Lachnoclostridium sp. An76

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium; unclassified Lachnoclostridium

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2953 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y3TFA0|A0A1Y3TFA0_9FIRM Uncharacterized protein OS=Lachnoclostridium sp. An76 OX=1965654 GN=B5G27_11630 PE=4 SV=1
MM1 pKa = 7.89IGIAILAAVIIIAVFMMQQGRR22 pKa = 11.84RR23 pKa = 11.84SDD25 pKa = 3.39EE26 pKa = 4.28DD27 pKa = 3.31NRR29 pKa = 11.84EE30 pKa = 3.92SSLGNGIYY38 pKa = 9.86IVSSEE43 pKa = 4.55PYY45 pKa = 9.26TGPFWEE51 pKa = 5.73DD52 pKa = 2.9GSDD55 pKa = 3.98AAVEE59 pKa = 4.98DD60 pKa = 3.56IWSLKK65 pKa = 9.53IVNSSDD71 pKa = 3.26EE72 pKa = 4.7DD73 pKa = 3.48IEE75 pKa = 4.17YY76 pKa = 11.0LKK78 pKa = 10.49IHH80 pKa = 6.87AATAEE85 pKa = 4.03AEE87 pKa = 4.62GNFEE91 pKa = 4.31VTVLPAGGEE100 pKa = 4.27LIVLEE105 pKa = 4.7SSAAAYY111 pKa = 8.25PQDD114 pKa = 4.82AEE116 pKa = 4.24DD117 pKa = 3.72ARR119 pKa = 11.84YY120 pKa = 9.45DD121 pKa = 3.79AEE123 pKa = 4.05NLAFFQEE130 pKa = 4.31EE131 pKa = 4.25RR132 pKa = 11.84SILPEE137 pKa = 3.61QLTLYY142 pKa = 10.49GQDD145 pKa = 2.79NWIKK149 pKa = 10.78AEE151 pKa = 4.34NISGEE156 pKa = 4.44DD157 pKa = 3.21IAGDD161 pKa = 3.04ICVYY165 pKa = 10.15YY166 pKa = 10.85KK167 pKa = 10.75NLEE170 pKa = 4.26DD171 pKa = 5.83GIFQGGITYY180 pKa = 10.37RR181 pKa = 11.84AVFPGGMAAGEE192 pKa = 4.18AEE194 pKa = 4.39EE195 pKa = 4.77QEE197 pKa = 4.48VLHH200 pKa = 5.7YY201 pKa = 10.44MKK203 pKa = 10.07DD204 pKa = 3.29TSQIMYY210 pKa = 8.17VTYY213 pKa = 10.44DD214 pKa = 3.22QEE216 pKa = 4.02

Molecular weight: 23.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y3TQF9|A0A1Y3TQF9_9FIRM Uncharacterized protein OS=Lachnoclostridium sp. An76 OX=1965654 GN=B5G27_06400 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2953

0

2953

975717

23

3393

330.4

36.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.992 ± 0.049

1.436 ± 0.02

5.994 ± 0.044

8.105 ± 0.049

3.901 ± 0.03

7.689 ± 0.048

1.675 ± 0.019

6.72 ± 0.046

6.148 ± 0.041

8.753 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.008 ± 0.024

3.793 ± 0.038

3.425 ± 0.028

3.156 ± 0.027

5.219 ± 0.051

5.624 ± 0.033

5.402 ± 0.04

6.861 ± 0.042

0.945 ± 0.018

4.155 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski