Circoviridae TaCV1
Average proteome isoelectric point is 7.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L2P5L4|A0A2L2P5L4_9CIRC Putative viral capsid OS=Circoviridae TaCV1 OX=2094724 PE=4 SV=1
MM1 pKa = 7.64 SEE3 pKa = 4.08 KK4 pKa = 10.66 ASNNSKK10 pKa = 9.93 SRR12 pKa = 11.84 NYY14 pKa = 10.15 VFTIFNVGEE23 pKa = 4.33 DD24 pKa = 3.67 EE25 pKa = 4.63 SDD27 pKa = 3.44 VLGRR31 pKa = 11.84 LYY33 pKa = 11.01 EE34 pKa = 4.2 EE35 pKa = 5.16 GFCNNIFFTHH45 pKa = 6.25 EE46 pKa = 3.8 LGEE49 pKa = 4.37 NKK51 pKa = 7.64 EE52 pKa = 4.47 TPHH55 pKa = 6.18 IQGFLSCSSSRR66 pKa = 11.84 KK67 pKa = 9.8 LLTVKK72 pKa = 10.82 NKK74 pKa = 10.02 INSLFEE80 pKa = 4.01 NAPNPHH86 pKa = 5.43 VEE88 pKa = 4.08 KK89 pKa = 10.92 ANGSAYY95 pKa = 10.37 QNYY98 pKa = 9.91 IYY100 pKa = 9.19 ITKK103 pKa = 9.5 EE104 pKa = 3.72 LKK106 pKa = 10.2 EE107 pKa = 4.13 NPLLKK112 pKa = 10.35 HH113 pKa = 6.62 RR114 pKa = 11.84 IYY116 pKa = 11.09 GNRR119 pKa = 11.84 PLPPGKK125 pKa = 10.27 KK126 pKa = 9.15 RR127 pKa = 11.84 DD128 pKa = 3.63 DD129 pKa = 3.99 SKK131 pKa = 11.58 FEE133 pKa = 4.44 SYY135 pKa = 11.6 VNLLEE140 pKa = 4.74 KK141 pKa = 11.27 GEE143 pKa = 3.87 IRR145 pKa = 11.84 LRR147 pKa = 11.84 DD148 pKa = 3.55 IEE150 pKa = 4.73 RR151 pKa = 11.84 EE152 pKa = 4.03 DD153 pKa = 3.29 LAHH156 pKa = 5.44 YY157 pKa = 9.34 QRR159 pKa = 11.84 HH160 pKa = 4.51 EE161 pKa = 4.0 SFYY164 pKa = 10.85 LGVYY168 pKa = 9.26 SKK170 pKa = 11.34 LMRR173 pKa = 11.84 RR174 pKa = 11.84 GIKK177 pKa = 10.07 PPMFVSWFSGPSGSGKK193 pKa = 10.44 SFTAEE198 pKa = 4.36 AIAEE202 pKa = 4.02 ALNFDD207 pKa = 4.18 IYY209 pKa = 11.24 EE210 pKa = 4.32 VTCDD214 pKa = 3.17 NGFFGNYY221 pKa = 9.25 GGEE224 pKa = 4.9 EE225 pKa = 3.65 LALWNDD231 pKa = 3.25 YY232 pKa = 10.76 RR233 pKa = 11.84 AGCVSFHH240 pKa = 5.97 TLLRR244 pKa = 11.84 ITDD247 pKa = 3.86 RR248 pKa = 11.84 KK249 pKa = 10.84 GSMINVKK256 pKa = 9.7 GSKK259 pKa = 10.17 VFFCPRR265 pKa = 11.84 IQIFTSPDD273 pKa = 3.34 GIEE276 pKa = 4.11 SVKK279 pKa = 9.78 TYY281 pKa = 10.18 EE282 pKa = 3.84 MEE284 pKa = 4.05 YY285 pKa = 9.7 MNSHH289 pKa = 5.85 TRR291 pKa = 11.84 RR292 pKa = 11.84 LDD294 pKa = 3.33 YY295 pKa = 10.75 KK296 pKa = 10.29 FQQLLRR302 pKa = 11.84 RR303 pKa = 11.84 VSYY306 pKa = 11.14 VVDD309 pKa = 4.88 FKK311 pKa = 11.55 LSNDD315 pKa = 3.59 DD316 pKa = 3.88 SVPTLKK322 pKa = 10.23 EE323 pKa = 3.67 VKK325 pKa = 9.81 RR326 pKa = 11.84 NSEE329 pKa = 4.09 EE330 pKa = 3.95 VVKK333 pKa = 10.83 RR334 pKa = 11.84 FLGVYY339 pKa = 9.93 KK340 pKa = 10.7 YY341 pKa = 10.48 FLIDD345 pKa = 3.51 NGFEE349 pKa = 4.21 EE350 pKa = 4.91 YY351 pKa = 11.01 VKK353 pKa = 10.7 LLPALTDD360 pKa = 3.41 VEE362 pKa = 4.56 PIKK365 pKa = 11.02 INTKK369 pKa = 9.17 LVKK372 pKa = 10.17 EE373 pKa = 4.16 DD374 pKa = 4.47 EE375 pKa = 4.33 EE376 pKa = 5.11 CKK378 pKa = 10.9 VYY380 pKa = 10.13 TVDD383 pKa = 3.64 NAVSGYY389 pKa = 8.8 EE390 pKa = 3.84 HH391 pKa = 7.38 ALL393 pKa = 3.52
Molecular weight: 45.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.287
IPC2_protein 6.351
IPC_protein 6.465
Toseland 6.62
ProMoST 6.854
Dawson 6.839
Bjellqvist 6.781
Wikipedia 6.839
Rodwell 6.839
Grimsley 6.737
Solomon 6.854
Lehninger 6.854
Nozaki 7.073
DTASelect 7.22
Thurlkill 7.249
EMBOSS 7.249
Sillero 7.22
Patrickios 4.164
IPC_peptide 6.868
IPC2_peptide 6.854
IPC2.peptide.svr19 6.84
Protein with the highest isoelectric point:
>tr|A0A2L2P5L4|A0A2L2P5L4_9CIRC Putative viral capsid OS=Circoviridae TaCV1 OX=2094724 PE=4 SV=1
MM1 pKa = 7.63 SKK3 pKa = 9.95 RR4 pKa = 11.84 HH5 pKa = 4.94 TRR7 pKa = 11.84 SYY9 pKa = 10.19 LQEE12 pKa = 3.71 INTFKK17 pKa = 11.0 KK18 pKa = 9.86 KK19 pKa = 10.35 VKK21 pKa = 10.53 AIDD24 pKa = 3.47 RR25 pKa = 11.84 NEE27 pKa = 3.85 IPSYY31 pKa = 9.3 TDD33 pKa = 2.52 IMHH36 pKa = 6.35 MRR38 pKa = 11.84 NIQLALEE45 pKa = 4.1 FLTINANPEE54 pKa = 3.91 KK55 pKa = 10.85 AIVTQKK61 pKa = 11.23 AFAKK65 pKa = 9.27 SKK67 pKa = 11.04 NMGTATLRR75 pKa = 11.84 KK76 pKa = 8.75 AIKK79 pKa = 9.82 EE80 pKa = 3.97 ITHH83 pKa = 5.39 SGMRR87 pKa = 11.84 KK88 pKa = 9.48 VNPKK92 pKa = 10.18 SNNIQKK98 pKa = 9.47 YY99 pKa = 9.48 QNDD102 pKa = 3.97 KK103 pKa = 11.45 KK104 pKa = 11.1 EE105 pKa = 4.13 LLQKK109 pKa = 8.61 TRR111 pKa = 11.84 EE112 pKa = 4.07 YY113 pKa = 11.24 GEE115 pKa = 3.87 QALTQEE121 pKa = 4.36 EE122 pKa = 4.44 QLRR125 pKa = 11.84 LQQIQEE131 pKa = 4.22 KK132 pKa = 9.07 EE133 pKa = 3.85 LQRR136 pKa = 11.84 IEE138 pKa = 4.15 KK139 pKa = 10.04 CKK141 pKa = 9.37 KK142 pKa = 8.18 TKK144 pKa = 9.73 TKK146 pKa = 10.92 AKK148 pKa = 10.34 AKK150 pKa = 9.1 HH151 pKa = 5.77 TDD153 pKa = 2.74 NEE155 pKa = 4.5 YY156 pKa = 10.63 EE157 pKa = 3.89 VRR159 pKa = 11.84 GGYY162 pKa = 9.13 RR163 pKa = 11.84 EE164 pKa = 4.3 HH165 pKa = 6.95 EE166 pKa = 4.07 NDD168 pKa = 3.81 YY169 pKa = 11.41 EE170 pKa = 4.13 EE171 pKa = 5.29 QEE173 pKa = 4.36 EE174 pKa = 4.25 NTIKK178 pKa = 10.52 HH179 pKa = 5.81 RR180 pKa = 11.84 NINRR184 pKa = 11.84 ALQTLINSKK193 pKa = 9.19 NEE195 pKa = 3.81 KK196 pKa = 9.23 EE197 pKa = 4.06 QLRR200 pKa = 11.84 QKK202 pKa = 9.93 LTIDD206 pKa = 3.48 EE207 pKa = 4.37
Molecular weight: 24.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.891
IPC2_protein 8.99
IPC_protein 8.873
Toseland 9.897
ProMoST 9.458
Dawson 10.043
Bjellqvist 9.648
Wikipedia 10.16
Rodwell 10.657
Grimsley 10.087
Solomon 10.072
Lehninger 10.043
Nozaki 9.867
DTASelect 9.648
Thurlkill 9.911
EMBOSS 10.277
Sillero 9.955
Patrickios 10.306
IPC_peptide 10.072
IPC2_peptide 8.024
IPC2.peptide.svr19 7.929
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
600
207
393
300.0
34.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.333 ± 0.814
1.167 ± 0.38
4.167 ± 0.705
10.0 ± 0.886
4.5 ± 1.696
5.0 ± 1.437
2.5 ± 0.222
6.0 ± 0.962
10.167 ± 2.137
8.167 ± 0.512
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.833 ± 0.324
7.333 ± 0.489
3.0 ± 0.862
3.833 ± 2.435
6.0 ± 0.424
6.333 ± 1.641
5.333 ± 1.332
5.167 ± 1.798
0.333 ± 0.185
4.833 ± 0.807
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here