Amino acid dipepetide frequency for Claviceps purpurea (strain 20.1) (Ergot fungus) (Sphacelia segetum)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.992AlaAla: 9.992 ± 0.091	1.094AlaCys: 1.094 ± 0.018	4.617AlaAsp: 4.617 ± 0.032	5.344AlaGlu: 5.344 ± 0.058	3.046AlaPhe: 3.046 ± 0.026	5.887AlaGly: 5.887 ± 0.048	1.992AlaHis: 1.992 ± 0.024	4.079AlaIle: 4.079 ± 0.034	4.216AlaLys: 4.216 ± 0.037	7.817AlaLeu: 7.817 ± 0.047	2.136AlaMet: 2.136 ± 0.027	2.92AlaAsn: 2.92 ± 0.026	4.9AlaPro: 4.9 ± 0.051	3.647AlaGln: 3.647 ± 0.042	5.377AlaArg: 5.377 ± 0.045	7.92AlaSer: 7.92 ± 0.058	5.334AlaThr: 5.334 ± 0.039	5.453AlaVal: 5.453 ± 0.043	1.167AlaTrp: 1.167 ± 0.018	2.052AlaTyr: 2.052 ± 0.024	0.0AlaXaa: 0.0 ± 0.0
Cys	0.991CysAla: 0.991 ± 0.017	0.256CysCys: 0.256 ± 0.009	0.746CysAsp: 0.746 ± 0.016	0.658CysGlu: 0.658 ± 0.012	0.546CysPhe: 0.546 ± 0.012	0.916CysGly: 0.916 ± 0.017	0.397CysHis: 0.397 ± 0.01	0.672CysIle: 0.672 ± 0.014	0.587CysLys: 0.587 ± 0.012	1.304CysLeu: 1.304 ± 0.02	0.265CysMet: 0.265 ± 0.008	0.418CysAsn: 0.418 ± 0.011	0.717CysPro: 0.717 ± 0.015	0.487CysGln: 0.487 ± 0.011	0.823CysArg: 0.823 ± 0.013	0.952CysSer: 0.952 ± 0.016	0.702CysThr: 0.702 ± 0.014	0.794CysVal: 0.794 ± 0.014	0.185CysTrp: 0.185 ± 0.007	0.357CysTyr: 0.357 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	5.362AspAla: 5.362 ± 0.038	0.716AspCys: 0.716 ± 0.015	5.069AspAsp: 5.069 ± 0.052	4.893AspGlu: 4.893 ± 0.049	2.278AspPhe: 2.278 ± 0.027	4.507AspGly: 4.507 ± 0.034	1.344AspHis: 1.344 ± 0.019	2.956AspIle: 2.956 ± 0.026	2.669AspLys: 2.669 ± 0.026	5.173AspLeu: 5.173 ± 0.039	1.494AspMet: 1.494 ± 0.018	1.879AspAsn: 1.879 ± 0.023	3.063AspPro: 3.063 ± 0.026	2.001AspGln: 2.001 ± 0.026	3.244AspArg: 3.244 ± 0.033	4.274AspSer: 4.274 ± 0.037	2.965AspThr: 2.965 ± 0.029	3.999AspVal: 3.999 ± 0.032	0.903AspTrp: 0.903 ± 0.015	1.53AspTyr: 1.53 ± 0.019	0.0AspXaa: 0.0 ± 0.0
Glu	5.859GluAla: 5.859 ± 0.055	0.652GluCys: 0.652 ± 0.014	4.386GluAsp: 4.386 ± 0.037	5.454GluGlu: 5.454 ± 0.064	1.903GluPhe: 1.903 ± 0.021	3.682GluGly: 3.682 ± 0.032	1.486GluHis: 1.486 ± 0.019	2.843GluIle: 2.843 ± 0.028	3.559GluLys: 3.559 ± 0.04	5.261GluLeu: 5.261 ± 0.039	1.529GluMet: 1.529 ± 0.021	2.156GluAsn: 2.156 ± 0.024	2.799GluPro: 2.799 ± 0.037	2.629GluGln: 2.629 ± 0.029	4.01GluArg: 4.01 ± 0.046	4.298GluSer: 4.298 ± 0.037	3.448GluThr: 3.448 ± 0.03	3.482GluVal: 3.482 ± 0.036	0.889GluTrp: 0.889 ± 0.016	1.652GluTyr: 1.652 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	2.925PheAla: 2.925 ± 0.029	0.543PheCys: 0.543 ± 0.011	2.332PheAsp: 2.332 ± 0.024	2.136PheGlu: 2.136 ± 0.023	1.452PhePhe: 1.452 ± 0.024	2.619PheGly: 2.619 ± 0.034	0.874PheHis: 0.874 ± 0.016	1.603PheIle: 1.603 ± 0.023	1.453PheLys: 1.453 ± 0.022	3.345PheLeu: 3.345 ± 0.035	0.78PheMet: 0.78 ± 0.011	1.275PheAsn: 1.275 ± 0.019	1.908PhePro: 1.908 ± 0.024	1.325PheGln: 1.325 ± 0.019	2.031PheArg: 2.031 ± 0.025	2.816PheSer: 2.816 ± 0.024	1.948PheThr: 1.948 ± 0.026	2.349PheVal: 2.349 ± 0.027	0.584PheTrp: 0.584 ± 0.014	0.978PheTyr: 0.978 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	5.376GlyAla: 5.376 ± 0.05	0.89GlyCys: 0.89 ± 0.016	3.861GlyAsp: 3.861 ± 0.035	3.695GlyGlu: 3.695 ± 0.032	2.568GlyPhe: 2.568 ± 0.027	5.945GlyGly: 5.945 ± 0.079	1.816GlyHis: 1.816 ± 0.024	3.256GlyIle: 3.256 ± 0.033	3.409GlyLys: 3.409 ± 0.03	5.742GlyLeu: 5.742 ± 0.041	1.592GlyMet: 1.592 ± 0.024	2.482GlyAsn: 2.482 ± 0.036	3.411GlyPro: 3.411 ± 0.035	2.654GlyGln: 2.654 ± 0.029	4.451GlyArg: 4.451 ± 0.034	5.749GlySer: 5.749 ± 0.05	3.83GlyThr: 3.83 ± 0.044	4.116GlyVal: 4.116 ± 0.036	1.063GlyTrp: 1.063 ± 0.019	1.896GlyTyr: 1.896 ± 0.024	0.0GlyXaa: 0.0 ± 0.0
His	2.061HisAla: 2.061 ± 0.023	0.36HisCys: 0.36 ± 0.01	1.582HisAsp: 1.582 ± 0.019	1.494HisGlu: 1.494 ± 0.02	0.956HisPhe: 0.956 ± 0.014	1.943HisGly: 1.943 ± 0.02	1.05HisHis: 1.05 ± 0.023	1.232HisIle: 1.232 ± 0.019	1.002HisLys: 1.002 ± 0.015	2.367HisLeu: 2.367 ± 0.028	0.546HisMet: 0.546 ± 0.012	0.874HisAsn: 0.874 ± 0.014	1.621HisPro: 1.621 ± 0.024	1.169HisGln: 1.169 ± 0.026	1.654HisArg: 1.654 ± 0.023	1.96HisSer: 1.96 ± 0.025	1.283HisThr: 1.283 ± 0.021	1.655HisVal: 1.655 ± 0.02	0.336HisTrp: 0.336 ± 0.009	0.675HisTyr: 0.675 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	3.912IleAla: 3.912 ± 0.032	0.716IleCys: 0.716 ± 0.014	2.804IleAsp: 2.804 ± 0.026	2.697IleGlu: 2.697 ± 0.027	1.825IlePhe: 1.825 ± 0.026	2.858IleGly: 2.858 ± 0.032	1.26IleHis: 1.26 ± 0.017	2.157IleIle: 2.157 ± 0.027	2.14IleLys: 2.14 ± 0.024	4.227IleLeu: 4.227 ± 0.037	1.033IleMet: 1.033 ± 0.017	1.603IleAsn: 1.603 ± 0.02	2.805IlePro: 2.805 ± 0.03	1.832IleGln: 1.832 ± 0.022	2.813IleArg: 2.813 ± 0.024	3.553IleSer: 3.553 ± 0.031	2.578IleThr: 2.578 ± 0.03	2.972IleVal: 2.972 ± 0.032	0.7IleTrp: 0.7 ± 0.013	1.197IleTyr: 1.197 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	4.432LysAla: 4.432 ± 0.037	0.519LysCys: 0.519 ± 0.011	2.863LysAsp: 2.863 ± 0.029	3.209LysGlu: 3.209 ± 0.037	1.468LysPhe: 1.468 ± 0.023	2.88LysGly: 2.88 ± 0.032	1.186LysHis: 1.186 ± 0.018	2.285LysIle: 2.285 ± 0.023	3.417LysLys: 3.417 ± 0.048	4.151LysLeu: 4.151 ± 0.037	1.124LysMet: 1.124 ± 0.018	1.747LysAsn: 1.747 ± 0.023	2.621LysPro: 2.621 ± 0.029	1.928LysGln: 1.928 ± 0.025	3.484LysArg: 3.484 ± 0.039	3.503LysSer: 3.503 ± 0.035	2.953LysThr: 2.953 ± 0.027	2.775LysVal: 2.775 ± 0.027	0.67LysTrp: 0.67 ± 0.014	1.319LysTyr: 1.319 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	7.871LeuAla: 7.871 ± 0.053	1.248LeuCys: 1.248 ± 0.018	5.433LeuAsp: 5.433 ± 0.039	5.549LeuGlu: 5.549 ± 0.043	3.14LeuPhe: 3.14 ± 0.034	5.744LeuGly: 5.744 ± 0.039	2.291LeuHis: 2.291 ± 0.024	3.571LeuIle: 3.571 ± 0.036	4.138LeuLys: 4.138 ± 0.035	8.097LeuLeu: 8.097 ± 0.068	1.856LeuMet: 1.856 ± 0.021	2.877LeuAsn: 2.877 ± 0.027	5.464LeuPro: 5.464 ± 0.042	3.91LeuGln: 3.91 ± 0.036	6.178LeuArg: 6.178 ± 0.047	7.259LeuSer: 7.259 ± 0.051	4.646LeuThr: 4.646 ± 0.036	5.283LeuVal: 5.283 ± 0.04	1.148LeuTrp: 1.148 ± 0.017	2.253LeuTyr: 2.253 ± 0.024	0.0LeuXaa: 0.0 ± 0.0
Met	2.446MetAla: 2.446 ± 0.024	0.263MetCys: 0.263 ± 0.009	1.397MetAsp: 1.397 ± 0.02	1.375MetGlu: 1.375 ± 0.017	0.715MetPhe: 0.715 ± 0.015	1.484MetGly: 1.484 ± 0.021	0.515MetHis: 0.515 ± 0.011	0.96MetIle: 0.96 ± 0.016	1.103MetLys: 1.103 ± 0.014	1.926MetLeu: 1.926 ± 0.022	0.626MetMet: 0.626 ± 0.013	0.819MetAsn: 0.819 ± 0.017	1.375MetPro: 1.375 ± 0.018	0.939MetGln: 0.939 ± 0.017	1.427MetArg: 1.427 ± 0.02	2.036MetSer: 2.036 ± 0.025	1.394MetThr: 1.394 ± 0.017	1.3MetVal: 1.3 ± 0.02	0.271MetTrp: 0.271 ± 0.008	0.549MetTyr: 0.549 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.072AsnAla: 3.072 ± 0.028	0.459AsnCys: 0.459 ± 0.011	2.091AsnAsp: 2.091 ± 0.022	1.941AsnGlu: 1.941 ± 0.021	1.252AsnPhe: 1.252 ± 0.019	2.757AsnGly: 2.757 ± 0.038	0.845AsnHis: 0.845 ± 0.018	1.738AsnIle: 1.738 ± 0.02	1.62AsnLys: 1.62 ± 0.022	3.095AsnLeu: 3.095 ± 0.029	0.91AsnMet: 0.91 ± 0.017	1.414AsnAsn: 1.414 ± 0.031	2.184AsnPro: 2.184 ± 0.025	1.229AsnGln: 1.229 ± 0.021	1.952AsnArg: 1.952 ± 0.028	2.599AsnSer: 2.599 ± 0.027	1.994AsnThr: 1.994 ± 0.024	2.209AsnVal: 2.209 ± 0.027	0.482AsnTrp: 0.482 ± 0.011	0.956AsnTyr: 0.956 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	5.197ProAla: 5.197 ± 0.05	0.614ProCys: 0.614 ± 0.013	3.264ProAsp: 3.264 ± 0.033	3.572ProGlu: 3.572 ± 0.028	1.909ProPhe: 1.909 ± 0.024	3.807ProGly: 3.807 ± 0.035	1.442ProHis: 1.442 ± 0.022	2.389ProIle: 2.389 ± 0.024	2.525ProLys: 2.525 ± 0.028	4.783ProLeu: 4.783 ± 0.036	1.153ProMet: 1.153 ± 0.017	2.076ProAsn: 2.076 ± 0.023	5.205ProPro: 5.205 ± 0.078	2.497ProGln: 2.497 ± 0.033	3.607ProArg: 3.607 ± 0.032	6.062ProSer: 6.062 ± 0.05	3.721ProThr: 3.721 ± 0.036	3.485ProVal: 3.485 ± 0.034	0.742ProTrp: 0.742 ± 0.013	1.33ProTyr: 1.33 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	3.606GlnAla: 3.606 ± 0.035	0.444GlnCys: 0.444 ± 0.012	2.398GlnAsp: 2.398 ± 0.026	2.5GlnGlu: 2.5 ± 0.032	1.26GlnPhe: 1.26 ± 0.017	2.478GlnGly: 2.478 ± 0.028	1.311GlnHis: 1.311 ± 0.025	1.858GlnIle: 1.858 ± 0.024	2.012GlnLys: 2.012 ± 0.027	3.594GlnLeu: 3.594 ± 0.035	0.952GlnMet: 0.952 ± 0.016	1.497GlnAsn: 1.497 ± 0.022	2.558GlnPro: 2.558 ± 0.032	3.279GlnGln: 3.279 ± 0.114	2.805GlnArg: 2.805 ± 0.03	3.287GlnSer: 3.287 ± 0.031	2.332GlnThr: 2.332 ± 0.024	2.189GlnVal: 2.189 ± 0.02	0.581GlnTrp: 0.581 ± 0.011	1.112GlnTyr: 1.112 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	5.059ArgAla: 5.059 ± 0.039	0.84ArgCys: 0.84 ± 0.014	3.846ArgAsp: 3.846 ± 0.042	4.016ArgGlu: 4.016 ± 0.041	2.233ArgPhe: 2.233 ± 0.026	4.129ArgGly: 4.129 ± 0.039	1.829ArgHis: 1.829 ± 0.021	2.849ArgIle: 2.849 ± 0.03	3.536ArgLys: 3.536 ± 0.033	5.691ArgLeu: 5.691 ± 0.04	1.392ArgMet: 1.392 ± 0.019	2.302ArgAsn: 2.302 ± 0.031	3.699ArgPro: 3.699 ± 0.038	2.944ArgGln: 2.944 ± 0.033	5.601ArgArg: 5.601 ± 0.051	4.997ArgSer: 4.997 ± 0.045	3.406ArgThr: 3.406 ± 0.029	3.473ArgVal: 3.473 ± 0.033	0.905ArgTrp: 0.905 ± 0.015	1.658ArgTyr: 1.658 ± 0.02	0.0ArgXaa: 0.0 ± 0.0
Ser	6.967SerAla: 6.967 ± 0.052	0.959SerCys: 0.959 ± 0.017	4.455SerAsp: 4.455 ± 0.041	4.176SerGlu: 4.176 ± 0.04	2.867SerPhe: 2.867 ± 0.031	5.529SerGly: 5.529 ± 0.045	2.158SerHis: 2.158 ± 0.023	3.874SerIle: 3.874 ± 0.038	3.725SerLys: 3.725 ± 0.039	7.161SerLeu: 7.161 ± 0.053	1.855SerMet: 1.855 ± 0.022	2.838SerAsn: 2.838 ± 0.029	5.501SerPro: 5.501 ± 0.055	3.393SerGln: 3.393 ± 0.034	5.453SerArg: 5.453 ± 0.046	9.414SerSer: 9.414 ± 0.091	5.511SerThr: 5.511 ± 0.05	4.572SerVal: 4.572 ± 0.035	1.059SerTrp: 1.059 ± 0.015	1.945SerTyr: 1.945 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	5.3ThrAla: 5.3 ± 0.045	0.753ThrCys: 0.753 ± 0.013	2.857ThrAsp: 2.857 ± 0.027	3.048ThrGlu: 3.048 ± 0.036	2.033ThrPhe: 2.033 ± 0.024	4.04ThrGly: 4.04 ± 0.039	1.349ThrHis: 1.349 ± 0.022	2.845ThrIle: 2.845 ± 0.031	2.668ThrLys: 2.668 ± 0.03	5.057ThrLeu: 5.057 ± 0.039	1.275ThrMet: 1.275 ± 0.017	1.974ThrAsn: 1.974 ± 0.023	4.083ThrPro: 4.083 ± 0.038	2.137ThrGln: 2.137 ± 0.025	3.327ThrArg: 3.327 ± 0.031	5.316ThrSer: 5.316 ± 0.043	4.258ThrThr: 4.258 ± 0.054	3.484ThrVal: 3.484 ± 0.036	0.803ThrTrp: 0.803 ± 0.013	1.442ThrTyr: 1.442 ± 0.021	0.0ThrXaa: 0.0 ± 0.0
Val	5.402ValAla: 5.402 ± 0.037	0.81ValCys: 0.81 ± 0.012	3.773ValAsp: 3.773 ± 0.033	3.891ValGlu: 3.891 ± 0.036	2.301ValPhe: 2.301 ± 0.029	3.821ValGly: 3.821 ± 0.033	1.513ValHis: 1.513 ± 0.019	2.622ValIle: 2.622 ± 0.029	2.896ValLys: 2.896 ± 0.029	5.483ValLeu: 5.483 ± 0.047	1.393ValMet: 1.393 ± 0.017	2.07ValAsn: 2.07 ± 0.024	3.57ValPro: 3.57 ± 0.034	2.436ValGln: 2.436 ± 0.026	3.626ValArg: 3.626 ± 0.031	4.583ValSer: 4.583 ± 0.042	3.376ValThr: 3.376 ± 0.035	4.277ValVal: 4.277 ± 0.037	0.833ValTrp: 0.833 ± 0.015	1.554ValTyr: 1.554 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	1.139TrpAla: 1.139 ± 0.018	0.221TrpCys: 0.221 ± 0.008	0.891TrpAsp: 0.891 ± 0.015	0.829TrpGlu: 0.829 ± 0.015	0.46TrpPhe: 0.46 ± 0.012	0.826TrpGly: 0.826 ± 0.017	0.375TrpHis: 0.375 ± 0.008	0.688TrpIle: 0.688 ± 0.012	0.775TrpLys: 0.775 ± 0.014	1.281TrpLeu: 1.281 ± 0.017	0.373TrpMet: 0.373 ± 0.009	0.59TrpAsn: 0.59 ± 0.013	0.637TrpPro: 0.637 ± 0.012	0.591TrpGln: 0.591 ± 0.01	0.941TrpArg: 0.941 ± 0.015	0.984TrpSer: 0.984 ± 0.016	0.913TrpThr: 0.913 ± 0.015	0.805TrpVal: 0.805 ± 0.015	0.29TrpTrp: 0.29 ± 0.01	0.381TrpTyr: 0.381 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.019TyrAla: 2.019 ± 0.025	0.417TyrCys: 0.417 ± 0.008	1.653TyrAsp: 1.653 ± 0.024	1.5TyrGlu: 1.5 ± 0.021	1.064TyrPhe: 1.064 ± 0.016	1.949TyrGly: 1.949 ± 0.028	0.787TyrHis: 0.787 ± 0.013	1.206TyrIle: 1.206 ± 0.016	1.087TyrLys: 1.087 ± 0.017	2.403TyrLeu: 2.403 ± 0.026	0.637TyrMet: 0.637 ± 0.012	0.987TyrAsn: 0.987 ± 0.017	1.26TyrPro: 1.26 ± 0.018	1.024TyrGln: 1.024 ± 0.016	1.604TyrArg: 1.604 ± 0.021	1.848TyrSer: 1.848 ± 0.023	1.456TyrThr: 1.456 ± 0.02	1.559TyrVal: 1.559 ± 0.022	0.377TyrTrp: 0.377 ± 0.011	0.799TyrTyr: 0.799 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8814 proteins (4252061 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---