Amino acid dipepetide frequency for Siphonobacter sp. BAB-5385

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.488AlaAla: 6.488 ± 0.083	0.702AlaCys: 0.702 ± 0.022	4.151AlaAsp: 4.151 ± 0.05	5.001AlaGlu: 5.001 ± 0.062	3.719AlaPhe: 3.719 ± 0.047	5.785AlaGly: 5.785 ± 0.071	1.267AlaHis: 1.267 ± 0.028	4.496AlaIle: 4.496 ± 0.065	3.858AlaLys: 3.858 ± 0.057	7.87AlaLeu: 7.87 ± 0.085	1.692AlaMet: 1.692 ± 0.036	3.289AlaAsn: 3.289 ± 0.052	2.619AlaPro: 2.619 ± 0.039	3.78AlaGln: 3.78 ± 0.054	3.626AlaArg: 3.626 ± 0.049	5.029AlaSer: 5.029 ± 0.061	4.691AlaThr: 4.691 ± 0.066	5.004AlaVal: 5.004 ± 0.066	1.056AlaTrp: 1.056 ± 0.027	3.315AlaTyr: 3.315 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	0.334CysAla: 0.334 ± 0.016	0.095CysCys: 0.095 ± 0.008	0.327CysAsp: 0.327 ± 0.015	0.388CysGlu: 0.388 ± 0.014	0.35CysPhe: 0.35 ± 0.014	0.562CysGly: 0.562 ± 0.022	0.185CysHis: 0.185 ± 0.012	0.438CysIle: 0.438 ± 0.014	0.328CysLys: 0.328 ± 0.015	0.797CysLeu: 0.797 ± 0.023	0.176CysMet: 0.176 ± 0.011	0.256CysAsn: 0.256 ± 0.011	0.324CysPro: 0.324 ± 0.015	0.373CysGln: 0.373 ± 0.016	0.376CysArg: 0.376 ± 0.017	0.48CysSer: 0.48 ± 0.018	0.463CysThr: 0.463 ± 0.018	0.438CysVal: 0.438 ± 0.017	0.118CysTrp: 0.118 ± 0.008	0.266CysTyr: 0.266 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	3.829AspAla: 3.829 ± 0.051	0.328AspCys: 0.328 ± 0.015	2.16AspAsp: 2.16 ± 0.036	3.193AspGlu: 3.193 ± 0.045	2.844AspPhe: 2.844 ± 0.035	3.535AspGly: 3.535 ± 0.057	0.959AspHis: 0.959 ± 0.024	2.685AspIle: 2.685 ± 0.047	2.385AspLys: 2.385 ± 0.042	5.277AspLeu: 5.277 ± 0.062	0.884AspMet: 0.884 ± 0.024	1.849AspAsn: 1.849 ± 0.033	2.342AspPro: 2.342 ± 0.037	2.339AspGln: 2.339 ± 0.037	2.81AspArg: 2.81 ± 0.041	2.813AspSer: 2.813 ± 0.049	2.632AspThr: 2.632 ± 0.041	3.28AspVal: 3.28 ± 0.045	0.93AspTrp: 0.93 ± 0.024	2.231AspTyr: 2.231 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	5.259GluAla: 5.259 ± 0.065	0.302GluCys: 0.302 ± 0.014	2.629GluAsp: 2.629 ± 0.04	4.014GluGlu: 4.014 ± 0.067	2.306GluPhe: 2.306 ± 0.037	3.756GluGly: 3.756 ± 0.044	1.157GluHis: 1.157 ± 0.031	3.619GluIle: 3.619 ± 0.051	4.232GluLys: 4.232 ± 0.056	6.035GluLeu: 6.035 ± 0.07	1.396GluMet: 1.396 ± 0.029	2.801GluAsn: 2.801 ± 0.039	2.06GluPro: 2.06 ± 0.04	2.947GluGln: 2.947 ± 0.051	3.356GluArg: 3.356 ± 0.054	3.235GluSer: 3.235 ± 0.044	3.353GluThr: 3.353 ± 0.046	4.227GluVal: 4.227 ± 0.048	0.802GluTrp: 0.802 ± 0.022	2.09GluTyr: 2.09 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	3.227PheAla: 3.227 ± 0.042	0.44PheCys: 0.44 ± 0.015	2.631PheAsp: 2.631 ± 0.036	2.588PheGlu: 2.588 ± 0.043	2.276PhePhe: 2.276 ± 0.036	3.215PheGly: 3.215 ± 0.044	0.911PheHis: 0.911 ± 0.029	2.595PheIle: 2.595 ± 0.044	2.315PheLys: 2.315 ± 0.042	4.538PheLeu: 4.538 ± 0.062	0.95PheMet: 0.95 ± 0.024	2.201PheAsn: 2.201 ± 0.039	1.905PhePro: 1.905 ± 0.034	1.992PheGln: 1.992 ± 0.036	2.505PheArg: 2.505 ± 0.041	3.49PheSer: 3.49 ± 0.049	3.05PheThr: 3.05 ± 0.045	2.977PheVal: 2.977 ± 0.046	0.729PheTrp: 0.729 ± 0.023	1.889PheTyr: 1.889 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	4.39GlyAla: 4.39 ± 0.067	0.576GlyCys: 0.576 ± 0.019	3.097GlyAsp: 3.097 ± 0.047	3.517GlyGlu: 3.517 ± 0.051	3.535GlyPhe: 3.535 ± 0.052	4.884GlyGly: 4.884 ± 0.078	1.266GlyHis: 1.266 ± 0.03	4.622GlyIle: 4.622 ± 0.048	4.668GlyLys: 4.668 ± 0.06	7.142GlyLeu: 7.142 ± 0.075	1.633GlyMet: 1.633 ± 0.033	3.241GlyAsn: 3.241 ± 0.057	1.956GlyPro: 1.956 ± 0.037	2.985GlyGln: 2.985 ± 0.043	3.258GlyArg: 3.258 ± 0.051	4.544GlySer: 4.544 ± 0.067	4.758GlyThr: 4.758 ± 0.065	4.847GlyVal: 4.847 ± 0.061	1.239GlyTrp: 1.239 ± 0.025	3.052GlyTyr: 3.052 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	1.266HisAla: 1.266 ± 0.029	0.191HisCys: 0.191 ± 0.009	0.854HisAsp: 0.854 ± 0.026	1.108HisGlu: 1.108 ± 0.024	1.172HisPhe: 1.172 ± 0.029	1.159HisGly: 1.159 ± 0.028	0.514HisHis: 0.514 ± 0.019	1.018HisIle: 1.018 ± 0.024	0.753HisLys: 0.753 ± 0.022	2.185HisLeu: 2.185 ± 0.043	0.315HisMet: 0.315 ± 0.013	0.658HisAsn: 0.658 ± 0.022	1.198HisPro: 1.198 ± 0.029	0.993HisGln: 0.993 ± 0.027	1.101HisArg: 1.101 ± 0.027	1.118HisSer: 1.118 ± 0.028	1.229HisThr: 1.229 ± 0.027	1.157HisVal: 1.157 ± 0.025	0.394HisTrp: 0.394 ± 0.017	0.903HisTyr: 0.903 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	4.548IleAla: 4.548 ± 0.052	0.506IleCys: 0.506 ± 0.016	3.247IleAsp: 3.247 ± 0.043	3.561IleGlu: 3.561 ± 0.052	2.311IlePhe: 2.311 ± 0.041	4.158IleGly: 4.158 ± 0.059	1.287IleHis: 1.287 ± 0.028	3.113IleIle: 3.113 ± 0.051	2.803IleLys: 2.803 ± 0.045	5.434IleLeu: 5.434 ± 0.059	0.929IleMet: 0.929 ± 0.025	2.66IleAsn: 2.66 ± 0.041	3.074IlePro: 3.074 ± 0.041	2.836IleGln: 2.836 ± 0.036	3.684IleArg: 3.684 ± 0.048	3.797IleSer: 3.797 ± 0.056	3.75IleThr: 3.75 ± 0.046	3.519IleVal: 3.519 ± 0.054	0.686IleTrp: 0.686 ± 0.02	1.966IleTyr: 1.966 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	4.919LysAla: 4.919 ± 0.058	0.225LysCys: 0.225 ± 0.013	2.956LysAsp: 2.956 ± 0.045	3.592LysGlu: 3.592 ± 0.049	1.81LysPhe: 1.81 ± 0.031	3.866LysGly: 3.866 ± 0.055	0.96LysHis: 0.96 ± 0.025	3.306LysIle: 3.306 ± 0.045	3.684LysLys: 3.684 ± 0.052	4.867LysLeu: 4.867 ± 0.051	1.317LysMet: 1.317 ± 0.029	2.756LysAsn: 2.756 ± 0.043	2.809LysPro: 2.809 ± 0.042	2.351LysGln: 2.351 ± 0.042	2.77LysArg: 2.77 ± 0.046	3.033LysSer: 3.033 ± 0.04	3.435LysThr: 3.435 ± 0.043	3.744LysVal: 3.744 ± 0.049	0.618LysTrp: 0.618 ± 0.018	1.999LysTyr: 1.999 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	8.107LeuAla: 8.107 ± 0.087	0.742LeuCys: 0.742 ± 0.022	4.908LeuAsp: 4.908 ± 0.053	5.764LeuGlu: 5.764 ± 0.082	4.66LeuPhe: 4.66 ± 0.063	6.719LeuGly: 6.719 ± 0.076	1.95LeuHis: 1.95 ± 0.034	5.877LeuIle: 5.877 ± 0.062	5.977LeuLys: 5.977 ± 0.062	10.761LeuLeu: 10.761 ± 0.123	2.209LeuMet: 2.209 ± 0.036	4.781LeuAsn: 4.781 ± 0.06	4.922LeuPro: 4.922 ± 0.045	4.519LeuGln: 4.519 ± 0.063	5.298LeuArg: 5.298 ± 0.06	7.274LeuSer: 7.274 ± 0.08	6.749LeuThr: 6.749 ± 0.072	6.55LeuVal: 6.55 ± 0.078	1.237LeuTrp: 1.237 ± 0.031	3.597LeuTyr: 3.597 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	1.871MetAla: 1.871 ± 0.037	0.092MetCys: 0.092 ± 0.008	1.11MetAsp: 1.11 ± 0.029	1.167MetGlu: 1.167 ± 0.028	0.624MetPhe: 0.624 ± 0.019	1.496MetGly: 1.496 ± 0.034	0.442MetHis: 0.442 ± 0.017	1.209MetIle: 1.209 ± 0.029	1.665MetLys: 1.665 ± 0.034	2.017MetLeu: 2.017 ± 0.036	0.494MetMet: 0.494 ± 0.017	1.169MetAsn: 1.169 ± 0.025	1.091MetPro: 1.091 ± 0.025	1.001MetGln: 1.001 ± 0.023	1.017MetArg: 1.017 ± 0.026	1.232MetSer: 1.232 ± 0.027	1.154MetThr: 1.154 ± 0.026	1.238MetVal: 1.238 ± 0.027	0.168MetTrp: 0.168 ± 0.009	0.627MetTyr: 0.627 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.63AsnAla: 3.63 ± 0.057	0.292AsnCys: 0.292 ± 0.014	2.107AsnAsp: 2.107 ± 0.034	2.459AsnGlu: 2.459 ± 0.041	2.165AsnPhe: 2.165 ± 0.036	3.397AsnGly: 3.397 ± 0.06	0.907AsnHis: 0.907 ± 0.025	2.259AsnIle: 2.259 ± 0.04	1.964AsnLys: 1.964 ± 0.041	4.517AsnLeu: 4.517 ± 0.066	0.777AsnMet: 0.777 ± 0.023	2.047AsnAsn: 2.047 ± 0.051	2.864AsnPro: 2.864 ± 0.042	2.372AsnGln: 2.372 ± 0.04	2.756AsnArg: 2.756 ± 0.036	2.451AsnSer: 2.451 ± 0.039	2.694AsnThr: 2.694 ± 0.042	3.019AsnVal: 3.019 ± 0.051	0.727AsnTrp: 0.727 ± 0.021	1.995AsnTyr: 1.995 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	3.796ProAla: 3.796 ± 0.051	0.243ProCys: 0.243 ± 0.012	2.624ProAsp: 2.624 ± 0.04	3.505ProGlu: 3.505 ± 0.052	2.076ProPhe: 2.076 ± 0.036	3.103ProGly: 3.103 ± 0.048	0.825ProHis: 0.825 ± 0.023	2.449ProIle: 2.449 ± 0.037	2.165ProLys: 2.165 ± 0.042	4.293ProLeu: 4.293 ± 0.05	0.915ProMet: 0.915 ± 0.022	2.053ProAsn: 2.053 ± 0.041	1.29ProPro: 1.29 ± 0.033	1.892ProGln: 1.892 ± 0.037	1.71ProArg: 1.71 ± 0.037	2.7ProSer: 2.7 ± 0.043	2.708ProThr: 2.708 ± 0.045	3.444ProVal: 3.444 ± 0.047	0.562ProTrp: 0.562 ± 0.02	1.813ProTyr: 1.813 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	4.143GlnAla: 4.143 ± 0.056	0.225GlnCys: 0.225 ± 0.013	1.847GlnAsp: 1.847 ± 0.033	2.778GlnGlu: 2.778 ± 0.042	1.847GlnPhe: 1.847 ± 0.03	2.898GlnGly: 2.898 ± 0.044	0.969GlnHis: 0.969 ± 0.026	2.629GlnIle: 2.629 ± 0.037	2.734GlnLys: 2.734 ± 0.043	5.015GlnLeu: 5.015 ± 0.061	1.002GlnMet: 1.002 ± 0.026	2.043GlnAsn: 2.043 ± 0.033	2.287GlnPro: 2.287 ± 0.044	2.894GlnGln: 2.894 ± 0.054	2.534GlnArg: 2.534 ± 0.042	2.815GlnSer: 2.815 ± 0.04	3.07GlnThr: 3.07 ± 0.037	3.207GlnVal: 3.207 ± 0.044	0.65GlnTrp: 0.65 ± 0.021	1.84GlnTyr: 1.84 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	3.187ArgAla: 3.187 ± 0.045	0.297ArgCys: 0.297 ± 0.013	2.282ArgAsp: 2.282 ± 0.037	3.074ArgGlu: 3.074 ± 0.046	2.628ArgPhe: 2.628 ± 0.039	2.788ArgGly: 2.788 ± 0.044	0.941ArgHis: 0.941 ± 0.025	3.562ArgIle: 3.562 ± 0.049	3.25ArgLys: 3.25 ± 0.051	5.502ArgLeu: 5.502 ± 0.059	1.402ArgMet: 1.402 ± 0.028	2.575ArgAsn: 2.575 ± 0.037	2.108ArgPro: 2.108 ± 0.038	2.75ArgGln: 2.75 ± 0.046	2.687ArgArg: 2.687 ± 0.046	3.169ArgSer: 3.169 ± 0.045	3.22ArgThr: 3.22 ± 0.04	3.395ArgVal: 3.395 ± 0.048	0.884ArgTrp: 0.884 ± 0.023	2.313ArgTyr: 2.313 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	4.158SerAla: 4.158 ± 0.054	0.516SerCys: 0.516 ± 0.017	3.043SerAsp: 3.043 ± 0.045	3.358SerGlu: 3.358 ± 0.05	3.303SerPhe: 3.303 ± 0.049	4.761SerGly: 4.761 ± 0.07	1.139SerHis: 1.139 ± 0.026	3.835SerIle: 3.835 ± 0.056	3.151SerLys: 3.151 ± 0.043	6.938SerLeu: 6.938 ± 0.073	1.285SerMet: 1.285 ± 0.026	2.703SerAsn: 2.703 ± 0.053	2.76SerPro: 2.76 ± 0.047	2.746SerGln: 2.746 ± 0.039	3.133SerArg: 3.133 ± 0.045	4.12SerSer: 4.12 ± 0.06	3.879SerThr: 3.879 ± 0.05	4.429SerVal: 4.429 ± 0.062	0.938SerTrp: 0.938 ± 0.025	2.75SerTyr: 2.75 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	4.932ThrAla: 4.932 ± 0.058	0.337ThrCys: 0.337 ± 0.016	3.306ThrAsp: 3.306 ± 0.046	3.426ThrGlu: 3.426 ± 0.045	2.994ThrPhe: 2.994 ± 0.045	4.774ThrGly: 4.774 ± 0.074	1.204ThrHis: 1.204 ± 0.029	3.643ThrIle: 3.643 ± 0.052	2.839ThrLys: 2.839 ± 0.045	6.843ThrLeu: 6.843 ± 0.065	0.974ThrMet: 0.974 ± 0.022	2.727ThrAsn: 2.727 ± 0.054	3.329ThrPro: 3.329 ± 0.048	2.785ThrGln: 2.785 ± 0.042	2.732ThrArg: 2.732 ± 0.039	3.792ThrSer: 3.792 ± 0.051	3.74ThrThr: 3.74 ± 0.065	4.304ThrVal: 4.304 ± 0.066	0.742ThrTrp: 0.742 ± 0.025	2.687ThrTyr: 2.687 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	5.232ValAla: 5.232 ± 0.063	0.535ValCys: 0.535 ± 0.019	3.228ValAsp: 3.228 ± 0.043	3.893ValGlu: 3.893 ± 0.047	3.148ValPhe: 3.148 ± 0.042	4.461ValGly: 4.461 ± 0.055	1.254ValHis: 1.254 ± 0.029	3.921ValIle: 3.921 ± 0.054	3.591ValLys: 3.591 ± 0.05	6.815ValLeu: 6.815 ± 0.07	1.444ValMet: 1.444 ± 0.03	3.055ValAsn: 3.055 ± 0.047	2.972ValPro: 2.972 ± 0.038	2.855ValGln: 2.855 ± 0.044	3.56ValArg: 3.56 ± 0.049	4.635ValSer: 4.635 ± 0.062	4.144ValThr: 4.144 ± 0.058	4.668ValVal: 4.668 ± 0.068	0.911ValTrp: 0.911 ± 0.024	2.536ValTyr: 2.536 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.025TrpAla: 1.025 ± 0.027	0.108TrpCys: 0.108 ± 0.008	0.666TrpAsp: 0.666 ± 0.022	0.827TrpGlu: 0.827 ± 0.023	0.644TrpPhe: 0.644 ± 0.021	1.006TrpGly: 1.006 ± 0.025	0.308TrpHis: 0.308 ± 0.015	0.827TrpIle: 0.827 ± 0.026	0.895TrpLys: 0.895 ± 0.027	1.547TrpLeu: 1.547 ± 0.035	0.438TrpMet: 0.438 ± 0.016	0.726TrpAsn: 0.726 ± 0.023	0.455TrpPro: 0.455 ± 0.018	0.792TrpGln: 0.792 ± 0.021	0.662TrpArg: 0.662 ± 0.021	0.795TrpSer: 0.795 ± 0.022	0.788TrpThr: 0.788 ± 0.023	0.971TrpVal: 0.971 ± 0.028	0.238TrpTrp: 0.238 ± 0.012	0.547TrpTyr: 0.547 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.099TyrAla: 3.099 ± 0.042	0.321TyrCys: 0.321 ± 0.015	2.134TyrAsp: 2.134 ± 0.037	2.362TyrGlu: 2.362 ± 0.038	2.014TyrPhe: 2.014 ± 0.037	2.889TyrGly: 2.889 ± 0.043	0.84TyrHis: 0.84 ± 0.021	1.764TyrIle: 1.764 ± 0.038	1.76TyrLys: 1.76 ± 0.029	4.208TyrLeu: 4.208 ± 0.046	0.653TyrMet: 0.653 ± 0.021	1.808TyrAsn: 1.808 ± 0.044	1.799TyrPro: 1.799 ± 0.035	2.227TyrGln: 2.227 ± 0.039	2.438TyrArg: 2.438 ± 0.047	2.423TyrSer: 2.423 ± 0.042	2.603TyrThr: 2.603 ± 0.045	2.441TyrVal: 2.441 ± 0.041	0.635TyrTrp: 0.635 ± 0.02	1.711TyrTyr: 1.711 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5095 proteins (1708927 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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