Plesiocystis pacifica SIR-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Myxococcales; Nannocystineae; Nannocystaceae; Plesiocystis; Plesiocystis pacifica

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8437 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6GBS3|A6GBS3_9DELT Uncharacterized protein OS=Plesiocystis pacifica SIR-1 OX=391625 GN=PPSIR1_38219 PE=4 SV=1
MM1 pKa = 7.52SSPARR6 pKa = 11.84SLALTFTASLACLALSLFACADD28 pKa = 4.05DD29 pKa = 5.53DD30 pKa = 5.02PPLGDD35 pKa = 4.87PPPNSPGGGGTLTAGGGDD53 pKa = 3.46GGGGGTLTAGGGGDD67 pKa = 4.18DD68 pKa = 4.93EE69 pKa = 5.15VGEE72 pKa = 5.17DD73 pKa = 5.0DD74 pKa = 4.1PCSDD78 pKa = 4.44LGSTAVDD85 pKa = 5.48FSYY88 pKa = 10.85LWVANTDD95 pKa = 3.38EE96 pKa = 4.95GSVSKK101 pKa = 11.16VNAKK105 pKa = 8.03TQVEE109 pKa = 4.44VARR112 pKa = 11.84YY113 pKa = 6.51RR114 pKa = 11.84TGPANVEE121 pKa = 4.1HH122 pKa = 6.65SPSRR126 pKa = 11.84TTVSLDD132 pKa = 2.8GRR134 pKa = 11.84YY135 pKa = 10.08ALVGNRR141 pKa = 11.84LSGSVVLVAAEE152 pKa = 4.15TGDD155 pKa = 3.98CVDD158 pKa = 3.98ANEE161 pKa = 5.37DD162 pKa = 3.74GLITTSQSPDD172 pKa = 3.78DD173 pKa = 4.24LLDD176 pKa = 3.67WGDD179 pKa = 4.19DD180 pKa = 3.7EE181 pKa = 5.54CVLWSTNMPQIGEE194 pKa = 4.52GISAGPRR201 pKa = 11.84GMTFDD206 pKa = 4.36PGEE209 pKa = 5.36LDD211 pKa = 3.64TQTCTYY217 pKa = 10.09QDD219 pKa = 3.06AKK221 pKa = 10.73IWVGWGGASTDD232 pKa = 3.53LAHH235 pKa = 7.78IGRR238 pKa = 11.84LDD240 pKa = 3.67PATGTFEE247 pKa = 4.14EE248 pKa = 5.38LIAVEE253 pKa = 4.73DD254 pKa = 3.94WGSSLGPVHH263 pKa = 6.94APYY266 pKa = 10.54GAAHH270 pKa = 7.13DD271 pKa = 4.8GDD273 pKa = 3.82HH274 pKa = 6.5SVWFTGLRR282 pKa = 11.84GEE284 pKa = 4.4YY285 pKa = 10.46LRR287 pKa = 11.84IDD289 pKa = 3.93TEE291 pKa = 4.3DD292 pKa = 4.57LDD294 pKa = 3.88VDD296 pKa = 3.75RR297 pKa = 11.84WIAPGWAAPYY307 pKa = 10.74GMAVDD312 pKa = 3.6AEE314 pKa = 4.36GRR316 pKa = 11.84VWTGGCFGPVSVFDD330 pKa = 4.83PDD332 pKa = 3.93TEE334 pKa = 4.23IVTEE338 pKa = 4.09IVGTDD343 pKa = 2.83ACHH346 pKa = 6.72RR347 pKa = 11.84GVAADD352 pKa = 3.46TKK354 pKa = 9.81GNVWVAMNEE363 pKa = 3.96PCGLVQIDD371 pKa = 3.81RR372 pKa = 11.84DD373 pKa = 3.86DD374 pKa = 4.28EE375 pKa = 4.39EE376 pKa = 4.74VVNVLTAEE384 pKa = 4.58DD385 pKa = 4.44FDD387 pKa = 4.71GLCSIPVGVSVDD399 pKa = 3.23ADD401 pKa = 3.69DD402 pKa = 4.3FVWMVDD408 pKa = 3.3QLGWAWRR415 pKa = 11.84IDD417 pKa = 3.96PEE419 pKa = 4.51SLDD422 pKa = 3.48MDD424 pKa = 4.17YY425 pKa = 11.79LEE427 pKa = 5.42IPGNHH432 pKa = 5.1YY433 pKa = 9.77TYY435 pKa = 11.62SDD437 pKa = 3.46MTGGGLNAVVYY448 pKa = 8.07PQQ450 pKa = 3.24

Molecular weight: 47.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6GEB9|A6GEB9_9DELT (2Fe-2S)-binding protein OS=Plesiocystis pacifica SIR-1 OX=391625 GN=PPSIR1_17675 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84APSTASRR11 pKa = 11.84RR12 pKa = 11.84AGVHH16 pKa = 5.25SSAMAAPAPAQGSTGAAGSAPPTRR40 pKa = 11.84AKK42 pKa = 10.5LSSAPAPAVPTPMAASHH59 pKa = 5.88RR60 pKa = 11.84RR61 pKa = 11.84LRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84ARR67 pKa = 11.84GSARR71 pKa = 11.84RR72 pKa = 11.84AASSSAMVGKK82 pKa = 10.47RR83 pKa = 11.84SSTSTSRR90 pKa = 11.84ARR92 pKa = 11.84SRR94 pKa = 11.84ATSTWLGTFASRR106 pKa = 11.84FVSLLASLGGARR118 pKa = 11.84RR119 pKa = 11.84WPSRR123 pKa = 11.84TAMAA127 pKa = 5.11

Molecular weight: 13.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8437

0

8437

3147198

17

4933

373.0

40.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.675 ± 0.037

1.225 ± 0.015

6.439 ± 0.024

7.553 ± 0.026

3.082 ± 0.016

9.064 ± 0.029

2.109 ± 0.013

3.391 ± 0.019

2.303 ± 0.023

10.505 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.845 ± 0.011

1.941 ± 0.013

5.868 ± 0.021

2.918 ± 0.013

7.698 ± 0.036

5.594 ± 0.02

4.952 ± 0.023

7.32 ± 0.02

1.537 ± 0.01

1.983 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski