Avian gyrovirus 2
Average proteome isoelectric point is 8.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F4ZDR1|F4ZDR1_9VIRU Apoptin OS=Avian gyrovirus 2 OX=1002273 PE=3 SV=1
MM1 pKa = 7.59 SSGGLGDD8 pKa = 4.8 CSACEE13 pKa = 3.67 TRR15 pKa = 11.84 AAGGSEE21 pKa = 3.92 LPLRR25 pKa = 11.84 QEE27 pKa = 4.35 GQLGPSGAGSTGKK40 pKa = 7.95 TLNADD45 pKa = 3.69 PPLSPTSNNYY55 pKa = 8.18 SVRR58 pKa = 11.84 TINGIRR64 pKa = 11.84 ASNKK68 pKa = 8.95 FVSASRR74 pKa = 11.84 NDD76 pKa = 3.88 LDD78 pKa = 5.91 RR79 pKa = 11.84 DD80 pKa = 4.07 PNWYY84 pKa = 9.29 RR85 pKa = 11.84 VNYY88 pKa = 9.29 NYY90 pKa = 10.68 SIATWLRR97 pKa = 11.84 QCARR101 pKa = 11.84 SHH103 pKa = 7.67 DD104 pKa = 4.46 EE105 pKa = 3.53 ICTCGRR111 pKa = 11.84 WRR113 pKa = 11.84 SHH115 pKa = 5.33 WFQEE119 pKa = 3.82 ASGLVTQEE127 pKa = 3.95 TQTDD131 pKa = 3.54 QLARR135 pKa = 11.84 DD136 pKa = 3.72 LRR138 pKa = 11.84 RR139 pKa = 11.84 LQRR142 pKa = 11.84 KK143 pKa = 9.41 GEE145 pKa = 3.89 AAKK148 pKa = 10.59 RR149 pKa = 11.84 KK150 pKa = 9.51 LDD152 pKa = 3.5 YY153 pKa = 10.64 LKK155 pKa = 10.87 RR156 pKa = 11.84 RR157 pKa = 11.84 GTPHH161 pKa = 7.49 KK162 pKa = 9.42 KK163 pKa = 8.53 TKK165 pKa = 9.22 TVTWQDD171 pKa = 3.18 YY172 pKa = 11.46 DD173 pKa = 5.45 DD174 pKa = 6.12 DD175 pKa = 5.46 DD176 pKa = 5.81 LVDD179 pKa = 4.52 ALASTTEE186 pKa = 4.14 DD187 pKa = 4.28 DD188 pKa = 3.91 GTGDD192 pKa = 3.6 TDD194 pKa = 4.13 CDD196 pKa = 3.21 EE197 pKa = 4.65 DD198 pKa = 5.2 AIPGGVNFDD207 pKa = 3.83 MRR209 pKa = 11.84 VEE211 pKa = 4.51 DD212 pKa = 4.34 PLIAALRR219 pKa = 11.84 GGSSTHH225 pKa = 5.48 TRR227 pKa = 11.84 APMWW231 pKa = 4.0
Molecular weight: 25.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.605
IPC2_protein 5.626
IPC_protein 5.614
Toseland 5.817
ProMoST 5.906
Dawson 5.804
Bjellqvist 5.842
Wikipedia 5.792
Rodwell 5.766
Grimsley 5.881
Solomon 5.804
Lehninger 5.792
Nozaki 6.033
DTASelect 6.237
Thurlkill 6.148
EMBOSS 6.135
Sillero 6.122
Patrickios 4.304
IPC_peptide 5.817
IPC2_peptide 6.122
IPC2.peptide.svr19 6.15
Protein with the highest isoelectric point:
>tr|F4ZDR2|F4ZDR2_9VIRU CA1 OS=Avian gyrovirus 2 OX=1002273 GN=VP1 PE=3 SV=1
MM1 pKa = 6.13 QTPRR5 pKa = 11.84 SRR7 pKa = 11.84 RR8 pKa = 11.84 QATTTRR14 pKa = 11.84 SEE16 pKa = 3.99 LLTAYY21 pKa = 7.49 EE22 pKa = 5.27 HH23 pKa = 6.65 PTSSCPPAEE32 pKa = 4.21 TTSIEE37 pKa = 3.89 IQIGIGSTIITLSLPGYY54 pKa = 9.93 ASVRR58 pKa = 11.84 VLTTRR63 pKa = 11.84 SAPADD68 pKa = 3.91 DD69 pKa = 4.62 GGVTGSRR76 pKa = 11.84 RR77 pKa = 11.84 LADD80 pKa = 3.39 SSHH83 pKa = 5.87 RR84 pKa = 11.84 RR85 pKa = 11.84 PRR87 pKa = 11.84 RR88 pKa = 11.84 TSSPEE93 pKa = 3.4 IYY95 pKa = 10.42 VGFSAKK101 pKa = 8.49 EE102 pKa = 3.68 RR103 pKa = 11.84 RR104 pKa = 11.84 QKK106 pKa = 11.19 EE107 pKa = 3.7 NLITLRR113 pKa = 11.84 EE114 pKa = 4.1 EE115 pKa = 4.39 GPPIKK120 pKa = 10.26 KK121 pKa = 9.87 LRR123 pKa = 11.84 LL124 pKa = 3.44
Molecular weight: 13.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.619
IPC_protein 10.643
Toseland 10.687
ProMoST 10.526
Dawson 10.774
Bjellqvist 10.555
Wikipedia 11.052
Rodwell 10.818
Grimsley 10.833
Solomon 10.95
Lehninger 10.906
Nozaki 10.672
DTASelect 10.555
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.716
Patrickios 10.57
IPC_peptide 10.965
IPC2_peptide 9.663
IPC2.peptide.svr19 8.667
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
815
124
460
271.7
30.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.607 ± 0.345
1.718 ± 0.374
5.031 ± 1.964
3.558 ± 1.243
3.313 ± 1.321
7.607 ± 0.601
2.209 ± 0.313
3.313 ± 1.082
3.926 ± 0.21
7.239 ± 0.395
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.331 ± 0.734
3.681 ± 0.785
7.485 ± 1.299
5.031 ± 0.839
9.939 ± 0.81
7.362 ± 1.56
7.853 ± 1.406
4.54 ± 0.7
2.945 ± 0.927
3.313 ± 0.47
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here