Bagarius yarrelli (Goonch) (Bagrus yarrelli)
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17117 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A556TWN5|A0A556TWN5_BAGYA Uncharacterized protein OS=Bagarius yarrelli OX=175774 GN=Baya_5401 PE=4 SV=1
MM1 pKa = 7.09 QKK3 pKa = 8.93 CTVTSSEE10 pKa = 3.9 GTEE13 pKa = 4.22 FNALGTEE20 pKa = 4.3 SNAVSTKK27 pKa = 10.32 SNEE30 pKa = 3.85 AGTEE34 pKa = 4.2 SNAVGAVSDD43 pKa = 4.15 SVAIALKK50 pKa = 10.5 AAVSEE55 pKa = 4.67 SNAVRR60 pKa = 11.84 IEE62 pKa = 4.17 SNEE65 pKa = 3.6 VSALGTGSIADD76 pKa = 3.67 GTEE79 pKa = 3.58 YY80 pKa = 11.1 DD81 pKa = 4.09 AVGTTFNALGTEE93 pKa = 3.9 SDD95 pKa = 3.61 AFHH98 pKa = 7.3 IDD100 pKa = 3.19 SLHH103 pKa = 6.73 YY104 pKa = 10.26 PEE106 pKa = 5.45 QDD108 pKa = 2.67 NHH110 pKa = 6.56 KK111 pKa = 10.69 NQDD114 pKa = 3.12 HH115 pKa = 7.0 DD116 pKa = 4.91 QNDD119 pKa = 3.87 PNQNPNHH126 pKa = 6.93 DD127 pKa = 4.44 NYY129 pKa = 11.39 NPYY132 pKa = 10.12 QDD134 pKa = 3.79 NHH136 pKa = 6.99 YY137 pKa = 10.12 PDD139 pKa = 4.2 QDD141 pKa = 3.93 NNDD144 pKa = 3.87 PNYY147 pKa = 10.51 EE148 pKa = 4.5 DD149 pKa = 5.29 NDD151 pKa = 4.02 PDD153 pKa = 3.87 QDD155 pKa = 3.61 NHH157 pKa = 6.83 NPIYY161 pKa = 10.49 QNNDD165 pKa = 3.27 PDD167 pKa = 4.47 KK168 pKa = 11.1 DD169 pKa = 3.66 NHH171 pKa = 5.23 TSIYY175 pKa = 10.48 EE176 pKa = 4.14 NNNPNQEE183 pKa = 4.06 NHH185 pKa = 6.63 DD186 pKa = 4.78 LIYY189 pKa = 10.96 EE190 pKa = 4.28 NNDD193 pKa = 3.34 PDD195 pKa = 5.13 QDD197 pKa = 3.7 NHH199 pKa = 6.98 DD200 pKa = 4.21 PNN202 pKa = 4.37
Molecular weight: 22.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.834
IPC_protein 3.846
Toseland 3.63
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.681
Grimsley 3.541
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.202
Thurlkill 3.681
EMBOSS 3.795
Sillero 3.973
Patrickios 0.757
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.869
Protein with the highest isoelectric point:
>tr|A0A556U1E5|A0A556U1E5_BAGYA WD repeat-containing protein 90 OS=Bagarius yarrelli OX=175774 GN=Baya_6623 PE=4 SV=1
MM1 pKa = 7.48 TEE3 pKa = 4.01 TGVSHH8 pKa = 7.15 ARR10 pKa = 11.84 AKK12 pKa = 10.43 RR13 pKa = 11.84 PKK15 pKa = 9.41 TSRR18 pKa = 11.84 KK19 pKa = 8.69 KK20 pKa = 10.15 AKK22 pKa = 9.53 SHH24 pKa = 6.06 PRR26 pKa = 11.84 YY27 pKa = 10.13 SDD29 pKa = 3.49 MIRR32 pKa = 11.84 AAVLADD38 pKa = 3.2 KK39 pKa = 10.53 HH40 pKa = 6.76 RR41 pKa = 11.84 SGASRR46 pKa = 11.84 QSIQKK51 pKa = 9.37 FVKK54 pKa = 9.19 SHH56 pKa = 5.59 YY57 pKa = 9.95 KK58 pKa = 10.24 VGGNADD64 pKa = 3.69 AQIKK68 pKa = 9.8 LALKK72 pKa = 10.03 RR73 pKa = 11.84 LLANGYY79 pKa = 9.21 LRR81 pKa = 11.84 HH82 pKa = 5.84 TKK84 pKa = 10.61 GLGASGAFKK93 pKa = 10.68 LIRR96 pKa = 11.84 AGGPAGRR103 pKa = 11.84 KK104 pKa = 7.78 RR105 pKa = 11.84 KK106 pKa = 9.34 RR107 pKa = 11.84 VVRR110 pKa = 11.84 RR111 pKa = 11.84 RR112 pKa = 11.84 KK113 pKa = 9.41 PKK115 pKa = 9.58 AGRR118 pKa = 11.84 AKK120 pKa = 8.94 RR121 pKa = 11.84 TRR123 pKa = 11.84 RR124 pKa = 11.84 ARR126 pKa = 11.84 KK127 pKa = 6.54 TRR129 pKa = 11.84 KK130 pKa = 8.79 ARR132 pKa = 11.84 KK133 pKa = 5.9 TRR135 pKa = 11.84 RR136 pKa = 11.84 VRR138 pKa = 11.84 KK139 pKa = 7.03 TRR141 pKa = 11.84 RR142 pKa = 11.84 ARR144 pKa = 11.84 KK145 pKa = 6.33 TRR147 pKa = 11.84 RR148 pKa = 11.84 AAGSRR153 pKa = 11.84 KK154 pKa = 8.25 RR155 pKa = 11.84 TARR158 pKa = 11.84 KK159 pKa = 9.5 KK160 pKa = 10.45 KK161 pKa = 8.14 RR162 pKa = 11.84 TTGRR166 pKa = 11.84 RR167 pKa = 11.84 RR168 pKa = 11.84 KK169 pKa = 8.89 AAKK172 pKa = 9.48 RR173 pKa = 11.84 SKK175 pKa = 10.26 SAARR179 pKa = 11.84 PRR181 pKa = 11.84 KK182 pKa = 8.49 VKK184 pKa = 10.12 RR185 pKa = 11.84 AARR188 pKa = 11.84 RR189 pKa = 11.84 KK190 pKa = 7.3 TKK192 pKa = 10.35 ARR194 pKa = 11.84 RR195 pKa = 11.84 TGGKK199 pKa = 9.05 KK200 pKa = 9.18 AARR203 pKa = 11.84 MKK205 pKa = 10.8
Molecular weight: 23.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.111
IPC_protein 12.53
Toseland 12.705
ProMoST 13.188
Dawson 12.705
Bjellqvist 12.691
Wikipedia 13.173
Rodwell 12.471
Grimsley 12.749
Solomon 13.188
Lehninger 13.1
Nozaki 12.705
DTASelect 12.691
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.179
IPC_peptide 13.203
IPC2_peptide 12.179
IPC2.peptide.svr19 9.049
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17117
0
17117
9727499
50
11270
568.3
63.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.387 ± 0.015
2.211 ± 0.013
5.146 ± 0.013
7.046 ± 0.024
3.615 ± 0.013
5.962 ± 0.021
2.67 ± 0.01
4.538 ± 0.013
5.856 ± 0.021
9.505 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.008
4.049 ± 0.011
5.55 ± 0.023
4.747 ± 0.017
5.617 ± 0.014
8.813 ± 0.027
5.738 ± 0.015
6.352 ± 0.015
1.123 ± 0.005
2.722 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here