Beihai weivirus-like virus 9
Average proteome isoelectric point is 7.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KLA9|A0A1L3KLA9_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 9 OX=1922756 PE=4 SV=1
MM1 pKa = 7.49 TLPLSVANTTSLRR14 pKa = 11.84 DD15 pKa = 3.27 IEE17 pKa = 4.42 GWMFKK22 pKa = 10.04 IASEE26 pKa = 4.26 RR27 pKa = 11.84 PGMYY31 pKa = 9.49 TIRR34 pKa = 11.84 VRR36 pKa = 11.84 LPQRR40 pKa = 11.84 RR41 pKa = 11.84 CGSCIKK47 pKa = 9.75 LTRR50 pKa = 11.84 SPLDD54 pKa = 3.56 DD55 pKa = 3.54 MEE57 pKa = 4.35 EE58 pKa = 4.36 VVVHH62 pKa = 5.08 ITGEE66 pKa = 4.13 EE67 pKa = 3.86 FAKK70 pKa = 10.25 LDD72 pKa = 3.83 KK73 pKa = 11.27 VIMNQSYY80 pKa = 10.49 SEE82 pKa = 4.38 SKK84 pKa = 10.48 PKK86 pKa = 10.1 VQAAIIYY93 pKa = 7.7 QHH95 pKa = 7.13 ASTIWPSHH103 pKa = 5.74 TPDD106 pKa = 3.84 DD107 pKa = 3.72 VTMRR111 pKa = 11.84 TLGAWAITQYY121 pKa = 10.67 RR122 pKa = 11.84 CNGGWAKK129 pKa = 10.66 RR130 pKa = 11.84 LLKK133 pKa = 10.3 CIPCLQYY140 pKa = 9.57 MQKK143 pKa = 10.16 EE144 pKa = 4.11 VHH146 pKa = 6.71 LDD148 pKa = 3.57 LKK150 pKa = 9.91 GLRR153 pKa = 11.84 MPNRR157 pKa = 11.84 PVPPTPVEE165 pKa = 4.24 SEE167 pKa = 4.01 EE168 pKa = 4.26 SSAGGTNGSTVRR180 pKa = 11.84 TNTTATTAEE189 pKa = 4.82 LSSQPTVEE197 pKa = 4.25 SLPTITEE204 pKa = 3.97 HH205 pKa = 8.08 SDD207 pKa = 3.46 EE208 pKa = 4.23 EE209 pKa = 4.7 DD210 pKa = 3.17 EE211 pKa = 4.22 NARR214 pKa = 11.84 ANEE217 pKa = 4.28 TPGEE221 pKa = 3.88 AWARR225 pKa = 11.84 WLAAGPPRR233 pKa = 11.84 NEE235 pKa = 3.32 IGYY238 pKa = 9.76 VEE240 pKa = 4.87 LGHH243 pKa = 6.84 GNEE246 pKa = 4.75 DD247 pKa = 3.61 LHH249 pKa = 7.35 PLGGASAEE257 pKa = 4.16 DD258 pKa = 3.36 SAIRR262 pKa = 11.84 AEE264 pKa = 4.18 RR265 pKa = 11.84 QDD267 pKa = 3.48 RR268 pKa = 11.84 VLEE271 pKa = 4.04 NAAGVGVVGQSTQSEE286 pKa = 4.18 NAKK289 pKa = 10.29 QIVGVVSLPMSEE301 pKa = 4.41 PPNVYY306 pKa = 10.35 AKK308 pKa = 10.25 EE309 pKa = 4.14 AEE311 pKa = 4.32 NIQAAIDD318 pKa = 3.46 QRR320 pKa = 11.84 ITRR323 pKa = 11.84 KK324 pKa = 9.38 QRR326 pKa = 11.84 AFTANKK332 pKa = 9.02 EE333 pKa = 4.12 DD334 pKa = 4.41 KK335 pKa = 10.33 ILLGRR340 pKa = 11.84 LVSEE344 pKa = 5.78 AIGNDD349 pKa = 3.06 PRR351 pKa = 11.84 RR352 pKa = 11.84 SLFSTRR358 pKa = 11.84 RR359 pKa = 11.84 VTEE362 pKa = 3.25 WWEE365 pKa = 3.66 THH367 pKa = 6.25 LFGDD371 pKa = 4.67 LKK373 pKa = 10.58 SGKK376 pKa = 6.64 WTEE379 pKa = 3.93 EE380 pKa = 3.69 RR381 pKa = 11.84 LSRR384 pKa = 11.84 TIEE387 pKa = 4.09 GLCQRR392 pKa = 11.84 INPKK396 pKa = 10.28 FKK398 pKa = 10.58 LSCDD402 pKa = 3.16 IKK404 pKa = 10.84 LEE406 pKa = 3.94 PMPEE410 pKa = 4.1 GKK412 pKa = 9.46 PPRR415 pKa = 11.84 MLIADD420 pKa = 3.77 GDD422 pKa = 4.09 EE423 pKa = 4.48 GQVLALLTICCIEE436 pKa = 4.3 DD437 pKa = 4.39 LIKK440 pKa = 10.83 KK441 pKa = 8.76 HH442 pKa = 6.07 LPKK445 pKa = 9.82 KK446 pKa = 8.18 TIKK449 pKa = 10.87 GLGKK453 pKa = 8.5 RR454 pKa = 11.84 QAMEE458 pKa = 3.83 RR459 pKa = 11.84 VAQEE463 pKa = 3.57 LRR465 pKa = 11.84 APKK468 pKa = 9.86 AAYY471 pKa = 10.0 AKK473 pKa = 9.23 TRR475 pKa = 11.84 SAAKK479 pKa = 9.67 SGQYY483 pKa = 9.7 TGFSKK488 pKa = 10.1 MVPPGVTVFEE498 pKa = 4.86 GDD500 pKa = 3.03 GSAWDD505 pKa = 4.23 TTCSASLRR513 pKa = 11.84 DD514 pKa = 3.86 CVEE517 pKa = 3.82 NPVIVHH523 pKa = 5.77 VASILKK529 pKa = 9.26 VHH531 pKa = 6.18 MVQPEE536 pKa = 4.36 GWVDD540 pKa = 3.14 AHH542 pKa = 5.79 TEE544 pKa = 3.58 ISKK547 pKa = 9.34 MEE549 pKa = 4.01 KK550 pKa = 10.46 LLIIFKK556 pKa = 10.87 KK557 pKa = 9.99 NGEE560 pKa = 3.94 FRR562 pKa = 11.84 KK563 pKa = 10.54 YY564 pKa = 10.26 IIDD567 pKa = 4.74 AIRR570 pKa = 11.84 RR571 pKa = 11.84 SGHH574 pKa = 6.55 RR575 pKa = 11.84 GTSCLNWWTNFVCWHH590 pKa = 6.01 CAIFEE595 pKa = 4.16 KK596 pKa = 10.85 PEE598 pKa = 3.9 IFLDD602 pKa = 3.39 PDD604 pKa = 2.99 VRR606 pKa = 11.84 YY607 pKa = 10.02 GLDD610 pKa = 3.12 HH611 pKa = 6.55 SGVWRR616 pKa = 11.84 WIATAFEE623 pKa = 4.82 GDD625 pKa = 3.87 DD626 pKa = 4.63 SILSTTPRR634 pKa = 11.84 IDD636 pKa = 3.42 VKK638 pKa = 10.95 SEE640 pKa = 3.64 IYY642 pKa = 10.42 VSIMQRR648 pKa = 11.84 WEE650 pKa = 3.59 RR651 pKa = 11.84 LGFNMEE657 pKa = 3.45 IFIRR661 pKa = 11.84 EE662 pKa = 3.78 EE663 pKa = 3.6 RR664 pKa = 11.84 AKK666 pKa = 10.02 FTGYY670 pKa = 11.19 YY671 pKa = 8.32 MALDD675 pKa = 3.76 NDD677 pKa = 4.11 GTTGVLMPEE686 pKa = 4.11 VDD688 pKa = 3.17 RR689 pKa = 11.84 CFARR693 pKa = 11.84 SGVSCSPSMIEE704 pKa = 4.42 CFKK707 pKa = 11.21 KK708 pKa = 10.34 EE709 pKa = 4.35 DD710 pKa = 3.53 RR711 pKa = 11.84 TGCQSISRR719 pKa = 11.84 AAALSRR725 pKa = 11.84 AYY727 pKa = 9.65 EE728 pKa = 4.03 FAGLSPTISTKK739 pKa = 7.95 YY740 pKa = 9.87 LRR742 pKa = 11.84 FYY744 pKa = 11.19 EE745 pKa = 4.3 SMSVSTKK752 pKa = 9.47 VDD754 pKa = 3.28 RR755 pKa = 11.84 DD756 pKa = 3.25 LKK758 pKa = 10.65 MRR760 pKa = 11.84 TCGGDD765 pKa = 3.12 AEE767 pKa = 4.45 FSEE770 pKa = 4.82 KK771 pKa = 10.7 EE772 pKa = 3.65 IVAEE776 pKa = 4.1 INLKK780 pKa = 10.7 NGGAMSFDD788 pKa = 3.14 SSEE791 pKa = 4.29 RR792 pKa = 11.84 DD793 pKa = 2.93 RR794 pKa = 11.84 LAAVGFKK801 pKa = 9.67 CTEE804 pKa = 3.92 EE805 pKa = 3.99 EE806 pKa = 4.07 LSRR809 pKa = 11.84 FALRR813 pKa = 11.84 MWDD816 pKa = 3.6 YY817 pKa = 11.89 DD818 pKa = 5.03 LLKK821 pKa = 10.78 DD822 pKa = 3.12 WDD824 pKa = 4.1 GFRR827 pKa = 11.84 EE828 pKa = 4.13 SLPEE832 pKa = 3.87 SWRR835 pKa = 11.84 MAA837 pKa = 3.76
Molecular weight: 93.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.012
IPC2_protein 6.046
IPC_protein 6.084
Toseland 6.262
ProMoST 6.453
Dawson 6.364
Bjellqvist 6.389
Wikipedia 6.364
Rodwell 6.351
Grimsley 6.338
Solomon 6.364
Lehninger 6.364
Nozaki 6.649
DTASelect 6.795
Thurlkill 6.81
EMBOSS 6.781
Sillero 6.737
Patrickios 4.431
IPC_peptide 6.389
IPC2_peptide 6.634
IPC2.peptide.svr19 6.655
Protein with the highest isoelectric point:
>tr|A0A1L3KLA9|A0A1L3KLA9_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 9 OX=1922756 PE=4 SV=1
MM1 pKa = 7.04 VLHH4 pKa = 7.1 RR5 pKa = 11.84 GKK7 pKa = 10.23 KK8 pKa = 8.68 HH9 pKa = 5.47 AALGRR14 pKa = 11.84 KK15 pKa = 6.15 TAKK18 pKa = 9.0 THH20 pKa = 4.69 TKK22 pKa = 8.67 YY23 pKa = 9.77 ATRR26 pKa = 11.84 ARR28 pKa = 11.84 GVVSAAPPLTKK39 pKa = 10.57 GGIARR44 pKa = 11.84 HH45 pKa = 4.96 KK46 pKa = 10.57 AEE48 pKa = 3.79 RR49 pKa = 11.84 HH50 pKa = 4.79 KK51 pKa = 11.13 VISGYY56 pKa = 9.81 RR57 pKa = 11.84 RR58 pKa = 11.84 EE59 pKa = 3.98 EE60 pKa = 4.08 DD61 pKa = 3.37 KK62 pKa = 11.61 LNAFLPGHH70 pKa = 6.38 LTLPRR75 pKa = 11.84 AVAPYY80 pKa = 9.83 SVVRR84 pKa = 11.84 TIVNFPLACSDD95 pKa = 3.64 RR96 pKa = 11.84 GKK98 pKa = 9.51 MCVIQPMVNEE108 pKa = 4.41 DD109 pKa = 3.21 AGSNKK114 pKa = 8.32 ITFTNCIGWVSDD126 pKa = 3.58 TDD128 pKa = 3.68 QSSGPVPTTNTPLGINMPSVGNTGVEE154 pKa = 4.32 CVPAAISVRR163 pKa = 11.84 ITCPSPLIAATGQLFLGRR181 pKa = 11.84 WNMPADD187 pKa = 3.86 RR188 pKa = 11.84 KK189 pKa = 11.02 NYY191 pKa = 8.64 VHH193 pKa = 6.89 YY194 pKa = 11.13 DD195 pKa = 3.48 DD196 pKa = 4.69 MEE198 pKa = 5.72 KK199 pKa = 11.17 GFLAFAKK206 pKa = 9.96 PEE208 pKa = 4.4 PYY210 pKa = 8.75 TAAMMATCTRR220 pKa = 11.84 QVSAIPRR227 pKa = 11.84 DD228 pKa = 3.7 FNDD231 pKa = 3.01 YY232 pKa = 11.39 CEE234 pKa = 4.03 FHH236 pKa = 7.34 NYY238 pKa = 9.45 EE239 pKa = 4.37 DD240 pKa = 4.2 TNFTDD245 pKa = 5.26 ALKK248 pKa = 9.89 PWGGMTPILYY258 pKa = 10.07 CLSPVSGGNVTQYY271 pKa = 11.54 NLQICVEE278 pKa = 3.49 WRR280 pKa = 11.84 YY281 pKa = 9.8 RR282 pKa = 11.84 FKK284 pKa = 10.57 MDD286 pKa = 3.98 DD287 pKa = 3.54 PAASSHH293 pKa = 5.82 EE294 pKa = 3.93 FHH296 pKa = 7.08 FPHH299 pKa = 7.53 PIDD302 pKa = 3.98 KK303 pKa = 11.05 VNGLIARR310 pKa = 11.84 MSGKK314 pKa = 9.82 PGVDD318 pKa = 3.25 VVKK321 pKa = 10.68 KK322 pKa = 10.39 GDD324 pKa = 3.56 KK325 pKa = 10.59 SPPDD329 pKa = 3.78 VLTKK333 pKa = 10.83
Molecular weight: 36.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.676
IPC2_protein 8.507
IPC_protein 8.39
Toseland 8.916
ProMoST 8.99
Dawson 9.311
Bjellqvist 9.268
Wikipedia 9.428
Rodwell 9.472
Grimsley 9.253
Solomon 9.385
Lehninger 9.355
Nozaki 9.443
DTASelect 9.121
Thurlkill 9.238
EMBOSS 9.458
Sillero 9.428
Patrickios 4.635
IPC_peptide 9.37
IPC2_peptide 8.112
IPC2.peptide.svr19 7.776
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1170
333
837
585.0
65.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.034 ± 0.371
2.479 ± 0.123
5.299 ± 0.058
7.009 ± 2.036
3.248 ± 0.195
7.009 ± 0.274
2.222 ± 0.594
5.556 ± 0.578
6.068 ± 0.296
6.923 ± 0.669
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.077 ± 0.124
3.504 ± 0.715
5.812 ± 1.262
2.479 ± 0.372
6.838 ± 0.622
7.009 ± 0.881
6.581 ± 0.344
6.325 ± 0.815
2.051 ± 0.467
2.479 ± 0.453
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here