Tick-associated genomovirus 3
Average proteome isoelectric point is 7.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D0Y219|A0A2D0Y219_9VIRU Capsid protein OS=Tick-associated genomovirus 3 OX=2025479 PE=4 SV=1
MM1 pKa = 7.52 LFCNSKK7 pKa = 10.7 YY8 pKa = 11.1 VLLTYY13 pKa = 9.13 AQCGDD18 pKa = 3.67 LDD20 pKa = 3.59 EE21 pKa = 4.86 WAVSDD26 pKa = 4.61 HH27 pKa = 6.68 LSSLDD32 pKa = 3.46 AEE34 pKa = 4.68 CIVARR39 pKa = 11.84 EE40 pKa = 4.05 IHH42 pKa = 6.23 PTTGGVHH49 pKa = 5.86 LHH51 pKa = 5.79 VFVDD55 pKa = 4.54 FGRR58 pKa = 11.84 KK59 pKa = 7.9 FRR61 pKa = 11.84 SRR63 pKa = 11.84 RR64 pKa = 11.84 VDD66 pKa = 3.24 VFDD69 pKa = 4.17 VEE71 pKa = 4.89 GRR73 pKa = 11.84 HH74 pKa = 5.94 PNVVPSKK81 pKa = 8.26 GTPEE85 pKa = 3.8 KK86 pKa = 10.85 GYY88 pKa = 10.88 DD89 pKa = 3.43 YY90 pKa = 10.61 AIKK93 pKa = 10.61 DD94 pKa = 3.82 GEE96 pKa = 4.58 VVAGGLARR104 pKa = 11.84 PRR106 pKa = 11.84 GGRR109 pKa = 11.84 PGTLSGLEE117 pKa = 4.48 AIAHH121 pKa = 5.74 LCEE124 pKa = 4.35 TQDD127 pKa = 3.61 EE128 pKa = 4.36 FLDD131 pKa = 3.72 IYY133 pKa = 11.48 GEE135 pKa = 3.96 VDD137 pKa = 2.94 TRR139 pKa = 11.84 GLIKK143 pKa = 10.77 NFANVRR149 pKa = 11.84 SYY151 pKa = 11.76 AKK153 pKa = 9.1 WRR155 pKa = 11.84 YY156 pKa = 9.85 ASTLPKK162 pKa = 10.15 YY163 pKa = 9.53 EE164 pKa = 4.28 SPASFGEE171 pKa = 3.93 FRR173 pKa = 11.84 GGSDD177 pKa = 5.89 GRR179 pKa = 11.84 DD180 pKa = 2.8 QWLAQSGIRR189 pKa = 11.84 SNDD192 pKa = 2.91 VVRR195 pKa = 11.84 PKK197 pKa = 10.96 SLVLYY202 pKa = 10.16 GPSRR206 pKa = 11.84 TGKK209 pKa = 8.43 TSWARR214 pKa = 11.84 SLGPHH219 pKa = 6.33 VYY221 pKa = 10.46 FGGAFSGGDD230 pKa = 3.53 ALAVDD235 pKa = 5.33 DD236 pKa = 4.41 DD237 pKa = 4.09 VKK239 pKa = 11.4 YY240 pKa = 11.03 AVFDD244 pKa = 4.0 DD245 pKa = 3.88 MRR247 pKa = 11.84 GGIAFFHH254 pKa = 6.43 GWKK257 pKa = 10.0 DD258 pKa = 3.13 WLGAQQEE265 pKa = 4.97 FYY267 pKa = 10.67 GQSAVSRR274 pKa = 11.84 SKK276 pKa = 10.98 SYY278 pKa = 11.19 LSGVDD283 pKa = 3.36 HH284 pKa = 6.76 QFWCANRR291 pKa = 11.84 DD292 pKa = 3.29 PRR294 pKa = 11.84 EE295 pKa = 3.89 EE296 pKa = 4.18 MEE298 pKa = 4.04 CHH300 pKa = 6.07 LFKK303 pKa = 10.88 GTFCQGDD310 pKa = 4.1 IDD312 pKa = 4.15 WLNANCVFIEE322 pKa = 4.11 VNTPIFHH329 pKa = 7.54 ANRR332 pKa = 11.84 EE333 pKa = 4.13
Molecular weight: 37.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.615
IPC2_protein 5.588
IPC_protein 5.626
Toseland 5.995
ProMoST 5.855
Dawson 5.868
Bjellqvist 5.893
Wikipedia 5.881
Rodwell 5.855
Grimsley 6.135
Solomon 5.881
Lehninger 5.868
Nozaki 6.135
DTASelect 6.326
Thurlkill 6.326
EMBOSS 6.287
Sillero 6.237
Patrickios 3.986
IPC_peptide 5.893
IPC2_peptide 6.237
IPC2.peptide.svr19 6.196
Protein with the highest isoelectric point:
>tr|A0A2D0Y219|A0A2D0Y219_9VIRU Capsid protein OS=Tick-associated genomovirus 3 OX=2025479 PE=4 SV=1
MM1 pKa = 7.65 PRR3 pKa = 11.84 FKK5 pKa = 10.85 NRR7 pKa = 11.84 RR8 pKa = 11.84 TRR10 pKa = 11.84 YY11 pKa = 7.83 ARR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 VGRR18 pKa = 11.84 FNYY21 pKa = 9.84 GNKK24 pKa = 9.4 FSKK27 pKa = 10.64 GKK29 pKa = 10.65 GPIRR33 pKa = 11.84 RR34 pKa = 11.84 TKK36 pKa = 8.5 RR37 pKa = 11.84 TYY39 pKa = 9.79 RR40 pKa = 11.84 PKK42 pKa = 8.38 MTKK45 pKa = 10.05 KK46 pKa = 10.4 RR47 pKa = 11.84 ILNVTSKK54 pKa = 10.49 KK55 pKa = 10.46 KK56 pKa = 9.98 HH57 pKa = 5.53 DD58 pKa = 3.85 TMLTTTNISASSQYY72 pKa = 10.82 GGSAYY77 pKa = 9.42 IQAPAIITGASNAIPIVWCATARR100 pKa = 11.84 DD101 pKa = 3.6 ASYY104 pKa = 10.87 DD105 pKa = 3.5 AKK107 pKa = 10.8 LANPTDD113 pKa = 3.56 TATRR117 pKa = 11.84 TAQTCFMRR125 pKa = 11.84 GLSEE129 pKa = 4.41 KK130 pKa = 10.49 IEE132 pKa = 4.01 IQVADD137 pKa = 3.81 GMPWQWRR144 pKa = 11.84 RR145 pKa = 11.84 ICFTYY150 pKa = 10.71 RR151 pKa = 11.84 NLTTLIPNTTEE162 pKa = 3.47 AGKK165 pKa = 10.64 YY166 pKa = 8.75 FYY168 pKa = 10.82 SYY170 pKa = 11.35 LSIGNLGYY178 pKa = 10.9 NRR180 pKa = 11.84 VLNMLPHH187 pKa = 6.88 PDD189 pKa = 2.83 IVATLEE195 pKa = 4.12 SVLFRR200 pKa = 11.84 GVKK203 pKa = 10.03 GKK205 pKa = 9.93 DD206 pKa = 2.92 WQSAMIAAVDD216 pKa = 3.82 RR217 pKa = 11.84 LRR219 pKa = 11.84 VDD221 pKa = 3.85 LKK223 pKa = 10.31 YY224 pKa = 11.32 DD225 pKa = 3.3 KK226 pKa = 10.49 TITIASGNEE235 pKa = 3.56 DD236 pKa = 3.37 GVIRR240 pKa = 11.84 KK241 pKa = 8.44 YY242 pKa = 11.15 SRR244 pKa = 11.84 WHH246 pKa = 5.81 GMNKK250 pKa = 9.58 NLVYY254 pKa = 10.79 ADD256 pKa = 4.45 DD257 pKa = 4.05 EE258 pKa = 5.13 SGGEE262 pKa = 4.09 MIEE265 pKa = 4.69 AYY267 pKa = 10.4 HH268 pKa = 6.25 SVSDD272 pKa = 3.58 KK273 pKa = 11.21 RR274 pKa = 11.84 GMGDD278 pKa = 3.51 YY279 pKa = 11.07 YY280 pKa = 10.34 IVDD283 pKa = 3.77 YY284 pKa = 9.16 FAPRR288 pKa = 11.84 AGATSSNQISVGMSSTLYY306 pKa = 8.84 WHH308 pKa = 7.07 EE309 pKa = 4.1 KK310 pKa = 9.32
Molecular weight: 35.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.251
IPC2_protein 9.575
IPC_protein 9.78
Toseland 10.028
ProMoST 9.823
Dawson 10.277
Bjellqvist 9.984
Wikipedia 10.467
Rodwell 10.599
Grimsley 10.365
Solomon 10.292
Lehninger 10.248
Nozaki 10.028
DTASelect 9.97
Thurlkill 10.101
EMBOSS 10.438
Sillero 10.189
Patrickios 9.823
IPC_peptide 10.292
IPC2_peptide 8.741
IPC2.peptide.svr19 8.585
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1020
310
377
340.0
38.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.941 ± 0.189
1.961 ± 0.378
6.667 ± 0.587
4.51 ± 0.608
4.902 ± 0.881
9.804 ± 0.912
2.647 ± 0.393
4.314 ± 0.943
5.098 ± 0.761
6.667 ± 0.628
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.765 ± 0.676
3.824 ± 0.507
3.725 ± 0.076
2.745 ± 0.231
7.647 ± 0.408
7.353 ± 0.056
5.0 ± 1.298
6.667 ± 0.694
2.255 ± 0.121
4.51 ± 0.625
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here