Erwinia phage phiEaH2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Erskinevirus; Erwinia virus EaH2

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 262 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7KEA5|J7KEA5_9CAUD Uncharacterized protein OS=Erwinia phage phiEaH2 OX=1029988 PE=4 SV=1
MM1 pKa = 7.57IDD3 pKa = 3.54IYY5 pKa = 10.96TDD7 pKa = 3.37YY8 pKa = 11.55AAVLTVNRR16 pKa = 11.84HH17 pKa = 4.88EE18 pKa = 5.16DD19 pKa = 3.08RR20 pKa = 11.84AAPMLDD26 pKa = 3.7LVTLGIDD33 pKa = 3.21YY34 pKa = 10.23GYY36 pKa = 11.05DD37 pKa = 3.14VALSDD42 pKa = 4.64VYY44 pKa = 10.73FEE46 pKa = 5.03PSSDD50 pKa = 3.71PADD53 pKa = 3.33EE54 pKa = 4.55TIRR57 pKa = 11.84LEE59 pKa = 4.49GIIVKK64 pKa = 10.21CAVALGNRR72 pKa = 11.84LGVALNPQIVYY83 pKa = 9.52HH84 pKa = 6.3KK85 pKa = 9.72PKK87 pKa = 7.9EE88 pKa = 4.32TVRR91 pKa = 11.84ILHH94 pKa = 6.12GLLEE98 pKa = 4.34KK99 pKa = 10.98FEE101 pKa = 5.64DD102 pKa = 4.45FDD104 pKa = 7.03DD105 pKa = 4.35SDD107 pKa = 3.84SLYY110 pKa = 10.66GIVMSGEE117 pKa = 4.21TPEE120 pKa = 5.68HH121 pKa = 6.6ILEE124 pKa = 4.22NMCRR128 pKa = 11.84VVYY131 pKa = 10.66GDD133 pKa = 3.87NNLHH137 pKa = 6.75FEE139 pKa = 4.49DD140 pKa = 4.86LVISVGPRR148 pKa = 11.84VMTVMRR154 pKa = 11.84NYY156 pKa = 10.11LAAAALEE163 pKa = 4.18EE164 pKa = 4.19QKK166 pKa = 11.38GDD168 pKa = 3.36NDD170 pKa = 3.51YY171 pKa = 11.63LRR173 pKa = 11.84IGRR176 pKa = 11.84IAAYY180 pKa = 10.46LRR182 pKa = 11.84TFPQNPSAFVFLNLPQEE199 pKa = 4.08PDD201 pKa = 3.25MLVVQQALSFADD213 pKa = 4.77DD214 pKa = 4.5EE215 pKa = 4.82YY216 pKa = 11.85SDD218 pKa = 5.83DD219 pKa = 4.59EE220 pKa = 4.7LLLMYY225 pKa = 10.68AVGLSVIPFDD235 pKa = 5.57DD236 pKa = 3.62FDD238 pKa = 3.93GAYY241 pKa = 10.08GALAGNLEE249 pKa = 4.94KK250 pKa = 10.61INTDD254 pKa = 3.17NYY256 pKa = 9.84PPLPILEE263 pKa = 4.26QGLAALKK270 pKa = 9.54TIYY273 pKa = 10.53GQDD276 pKa = 3.14RR277 pKa = 11.84AQEE280 pKa = 4.11EE281 pKa = 4.29EE282 pKa = 4.77NYY284 pKa = 10.37EE285 pKa = 3.81QDD287 pKa = 3.06

Molecular weight: 32.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7KJE7|J7KJE7_9CAUD Putative virion structural protein OS=Erwinia phage phiEaH2 OX=1029988 PE=4 SV=1
MM1 pKa = 7.83AKK3 pKa = 10.16RR4 pKa = 11.84LRR6 pKa = 11.84ITFLEE11 pKa = 4.22NSLSIYY17 pKa = 9.63QGKK20 pKa = 8.97KK21 pKa = 9.94RR22 pKa = 11.84IYY24 pKa = 9.48HH25 pKa = 5.67EE26 pKa = 4.04PFVPVEE32 pKa = 4.19FFAGRR37 pKa = 11.84ITGVDD42 pKa = 2.89KK43 pKa = 10.63RR44 pKa = 11.84TTHH47 pKa = 5.24LQKK50 pKa = 10.52VACGAIARR58 pKa = 11.84TLRR61 pKa = 11.84EE62 pKa = 3.76RR63 pKa = 11.84QLWALHH69 pKa = 4.99QWWKK73 pKa = 9.32NAYY76 pKa = 7.4RR77 pKa = 11.84TARR80 pKa = 11.84PACLDD85 pKa = 3.07WTPRR89 pKa = 11.84VHH91 pKa = 7.43FNLLPVMTKK100 pKa = 9.6IDD102 pKa = 3.53IKK104 pKa = 11.03DD105 pKa = 3.87LNEE108 pKa = 3.95MLTVRR113 pKa = 11.84PP114 pKa = 4.08

Molecular weight: 13.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

262

0

262

74178

37

2382

283.1

31.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.982 ± 0.145

0.833 ± 0.047

6.404 ± 0.123

6.228 ± 0.159

4.067 ± 0.08

6.799 ± 0.166

2.052 ± 0.083

5.34 ± 0.085

5.437 ± 0.128

9.097 ± 0.115

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.731 ± 0.07

4.997 ± 0.108

4.353 ± 0.096

3.67 ± 0.089

5.418 ± 0.128

5.638 ± 0.102

6.417 ± 0.191

7.199 ± 0.109

1.482 ± 0.055

3.857 ± 0.094
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski