Sphingobacterium deserti
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3969 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B8T3A5|A0A0B8T3A5_9SPHI RND efflux system outer membrane lipoprotein NodT family OS=Sphingobacterium deserti OX=1229276 GN=DI53_0112 PE=3 SV=1
MM1 pKa = 7.76 IDD3 pKa = 4.42 DD4 pKa = 4.28 KK5 pKa = 11.62 LGLKK9 pKa = 9.39 SWSEE13 pKa = 4.13 DD14 pKa = 3.4 PAFLAQLYY22 pKa = 10.72 SFAQANGSGSFYY34 pKa = 10.68 AIWNDD39 pKa = 3.38 GTAKK43 pKa = 10.26 PMSEE47 pKa = 3.87 MPIVVFGDD55 pKa = 3.18 EE56 pKa = 4.41 GGVHH60 pKa = 6.15 IVAEE64 pKa = 4.18 NFVQLLHH71 pKa = 6.98 LLTFDD76 pKa = 3.83 TEE78 pKa = 3.96 IHH80 pKa = 6.06 VDD82 pKa = 3.98 FDD84 pKa = 3.55 GVYY87 pKa = 9.92 FYY89 pKa = 11.17 KK90 pKa = 10.81 DD91 pKa = 3.16 EE92 pKa = 4.16 EE93 pKa = 4.6 DD94 pKa = 4.01 YY95 pKa = 11.63 EE96 pKa = 4.21 EE97 pKa = 5.95 SEE99 pKa = 4.46 DD100 pKa = 5.1 LEE102 pKa = 5.01 EE103 pKa = 4.19 FLDD106 pKa = 4.01 WVKK109 pKa = 11.12 EE110 pKa = 3.99 NYY112 pKa = 10.41 GLDD115 pKa = 3.62 QIEE118 pKa = 4.59 EE119 pKa = 4.28 PDD121 pKa = 3.86 EE122 pKa = 4.22 LMEE125 pKa = 4.5 AAQSKK130 pKa = 8.08 YY131 pKa = 8.0 QAVFEE136 pKa = 4.7 DD137 pKa = 3.65 WFGQYY142 pKa = 10.8 YY143 pKa = 10.32 SDD145 pKa = 4.64 EE146 pKa = 4.29 EE147 pKa = 4.2
Molecular weight: 17.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.681
IPC_protein 3.656
Toseland 3.465
ProMoST 3.795
Dawson 3.643
Bjellqvist 3.834
Wikipedia 3.554
Rodwell 3.49
Grimsley 3.376
Solomon 3.63
Lehninger 3.579
Nozaki 3.757
DTASelect 3.935
Thurlkill 3.503
EMBOSS 3.567
Sillero 3.783
Patrickios 0.693
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|A0A0B8T0Q4|A0A0B8T0Q4_9SPHI t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE OS=Sphingobacterium deserti OX=1229276 GN=DI53_2092 PE=3 SV=1
MM1 pKa = 8.11 DD2 pKa = 5.82 SICVRR7 pKa = 11.84 FVHH10 pKa = 5.86 RR11 pKa = 11.84 TSLPSFARR19 pKa = 11.84 TGRR22 pKa = 11.84 YY23 pKa = 6.91 FWRR26 pKa = 11.84 AKK28 pKa = 7.27 VTKK31 pKa = 9.1 TPSLEE36 pKa = 4.16 TFAGRR41 pKa = 11.84 ACAEE45 pKa = 3.68 ASFYY49 pKa = 10.54 ISQSFRR55 pKa = 11.84 GDD57 pKa = 2.6 ISFNNGPCNHH67 pKa = 7.17 LSTKK71 pKa = 10.02 TFLNTKK77 pKa = 9.36 YY78 pKa = 10.96 YY79 pKa = 11.07 NLFSLRR85 pKa = 11.84 TTNRR89 pKa = 11.84 ANDD92 pKa = 3.68 RR93 pKa = 11.84 PDD95 pKa = 3.2 LQKK98 pKa = 9.95 TINGTFTFHH107 pKa = 6.38 QAMEE111 pKa = 4.13 KK112 pKa = 10.34 RR113 pKa = 11.84 SAAVAA118 pKa = 3.55
Molecular weight: 13.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.56
IPC_protein 10.028
Toseland 10.306
ProMoST 9.999
Dawson 10.467
Bjellqvist 10.175
Wikipedia 10.657
Rodwell 10.774
Grimsley 10.54
Solomon 10.526
Lehninger 10.496
Nozaki 10.335
DTASelect 10.16
Thurlkill 10.335
EMBOSS 10.701
Sillero 10.394
Patrickios 10.496
IPC_peptide 10.526
IPC2_peptide 9.268
IPC2.peptide.svr19 8.497
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3969
0
3969
1348934
29
2269
339.9
38.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.648 ± 0.033
0.733 ± 0.01
5.619 ± 0.026
6.016 ± 0.039
4.941 ± 0.027
6.753 ± 0.035
1.951 ± 0.018
6.932 ± 0.036
6.334 ± 0.045
9.653 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.311 ± 0.017
5.29 ± 0.036
3.616 ± 0.018
3.92 ± 0.02
4.513 ± 0.025
6.489 ± 0.031
5.455 ± 0.027
6.512 ± 0.029
1.214 ± 0.016
4.099 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here