Amino acid dipepetide frequency for Ruminococcaceae bacterium D16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.65AlaAla: 10.65 ± 0.158	1.491AlaCys: 1.491 ± 0.045	4.922AlaAsp: 4.922 ± 0.071	5.316AlaGlu: 5.316 ± 0.097	3.292AlaPhe: 3.292 ± 0.063	7.302AlaGly: 7.302 ± 0.104	1.433AlaHis: 1.433 ± 0.046	5.224AlaIle: 5.224 ± 0.08	4.521AlaLys: 4.521 ± 0.08	10.264AlaLeu: 10.264 ± 0.123	2.802AlaMet: 2.802 ± 0.049	2.431AlaAsn: 2.431 ± 0.061	3.362AlaPro: 3.362 ± 0.063	4.478AlaGln: 4.478 ± 0.086	4.287AlaArg: 4.287 ± 0.072	4.187AlaSer: 4.187 ± 0.074	3.712AlaThr: 3.712 ± 0.069	7.481AlaVal: 7.481 ± 0.109	0.945AlaTrp: 0.945 ± 0.036	2.647AlaTyr: 2.647 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	1.488CysAla: 1.488 ± 0.04	0.327CysCys: 0.327 ± 0.022	0.836CysAsp: 0.836 ± 0.03	0.792CysGlu: 0.792 ± 0.033	0.594CysPhe: 0.594 ± 0.025	1.651CysGly: 1.651 ± 0.04	0.321CysHis: 0.321 ± 0.021	0.847CysIle: 0.847 ± 0.033	0.69CysLys: 0.69 ± 0.032	1.426CysLeu: 1.426 ± 0.045	0.386CysMet: 0.386 ± 0.019	0.481CysAsn: 0.481 ± 0.025	0.853CysPro: 0.853 ± 0.034	0.604CysGln: 0.604 ± 0.026	0.993CysArg: 0.993 ± 0.032	0.856CysSer: 0.856 ± 0.029	0.861CysThr: 0.861 ± 0.033	1.095CysVal: 1.095 ± 0.036	0.192CysTrp: 0.192 ± 0.014	0.53CysTyr: 0.53 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.696AspAla: 4.696 ± 0.077	0.863AspCys: 0.863 ± 0.032	2.734AspAsp: 2.734 ± 0.072	4.025AspGlu: 4.025 ± 0.086	2.433AspPhe: 2.433 ± 0.049	4.723AspGly: 4.723 ± 0.09	1.058AspHis: 1.058 ± 0.032	3.281AspIle: 3.281 ± 0.064	2.672AspLys: 2.672 ± 0.063	5.433AspLeu: 5.433 ± 0.086	1.548AspMet: 1.548 ± 0.039	1.758AspAsn: 1.758 ± 0.049	2.575AspPro: 2.575 ± 0.06	2.145AspGln: 2.145 ± 0.053	2.994AspArg: 2.994 ± 0.058	2.801AspSer: 2.801 ± 0.061	3.124AspThr: 3.124 ± 0.065	3.672AspVal: 3.672 ± 0.066	0.679AspTrp: 0.679 ± 0.032	2.372AspTyr: 2.372 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	5.709GluAla: 5.709 ± 0.092	0.72GluCys: 0.72 ± 0.028	3.91GluAsp: 3.91 ± 0.073	5.938GluGlu: 5.938 ± 0.112	2.102GluPhe: 2.102 ± 0.049	4.639GluGly: 4.639 ± 0.078	1.452GluHis: 1.452 ± 0.036	3.872GluIle: 3.872 ± 0.07	4.491GluLys: 4.491 ± 0.077	7.21GluLeu: 7.21 ± 0.101	2.01GluMet: 2.01 ± 0.046	2.824GluAsn: 2.824 ± 0.055	2.215GluPro: 2.215 ± 0.052	3.48GluGln: 3.48 ± 0.08	4.021GluArg: 4.021 ± 0.082	2.894GluSer: 2.894 ± 0.052	3.445GluThr: 3.445 ± 0.066	4.14GluVal: 4.14 ± 0.072	0.622GluTrp: 0.622 ± 0.028	2.379GluTyr: 2.379 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	3.221PheAla: 3.221 ± 0.071	0.739PheCys: 0.739 ± 0.028	2.319PheAsp: 2.319 ± 0.053	2.07PheGlu: 2.07 ± 0.048	1.711PhePhe: 1.711 ± 0.05	3.034PheGly: 3.034 ± 0.061	0.856PheHis: 0.856 ± 0.031	1.924PheIle: 1.924 ± 0.053	1.266PheLys: 1.266 ± 0.04	3.985PheLeu: 3.985 ± 0.075	0.847PheMet: 0.847 ± 0.032	1.186PheAsn: 1.186 ± 0.036	1.552PhePro: 1.552 ± 0.041	1.567PheGln: 1.567 ± 0.04	1.857PheArg: 1.857 ± 0.048	2.734PheSer: 2.734 ± 0.06	2.339PheThr: 2.339 ± 0.054	2.596PheVal: 2.596 ± 0.052	0.488PheTrp: 0.488 ± 0.023	1.46PheTyr: 1.46 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	6.147GlyAla: 6.147 ± 0.096	1.38GlyCys: 1.38 ± 0.039	3.882GlyAsp: 3.882 ± 0.073	5.209GlyGlu: 5.209 ± 0.079	2.91GlyPhe: 2.91 ± 0.058	6.161GlyGly: 6.161 ± 0.093	1.367GlyHis: 1.367 ± 0.043	4.751GlyIle: 4.751 ± 0.088	4.649GlyLys: 4.649 ± 0.08	7.339GlyLeu: 7.339 ± 0.1	2.565GlyMet: 2.565 ± 0.058	2.63GlyAsn: 2.63 ± 0.061	2.03GlyPro: 2.03 ± 0.062	2.941GlyGln: 2.941 ± 0.061	3.994GlyArg: 3.994 ± 0.067	4.56GlySer: 4.56 ± 0.091	4.487GlyThr: 4.487 ± 0.075	6.187GlyVal: 6.187 ± 0.083	0.989GlyTrp: 0.989 ± 0.034	3.033GlyTyr: 3.033 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	1.313HisAla: 1.313 ± 0.038	0.371HisCys: 0.371 ± 0.019	0.917HisAsp: 0.917 ± 0.028	0.891HisGlu: 0.891 ± 0.032	0.808HisPhe: 0.808 ± 0.031	1.436HisGly: 1.436 ± 0.042	0.452HisHis: 0.452 ± 0.027	1.285HisIle: 1.285 ± 0.043	0.768HisLys: 0.768 ± 0.032	1.689HisLeu: 1.689 ± 0.046	0.507HisMet: 0.507 ± 0.022	0.609HisAsn: 0.609 ± 0.026	1.164HisPro: 1.164 ± 0.032	0.732HisGln: 0.732 ± 0.028	1.008HisArg: 1.008 ± 0.032	0.993HisSer: 0.993 ± 0.03	1.134HisThr: 1.134 ± 0.039	1.108HisVal: 1.108 ± 0.033	0.249HisTrp: 0.249 ± 0.017	0.72HisTyr: 0.72 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.091IleAla: 5.091 ± 0.078	1.052IleCys: 1.052 ± 0.032	3.101IleAsp: 3.101 ± 0.06	2.962IleGlu: 2.962 ± 0.067	2.202IlePhe: 2.202 ± 0.05	4.013IleGly: 4.013 ± 0.07	1.167IleHis: 1.167 ± 0.032	3.121IleIle: 3.121 ± 0.075	2.29IleLys: 2.29 ± 0.053	6.02IleLeu: 6.02 ± 0.091	1.305IleMet: 1.305 ± 0.043	1.958IleAsn: 1.958 ± 0.053	3.022IlePro: 3.022 ± 0.055	2.344IleGln: 2.344 ± 0.055	3.191IleArg: 3.191 ± 0.07	3.659IleSer: 3.659 ± 0.07	3.518IleThr: 3.518 ± 0.069	3.908IleVal: 3.908 ± 0.072	0.604IleTrp: 0.604 ± 0.025	1.915IleTyr: 1.915 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	4.671LysAla: 4.671 ± 0.082	0.533LysCys: 0.533 ± 0.025	2.781LysAsp: 2.781 ± 0.055	4.132LysGlu: 4.132 ± 0.083	1.317LysPhe: 1.317 ± 0.036	3.474LysGly: 3.474 ± 0.07	0.847LysHis: 0.847 ± 0.031	2.75LysIle: 2.75 ± 0.056	3.629LysLys: 3.629 ± 0.085	4.724LysLeu: 4.724 ± 0.068	1.417LysMet: 1.417 ± 0.04	1.947LysAsn: 1.947 ± 0.05	1.951LysPro: 1.951 ± 0.047	1.951LysGln: 1.951 ± 0.051	2.681LysArg: 2.681 ± 0.059	2.487LysSer: 2.487 ± 0.051	2.956LysThr: 2.956 ± 0.065	3.502LysVal: 3.502 ± 0.07	0.509LysTrp: 0.509 ± 0.022	1.733LysTyr: 1.733 ± 0.054	0.0LysXaa: 0.0 ± 0.0
Leu	9.595LeuAla: 9.595 ± 0.106	1.917LeuCys: 1.917 ± 0.045	5.867LeuAsp: 5.867 ± 0.083	7.353LeuGlu: 7.353 ± 0.102	3.989LeuPhe: 3.989 ± 0.084	7.341LeuGly: 7.341 ± 0.116	1.66LeuHis: 1.66 ± 0.042	5.068LeuIle: 5.068 ± 0.086	4.643LeuLys: 4.643 ± 0.062	10.582LeuLeu: 10.582 ± 0.151	2.792LeuMet: 2.792 ± 0.055	3.376LeuAsn: 3.376 ± 0.068	4.738LeuPro: 4.738 ± 0.081	3.035LeuGln: 3.035 ± 0.058	5.354LeuArg: 5.354 ± 0.085	7.165LeuSer: 7.165 ± 0.1	6.359LeuThr: 6.359 ± 0.09	6.905LeuVal: 6.905 ± 0.1	1.085LeuTrp: 1.085 ± 0.034	3.138LeuTyr: 3.138 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	2.842MetAla: 2.842 ± 0.061	0.316MetCys: 0.316 ± 0.019	1.91MetAsp: 1.91 ± 0.046	2.338MetGlu: 2.338 ± 0.052	0.848MetPhe: 0.848 ± 0.033	2.225MetGly: 2.225 ± 0.049	0.357MetHis: 0.357 ± 0.021	1.488MetIle: 1.488 ± 0.044	1.782MetLys: 1.782 ± 0.046	2.764MetLeu: 2.764 ± 0.056	0.788MetMet: 0.788 ± 0.031	1.18MetAsn: 1.18 ± 0.037	1.119MetPro: 1.119 ± 0.037	0.857MetGln: 0.857 ± 0.027	1.29MetArg: 1.29 ± 0.037	1.594MetSer: 1.594 ± 0.036	1.729MetThr: 1.729 ± 0.041	2.016MetVal: 2.016 ± 0.043	0.23MetTrp: 0.23 ± 0.016	0.601MetTyr: 0.601 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	2.895AsnAla: 2.895 ± 0.064	0.504AsnCys: 0.504 ± 0.024	1.623AsnAsp: 1.623 ± 0.048	1.833AsnGlu: 1.833 ± 0.05	1.269AsnPhe: 1.269 ± 0.039	2.954AsnGly: 2.954 ± 0.066	0.629AsnHis: 0.629 ± 0.028	2.166AsnIle: 2.166 ± 0.059	1.452AsnLys: 1.452 ± 0.043	3.421AsnLeu: 3.421 ± 0.062	0.934AsnMet: 0.934 ± 0.032	1.167AsnAsn: 1.167 ± 0.042	1.923AsnPro: 1.923 ± 0.047	1.315AsnGln: 1.315 ± 0.035	1.943AsnArg: 1.943 ± 0.046	1.764AsnSer: 1.764 ± 0.047	2.017AsnThr: 2.017 ± 0.057	2.261AsnVal: 2.261 ± 0.057	0.385AsnTrp: 0.385 ± 0.021	1.404AsnTyr: 1.404 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	3.651ProAla: 3.651 ± 0.072	0.547ProCys: 0.547 ± 0.025	2.776ProAsp: 2.776 ± 0.055	3.852ProGlu: 3.852 ± 0.074	1.689ProPhe: 1.689 ± 0.042	3.14ProGly: 3.14 ± 0.066	0.731ProHis: 0.731 ± 0.027	2.235ProIle: 2.235 ± 0.051	2.051ProLys: 2.051 ± 0.053	3.674ProLeu: 3.674 ± 0.071	1.199ProMet: 1.199 ± 0.034	1.353ProAsn: 1.353 ± 0.043	1.407ProPro: 1.407 ± 0.048	2.0ProGln: 2.0 ± 0.051	1.751ProArg: 1.751 ± 0.048	2.053ProSer: 2.053 ± 0.056	2.113ProThr: 2.113 ± 0.049	3.403ProVal: 3.403 ± 0.067	0.505ProTrp: 0.505 ± 0.027	1.455ProTyr: 1.455 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	4.226GlnAla: 4.226 ± 0.085	0.44GlnCys: 0.44 ± 0.022	1.97GlnAsp: 1.97 ± 0.045	3.319GlnGlu: 3.319 ± 0.066	1.252GlnPhe: 1.252 ± 0.038	2.956GlnGly: 2.956 ± 0.059	0.624GlnHis: 0.624 ± 0.026	2.219GlnIle: 2.219 ± 0.05	2.219GlnLys: 2.219 ± 0.059	4.052GlnLeu: 4.052 ± 0.066	1.286GlnMet: 1.286 ± 0.039	1.512GlnAsn: 1.512 ± 0.047	1.543GlnPro: 1.543 ± 0.039	1.843GlnGln: 1.843 ± 0.053	2.353GlnArg: 2.353 ± 0.055	2.093GlnSer: 2.093 ± 0.048	2.126GlnThr: 2.126 ± 0.048	3.081GlnVal: 3.081 ± 0.063	0.491GlnTrp: 0.491 ± 0.025	1.386GlnTyr: 1.386 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	4.186ArgAla: 4.186 ± 0.071	0.847ArgCys: 0.847 ± 0.03	2.823ArgAsp: 2.823 ± 0.064	4.392ArgGlu: 4.392 ± 0.081	2.085ArgPhe: 2.085 ± 0.052	3.305ArgGly: 3.305 ± 0.065	0.985ArgHis: 0.985 ± 0.032	2.843ArgIle: 2.843 ± 0.058	2.937ArgLys: 2.937 ± 0.06	5.413ArgLeu: 5.413 ± 0.095	1.679ArgMet: 1.679 ± 0.043	1.712ArgAsn: 1.712 ± 0.045	2.183ArgPro: 2.183 ± 0.045	2.556ArgGln: 2.556 ± 0.051	3.798ArgArg: 3.798 ± 0.094	2.849ArgSer: 2.849 ± 0.055	2.827ArgThr: 2.827 ± 0.059	3.275ArgVal: 3.275 ± 0.07	0.692ArgTrp: 0.692 ± 0.029	1.995ArgTyr: 1.995 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	4.967SerAla: 4.967 ± 0.085	0.848SerCys: 0.848 ± 0.031	3.048SerAsp: 3.048 ± 0.068	2.947SerGlu: 2.947 ± 0.061	2.365SerPhe: 2.365 ± 0.049	5.2SerGly: 5.2 ± 0.099	1.105SerHis: 1.105 ± 0.032	3.302SerIle: 3.302 ± 0.068	2.397SerLys: 2.397 ± 0.062	5.338SerLeu: 5.338 ± 0.086	1.617SerMet: 1.617 ± 0.041	1.819SerAsn: 1.819 ± 0.052	2.288SerPro: 2.288 ± 0.051	2.513SerGln: 2.513 ± 0.055	3.091SerArg: 3.091 ± 0.058	3.419SerSer: 3.419 ± 0.107	2.949SerThr: 2.949 ± 0.057	4.082SerVal: 4.082 ± 0.077	0.662SerTrp: 0.662 ± 0.027	2.053SerTyr: 2.053 ± 0.058	0.0SerXaa: 0.0 ± 0.0
Thr	5.582ThrAla: 5.582 ± 0.095	0.742ThrCys: 0.742 ± 0.03	3.013ThrAsp: 3.013 ± 0.064	2.967ThrGlu: 2.967 ± 0.058	2.11ThrPhe: 2.11 ± 0.049	4.949ThrGly: 4.949 ± 0.083	0.99ThrHis: 0.99 ± 0.032	3.402ThrIle: 3.402 ± 0.066	2.244ThrLys: 2.244 ± 0.057	5.916ThrLeu: 5.916 ± 0.09	1.437ThrMet: 1.437 ± 0.043	1.665ThrAsn: 1.665 ± 0.048	2.875ThrPro: 2.875 ± 0.057	2.228ThrGln: 2.228 ± 0.048	2.522ThrArg: 2.522 ± 0.052	2.766ThrSer: 2.766 ± 0.069	2.978ThrThr: 2.978 ± 0.071	4.983ThrVal: 4.983 ± 0.083	0.614ThrTrp: 0.614 ± 0.025	1.844ThrTyr: 1.844 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	6.073ValAla: 6.073 ± 0.092	1.392ValCys: 1.392 ± 0.043	4.168ValAsp: 4.168 ± 0.074	4.736ValGlu: 4.736 ± 0.081	2.822ValPhe: 2.822 ± 0.061	5.107ValGly: 5.107 ± 0.077	1.199ValHis: 1.199 ± 0.035	4.18ValIle: 4.18 ± 0.074	3.41ValLys: 3.41 ± 0.07	7.839ValLeu: 7.839 ± 0.096	2.047ValMet: 2.047 ± 0.048	2.41ValAsn: 2.41 ± 0.054	3.049ValPro: 3.049 ± 0.057	2.281ValGln: 2.281 ± 0.046	3.613ValArg: 3.613 ± 0.082	4.553ValSer: 4.553 ± 0.077	4.442ValThr: 4.442 ± 0.078	5.431ValVal: 5.431 ± 0.092	0.833ValTrp: 0.833 ± 0.031	2.443ValTyr: 2.443 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.925TrpAla: 0.925 ± 0.034	0.191TrpCys: 0.191 ± 0.017	0.688TrpAsp: 0.688 ± 0.028	0.799TrpGlu: 0.799 ± 0.034	0.467TrpPhe: 0.467 ± 0.022	0.846TrpGly: 0.846 ± 0.034	0.178TrpHis: 0.178 ± 0.014	0.529TrpIle: 0.529 ± 0.024	0.581TrpLys: 0.581 ± 0.026	1.273TrpLeu: 1.273 ± 0.046	0.37TrpMet: 0.37 ± 0.021	0.516TrpAsn: 0.516 ± 0.025	0.332TrpPro: 0.332 ± 0.018	0.557TrpGln: 0.557 ± 0.023	0.611TrpArg: 0.611 ± 0.026	0.654TrpSer: 0.654 ± 0.027	0.523TrpThr: 0.523 ± 0.023	0.695TrpVal: 0.695 ± 0.028	0.148TrpTrp: 0.148 ± 0.014	0.416TrpTyr: 0.416 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.82TyrAla: 2.82 ± 0.058	0.604TyrCys: 0.604 ± 0.025	2.297TyrAsp: 2.297 ± 0.058	2.206TyrGlu: 2.206 ± 0.052	1.486TyrPhe: 1.486 ± 0.042	2.754TyrGly: 2.754 ± 0.052	0.741TyrHis: 0.741 ± 0.031	1.957TyrIle: 1.957 ± 0.051	1.301TyrLys: 1.301 ± 0.041	3.601TyrLeu: 3.601 ± 0.065	0.737TyrMet: 0.737 ± 0.031	1.325TyrAsn: 1.325 ± 0.047	1.406TyrPro: 1.406 ± 0.041	1.563TyrGln: 1.563 ± 0.038	2.031TyrArg: 2.031 ± 0.049	1.929TyrSer: 1.929 ± 0.051	2.186TyrThr: 2.186 ± 0.052	2.206TyrVal: 2.206 ± 0.056	0.376TyrTrp: 0.376 ± 0.023	1.485TyrTyr: 1.485 ± 0.045	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2987 proteins (924795 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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