Monkey B-lymphotropic papovavirus
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q90131|Q90131_9POLY Minor capsid protein OS=Monkey B-lymphotropic papovavirus OX=10574 PE=3 SV=1
MM1 pKa = 7.58 APQRR5 pKa = 11.84 KK6 pKa = 8.36 RR7 pKa = 11.84 QDD9 pKa = 3.48 GACKK13 pKa = 8.94 KK14 pKa = 7.79 TCPIPAPVPRR24 pKa = 11.84 LLVKK28 pKa = 10.8 GGVEE32 pKa = 3.97 VLEE35 pKa = 4.41 VRR37 pKa = 11.84 TGPDD41 pKa = 3.47 AITQIEE47 pKa = 4.77 AYY49 pKa = 10.29 LNPRR53 pKa = 11.84 MGNNIPSEE61 pKa = 4.0 DD62 pKa = 3.61 LYY64 pKa = 11.38 GYY66 pKa = 10.56 SNSINTAFSKK76 pKa = 11.09 ASDD79 pKa = 3.87 TPNKK83 pKa = 8.68 GTLPCYY89 pKa = 10.03 SVAVIKK95 pKa = 10.87 LPLLNEE101 pKa = 4.73 DD102 pKa = 3.9 MTCDD106 pKa = 3.74 TILMWEE112 pKa = 4.1 AVSVKK117 pKa = 9.88 TEE119 pKa = 3.84 VVGISSLVNLHH130 pKa = 6.16 QGGKK134 pKa = 9.56 YY135 pKa = 9.87 IYY137 pKa = 9.33 GSSSGCVPVQGTTYY151 pKa = 11.11 HH152 pKa = 5.69 MFAVGGEE159 pKa = 3.95 PLEE162 pKa = 4.2 LQGLVASSTATYY174 pKa = 9.73 PDD176 pKa = 3.86 DD177 pKa = 3.58 VVAIKK182 pKa = 10.66 NMKK185 pKa = 9.52 PGNQGLDD192 pKa = 3.51 PKK194 pKa = 11.16 AKK196 pKa = 10.51 ALLDD200 pKa = 3.67 KK201 pKa = 10.9 DD202 pKa = 3.32 GKK204 pKa = 10.91 YY205 pKa = 9.68 PVEE208 pKa = 4.34 VWCPDD213 pKa = 3.3 PSKK216 pKa = 11.43 NEE218 pKa = 3.45 NTRR221 pKa = 11.84 YY222 pKa = 10.01 YY223 pKa = 11.35 GSFTGGATTPPVMQFTNSVTTVLLDD248 pKa = 3.75 EE249 pKa = 5.12 NGVGPLCKK257 pKa = 9.66 GDD259 pKa = 4.92 KK260 pKa = 10.77 LFLSCADD267 pKa = 3.05 IAGVHH272 pKa = 5.36 TNYY275 pKa = 10.39 SEE277 pKa = 4.22 TQSWRR282 pKa = 11.84 GLPRR286 pKa = 11.84 YY287 pKa = 9.97 FNVTLRR293 pKa = 11.84 KK294 pKa = 9.82 RR295 pKa = 11.84 IVKK298 pKa = 9.99 NPYY301 pKa = 9.02 PVSSLLNSFFSGLMPQIQGQPMEE324 pKa = 4.52 GVSGQVEE331 pKa = 4.1 EE332 pKa = 4.1 VRR334 pKa = 11.84 IFEE337 pKa = 4.29 GTEE340 pKa = 3.96 GLPGDD345 pKa = 4.53 PDD347 pKa = 3.8 LNRR350 pKa = 11.84 YY351 pKa = 7.58 VDD353 pKa = 4.7 KK354 pKa = 10.92 FCQNQTVLPVSNDD367 pKa = 2.95 MM368 pKa = 4.89
Molecular weight: 39.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.254
IPC2_protein 5.474
IPC_protein 5.41
Toseland 5.397
ProMoST 5.588
Dawson 5.461
Bjellqvist 5.563
Wikipedia 5.359
Rodwell 5.385
Grimsley 5.347
Solomon 5.461
Lehninger 5.423
Nozaki 5.614
DTASelect 5.779
Thurlkill 5.499
EMBOSS 5.461
Sillero 5.69
Patrickios 3.757
IPC_peptide 5.474
IPC2_peptide 5.677
IPC2.peptide.svr19 5.741
Protein with the highest isoelectric point:
>tr|Q90131|Q90131_9POLY Minor capsid protein OS=Monkey B-lymphotropic papovavirus OX=10574 PE=3 SV=1
MM1 pKa = 7.48 ALVPWFPQVDD11 pKa = 3.83 YY12 pKa = 10.54 LFPGFTSFSYY22 pKa = 10.85 YY23 pKa = 10.78 LNAVLDD29 pKa = 3.69 WGEE32 pKa = 4.36 SLFHH36 pKa = 6.37 AVGRR40 pKa = 11.84 EE41 pKa = 4.04 VWRR44 pKa = 11.84 HH45 pKa = 5.29 LMRR48 pKa = 11.84 QATLQIGQATRR59 pKa = 11.84 AVAVRR64 pKa = 11.84 STNEE68 pKa = 3.61 LSHH71 pKa = 6.47 TLAQIAEE78 pKa = 4.22 NARR81 pKa = 11.84 WALTSGPVHH90 pKa = 7.08 IYY92 pKa = 10.89 SSVQDD97 pKa = 3.52 YY98 pKa = 10.9 YY99 pKa = 11.36 RR100 pKa = 11.84 YY101 pKa = 10.31 LPARR105 pKa = 11.84 NPIQLRR111 pKa = 11.84 QEE113 pKa = 3.8 YY114 pKa = 9.42 RR115 pKa = 11.84 NRR117 pKa = 11.84 GEE119 pKa = 4.36 PPPSRR124 pKa = 11.84 ADD126 pKa = 3.39 FEE128 pKa = 4.47 YY129 pKa = 10.79 QEE131 pKa = 4.08 NRR133 pKa = 11.84 EE134 pKa = 4.12 GQRR137 pKa = 11.84 ARR139 pKa = 11.84 RR140 pKa = 11.84 EE141 pKa = 3.93 LGYY144 pKa = 10.58 DD145 pKa = 3.48 EE146 pKa = 5.27 PRR148 pKa = 11.84 SGQYY152 pKa = 9.54 VEE154 pKa = 5.75 HH155 pKa = 5.94 YY156 pKa = 6.84 TAPGGAHH163 pKa = 5.58 QRR165 pKa = 11.84 VTQDD169 pKa = 2.16 WMLPLILGLYY179 pKa = 10.53 GDD181 pKa = 4.55 ITPTWEE187 pKa = 3.77 VEE189 pKa = 4.03 LNKK192 pKa = 10.48 LEE194 pKa = 4.31 KK195 pKa = 11.11 EE196 pKa = 3.78 EE197 pKa = 5.3 DD198 pKa = 3.85 GPSKK202 pKa = 10.61 KK203 pKa = 9.93 KK204 pKa = 10.56 ARR206 pKa = 11.84 RR207 pKa = 11.84 SMQKK211 pKa = 9.95 NMPYY215 pKa = 10.19 SRR217 pKa = 11.84 SRR219 pKa = 11.84 PQAPSKK225 pKa = 10.27 RR226 pKa = 11.84 RR227 pKa = 11.84 SRR229 pKa = 11.84 GARR232 pKa = 11.84 SKK234 pKa = 11.07 NRR236 pKa = 11.84 AA237 pKa = 3.21
Molecular weight: 27.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.783
IPC2_protein 9.458
IPC_protein 9.97
Toseland 9.706
ProMoST 9.663
Dawson 10.058
Bjellqvist 9.823
Wikipedia 10.321
Rodwell 10.145
Grimsley 10.175
Solomon 10.087
Lehninger 10.028
Nozaki 9.677
DTASelect 9.823
Thurlkill 9.853
EMBOSS 10.16
Sillero 9.955
Patrickios 4.94
IPC_peptide 10.072
IPC2_peptide 8.624
IPC2.peptide.svr19 8.403
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
605
237
368
302.5
33.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.281 ± 1.17
1.488 ± 0.806
4.463 ± 0.589
5.785 ± 0.752
2.645 ± 0.061
8.099 ± 0.96
1.488 ± 0.566
3.306 ± 0.42
4.793 ± 0.769
8.264 ± 0.134
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.479 ± 0.2
4.959 ± 0.63
7.769 ± 0.323
4.959 ± 0.743
6.446 ± 2.681
7.107 ± 0.036
5.785 ± 0.849
7.603 ± 1.377
1.488 ± 0.566
4.793 ± 0.604
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here