Bacillus phage vB_BtS_BMBtp13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1P760|A0A1B1P760_9CAUD Uncharacterized protein OS=Bacillus phage vB_BtS_BMBtp13 OX=1868825 GN=BMBtpLA1_15 PE=4 SV=1
MM1 pKa = 7.28NEE3 pKa = 3.82KK4 pKa = 10.23SKK6 pKa = 11.34KK7 pKa = 9.83LMKK10 pKa = 9.76EE11 pKa = 3.46QRR13 pKa = 11.84SGIKK17 pKa = 9.78KK18 pKa = 9.99ALEE21 pKa = 4.52DD22 pKa = 3.87GFKK25 pKa = 10.98LLVVEE30 pKa = 5.72DD31 pKa = 4.42EE32 pKa = 4.24LAEE35 pKa = 4.49DD36 pKa = 4.55EE37 pKa = 4.48EE38 pKa = 4.8SQLTEE43 pKa = 3.4EE44 pKa = 5.51GYY46 pKa = 10.7NCFILEE52 pKa = 4.1YY53 pKa = 11.27GEE55 pKa = 4.48FQPSSNDD62 pKa = 3.08RR63 pKa = 11.84TISQSIYY70 pKa = 10.57VSYY73 pKa = 10.9LSEE76 pKa = 4.12NQSNLDD82 pKa = 3.55EE83 pKa = 4.07QVIDD87 pKa = 4.01IISWVGKK94 pKa = 9.04VKK96 pKa = 9.79MVSFVVSKK104 pKa = 10.73SDD106 pKa = 3.19RR107 pKa = 11.84LQVKK111 pKa = 8.79DD112 pKa = 3.24TDD114 pKa = 3.72RR115 pKa = 11.84FIDD118 pKa = 3.38RR119 pKa = 11.84VVFTFKK125 pKa = 10.48RR126 pKa = 11.84VIPIEE131 pKa = 4.47CII133 pKa = 3.28

Molecular weight: 15.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1P741|A0A1B1P741_9CAUD Uncharacterized protein OS=Bacillus phage vB_BtS_BMBtp13 OX=1868825 GN=BMBtpLA1_4 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 10.32VLKK5 pKa = 10.5DD6 pKa = 4.1QLRR9 pKa = 11.84EE10 pKa = 3.8WKK12 pKa = 9.93KK13 pKa = 10.03QSKK16 pKa = 9.44KK17 pKa = 9.68VKK19 pKa = 10.02KK20 pKa = 10.11KK21 pKa = 10.08NKK23 pKa = 9.34KK24 pKa = 7.87KK25 pKa = 10.33QKK27 pKa = 9.56EE28 pKa = 4.16KK29 pKa = 10.97LSTRR33 pKa = 11.84DD34 pKa = 3.12IEE36 pKa = 4.48DD37 pKa = 3.02LMGIHH42 pKa = 7.05GPRR45 pKa = 11.84YY46 pKa = 7.28EE47 pKa = 3.92RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84GAVRR54 pKa = 11.84QKK56 pKa = 11.16

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30

0

30

7481

56

948

249.4

28.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.309 ± 0.742

0.909 ± 0.273

5.748 ± 0.341

7.459 ± 0.555

4.237 ± 0.365

7.018 ± 0.508

1.216 ± 0.188

7.873 ± 0.792

8.903 ± 0.692

8.234 ± 0.56

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.914 ± 0.223

6.109 ± 0.347

2.807 ± 0.298

4.224 ± 0.519

4.331 ± 0.454

5.374 ± 0.209

5.106 ± 0.263

6.523 ± 0.343

0.989 ± 0.139

3.716 ± 0.488
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski