Streptococcus satellite phage Javan413
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZM60|A0A4D5ZM60_9VIRU Integrase OS=Streptococcus satellite phage Javan413 OX=2558689 GN=JavanS413_0012 PE=3 SV=1
MM1 pKa = 7.2 KK2 pKa = 9.64 TLSDD6 pKa = 3.59 TPKK9 pKa = 9.76 TFTFTTTLPNFEE21 pKa = 4.31 QAQKK25 pKa = 10.83 AGSALIGYY33 pKa = 7.54 MIGTYY38 pKa = 9.63 DD39 pKa = 3.89 QSAIDD44 pKa = 3.22 ITYY47 pKa = 9.12 TGNGNLKK54 pKa = 10.56 AEE56 pKa = 4.29 YY57 pKa = 9.75 TSDD60 pKa = 3.51 EE61 pKa = 4.37 DD62 pKa = 3.52 LTEE65 pKa = 4.1 SFEE68 pKa = 5.63 RR69 pKa = 11.84 ITNSLNHH76 pKa = 6.52 TDD78 pKa = 4.41 DD79 pKa = 4.37 FEE81 pKa = 6.99 DD82 pKa = 4.69 YY83 pKa = 11.06 EE84 pKa = 6.23 DD85 pKa = 5.59 DD86 pKa = 6.03 FEE88 pKa = 8.21 DD89 pKa = 5.79 DD90 pKa = 4.55 DD91 pKa = 4.4 LTIKK95 pKa = 10.86 GDD97 pKa = 3.82 LANYY101 pKa = 6.99 TALIGSFNTLGEE113 pKa = 4.17 AQAFTEE119 pKa = 4.58 NLDD122 pKa = 4.44 DD123 pKa = 6.72 DD124 pKa = 4.55 LTNGNNFVYY133 pKa = 9.96 EE134 pKa = 4.1 QTPDD138 pKa = 3.27 TVALYY143 pKa = 9.58 VSPQEE148 pKa = 4.29 TIANSTIQKK157 pKa = 10.38 LEE159 pKa = 3.71 EE160 pKa = 4.37 AYY162 pKa = 10.58 VKK164 pKa = 10.75 HH165 pKa = 5.76 EE166 pKa = 4.46 ADD168 pKa = 3.79 YY169 pKa = 11.46 EE170 pKa = 4.28 PDD172 pKa = 3.54 TFKK175 pKa = 11.14 LSYY178 pKa = 10.7 HH179 pKa = 6.34 DD180 pKa = 4.09 MRR182 pKa = 11.84 VQQLQSLDD190 pKa = 3.73 KK191 pKa = 10.18 DD192 pKa = 3.95 TLISDD197 pKa = 4.3 IILYY201 pKa = 9.87 EE202 pKa = 4.7 LEE204 pKa = 4.7 LLDD207 pKa = 4.53 HH208 pKa = 7.54 ADD210 pKa = 3.89 RR211 pKa = 11.84 LLSDD215 pKa = 4.22 EE216 pKa = 5.14 PLPLDD221 pKa = 4.17 DD222 pKa = 3.37 QHH224 pKa = 8.21 GFEE227 pKa = 4.67 TIEE230 pKa = 4.04 LLGDD234 pKa = 3.73 DD235 pKa = 4.96 VIDD238 pKa = 4.37 LVKK241 pKa = 10.75 EE242 pKa = 3.9 LDD244 pKa = 3.4 TSKK247 pKa = 10.86 EE248 pKa = 3.45 FDD250 pKa = 4.59 GIHH253 pKa = 7.29 DD254 pKa = 4.33 YY255 pKa = 11.0 IIGGG259 pKa = 3.65
Molecular weight: 29.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.918
IPC2_protein 3.757
IPC_protein 3.783
Toseland 3.554
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.126
Thurlkill 3.605
EMBOSS 3.706
Sillero 3.897
Patrickios 2.753
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.846
Protein with the highest isoelectric point:
>tr|A0A4D5ZM77|A0A4D5ZM77_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan413 OX=2558689 GN=JavanS413_0010 PE=4 SV=1
MM1 pKa = 7.83 KK2 pKa = 10.07 IIEE5 pKa = 4.24 YY6 pKa = 9.85 KK7 pKa = 10.36 KK8 pKa = 10.26 KK9 pKa = 10.46 NGTIMYY15 pKa = 9.39 RR16 pKa = 11.84 SQIYY20 pKa = 10.54 LGVDD24 pKa = 3.78 SITGKK29 pKa = 10.33 KK30 pKa = 10.19 ARR32 pKa = 11.84 TSVSGRR38 pKa = 11.84 TKK40 pKa = 10.78 KK41 pKa = 9.89 EE42 pKa = 3.5 LKK44 pKa = 10.87 NNIKK48 pKa = 9.59 LAKK51 pKa = 10.46 YY52 pKa = 8.03 EE53 pKa = 4.07 FQANGEE59 pKa = 4.46 TVSKK63 pKa = 10.21 KK64 pKa = 9.68 VQVKK68 pKa = 8.92 TYY70 pKa = 9.61 SQLVEE75 pKa = 4.2 LWLEE79 pKa = 4.11 NYY81 pKa = 10.23 KK82 pKa = 9.32 LTIKK86 pKa = 9.75 PQTYY90 pKa = 9.3 RR91 pKa = 11.84 VTTRR95 pKa = 11.84 LIDD98 pKa = 3.71 CHH100 pKa = 6.68 LLPYY104 pKa = 9.23 FGKK107 pKa = 9.86 MKK109 pKa = 10.23 VDD111 pKa = 4.28 KK112 pKa = 10.75 ISNTDD117 pKa = 2.69 IQSFVNGLSSKK128 pKa = 10.17 LVNFRR133 pKa = 11.84 TVNSINTRR141 pKa = 11.84 VLQYY145 pKa = 10.84 GVSLNLIPFNPARR158 pKa = 11.84 DD159 pKa = 3.53 VILPKK164 pKa = 9.97 KK165 pKa = 9.65 QKK167 pKa = 9.89 PGRR170 pKa = 11.84 DD171 pKa = 3.08 SVKK174 pKa = 10.81 FIDD177 pKa = 3.97 TNDD180 pKa = 3.44 LKK182 pKa = 10.45 TLLNFMEE189 pKa = 4.37 KK190 pKa = 10.19 QSFRR194 pKa = 11.84 RR195 pKa = 11.84 YY196 pKa = 8.64 RR197 pKa = 11.84 YY198 pKa = 7.54 QVEE201 pKa = 4.13 YY202 pKa = 11.1 VFYY205 pKa = 10.82 KK206 pKa = 10.89 LLLATGLRR214 pKa = 11.84 IGEE217 pKa = 4.13 AVALEE222 pKa = 4.13 WSDD225 pKa = 3.65 IDD227 pKa = 5.53 LEE229 pKa = 4.32 NATIDD234 pKa = 3.43 INKK237 pKa = 9.94 SYY239 pKa = 10.46 SQEE242 pKa = 3.67 LRR244 pKa = 11.84 LVSTTKK250 pKa = 10.14 TKK252 pKa = 10.93 AGTRR256 pKa = 11.84 LISIDD261 pKa = 3.8 KK262 pKa = 8.85 EE263 pKa = 4.68 TVNLLKK269 pKa = 10.53 LYY271 pKa = 8.46 QVRR274 pKa = 11.84 QRR276 pKa = 11.84 QLFHH280 pKa = 6.65 EE281 pKa = 4.75 VGGGVPKK288 pKa = 10.71 VAFATPTRR296 pKa = 11.84 EE297 pKa = 3.87 YY298 pKa = 10.93 LSRR301 pKa = 11.84 LTLQNNLNSRR311 pKa = 11.84 CEE313 pKa = 3.75 RR314 pKa = 11.84 LGIPRR319 pKa = 11.84 FTFHH323 pKa = 7.74 AFRR326 pKa = 11.84 HH327 pKa = 4.69 THH329 pKa = 6.92 ASLLLNAGISYY340 pKa = 10.68 KK341 pKa = 10.23 EE342 pKa = 3.61 LQYY345 pKa = 11.52 RR346 pKa = 11.84 LGHH349 pKa = 5.94 SNIAMTLDD357 pKa = 3.93 VYY359 pKa = 11.5 SHH361 pKa = 7.06 LSKK364 pKa = 11.04 DD365 pKa = 3.51 KK366 pKa = 10.41 EE367 pKa = 4.25 KK368 pKa = 10.93 EE369 pKa = 3.85 AVSYY373 pKa = 9.5 YY374 pKa = 10.51 EE375 pKa = 4.18 KK376 pKa = 10.61 AINSII381 pKa = 3.83
Molecular weight: 43.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.124
IPC2_protein 9.385
IPC_protein 9.326
Toseland 9.999
ProMoST 9.677
Dawson 10.204
Bjellqvist 9.867
Wikipedia 10.379
Rodwell 10.687
Grimsley 10.277
Solomon 10.218
Lehninger 10.189
Nozaki 9.984
DTASelect 9.867
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.116
Patrickios 10.131
IPC_peptide 10.218
IPC2_peptide 8.404
IPC2.peptide.svr19 8.394
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
1886
42
381
157.2
18.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.56 ± 0.347
0.636 ± 0.166
6.787 ± 1.079
8.908 ± 1.073
3.552 ± 0.304
4.454 ± 0.39
1.166 ± 0.263
8.271 ± 0.515
9.491 ± 0.735
9.862 ± 0.617
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.227 ± 0.367
6.522 ± 0.48
2.28 ± 0.373
4.03 ± 0.369
4.719 ± 0.779
5.992 ± 0.525
6.363 ± 0.751
4.242 ± 0.629
0.954 ± 0.215
4.984 ± 0.489
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here