Escherichia phage ECP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hendrixvirinae; Wongtaivirus; Escherichia virus ECP1

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A220NRP1|A0A220NRP1_9CAUD Head-tail joining protein OS=Escherichia phage ECP1 OX=2015807 GN=ECP1_031 PE=4 SV=1
MM1 pKa = 7.58TEE3 pKa = 4.21DD4 pKa = 4.18DD5 pKa = 5.55LYY7 pKa = 10.94PLLEE11 pKa = 4.45PLAGGQVYY19 pKa = 9.06PYY21 pKa = 9.85VAPLGSDD28 pKa = 4.08GKK30 pKa = 10.04PSVSPPWVIFSIITDD45 pKa = 3.63VAADD49 pKa = 3.76VLCGQAEE56 pKa = 4.49SAVSVQVDD64 pKa = 4.3VYY66 pKa = 11.4SSTITEE72 pKa = 3.73ARR74 pKa = 11.84TIRR77 pKa = 11.84NMALDD82 pKa = 4.42ALQVLKK88 pKa = 10.71PEE90 pKa = 5.14SIVKK94 pKa = 8.74TPGYY98 pKa = 10.36EE99 pKa = 3.52PDD101 pKa = 3.56LRR103 pKa = 11.84YY104 pKa = 9.9HH105 pKa = 6.93RR106 pKa = 11.84ATLEE110 pKa = 4.02FQVTVV115 pKa = 3.48

Molecular weight: 12.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A220NRS3|A0A220NRS3_9CAUD Uncharacterized protein OS=Escherichia phage ECP1 OX=2015807 GN=ECP1_055 PE=4 SV=1
MM1 pKa = 7.07TVVITYY7 pKa = 9.95LADD10 pKa = 4.18DD11 pKa = 3.61NARR14 pKa = 11.84NRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84ARR20 pKa = 11.84RR21 pKa = 11.84QAQRR25 pKa = 11.84EE26 pKa = 3.88QAMQEE31 pKa = 3.48QRR33 pKa = 11.84LARR36 pKa = 11.84KK37 pKa = 8.86IALKK41 pKa = 10.84LSGCVRR47 pKa = 11.84ADD49 pKa = 3.14KK50 pKa = 10.67AASLGILRR58 pKa = 11.84CKK60 pKa = 10.37KK61 pKa = 10.37ADD63 pKa = 3.52EE64 pKa = 4.66CSGSICLPNVALYY77 pKa = 10.32AAGYY81 pKa = 9.13RR82 pKa = 11.84KK83 pKa = 9.93SKK85 pKa = 10.61QLTARR90 pKa = 3.76

Molecular weight: 10.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

11741

26

1151

186.4

20.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.744 ± 0.595

1.201 ± 0.195

5.63 ± 0.312

6.149 ± 0.323

3.117 ± 0.144

7.657 ± 0.37

1.695 ± 0.262

5.553 ± 0.247

5.579 ± 0.332

7.802 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.845 ± 0.19

4.548 ± 0.209

3.62 ± 0.261

4.727 ± 0.288

6.226 ± 0.366

6.848 ± 0.356

5.936 ± 0.407

6.354 ± 0.28

1.84 ± 0.169

2.93 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski