Amino acid dipepetide frequency for Bacillus salacetis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.735AlaAla: 6.735 ± 0.102	0.533AlaCys: 0.533 ± 0.023	3.705AlaAsp: 3.705 ± 0.058	5.246AlaGlu: 5.246 ± 0.076	3.298AlaPhe: 3.298 ± 0.056	6.067AlaGly: 6.067 ± 0.087	1.183AlaHis: 1.183 ± 0.036	5.055AlaIle: 5.055 ± 0.068	4.258AlaLys: 4.258 ± 0.061	6.883AlaLeu: 6.883 ± 0.077	1.898AlaMet: 1.898 ± 0.041	2.349AlaAsn: 2.349 ± 0.049	2.039AlaPro: 2.039 ± 0.047	2.073AlaGln: 2.073 ± 0.045	2.62AlaArg: 2.62 ± 0.048	4.051AlaSer: 4.051 ± 0.056	2.611AlaThr: 2.611 ± 0.058	5.869AlaVal: 5.869 ± 0.074	0.595AlaTrp: 0.595 ± 0.024	2.157AlaTyr: 2.157 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	0.366CysAla: 0.366 ± 0.017	0.087CysCys: 0.087 ± 0.009	0.372CysAsp: 0.372 ± 0.018	0.445CysGlu: 0.445 ± 0.021	0.324CysPhe: 0.324 ± 0.015	0.631CysGly: 0.631 ± 0.026	0.183CysHis: 0.183 ± 0.012	0.477CysIle: 0.477 ± 0.02	0.323CysLys: 0.323 ± 0.018	0.642CysLeu: 0.642 ± 0.025	0.184CysMet: 0.184 ± 0.013	0.242CysAsn: 0.242 ± 0.017	0.331CysPro: 0.331 ± 0.019	0.19CysGln: 0.19 ± 0.012	0.342CysArg: 0.342 ± 0.017	0.533CysSer: 0.533 ± 0.021	0.369CysThr: 0.369 ± 0.015	0.369CysVal: 0.369 ± 0.016	0.07CysTrp: 0.07 ± 0.008	0.25CysTyr: 0.25 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.258AspAla: 3.258 ± 0.059	0.393AspCys: 0.393 ± 0.018	2.521AspAsp: 2.521 ± 0.048	4.729AspGlu: 4.729 ± 0.064	2.562AspPhe: 2.562 ± 0.05	3.53AspGly: 3.53 ± 0.061	1.156AspHis: 1.156 ± 0.03	4.254AspIle: 4.254 ± 0.059	3.263AspLys: 3.263 ± 0.056	5.197AspLeu: 5.197 ± 0.067	1.467AspMet: 1.467 ± 0.034	1.927AspAsn: 1.927 ± 0.048	2.076AspPro: 2.076 ± 0.042	1.948AspGln: 1.948 ± 0.046	2.505AspArg: 2.505 ± 0.05	3.223AspSer: 3.223 ± 0.052	2.513AspThr: 2.513 ± 0.051	3.435AspVal: 3.435 ± 0.053	0.697AspTrp: 0.697 ± 0.023	2.209AspTyr: 2.209 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	5.59GluAla: 5.59 ± 0.073	0.393GluCys: 0.393 ± 0.019	4.34GluAsp: 4.34 ± 0.069	8.312GluGlu: 8.312 ± 0.104	2.864GluPhe: 2.864 ± 0.049	5.172GluGly: 5.172 ± 0.059	1.383GluHis: 1.383 ± 0.034	5.714GluIle: 5.714 ± 0.07	7.081GluLys: 7.081 ± 0.092	7.433GluLeu: 7.433 ± 0.086	2.575GluMet: 2.575 ± 0.043	4.079GluAsn: 4.079 ± 0.064	2.027GluPro: 2.027 ± 0.048	2.972GluGln: 2.972 ± 0.056	3.599GluArg: 3.599 ± 0.062	4.077GluSer: 4.077 ± 0.078	4.001GluThr: 4.001 ± 0.059	5.3GluVal: 5.3 ± 0.061	0.955GluTrp: 0.955 ± 0.026	2.467GluTyr: 2.467 ± 0.052	0.0GluXaa: 0.0 ± 0.0
Phe	3.005PheAla: 3.005 ± 0.061	0.333PheCys: 0.333 ± 0.018	2.419PheAsp: 2.419 ± 0.05	3.02PheGlu: 3.02 ± 0.048	2.512PhePhe: 2.512 ± 0.057	3.309PheGly: 3.309 ± 0.067	1.039PheHis: 1.039 ± 0.028	3.863PheIle: 3.863 ± 0.062	2.55PheLys: 2.55 ± 0.047	4.923PheLeu: 4.923 ± 0.085	1.26PheMet: 1.26 ± 0.033	1.966PheAsn: 1.966 ± 0.039	1.722PhePro: 1.722 ± 0.041	1.687PheGln: 1.687 ± 0.04	1.674PheArg: 1.674 ± 0.037	3.575PheSer: 3.575 ± 0.051	2.727PheThr: 2.727 ± 0.048	2.93PheVal: 2.93 ± 0.048	0.525PheTrp: 0.525 ± 0.02	1.828PheTyr: 1.828 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	4.789GlyAla: 4.789 ± 0.091	0.642GlyCys: 0.642 ± 0.023	3.459GlyAsp: 3.459 ± 0.064	5.054GlyGlu: 5.054 ± 0.074	3.577GlyPhe: 3.577 ± 0.046	5.076GlyGly: 5.076 ± 0.091	1.33GlyHis: 1.33 ± 0.036	5.896GlyIle: 5.896 ± 0.077	5.148GlyLys: 5.148 ± 0.063	6.658GlyLeu: 6.658 ± 0.086	2.289GlyMet: 2.289 ± 0.048	2.813GlyAsn: 2.813 ± 0.055	1.743GlyPro: 1.743 ± 0.047	2.165GlyGln: 2.165 ± 0.044	3.118GlyArg: 3.118 ± 0.051	4.425GlySer: 4.425 ± 0.06	4.13GlyThr: 4.13 ± 0.065	4.951GlyVal: 4.951 ± 0.068	0.872GlyTrp: 0.872 ± 0.038	2.875GlyTyr: 2.875 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.15HisAla: 1.15 ± 0.029	0.174HisCys: 0.174 ± 0.012	1.019HisAsp: 1.019 ± 0.03	1.412HisGlu: 1.412 ± 0.035	1.124HisPhe: 1.124 ± 0.033	1.357HisGly: 1.357 ± 0.043	0.623HisHis: 0.623 ± 0.026	1.382HisIle: 1.382 ± 0.037	1.057HisLys: 1.057 ± 0.032	2.097HisLeu: 2.097 ± 0.045	0.477HisMet: 0.477 ± 0.017	0.763HisAsn: 0.763 ± 0.026	1.074HisPro: 1.074 ± 0.033	0.78HisGln: 0.78 ± 0.027	0.92HisArg: 0.92 ± 0.029	1.335HisSer: 1.335 ± 0.037	1.008HisThr: 1.008 ± 0.03	1.179HisVal: 1.179 ± 0.028	0.23HisTrp: 0.23 ± 0.014	0.865HisTyr: 0.865 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.388IleAla: 5.388 ± 0.069	0.552IleCys: 0.552 ± 0.019	4.173IleAsp: 4.173 ± 0.062	5.702IleGlu: 5.702 ± 0.078	3.284IlePhe: 3.284 ± 0.065	5.684IleGly: 5.684 ± 0.077	1.601IleHis: 1.601 ± 0.039	5.691IleIle: 5.691 ± 0.088	4.476IleLys: 4.476 ± 0.058	7.402IleLeu: 7.402 ± 0.094	1.81IleMet: 1.81 ± 0.041	3.104IleAsn: 3.104 ± 0.046	3.337IlePro: 3.337 ± 0.062	2.663IleGln: 2.663 ± 0.045	2.995IleArg: 2.995 ± 0.053	5.164IleSer: 5.164 ± 0.071	4.006IleThr: 4.006 ± 0.063	4.854IleVal: 4.854 ± 0.077	0.643IleTrp: 0.643 ± 0.025	2.404IleTyr: 2.404 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	4.839LysAla: 4.839 ± 0.064	0.322LysCys: 0.322 ± 0.016	4.063LysAsp: 4.063 ± 0.058	7.259LysGlu: 7.259 ± 0.092	1.998LysPhe: 1.998 ± 0.039	4.75LysGly: 4.75 ± 0.071	1.239LysHis: 1.239 ± 0.035	4.301LysIle: 4.301 ± 0.064	5.719LysLys: 5.719 ± 0.076	5.791LysLeu: 5.791 ± 0.069	2.141LysMet: 2.141 ± 0.04	3.182LysAsn: 3.182 ± 0.055	2.282LysPro: 2.282 ± 0.044	2.782LysGln: 2.782 ± 0.053	3.322LysArg: 3.322 ± 0.055	3.644LysSer: 3.644 ± 0.057	3.469LysThr: 3.469 ± 0.051	4.724LysVal: 4.724 ± 0.065	0.85LysTrp: 0.85 ± 0.028	2.102LysTyr: 2.102 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	7.131LeuAla: 7.131 ± 0.091	0.569LeuCys: 0.569 ± 0.022	4.871LeuAsp: 4.871 ± 0.065	7.081LeuGlu: 7.081 ± 0.086	5.039LeuPhe: 5.039 ± 0.077	6.252LeuGly: 6.252 ± 0.077	1.88LeuHis: 1.88 ± 0.044	7.265LeuIle: 7.265 ± 0.102	6.869LeuLys: 6.869 ± 0.08	10.343LeuLeu: 10.343 ± 0.12	2.664LeuMet: 2.664 ± 0.04	4.18LeuAsn: 4.18 ± 0.053	3.886LeuPro: 3.886 ± 0.057	3.605LeuGln: 3.605 ± 0.049	3.658LeuArg: 3.658 ± 0.057	7.038LeuSer: 7.038 ± 0.082	5.362LeuThr: 5.362 ± 0.061	5.935LeuVal: 5.935 ± 0.077	0.84LeuTrp: 0.84 ± 0.027	3.204LeuTyr: 3.204 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	2.107MetAla: 2.107 ± 0.038	0.126MetCys: 0.126 ± 0.011	1.628MetAsp: 1.628 ± 0.04	2.272MetGlu: 2.272 ± 0.042	1.111MetPhe: 1.111 ± 0.031	1.861MetGly: 1.861 ± 0.044	0.426MetHis: 0.426 ± 0.02	2.121MetIle: 2.121 ± 0.044	2.716MetLys: 2.716 ± 0.047	2.496MetLeu: 2.496 ± 0.048	0.87MetMet: 0.87 ± 0.033	1.653MetAsn: 1.653 ± 0.038	1.009MetPro: 1.009 ± 0.038	0.821MetGln: 0.821 ± 0.027	1.073MetArg: 1.073 ± 0.029	1.636MetSer: 1.636 ± 0.033	1.627MetThr: 1.627 ± 0.034	1.785MetVal: 1.785 ± 0.044	0.213MetTrp: 0.213 ± 0.014	0.714MetTyr: 0.714 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.626AsnAla: 2.626 ± 0.046	0.3AsnCys: 0.3 ± 0.015	2.238AsnAsp: 2.238 ± 0.044	3.534AsnGlu: 3.534 ± 0.065	1.615AsnPhe: 1.615 ± 0.035	3.432AsnGly: 3.432 ± 0.057	1.021AsnHis: 1.021 ± 0.031	3.195AsnIle: 3.195 ± 0.057	2.695AsnLys: 2.695 ± 0.05	3.778AsnLeu: 3.778 ± 0.054	1.124AsnMet: 1.124 ± 0.032	1.896AsnAsn: 1.896 ± 0.049	2.153AsnPro: 2.153 ± 0.046	1.751AsnGln: 1.751 ± 0.043	2.045AsnArg: 2.045 ± 0.044	2.505AsnSer: 2.505 ± 0.055	2.076AsnThr: 2.076 ± 0.038	2.703AsnVal: 2.703 ± 0.046	0.525AsnTrp: 0.525 ± 0.023	1.478AsnTyr: 1.478 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	2.602ProAla: 2.602 ± 0.046	0.225ProCys: 0.225 ± 0.013	2.27ProAsp: 2.27 ± 0.041	3.367ProGlu: 3.367 ± 0.06	2.062ProPhe: 2.062 ± 0.043	2.624ProGly: 2.624 ± 0.058	0.788ProHis: 0.788 ± 0.028	2.337ProIle: 2.337 ± 0.046	1.988ProLys: 1.988 ± 0.039	3.422ProLeu: 3.422 ± 0.063	0.825ProMet: 0.825 ± 0.026	1.411ProAsn: 1.411 ± 0.036	1.051ProPro: 1.051 ± 0.031	1.229ProGln: 1.229 ± 0.037	1.123ProArg: 1.123 ± 0.032	2.309ProSer: 2.309 ± 0.042	1.466ProThr: 1.466 ± 0.039	3.11ProVal: 3.11 ± 0.057	0.365ProTrp: 0.365 ± 0.019	1.419ProTyr: 1.419 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	2.426GlnAla: 2.426 ± 0.048	0.187GlnCys: 0.187 ± 0.012	1.781GlnAsp: 1.781 ± 0.037	3.007GlnGlu: 3.007 ± 0.064	1.543GlnPhe: 1.543 ± 0.035	2.348GlnGly: 2.348 ± 0.066	0.709GlnHis: 0.709 ± 0.026	2.204GlnIle: 2.204 ± 0.042	2.682GlnLys: 2.682 ± 0.049	3.417GlnLeu: 3.417 ± 0.06	1.082GlnMet: 1.082 ± 0.03	1.531GlnAsn: 1.531 ± 0.038	1.259GlnPro: 1.259 ± 0.035	1.578GlnGln: 1.578 ± 0.044	1.623GlnArg: 1.623 ± 0.036	2.2GlnSer: 2.2 ± 0.054	1.841GlnThr: 1.841 ± 0.038	2.11GlnVal: 2.11 ± 0.039	0.454GlnTrp: 0.454 ± 0.018	1.315GlnTyr: 1.315 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	2.319ArgAla: 2.319 ± 0.047	0.25ArgCys: 0.25 ± 0.013	2.166ArgAsp: 2.166 ± 0.047	3.547ArgGlu: 3.547 ± 0.062	2.05ArgPhe: 2.05 ± 0.046	2.536ArgGly: 2.536 ± 0.046	0.807ArgHis: 0.807 ± 0.027	3.19ArgIle: 3.19 ± 0.058	3.5ArgLys: 3.5 ± 0.062	3.991ArgLeu: 3.991 ± 0.063	1.419ArgMet: 1.419 ± 0.037	2.128ArgAsn: 2.128 ± 0.04	1.283ArgPro: 1.283 ± 0.032	1.516ArgGln: 1.516 ± 0.039	2.043ArgArg: 2.043 ± 0.051	2.453ArgSer: 2.453 ± 0.044	2.182ArgThr: 2.182 ± 0.044	2.574ArgVal: 2.574 ± 0.044	0.461ArgTrp: 0.461 ± 0.02	1.554ArgTyr: 1.554 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	4.225SerAla: 4.225 ± 0.058	0.408SerCys: 0.408 ± 0.018	3.056SerAsp: 3.056 ± 0.047	4.44SerGlu: 4.44 ± 0.058	3.513SerPhe: 3.513 ± 0.058	4.891SerGly: 4.891 ± 0.073	1.287SerHis: 1.287 ± 0.031	5.132SerIle: 5.132 ± 0.069	3.794SerLys: 3.794 ± 0.058	6.627SerLeu: 6.627 ± 0.08	1.857SerMet: 1.857 ± 0.042	2.378SerAsn: 2.378 ± 0.048	2.337SerPro: 2.337 ± 0.048	2.103SerGln: 2.103 ± 0.038	2.703SerArg: 2.703 ± 0.049	4.429SerSer: 4.429 ± 0.072	3.042SerThr: 3.042 ± 0.054	4.215SerVal: 4.215 ± 0.06	0.669SerTrp: 0.669 ± 0.026	2.312SerTyr: 2.312 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	4.138ThrAla: 4.138 ± 0.062	0.289ThrCys: 0.289 ± 0.016	2.752ThrAsp: 2.752 ± 0.052	