 ± 0.013	3.575ThrPro: 3.575 ± 0.024	2.347ThrGln: 2.347 ± 0.014	2.507ThrArg: 2.507 ± 0.011	4.919ThrSer: 4.919 ± 0.025	3.297ThrThr: 3.297 ± 0.027	4.08ThrVal: 4.08 ± 0.022	0.653ThrTrp: 0.653 ± 0.006	1.427ThrTyr: 1.427 ± 0.012	0.001ThrXaa: 0.001 ± 0.0
Val	4.079ValAla: 4.079 ± 0.016	1.739ValCys: 1.739 ± 0.013	3.179ValAsp: 3.179 ± 0.016	3.964ValGlu: 3.964 ± 0.018	2.739ValPhe: 2.739 ± 0.017	3.546ValGly: 3.546 ± 0.018	1.604ValHis: 1.604 ± 0.011	2.999ValIle: 2.999 ± 0.017	3.57ValLys: 3.57 ± 0.016	6.311ValLeu: 6.311 ± 0.028	1.515ValMet: 1.515 ± 0.011	2.454ValAsn: 2.454 ± 0.014	3.316ValPro: 3.316 ± 0.016	2.772ValGln: 2.772 ± 0.013	3.291ValArg: 3.291 ± 0.017	5.463ValSer: 5.463 ± 0.022	3.901ValThr: 3.901 ± 0.022	4.391ValVal: 4.391 ± 0.023	0.781ValTrp: 0.781 ± 0.007	1.788ValTyr: 1.788 ± 0.012	0.0ValXaa: 0.0 ± 0.0
Trp	0.671TrpAla: 0.671 ± 0.007	0.246TrpCys: 0.246 ± 0.004	0.63TrpAsp: 0.63 ± 0.007	0.738TrpGlu: 0.738 ± 0.007	0.476TrpPhe: 0.476 ± 0.006	0.623TrpGly: 0.623 ± 0.007	0.262TrpHis: 0.262 ± 0.004	0.568TrpIle: 0.568 ± 0.007	0.719TrpLys: 0.719 ± 0.006	1.145TrpLeu: 1.145 ± 0.01	0.344TrpMet: 0.344 ± 0.005	0.502TrpAsn: 0.502 ± 0.005	0.429TrpPro: 0.429 ± 0.005	0.482TrpGln: 0.482 ± 0.006	0.743TrpArg: 0.743 ± 0.007	0.963TrpSer: 0.963 ± 0.01	0.727TrpThr: 0.727 ± 0.007	0.699TrpVal: 0.699 ± 0.008	0.194TrpTrp: 0.194 ± 0.004	0.338TrpTyr: 0.338 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.395TyrAla: 1.395 ± 0.01	0.706TyrCys: 0.706 ± 0.007	1.372TyrAsp: 1.372 ± 0.011	1.583TyrGlu: 1.583 ± 0.012	1.227TyrPhe: 1.227 ± 0.01	1.645TyrGly: 1.645 ± 0.011	0.799TyrHis: 0.799 ± 0.007	1.445TyrIle: 1.445 ± 0.01	1.489TyrLys: 1.489 ± 0.01	2.622TyrLeu: 2.622 ± 0.015	0.652TyrMet: 0.652 ± 0.006	1.218TyrAsn: 1.218 ± 0.009	1.295TyrPro: 1.295 ± 0.011	1.232TyrGln: 1.232 ± 0.009	1.669TyrArg: 1.669 ± 0.011	2.367TyrSer: 2.367 ± 0.015	1.583TyrThr: 1.583 ± 0.011	1.567TyrVal: 1.567 ± 0.01	0.374TyrTrp: 0.374 ± 0.006	0.973TyrTyr: 0.973 ± 0.008	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.234XaaXaa: 0.234 ± 0.042

Statistics based on 29686 proteins (17386265 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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