[Candida] arabinofermentans NRRL YB-2248
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5827 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E4SYK6|A0A1E4SYK6_9ASCO Sm domain-containing protein OS=[Candida] arabinofermentans NRRL YB-2248 OX=983967 GN=CANARDRAFT_200819 PE=3 SV=1
MM1 pKa = 7.14 NRR3 pKa = 11.84 STSTSEE9 pKa = 3.63 IQSISASSIITDD21 pKa = 3.76 TDD23 pKa = 3.01 EE24 pKa = 4.18 SAYY27 pKa = 9.65 FTDD30 pKa = 4.32 ADD32 pKa = 3.88 SHH34 pKa = 5.78 FTDD37 pKa = 5.12 TEE39 pKa = 4.44 LNDD42 pKa = 4.14 NNNDD46 pKa = 3.43 NNNNNGIADD55 pKa = 4.08 SVGDD59 pKa = 3.97 GAPLSTQVTATDD71 pKa = 3.81 NNNDD75 pKa = 3.48 NNDD78 pKa = 3.39 IADD81 pKa = 3.61 QTIVEE86 pKa = 4.37 SHH88 pKa = 5.75 QLPNNDD94 pKa = 4.44 NNDD97 pKa = 2.94 IDD99 pKa = 3.93 QRR101 pKa = 11.84 SGNVPITPDD110 pKa = 3.81 DD111 pKa = 4.48 VISIQPKK118 pKa = 9.12 KK119 pKa = 9.98 QLEE122 pKa = 4.19 PTMSNNEE129 pKa = 3.8 NNKK132 pKa = 10.02 AFISTDD138 pKa = 3.47 DD139 pKa = 3.76 NNAAIADD146 pKa = 3.75 ASVQVGDD153 pKa = 4.63 DD154 pKa = 3.88 NEE156 pKa = 4.0 ILAQKK161 pKa = 10.19 RR162 pKa = 11.84 GYY164 pKa = 9.89 QRR166 pKa = 11.84 DD167 pKa = 3.29 EE168 pKa = 4.6 SEE170 pKa = 4.04 CHH172 pKa = 6.21 EE173 pKa = 4.6 YY174 pKa = 11.01 YY175 pKa = 11.13 CNFTCFDD182 pKa = 4.47 DD183 pKa = 4.72 LKK185 pKa = 10.76 CDD187 pKa = 3.43 SGEE190 pKa = 4.6 NICIMCFSCLVSSFTSVCISLCQSSASKK218 pKa = 10.86
Molecular weight: 23.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.49
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.541
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.126
Thurlkill 3.554
EMBOSS 3.694
Sillero 3.846
Patrickios 0.846
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|A0A1E4T4N6|A0A1E4T4N6_9ASCO G-patch domain-containing protein OS=[Candida] arabinofermentans NRRL YB-2248 OX=983967 GN=CANARDRAFT_6210 PE=3 SV=1
MM1 pKa = 7.94 PSQKK5 pKa = 10.41 SFRR8 pKa = 11.84 TKK10 pKa = 10.38 QKK12 pKa = 10.07 LAKK15 pKa = 9.76 AQNQNRR21 pKa = 11.84 PLPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TNNKK34 pKa = 8.41 IRR36 pKa = 11.84 YY37 pKa = 5.78 NAKK40 pKa = 8.42 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.6 WKK45 pKa = 8.07 RR46 pKa = 11.84 TKK48 pKa = 10.75 LGII51 pKa = 3.98
Molecular weight: 6.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.365
IPC2_protein 11.008
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.34
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.076
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 8.991
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5824
3
5827
2735454
49
5024
469.4
52.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.185 ± 0.031
1.193 ± 0.012
6.201 ± 0.026
6.52 ± 0.031
4.411 ± 0.021
5.094 ± 0.029
1.933 ± 0.013
6.908 ± 0.025
7.29 ± 0.03
9.808 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.065 ± 0.01
5.971 ± 0.026
4.209 ± 0.025
4.138 ± 0.034
3.873 ± 0.019
9.374 ± 0.038
5.939 ± 0.025
5.43 ± 0.022
0.96 ± 0.008
3.49 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here