3.633ThrGlu: 3.633 ± 0.062	2.496ThrPhe: 2.496 ± 0.042	4.339ThrGly: 4.339 ± 0.073	0.962ThrHis: 0.962 ± 0.03	4.079ThrIle: 4.079 ± 0.064	2.952ThrLys: 2.952 ± 0.05	5.058ThrLeu: 5.058 ± 0.062	1.233ThrMet: 1.233 ± 0.03	2.064ThrAsn: 2.064 ± 0.046	2.119ThrPro: 2.119 ± 0.046	1.299ThrGln: 1.299 ± 0.03	1.814ThrArg: 1.814 ± 0.043	3.163ThrSer: 3.163 ± 0.05	2.476ThrThr: 2.476 ± 0.046	3.953ThrVal: 3.953 ± 0.063	0.496ThrTrp: 0.496 ± 0.021	1.778ThrTyr: 1.778 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	4.01ValAla: 4.01 ± 0.072	0.534ValCys: 0.534 ± 0.022	3.582ValAsp: 3.582 ± 0.055	4.87ValGlu: 4.87 ± 0.071	3.364ValPhe: 3.364 ± 0.06	3.963ValGly: 3.963 ± 0.068	1.37ValHis: 1.37 ± 0.037	5.47ValIle: 5.47 ± 0.077	4.684ValLys: 4.684 ± 0.059	6.848ValLeu: 6.848 ± 0.072	1.868ValMet: 1.868 ± 0.037	2.973ValAsn: 2.973 ± 0.049	2.599ValPro: 2.599 ± 0.054	2.292ValGln: 2.292 ± 0.047	2.675ValArg: 2.675 ± 0.049	4.693ValSer: 4.693 ± 0.057	3.856ValThr: 3.856 ± 0.061	4.449ValVal: 4.449 ± 0.07	0.633ValTrp: 0.633 ± 0.023	2.312ValTyr: 2.312 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.633TrpAla: 0.633 ± 0.021	0.08TrpCys: 0.08 ± 0.009	0.548TrpAsp: 0.548 ± 0.025	0.728TrpGlu: 0.728 ± 0.025	0.535TrpPhe: 0.535 ± 0.022	0.725TrpGly: 0.725 ± 0.024	0.193TrpHis: 0.193 ± 0.013	0.788TrpIle: 0.788 ± 0.029	0.84TrpLys: 0.84 ± 0.031	1.144TrpLeu: 1.144 ± 0.034	0.384TrpMet: 0.384 ± 0.017	0.584TrpAsn: 0.584 ± 0.026	0.273TrpPro: 0.273 ± 0.017	0.358TrpGln: 0.358 ± 0.017	0.436TrpArg: 0.436 ± 0.021	0.673TrpSer: 0.673 ± 0.026	0.563TrpThr: 0.563 ± 0.023	0.674TrpVal: 0.674 ± 0.024	0.154TrpTrp: 0.154 ± 0.011	0.361TrpTyr: 0.361 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.892TyrAla: 1.892 ± 0.038	0.33TyrCys: 0.33 ± 0.016	1.899TyrAsp: 1.899 ± 0.054	2.675TyrGlu: 2.675 ± 0.049	1.992TyrPhe: 1.992 ± 0.044	2.464TyrGly: 2.464 ± 0.051	0.846TyrHis: 0.846 ± 0.026	2.521TyrIle: 2.521 ± 0.042	2.184TyrLys: 2.184 ± 0.047	3.524TyrLeu: 3.524 ± 0.054	0.84TyrMet: 0.84 ± 0.028	1.467TyrAsn: 1.467 ± 0.038	1.393TyrPro: 1.393 ± 0.038	1.485TyrGln: 1.485 ± 0.039	1.683TyrArg: 1.683 ± 0.042	2.29TyrSer: 2.29 ± 0.045	1.77TyrThr: 1.77 ± 0.037	1.923TyrVal: 1.923 ± 0.038	0.426TyrTrp: 0.426 ± 0.015	1.515TyrTyr: 1.515 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4589 proteins (1258239 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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