Paeniclostridium sordellii (Clostridium sordellii)
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3465 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A1SH11|A0A0A1SH11_PAESO bPH_1 domain-containing protein OS=Paeniclostridium sordellii OX=1505 GN=JGS6382_14181 PE=4 SV=1
MM1 pKa = 7.64 SKK3 pKa = 10.57 LDD5 pKa = 3.38 KK6 pKa = 10.57 LLRR9 pKa = 11.84 SFGCGCNSPFGGSSLLIIAVVVFLLLGTDD38 pKa = 3.43 LLDD41 pKa = 5.66 NFFCDD46 pKa = 3.83 DD47 pKa = 4.64 NSWIWIILIILLLFNFDD64 pKa = 5.28 DD65 pKa = 5.19 GCCC68 pKa = 3.38
Molecular weight: 7.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.884
IPC_protein 3.77
Toseland 3.554
ProMoST 4.024
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.872
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.973
DTASelect 4.291
Thurlkill 3.668
EMBOSS 3.859
Sillero 3.91
Patrickios 0.604
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.812
Protein with the highest isoelectric point:
>tr|A0A0A1SFU3|A0A0A1SFU3_PAESO UPF0597 protein JGS6382_01961 OS=Paeniclostridium sordellii OX=1505 GN=JGS6382_01961 PE=3 SV=1
MM1 pKa = 7.35 ARR3 pKa = 11.84 KK4 pKa = 10.09 AMVVKK9 pKa = 9.84 QQKK12 pKa = 7.33 KK13 pKa = 6.89 QKK15 pKa = 9.04 YY16 pKa = 5.95 ATRR19 pKa = 11.84 EE20 pKa = 3.86 YY21 pKa = 10.06 TRR23 pKa = 11.84 CTICGRR29 pKa = 11.84 PHH31 pKa = 5.69 SVLRR35 pKa = 11.84 KK36 pKa = 9.55 FGICRR41 pKa = 11.84 ICFRR45 pKa = 11.84 EE46 pKa = 3.97 LAYY49 pKa = 9.84 KK50 pKa = 10.14 GQIPGVRR57 pKa = 11.84 KK58 pKa = 10.13 ASWW61 pKa = 2.78
Molecular weight: 7.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.882
IPC_protein 10.511
Toseland 10.833
ProMoST 10.452
Dawson 10.921
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.301
Grimsley 10.965
Solomon 11.008
Lehninger 10.994
Nozaki 10.833
DTASelect 10.584
Thurlkill 10.818
EMBOSS 11.228
Sillero 10.847
Patrickios 11.052
IPC_peptide 11.023
IPC2_peptide 9.75
IPC2.peptide.svr19 8.385
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3465
0
3465
1022320
20
2618
295.0
33.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.067 ± 0.044
1.185 ± 0.02
5.779 ± 0.035
7.145 ± 0.049
4.368 ± 0.036
6.178 ± 0.039
1.315 ± 0.016
10.571 ± 0.056
9.591 ± 0.047
9.061 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.636 ± 0.021
6.823 ± 0.047
2.634 ± 0.021
2.239 ± 0.019
2.95 ± 0.026
6.456 ± 0.04
4.784 ± 0.028
6.41 ± 0.037
0.584 ± 0.011
4.224 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here