Phytohabitans houttuyneae
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10523 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6V8K413|A0A6V8K413_9ACTN Glucosylceramidase OS=Phytohabitans houttuyneae OX=1076126 GN=srfJ PE=3 SV=1
MM1 pKa = 7.86 SEE3 pKa = 4.59 FDD5 pKa = 5.56 DD6 pKa = 4.04 PTLTEE11 pKa = 4.7 DD12 pKa = 3.99 PGTGHH17 pKa = 6.91 EE18 pKa = 4.73 GFGHH22 pKa = 6.68 AGDD25 pKa = 4.87 GGHH28 pKa = 6.33 EE29 pKa = 4.2 PGYY32 pKa = 10.02 DD33 pKa = 3.29 QPGYY37 pKa = 9.27 EE38 pKa = 4.51 QPTHH42 pKa = 6.31 DD43 pKa = 4.31 TPPYY47 pKa = 9.97 GHH49 pKa = 7.58 DD50 pKa = 3.29 EE51 pKa = 3.86 PHH53 pKa = 6.9 YY54 pKa = 9.59 EE55 pKa = 4.21 EE56 pKa = 4.67 PHH58 pKa = 7.02 HH59 pKa = 6.77 GEE61 pKa = 4.09 PDD63 pKa = 3.74 SIVQKK68 pKa = 10.96 NPDD71 pKa = 3.24 GSTDD75 pKa = 3.45 VVVDD79 pKa = 4.4 LNGDD83 pKa = 4.0 GYY85 pKa = 11.69 ADD87 pKa = 3.88 VVQHH91 pKa = 7.25 DD92 pKa = 4.47 SDD94 pKa = 4.14 SDD96 pKa = 4.11 GYY98 pKa = 11.82 ADD100 pKa = 3.53 TTYY103 pKa = 11.07 VDD105 pKa = 3.95 SNHH108 pKa = 7.24 DD109 pKa = 3.47 GRR111 pKa = 11.84 LDD113 pKa = 4.05 TILKK117 pKa = 10.57 DD118 pKa = 3.71 YY119 pKa = 11.36 DD120 pKa = 4.09 DD121 pKa = 6.47 DD122 pKa = 6.58 GDD124 pKa = 4.29 LDD126 pKa = 4.31 AAFADD131 pKa = 4.28 TNADD135 pKa = 3.06 GRR137 pKa = 11.84 VDD139 pKa = 3.53 YY140 pKa = 10.95 AAADD144 pKa = 3.97 LNDD147 pKa = 4.46 DD148 pKa = 3.57 NRR150 pKa = 11.84 VDD152 pKa = 4.84 HH153 pKa = 6.18 VTLDD157 pKa = 3.17 TDD159 pKa = 4.03 YY160 pKa = 11.57 DD161 pKa = 4.02 GKK163 pKa = 11.07 ADD165 pKa = 3.43 TWIADD170 pKa = 3.07 VDD172 pKa = 4.13 YY173 pKa = 11.23 DD174 pKa = 4.23 GKK176 pKa = 10.26 PDD178 pKa = 3.48 VAFVDD183 pKa = 4.32 TNHH186 pKa = 7.44 DD187 pKa = 3.64 GTPDD191 pKa = 3.4 VAVTDD196 pKa = 4.18 TNHH199 pKa = 7.37 DD200 pKa = 3.88 GTPEE204 pKa = 3.85 QQPVNPYY211 pKa = 9.82 AASS214 pKa = 3.26
Molecular weight: 23.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.643
IPC_protein 3.694
Toseland 3.452
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.694
Rodwell 3.516
Grimsley 3.35
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.151
Thurlkill 3.516
EMBOSS 3.694
Sillero 3.821
Patrickios 0.718
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.74
Protein with the highest isoelectric point:
>tr|A0A6V8KUQ9|A0A6V8KUQ9_9ACTN Uncharacterized protein OS=Phytohabitans houttuyneae OX=1076126 GN=Phou_084950 PE=4 SV=1
MM1 pKa = 7.36 TGTRR5 pKa = 11.84 LRR7 pKa = 11.84 RR8 pKa = 11.84 PAVPGLSVPRR18 pKa = 11.84 TGLPVARR25 pKa = 11.84 TGLPVARR32 pKa = 11.84 SGLSTAGTGLFVRR45 pKa = 11.84 RR46 pKa = 11.84 ARR48 pKa = 11.84 LPAARR53 pKa = 11.84 SWRR56 pKa = 11.84 LTSAGWRR63 pKa = 11.84 GSARR67 pKa = 11.84 RR68 pKa = 11.84 RR69 pKa = 11.84 AAFPAGPSAAGLEE82 pKa = 4.25 PLVRR86 pKa = 11.84 AAVTTRR92 pKa = 11.84 PRR94 pKa = 11.84 LVSWPAAAVAAARR107 pKa = 11.84 TRR109 pKa = 11.84 AGPPLAAA116 pKa = 5.27
Molecular weight: 11.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.512
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.457
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.179
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.199
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10523
0
10523
3370841
39
8146
320.3
34.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.207 ± 0.04
0.782 ± 0.007
5.946 ± 0.019
5.006 ± 0.025
2.74 ± 0.014
9.367 ± 0.03
2.151 ± 0.014
3.247 ± 0.016
1.634 ± 0.017
10.203 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.686 ± 0.01
1.84 ± 0.018
6.278 ± 0.023
2.73 ± 0.012
8.346 ± 0.029
4.983 ± 0.02
6.138 ± 0.025
8.886 ± 0.024
1.683 ± 0.01
2.146 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here