Vibrio phage JSF35

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Chatterjeevirus; unclassified Chatterjeevirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D0Z7H1|A0A2D0Z7H1_9CAUD Uncharacterized protein OS=Vibrio phage JSF35 OX=1983612 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.33KK3 pKa = 10.75GLITVLLAAVLSACTVNIVKK23 pKa = 10.52DD24 pKa = 3.72STDD27 pKa = 3.13VRR29 pKa = 11.84ISSSYY34 pKa = 10.06EE35 pKa = 3.51GSAKK39 pKa = 10.52LSIADD44 pKa = 3.71SNFGGEE50 pKa = 4.31ADD52 pKa = 3.61STADD56 pKa = 3.49EE57 pKa = 4.64VVDD60 pKa = 4.08EE61 pKa = 5.23VIPEE65 pKa = 3.89

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D0Z224|A0A2D0Z224_9CAUD SsDNA-binding protein OS=Vibrio phage JSF35 OX=1983612 PE=4 SV=1
MM1 pKa = 7.79TITPDD6 pKa = 3.97LLRR9 pKa = 11.84KK10 pKa = 9.45LAAKK14 pKa = 10.21LDD16 pKa = 3.76AAQAEE21 pKa = 4.68VANEE25 pKa = 4.15LKK27 pKa = 10.66AVLGMCEE34 pKa = 3.99QPVQRR39 pKa = 11.84TTFQLVGKK47 pKa = 8.41PVSKK51 pKa = 10.43AVQAKK56 pKa = 9.23RR57 pKa = 11.84VEE59 pKa = 4.22RR60 pKa = 11.84EE61 pKa = 3.51MKK63 pKa = 10.1RR64 pKa = 11.84WVEE67 pKa = 3.79ANMTDD72 pKa = 4.25EE73 pKa = 4.05EE74 pKa = 4.4RR75 pKa = 11.84TRR77 pKa = 11.84QARR80 pKa = 11.84RR81 pKa = 11.84KK82 pKa = 9.11KK83 pKa = 7.81ATKK86 pKa = 10.19GVTHH90 pKa = 7.86RR91 pKa = 11.84ITKK94 pKa = 9.28EE95 pKa = 3.66INHH98 pKa = 6.49PRR100 pKa = 11.84YY101 pKa = 9.19EE102 pKa = 4.29HH103 pKa = 6.13LRR105 pKa = 11.84KK106 pKa = 10.05AGFCAGMKK114 pKa = 9.84FSIVSRR120 pKa = 11.84HH121 pKa = 4.22SLNRR125 pKa = 11.84VVVEE129 pKa = 4.93HH130 pKa = 6.39IRR132 pKa = 11.84SGKK135 pKa = 8.49RR136 pKa = 11.84AIMSTTLLEE145 pKa = 4.59TIPDD149 pKa = 3.82GQFF152 pKa = 2.73

Molecular weight: 17.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

11509

43

1127

239.8

26.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.698 ± 0.502

0.947 ± 0.194

6.03 ± 0.259

7.525 ± 0.421

4.066 ± 0.257

6.882 ± 0.357

1.781 ± 0.206

5.431 ± 0.192

7.09 ± 0.448

8.228 ± 0.337

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.902 ± 0.232

4.605 ± 0.292

3.545 ± 0.178

4.24 ± 0.313

4.97 ± 0.182

5.839 ± 0.329

5.63 ± 0.352

6.69 ± 0.282

1.277 ± 0.187

3.623 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski