Tepidanaerobacter acetatoxydans (strain DSM 21804 / JCM 16047 / Re1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermosediminibacterales; Tepidanaerobacteraceae; Tepidanaerobacter; Tepidanaerobacter acetatoxydans

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2640 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4LTX6|F4LTX6_TEPAE 3-phosphoshikimate 1-carboxyvinyltransferase OS=Tepidanaerobacter acetatoxydans (strain DSM 21804 / JCM 16047 / Re1) OX=1209989 GN=aroA PE=3 SV=1
MM1 pKa = 7.38NNLFKK6 pKa = 11.37DD7 pKa = 3.28MLAEE11 pKa = 4.11HH12 pKa = 7.09FEE14 pKa = 3.93QEE16 pKa = 4.59IYY18 pKa = 11.13DD19 pKa = 3.91FLQDD23 pKa = 4.75HH24 pKa = 6.84IMNNYY29 pKa = 9.03DD30 pKa = 3.33QYY32 pKa = 11.98DD33 pKa = 3.61LTRR36 pKa = 11.84RR37 pKa = 11.84ANEE40 pKa = 3.74VNEE43 pKa = 3.95VLEE46 pKa = 4.41ASLDD50 pKa = 3.68LAEE53 pKa = 4.32VLRR56 pKa = 11.84VYY58 pKa = 10.55NIKK61 pKa = 10.27QDD63 pKa = 3.54GSDD66 pKa = 3.15VAFNMLVSCDD76 pKa = 3.88IEE78 pKa = 5.41IGDD81 pKa = 3.96YY82 pKa = 10.48FAGEE86 pKa = 4.53NITEE90 pKa = 4.72SVCQWFEE97 pKa = 4.02LSCSAVLEE105 pKa = 4.37NATLTDD111 pKa = 4.1FSIDD115 pKa = 4.0GIEE118 pKa = 4.73AYY120 pKa = 10.65NKK122 pKa = 10.33

Molecular weight: 14.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4LVB3|F4LVB3_TEPAE t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE OS=Tepidanaerobacter acetatoxydans (strain DSM 21804 / JCM 16047 / Re1) OX=1209989 GN=TEPIRE1_0539 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.5QPKK8 pKa = 8.62RR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 9.46RR12 pKa = 11.84KK13 pKa = 7.7RR14 pKa = 11.84THH16 pKa = 6.46GFRR19 pKa = 11.84QRR21 pKa = 11.84MLKK24 pKa = 10.26KK25 pKa = 10.21SGRR28 pKa = 11.84NVIKK32 pKa = 10.48RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.22GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2640

0

2640

799649

26

2070

302.9

33.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.145 ± 0.056

1.124 ± 0.02

5.565 ± 0.035

7.234 ± 0.046

4.092 ± 0.032

6.932 ± 0.047

1.518 ± 0.02

9.286 ± 0.044

7.897 ± 0.043

9.336 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.023

4.745 ± 0.036

3.609 ± 0.032

2.919 ± 0.028

3.985 ± 0.035

5.763 ± 0.035

5.005 ± 0.037

6.795 ± 0.04

0.721 ± 0.014

3.555 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski