Suakwa aphid-borne yellows virus
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J7FDA2|J7FDA2_9LUTE Movement protein OS=Suakwa aphid-borne yellows virus OX=646010 PE=3 SV=1
MM1 pKa = 7.68 KK2 pKa = 10.62 NMQFVVLFFFCLSASTLCWPEE23 pKa = 3.86 VEE25 pKa = 4.72 PEE27 pKa = 4.15 SAQALYY33 pKa = 9.89 PGPTYY38 pKa = 10.82 NLSYY42 pKa = 11.02 DD43 pKa = 3.7 GLSVLEE49 pKa = 4.95 LPFHH53 pKa = 6.42 TDD55 pKa = 2.09 WKK57 pKa = 10.09 EE58 pKa = 3.58 IKK60 pKa = 10.12 EE61 pKa = 4.1 NFGFFCEE68 pKa = 4.56 EE69 pKa = 4.19 PGEE72 pKa = 4.3 LDD74 pKa = 3.74 LNQLIAEE81 pKa = 4.33 SSYY84 pKa = 11.51 TDD86 pKa = 2.99 ILTSLSNKK94 pKa = 9.46 ILLDD98 pKa = 3.99 FKK100 pKa = 11.18 SVVSVALDD108 pKa = 3.36 TFLVFWALCKK118 pKa = 10.78 GSIQAYY124 pKa = 9.64 AFRR127 pKa = 11.84 AFKK130 pKa = 10.52 AWLEE134 pKa = 4.16 VIITLWISVISAILSGLTWALLNIFPHH161 pKa = 5.79 TLTLIFLFFFTNLLVKK177 pKa = 9.83 GFRR180 pKa = 11.84 ILCGTACLAFLRR192 pKa = 11.84 MLFSPLLPVKK202 pKa = 10.54 RR203 pKa = 11.84 ILTWTLARR211 pKa = 11.84 LLGNSFGRR219 pKa = 11.84 KK220 pKa = 8.65 LEE222 pKa = 4.11 KK223 pKa = 9.99 ATEE226 pKa = 4.01 GWKK229 pKa = 10.61 SFEE232 pKa = 4.35 IKK234 pKa = 9.22 MAPPSSSALPVRR246 pKa = 11.84 NRR248 pKa = 11.84 ITKK251 pKa = 8.99 EE252 pKa = 3.38 HH253 pKa = 6.61 AGYY256 pKa = 10.49 CGFVQTHH263 pKa = 5.9 MKK265 pKa = 10.1 NADD268 pKa = 3.23 GTYY271 pKa = 9.78 VEE273 pKa = 4.89 AFITARR279 pKa = 11.84 HH280 pKa = 6.03 VIDD283 pKa = 5.74 DD284 pKa = 4.36 DD285 pKa = 4.02 LGEE288 pKa = 4.04 IHH290 pKa = 7.15 SPKK293 pKa = 9.44 TKK295 pKa = 10.85 NFIACADD302 pKa = 4.07 LEE304 pKa = 4.41 VLGDD308 pKa = 3.69 WEE310 pKa = 5.03 SIDD313 pKa = 5.9 LILLRR318 pKa = 11.84 APKK321 pKa = 9.79 HH322 pKa = 4.88 ARR324 pKa = 11.84 SYY326 pKa = 10.63 LGCKK330 pKa = 8.13 PASMVAIDD338 pKa = 5.47 RR339 pKa = 11.84 LATCDD344 pKa = 3.25 ASTFHH349 pKa = 7.27 LIDD352 pKa = 4.75 GEE354 pKa = 4.27 WHH356 pKa = 5.96 MSNARR361 pKa = 11.84 LVGDD365 pKa = 4.0 VKK367 pKa = 11.26 NGVAVLSNSVCGHH380 pKa = 6.13 SGSPFYY386 pKa = 10.59 NGKK389 pKa = 9.8 HH390 pKa = 5.36 ILGVLYY396 pKa = 10.86 GGGSYY401 pKa = 10.73 FEE403 pKa = 5.09 NFNVMNPIPAIPGLTTPEE421 pKa = 3.74 RR422 pKa = 11.84 RR423 pKa = 11.84 LEE425 pKa = 4.08 TTAPRR430 pKa = 11.84 GKK432 pKa = 10.02 IFNNEE437 pKa = 3.67 DD438 pKa = 3.22 FFQKK442 pKa = 10.79 LEE444 pKa = 4.01 EE445 pKa = 5.07 EE446 pKa = 4.36 YY447 pKa = 11.17 LQMKK451 pKa = 9.03 NFKK454 pKa = 10.58 SKK456 pKa = 9.56 TGVDD460 pKa = 3.43 WADD463 pKa = 3.69 LPSDD467 pKa = 4.92 DD468 pKa = 5.86 EE469 pKa = 4.77 MDD471 pKa = 4.01 FQDD474 pKa = 4.53 ALPSFRR480 pKa = 11.84 KK481 pKa = 9.84 AEE483 pKa = 4.09 KK484 pKa = 10.43 AEE486 pKa = 4.2 PSHH489 pKa = 6.61 PVANTRR495 pKa = 11.84 EE496 pKa = 4.08 PAVVVGDD503 pKa = 3.63 IRR505 pKa = 11.84 CNISGNRR512 pKa = 11.84 RR513 pKa = 11.84 EE514 pKa = 4.12 QGRR517 pKa = 11.84 LLQQRR522 pKa = 11.84 PHH524 pKa = 5.64 VRR526 pKa = 11.84 CCEE529 pKa = 4.03 EE530 pKa = 3.87 PRR532 pKa = 11.84 EE533 pKa = 4.31 SCGPDD538 pKa = 2.98 GGQCGHH544 pKa = 7.37 PGDD547 pKa = 4.09 PTRR550 pKa = 11.84 GSSDD554 pKa = 3.37 SCCPALRR561 pKa = 11.84 QPEE564 pKa = 4.19 KK565 pKa = 10.91 DD566 pKa = 3.28 EE567 pKa = 4.42 SRR569 pKa = 11.84 TVPAWEE575 pKa = 4.23 SRR577 pKa = 11.84 GTEE580 pKa = 3.29 ADD582 pKa = 2.76 NFRR585 pKa = 11.84 RR586 pKa = 11.84 YY587 pKa = 8.43 FQNEE591 pKa = 3.81 YY592 pKa = 9.83 DD593 pKa = 3.58 WEE595 pKa = 4.38 VRR597 pKa = 11.84 APAPQVPGFRR607 pKa = 11.84 HH608 pKa = 6.55 CGTLPRR614 pKa = 11.84 YY615 pKa = 9.32 YY616 pKa = 10.67 FSRR619 pKa = 11.84 QKK621 pKa = 11.4 GEE623 pKa = 4.26 TEE625 pKa = 3.39 WGKK628 pKa = 7.87 TLTRR632 pKa = 11.84 DD633 pKa = 3.51 NPSLGEE639 pKa = 3.88 ATAGYY644 pKa = 8.93 GWPEE648 pKa = 4.22 FGPGAEE654 pKa = 4.26 LKK656 pKa = 10.47 SLKK659 pKa = 10.2 LQAARR664 pKa = 11.84 WRR666 pKa = 11.84 EE667 pKa = 3.81 RR668 pKa = 11.84 ASSAKK673 pKa = 9.91 VPYY676 pKa = 8.82 PSEE679 pKa = 4.07 RR680 pKa = 11.84 EE681 pKa = 3.76 RR682 pKa = 11.84 VIRR685 pKa = 11.84 IATEE689 pKa = 4.12 RR690 pKa = 11.84 YY691 pKa = 9.28 SKK693 pKa = 10.77 VKK695 pKa = 10.42 SVAPACSRR703 pKa = 11.84 SGEE706 pKa = 4.28 LKK708 pKa = 10.16 WEE710 pKa = 4.15 GLVEE714 pKa = 4.21 DD715 pKa = 5.59 LKK717 pKa = 11.53 DD718 pKa = 3.96 CLPSLSLEE726 pKa = 4.37 AGVGVPYY733 pKa = 10.23 IAYY736 pKa = 9.16 GRR738 pKa = 11.84 PTLGSWVNDD747 pKa = 3.36 QALLPILSRR756 pKa = 11.84 LVFDD760 pKa = 4.62 RR761 pKa = 11.84 LKK763 pKa = 11.13 KK764 pKa = 9.41 MSEE767 pKa = 3.83 TRR769 pKa = 11.84 FDD771 pKa = 4.17 DD772 pKa = 3.59 MTPEE776 pKa = 3.95 EE777 pKa = 4.43 LVQNGLCDD785 pKa = 4.91 PIRR788 pKa = 11.84 TFIKK792 pKa = 10.71 GEE794 pKa = 3.74 PHH796 pKa = 6.74 KK797 pKa = 10.88 SSKK800 pKa = 10.55 LKK802 pKa = 9.58 EE803 pKa = 3.75 GRR805 pKa = 11.84 YY806 pKa = 9.15 RR807 pKa = 11.84 LIMSVSIVDD816 pKa = 3.48 QLVARR821 pKa = 11.84 VLFQNQNKK829 pKa = 10.05 SEE831 pKa = 4.05 IALWRR836 pKa = 11.84 AIPSKK841 pKa = 10.48 PGLGLSTDD849 pKa = 3.62 EE850 pKa = 3.99 QVLDD854 pKa = 4.63 FVHH857 pKa = 6.47 NLAEE861 pKa = 3.99 NTEE864 pKa = 4.11 YY865 pKa = 10.84 RR866 pKa = 11.84 EE867 pKa = 4.15 SVEE870 pKa = 5.28 DD871 pKa = 5.81 FIKK874 pKa = 9.87 HH875 pKa = 3.86 WHH877 pKa = 5.55 RR878 pKa = 11.84 TVVPTDD884 pKa = 3.24 CSGFDD889 pKa = 3.34 WSVSSWMLEE898 pKa = 3.48 DD899 pKa = 4.98 DD900 pKa = 3.64 MEE902 pKa = 4.34 VRR904 pKa = 11.84 NNLTINNNEE913 pKa = 3.64 LTRR916 pKa = 11.84 RR917 pKa = 11.84 LRR919 pKa = 11.84 ACWLKK924 pKa = 10.87 CISNSVICLSDD935 pKa = 3.39 GTLLAQEE942 pKa = 4.58 FPGIQKK948 pKa = 10.23 SGSYY952 pKa = 8.29 NTSSTNSRR960 pKa = 11.84 VRR962 pKa = 11.84 VMLSIYY968 pKa = 10.65 AGASWAIAMGDD979 pKa = 3.83 DD980 pKa = 3.85 ALEE983 pKa = 4.36 SADD986 pKa = 4.21 TIIPLYY992 pKa = 9.06 EE993 pKa = 3.85 QYY995 pKa = 11.12 GLKK998 pKa = 10.38 VEE1000 pKa = 4.48 VSDD1003 pKa = 3.87 KK1004 pKa = 11.67 LEE1006 pKa = 4.38 FCSHH1010 pKa = 5.84 EE1011 pKa = 4.46 FKK1013 pKa = 10.94 SSSLAIPTNIGKK1025 pKa = 8.31 MLYY1028 pKa = 10.21 KK1029 pKa = 10.39 LIHH1032 pKa = 6.61 GYY1034 pKa = 10.48 EE1035 pKa = 4.06 IFPGMSSIQVTINYY1049 pKa = 7.63 LTACYY1054 pKa = 10.21 SVLNEE1059 pKa = 4.0 LRR1061 pKa = 11.84 HH1062 pKa = 5.29 VPEE1065 pKa = 4.4 AASLVAQCLAPVEE1078 pKa = 4.18 AQKK1081 pKa = 11.17 VV1082 pKa = 3.51
Molecular weight: 121.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.687
IPC2_protein 5.728
IPC_protein 5.753
Toseland 6.008
ProMoST 6.084
Dawson 5.982
Bjellqvist 6.02
Wikipedia 5.982
Rodwell 5.97
Grimsley 6.097
Solomon 5.982
Lehninger 5.982
Nozaki 6.249
DTASelect 6.415
Thurlkill 6.415
EMBOSS 6.364
Sillero 6.338
Patrickios 4.101
IPC_peptide 6.008
IPC2_peptide 6.326
IPC2.peptide.svr19 6.34
Protein with the highest isoelectric point:
>tr|J7FE76|J7FE76_9LUTE p3 OS=Suakwa aphid-borne yellows virus OX=646010 PE=4 SV=1
MM1 pKa = 6.53 NTVVVRR7 pKa = 11.84 NRR9 pKa = 11.84 NGMGRR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 NSRR20 pKa = 11.84 RR21 pKa = 11.84 NAKK24 pKa = 9.19 GNRR27 pKa = 11.84 VVVVQAPGAPQRR39 pKa = 11.84 GRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 NGRR48 pKa = 11.84 RR49 pKa = 11.84 PSRR52 pKa = 11.84 GGRR55 pKa = 11.84 ARR57 pKa = 11.84 GTSQGEE63 pKa = 4.12 TFVFSKK69 pKa = 11.24 DD70 pKa = 3.3 NLTGSSSGAITFGPSLSDD88 pKa = 3.25 SPAFSSGILKK98 pKa = 10.26 AYY100 pKa = 10.18 HH101 pKa = 6.62 EE102 pKa = 4.74 YY103 pKa = 10.05 KK104 pKa = 10.27 ISMVKK109 pKa = 10.66 LEE111 pKa = 5.07 FISEE115 pKa = 4.18 ASSTSSGSISYY126 pKa = 10.24 EE127 pKa = 3.75 LDD129 pKa = 3.23 PHH131 pKa = 6.86 CKK133 pKa = 10.08 LSSLQSTVNKK143 pKa = 10.33 FGITKK148 pKa = 10.09 SGSRR152 pKa = 11.84 TWSAKK157 pKa = 9.97 FINGMEE163 pKa = 3.99 WHH165 pKa = 7.45 DD166 pKa = 3.56 ATEE169 pKa = 4.04 DD170 pKa = 3.41 QFRR173 pKa = 11.84 ILYY176 pKa = 9.14 KK177 pKa = 11.03 GNGSSSTAGSFRR189 pKa = 11.84 ITIRR193 pKa = 11.84 CQVQNPKK200 pKa = 10.62
Molecular weight: 22.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.19
IPC2_protein 10.087
IPC_protein 11.359
Toseland 11.52
ProMoST 11.93
Dawson 11.535
Bjellqvist 11.477
Wikipedia 11.959
Rodwell 11.433
Grimsley 11.564
Solomon 11.959
Lehninger 11.871
Nozaki 11.506
DTASelect 11.477
Thurlkill 11.506
EMBOSS 11.989
Sillero 11.52
Patrickios 11.155
IPC_peptide 11.974
IPC2_peptide 10.935
IPC2.peptide.svr19 9.253
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3062
192
1082
510.3
56.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.218 ± 0.499
2.025 ± 0.393
4.605 ± 0.242
6.434 ± 0.392
4.474 ± 0.582
7.185 ± 0.679
2.057 ± 0.094
4.931 ± 0.203
4.899 ± 0.415
9.014 ± 1.251
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.731 ± 0.1
4.442 ± 0.227
5.944 ± 0.414
3.135 ± 0.328
6.793 ± 0.72
9.242 ± 0.936
5.519 ± 0.272
5.487 ± 0.307
1.992 ± 0.157
2.841 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here