Amino acid dipepetide frequency for Klebsiella phage KMI6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.182AlaAla: 15.182 ± 1.364
0.922AlaCys: 0.922 ± 0.232
6.527AlaAsp: 6.527 ± 0.611
5.321AlaGlu: 5.321 ± 0.725
2.909AlaPhe: 2.909 ± 0.363
8.016AlaGly: 8.016 ± 1.123
1.206AlaHis: 1.206 ± 0.316
4.824AlaIle: 4.824 ± 0.678
4.469AlaLys: 4.469 ± 0.852
9.152AlaLeu: 9.152 ± 0.809
2.98AlaMet: 2.98 ± 0.329
3.618AlaAsn: 3.618 ± 0.417
4.327AlaPro: 4.327 ± 0.866
5.037AlaGln: 5.037 ± 0.91
5.037AlaArg: 5.037 ± 0.623
5.321AlaSer: 5.321 ± 0.596
5.179AlaThr: 5.179 ± 0.722
7.094AlaVal: 7.094 ± 0.717
1.419AlaTrp: 1.419 ± 0.352
4.044AlaTyr: 4.044 ± 0.481
0.0AlaXaa: 0.0 ± 0.0
Cys
0.993CysAla: 0.993 ± 0.345
0.426CysCys: 0.426 ± 0.186
0.709CysAsp: 0.709 ± 0.219
0.709CysGlu: 0.709 ± 0.215
0.355CysPhe: 0.355 ± 0.176
1.064CysGly: 1.064 ± 0.336
0.497CysHis: 0.497 ± 0.217
0.284CysIle: 0.284 ± 0.155
0.78CysLys: 0.78 ± 0.26
0.78CysLeu: 0.78 ± 0.247
0.638CysMet: 0.638 ± 0.192
0.709CysAsn: 0.709 ± 0.267
0.78CysPro: 0.78 ± 0.236
0.284CysGln: 0.284 ± 0.143
0.851CysArg: 0.851 ± 0.194
1.348CysSer: 1.348 ± 0.332
1.277CysThr: 1.277 ± 0.324
0.993CysVal: 0.993 ± 0.323
0.426CysTrp: 0.426 ± 0.241
0.568CysTyr: 0.568 ± 0.172
0.0CysXaa: 0.0 ± 0.0
Asp
7.236AspAla: 7.236 ± 0.889
1.135AspCys: 1.135 ± 0.303
2.838AspAsp: 2.838 ± 0.499
3.192AspGlu: 3.192 ± 0.449
2.341AspPhe: 2.341 ± 0.429
4.54AspGly: 4.54 ± 0.515
0.78AspHis: 0.78 ± 0.237
3.405AspIle: 3.405 ± 0.537
2.483AspLys: 2.483 ± 0.422
4.824AspLeu: 4.824 ± 0.541
2.412AspMet: 2.412 ± 0.395
2.838AspAsn: 2.838 ± 0.449
2.483AspPro: 2.483 ± 0.41
1.348AspGln: 1.348 ± 0.307
2.767AspArg: 2.767 ± 0.583
5.108AspSer: 5.108 ± 0.52
3.405AspThr: 3.405 ± 0.584
3.831AspVal: 3.831 ± 0.412
1.064AspTrp: 1.064 ± 0.195
2.199AspTyr: 2.199 ± 0.364
0.0AspXaa: 0.0 ± 0.0
Glu
5.463GluAla: 5.463 ± 0.748
0.851GluCys: 0.851 ± 0.238
3.192GluAsp: 3.192 ± 0.442
3.476GluGlu: 3.476 ± 0.694
2.057GluPhe: 2.057 ± 0.344
3.547GluGly: 3.547 ± 0.434
2.412GluHis: 2.412 ± 0.538
1.986GluIle: 1.986 ± 0.387
1.844GluLys: 1.844 ± 0.371
4.966GluLeu: 4.966 ± 0.58
2.27GluMet: 2.27 ± 0.375
1.49GluAsn: 1.49 ± 0.303
2.412GluPro: 2.412 ± 0.443
3.405GluGln: 3.405 ± 0.574
3.334GluArg: 3.334 ± 0.529
3.192GluSer: 3.192 ± 0.548
2.98GluThr: 2.98 ± 0.468
4.327GluVal: 4.327 ± 0.578
0.709GluTrp: 0.709 ± 0.21
3.192GluTyr: 3.192 ± 0.414
0.0GluXaa: 0.0 ± 0.0
Phe
2.696PheAla: 2.696 ± 0.331
0.497PheCys: 0.497 ± 0.216
2.483PheAsp: 2.483 ± 0.338
1.986PheGlu: 1.986 ± 0.383
1.206PhePhe: 1.206 ± 0.258
2.199PheGly: 2.199 ± 0.408
0.426PheHis: 0.426 ± 0.154
1.348PheIle: 1.348 ± 0.283
2.057PheLys: 2.057 ± 0.412
1.915PheLeu: 1.915 ± 0.349
0.568PheMet: 0.568 ± 0.246
1.348PheAsn: 1.348 ± 0.358
1.135PhePro: 1.135 ± 0.226
1.348PheGln: 1.348 ± 0.272
1.49PheArg: 1.49 ± 0.324
2.128PheSer: 2.128 ± 0.404
1.986PheThr: 1.986 ± 0.458
1.986PheVal: 1.986 ± 0.445
0.426PheTrp: 0.426 ± 0.163
1.277PheTyr: 1.277 ± 0.274
0.0PheXaa: 0.0 ± 0.0
Gly
6.598GlyAla: 6.598 ± 0.815
1.632GlyCys: 1.632 ± 0.351
4.469GlyAsp: 4.469 ± 0.519
4.044GlyGlu: 4.044 ± 0.496
2.625GlyPhe: 2.625 ± 0.408
4.611GlyGly: 4.611 ± 0.575
1.561GlyHis: 1.561 ± 0.357
3.902GlyIle: 3.902 ± 0.49
3.902GlyLys: 3.902 ± 0.569
6.243GlyLeu: 6.243 ± 0.646
1.986GlyMet: 1.986 ± 0.47
3.334GlyAsn: 3.334 ± 0.472
1.915GlyPro: 1.915 ± 0.356
2.696GlyGln: 2.696 ± 0.436
5.746GlyArg: 5.746 ± 0.52
5.604GlySer: 5.604 ± 0.661
4.824GlyThr: 4.824 ± 0.48
5.959GlyVal: 5.959 ± 0.594
0.78GlyTrp: 0.78 ± 0.223
3.618GlyTyr: 3.618 ± 0.493
0.0GlyXaa: 0.0 ± 0.0
His
1.419HisAla: 1.419 ± 0.446
0.497HisCys: 0.497 ± 0.204
1.206HisAsp: 1.206 ± 0.254
1.348HisGlu: 1.348 ± 0.375
0.284HisPhe: 0.284 ± 0.138
2.057HisGly: 2.057 ± 0.478
0.284HisHis: 0.284 ± 0.214
1.277HisIle: 1.277 ± 0.281
0.993HisLys: 0.993 ± 0.316
2.341HisLeu: 2.341 ± 0.504
0.568HisMet: 0.568 ± 0.165
0.78HisAsn: 0.78 ± 0.244
0.78HisPro: 0.78 ± 0.304
0.638HisGln: 0.638 ± 0.176
1.632HisArg: 1.632 ± 0.381
0.709HisSer: 0.709 ± 0.283
0.497HisThr: 0.497 ± 0.162
0.78HisVal: 0.78 ± 0.182
0.284HisTrp: 0.284 ± 0.155
0.922HisTyr: 0.922 ± 0.238
0.0HisXaa: 0.0 ± 0.0
Ile
3.121IleAla: 3.121 ± 0.475
0.355IleCys: 0.355 ± 0.15
2.767IleAsp: 2.767 ± 0.436
2.412IleGlu: 2.412 ± 0.418
0.568IlePhe: 0.568 ± 0.171
2.341IleGly: 2.341 ± 0.368
1.206IleHis: 1.206 ± 0.367
2.341IleIle: 2.341 ± 0.368
3.263IleLys: 3.263 ± 0.65
4.327IleLeu: 4.327 ± 0.477
1.419IleMet: 1.419 ± 0.297
1.986IleAsn: 1.986 ± 0.343
2.341IlePro: 2.341 ± 0.382
3.405IleGln: 3.405 ± 0.518
3.192IleArg: 3.192 ± 0.48
3.476IleSer: 3.476 ± 0.502
2.696IleThr: 2.696 ± 0.465
2.625IleVal: 2.625 ± 0.409
0.142IleTrp: 0.142 ± 0.102
1.277IleTyr: 1.277 ± 0.246
0.0IleXaa: 0.0 ± 0.0
Lys
5.817LysAla: 5.817 ± 0.939
0.709LysCys: 0.709 ± 0.218
2.554LysAsp: 2.554 ± 0.322
3.192LysGlu: 3.192 ± 0.562
0.922LysPhe: 0.922 ± 0.268
2.909LysGly: 2.909 ± 0.494
0.922LysHis: 0.922 ± 0.327
1.277LysIle: 1.277 ± 0.275
2.057LysLys: 2.057 ± 0.395
4.398LysLeu: 4.398 ± 0.63
1.277LysMet: 1.277 ± 0.293
1.419LysAsn: 1.419 ± 0.265
1.419LysPro: 1.419 ± 0.328
3.476LysGln: 3.476 ± 0.604
2.909LysArg: 2.909 ± 0.373
2.696LysSer: 2.696 ± 0.393
2.838LysThr: 2.838 ± 0.429
3.476LysVal: 3.476 ± 0.509
0.78LysTrp: 0.78 ± 0.217
1.703LysTyr: 1.703 ± 0.338
0.0LysXaa: 0.0 ± 0.0
Leu
8.371LeuAla: 8.371 ± 0.806
1.277LeuCys: 1.277 ± 0.301
6.172LeuAsp: 6.172 ± 0.603
6.03LeuGlu: 6.03 ± 0.492
2.625LeuPhe: 2.625 ± 0.368
6.74LeuGly: 6.74 ± 0.677
1.632LeuHis: 1.632 ± 0.383
3.902LeuIle: 3.902 ± 0.594
2.554LeuLys: 2.554 ± 0.429
6.81LeuLeu: 6.81 ± 0.639
2.057LeuMet: 2.057 ± 0.36
3.76LeuAsn: 3.76 ± 0.41
3.121LeuPro: 3.121 ± 0.462
4.257LeuGln: 4.257 ± 0.412
6.385LeuArg: 6.385 ± 0.541
5.321LeuSer: 5.321 ± 0.634
4.966LeuThr: 4.966 ± 0.631
6.385LeuVal: 6.385 ± 0.744
1.135LeuTrp: 1.135 ± 0.26
2.909LeuTyr: 2.909 ± 0.455
0.0LeuXaa: 0.0 ± 0.0
Met
3.121MetAla: 3.121 ± 0.507
0.213MetCys: 0.213 ± 0.095
2.128MetAsp: 2.128 ± 0.477
0.851MetGlu: 0.851 ± 0.221
0.993MetPhe: 0.993 ± 0.346
1.277MetGly: 1.277 ± 0.29
0.922MetHis: 0.922 ± 0.267
1.135MetIle: 1.135 ± 0.277
1.348MetLys: 1.348 ± 0.275
3.334MetLeu: 3.334 ± 0.462
0.709MetMet: 0.709 ± 0.253
1.064MetAsn: 1.064 ± 0.348
1.348MetPro: 1.348 ± 0.234
1.703MetGln: 1.703 ± 0.314
1.915MetArg: 1.915 ± 0.402
2.27MetSer: 2.27 ± 0.436
1.277MetThr: 1.277 ± 0.366
2.128MetVal: 2.128 ± 0.366
0.568MetTrp: 0.568 ± 0.16
0.922MetTyr: 0.922 ± 0.319
0.0MetXaa: 0.0 ± 0.0
Asn
3.121AsnAla: 3.121 ± 0.387
0.568AsnCys: 0.568 ± 0.196
2.057AsnAsp: 2.057 ± 0.483
1.632AsnGlu: 1.632 ± 0.276
1.419AsnPhe: 1.419 ± 0.361
4.115AsnGly: 4.115 ± 0.694
0.284AsnHis: 0.284 ± 0.149
2.483AsnIle: 2.483 ± 0.456
1.774AsnLys: 1.774 ± 0.318
3.618AsnLeu: 3.618 ± 0.47
0.993AsnMet: 0.993 ± 0.29
1.844AsnAsn: 1.844 ± 0.358
2.27AsnPro: 2.27 ± 0.43
1.632AsnGln: 1.632 ± 0.343
2.341AsnArg: 2.341 ± 0.365
3.192AsnSer: 3.192 ± 0.512
2.838AsnThr: 2.838 ± 0.389
3.547AsnVal: 3.547 ± 0.479
0.497AsnTrp: 0.497 ± 0.2
1.348AsnTyr: 1.348 ± 0.264
0.0AsnXaa: 0.0 ± 0.0
Pro
4.611ProAla: 4.611 ± 0.837
0.355ProCys: 0.355 ± 0.194
2.057ProAsp: 2.057 ± 0.454
3.334ProGlu: 3.334 ± 0.453
0.993ProPhe: 0.993 ± 0.21
3.051ProGly: 3.051 ± 0.553
0.568ProHis: 0.568 ± 0.199
1.915ProIle: 1.915 ± 0.344
1.986ProLys: 1.986 ± 0.361
2.838ProLeu: 2.838 ± 0.506
1.561ProMet: 1.561 ± 0.34
1.348ProAsn: 1.348 ± 0.302
0.993ProPro: 0.993 ± 0.321
1.348ProGln: 1.348 ± 0.281
1.703ProArg: 1.703 ± 0.318
2.412ProSer: 2.412 ± 0.35
2.554ProThr: 2.554 ± 0.358
2.909ProVal: 2.909 ± 0.39
0.638ProTrp: 0.638 ± 0.217
1.206ProTyr: 1.206 ± 0.324
0.0ProXaa: 0.0 ± 0.0
Gln
5.179GlnAla: 5.179 ± 0.677
0.426GlnCys: 0.426 ± 0.184
2.554GlnAsp: 2.554 ± 0.51
3.902GlnGlu: 3.902 ± 0.559
1.561GlnPhe: 1.561 ± 0.294
3.121GlnGly: 3.121 ± 0.6
1.348GlnHis: 1.348 ± 0.387
1.206GlnIle: 1.206 ± 0.28
2.128GlnLys: 2.128 ± 0.42
5.037GlnLeu: 5.037 ± 0.559
1.419GlnMet: 1.419 ± 0.276
2.128GlnAsn: 2.128 ± 0.358
1.703GlnPro: 1.703 ± 0.398
3.051GlnGln: 3.051 ± 0.703
3.263GlnArg: 3.263 ± 0.422
2.98GlnSer: 2.98 ± 0.382
1.986GlnThr: 1.986 ± 0.464
2.625GlnVal: 2.625 ± 0.551
0.851GlnTrp: 0.851 ± 0.226
1.632GlnTyr: 1.632 ± 0.319
0.0GlnXaa: 0.0 ± 0.0
Arg
6.314ArgAla: 6.314 ± 0.984
1.135ArgCys: 1.135 ± 0.341
3.192ArgAsp: 3.192 ± 0.503
3.476ArgGlu: 3.476 ± 0.371
1.915ArgPhe: 1.915 ± 0.313
4.611ArgGly: 4.611 ± 0.751
0.638ArgHis: 0.638 ± 0.171
3.263ArgIle: 3.263 ± 0.392
3.405ArgLys: 3.405 ± 0.604
5.179ArgLeu: 5.179 ± 0.492
1.915ArgMet: 1.915 ± 0.328
2.838ArgAsn: 2.838 ± 0.387
1.703ArgPro: 1.703 ± 0.464
2.483ArgGln: 2.483 ± 0.421
4.257ArgArg: 4.257 ± 0.631
2.554ArgSer: 2.554 ± 0.507
4.257ArgThr: 4.257 ± 0.438
4.327ArgVal: 4.327 ± 0.496
0.922ArgTrp: 0.922 ± 0.26
2.341ArgTyr: 2.341 ± 0.368
0.0ArgXaa: 0.0 ± 0.0
Ser
7.52SerAla: 7.52 ± 0.75
0.851SerCys: 0.851 ± 0.259
3.76SerAsp: 3.76 ± 0.578
2.909SerGlu: 2.909 ± 0.414
2.128SerPhe: 2.128 ± 0.463
6.527SerGly: 6.527 ± 0.878
0.851SerHis: 0.851 ± 0.242
3.334SerIle: 3.334 ± 0.586
3.334SerLys: 3.334 ± 0.557
4.895SerLeu: 4.895 ± 0.677
2.341SerMet: 2.341 ± 0.391
2.98SerAsn: 2.98 ± 0.549
2.483SerPro: 2.483 ± 0.385
2.483SerGln: 2.483 ± 0.48
3.051SerArg: 3.051 ± 0.456
4.115SerSer: 4.115 ± 0.581
3.902SerThr: 3.902 ± 0.401
4.115SerVal: 4.115 ± 0.42
0.78SerTrp: 0.78 ± 0.242
1.703SerTyr: 1.703 ± 0.391
0.0SerXaa: 0.0 ± 0.0
Thr
5.888ThrAla: 5.888 ± 0.721
0.709ThrCys: 0.709 ± 0.271
3.263ThrAsp: 3.263 ± 0.451
2.625ThrGlu: 2.625 ± 0.559
2.128ThrPhe: 2.128 ± 0.398
5.108ThrGly: 5.108 ± 0.665
0.922ThrHis: 0.922 ± 0.308
2.412ThrIle: 2.412 ± 0.4
2.625ThrLys: 2.625 ± 0.434
4.327ThrLeu: 4.327 ± 0.595
1.632ThrMet: 1.632 ± 0.443
2.554ThrAsn: 2.554 ± 0.515
1.986ThrPro: 1.986 ± 0.297
3.121ThrGln: 3.121 ± 0.449
2.625ThrArg: 2.625 ± 0.434
4.54ThrSer: 4.54 ± 0.575
3.263ThrThr: 3.263 ± 0.475
4.327ThrVal: 4.327 ± 0.887
0.78ThrTrp: 0.78 ± 0.192
2.696ThrTyr: 2.696 ± 0.475
0.0ThrXaa: 0.0 ± 0.0
Val
6.243ValAla: 6.243 ± 0.652
0.638ValCys: 0.638 ± 0.243
5.037ValAsp: 5.037 ± 0.566
3.831ValGlu: 3.831 ± 0.501
1.632ValPhe: 1.632 ± 0.356
6.74ValGly: 6.74 ± 0.758
1.844ValHis: 1.844 ± 0.37
2.412ValIle: 2.412 ± 0.433
3.051ValLys: 3.051 ± 0.384
6.314ValLeu: 6.314 ± 0.845
1.632ValMet: 1.632 ± 0.309
2.625ValAsn: 2.625 ± 0.557
3.334ValPro: 3.334 ± 0.388
3.973ValGln: 3.973 ± 0.527
4.611ValArg: 4.611 ± 0.494
3.831ValSer: 3.831 ± 0.666
3.263ValThr: 3.263 ± 0.709
5.604ValVal: 5.604 ± 0.72
0.709ValTrp: 0.709 ± 0.218
2.483ValTyr: 2.483 ± 0.322
0.0ValXaa: 0.0 ± 0.0
Trp
0.922TrpAla: 0.922 ± 0.2
0.284TrpCys: 0.284 ± 0.131
0.851TrpAsp: 0.851 ± 0.236
1.064TrpGlu: 1.064 ± 0.221
0.78TrpPhe: 0.78 ± 0.238
0.78TrpGly: 0.78 ± 0.25
0.426TrpHis: 0.426 ± 0.163
0.568TrpIle: 0.568 ± 0.198
0.709TrpLys: 0.709 ± 0.308
1.419TrpLeu: 1.419 ± 0.267
0.071TrpMet: 0.071 ± 0.16
0.993TrpAsn: 0.993 ± 0.246
0.355TrpPro: 0.355 ± 0.176
0.426TrpGln: 0.426 ± 0.18
1.064TrpArg: 1.064 ± 0.266
0.497TrpSer: 0.497 ± 0.207
0.851TrpThr: 0.851 ± 0.177
0.993TrpVal: 0.993 ± 0.214
0.284TrpTrp: 0.284 ± 0.158
0.568TrpTyr: 0.568 ± 0.209
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.767TyrAla: 2.767 ± 0.433
0.993TyrCys: 0.993 ± 0.289
2.412TyrAsp: 2.412 ± 0.399
1.774TyrGlu: 1.774 ± 0.426
1.064TyrPhe: 1.064 ± 0.275
2.696TyrGly: 2.696 ± 0.455
0.568TyrHis: 0.568 ± 0.214
1.986TyrIle: 1.986 ± 0.429
1.986TyrLys: 1.986 ± 0.391
3.689TyrLeu: 3.689 ± 0.518
0.709TyrMet: 0.709 ± 0.29
1.915TyrAsn: 1.915 ± 0.391
1.49TyrPro: 1.49 ± 0.324
1.986TyrGln: 1.986 ± 0.346
2.554TyrArg: 2.554 ± 0.445
2.767TyrSer: 2.767 ± 0.386
2.696TyrThr: 2.696 ± 0.435
1.774TyrVal: 1.774 ± 0.386
0.78TyrTrp: 0.78 ± 0.236
1.419TyrTyr: 1.419 ± 0.311
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (14097 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski