Odonata-associated circular virus-17
Average proteome isoelectric point is 7.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UI13|A0A0B4UI13_9VIRU Replication-associated protein OS=Odonata-associated circular virus-17 OX=1592117 PE=4 SV=1
MM1 pKa = 7.11 TSEE4 pKa = 4.34 VMTSIAPGNTKK15 pKa = 9.8 PVALKK20 pKa = 10.36 ARR22 pKa = 11.84 AYY24 pKa = 10.51 QFTLNQPDD32 pKa = 4.27 VYY34 pKa = 11.33 DD35 pKa = 3.54 KK36 pKa = 11.34 LKK38 pKa = 11.21 NILTGLKK45 pKa = 9.66 TMDD48 pKa = 3.55 YY49 pKa = 10.4 LISCRR54 pKa = 11.84 EE55 pKa = 3.93 TAPSTGHH62 pKa = 4.66 VHH64 pKa = 4.23 MHH66 pKa = 5.87 VYY68 pKa = 10.84 AHH70 pKa = 7.03 FSQQYY75 pKa = 9.48 RR76 pKa = 11.84 LPKK79 pKa = 9.93 KK80 pKa = 10.29 IRR82 pKa = 11.84 DD83 pKa = 3.86 LGCHH87 pKa = 6.22 IEE89 pKa = 4.0 ICLVGAKK96 pKa = 9.75 QNIAYY101 pKa = 8.87 IRR103 pKa = 11.84 KK104 pKa = 9.17 GGDD107 pKa = 3.1 IIDD110 pKa = 4.77 EE111 pKa = 4.6 IGDD114 pKa = 3.83 EE115 pKa = 4.07 PHH117 pKa = 6.09 QGARR121 pKa = 11.84 TVGEE125 pKa = 4.31 LMEE128 pKa = 6.42 ADD130 pKa = 3.53 IKK132 pKa = 11.43 SIDD135 pKa = 3.32 PHH137 pKa = 7.82 LYY139 pKa = 10.06 RR140 pKa = 11.84 IKK142 pKa = 10.63 KK143 pKa = 10.07 QIDD146 pKa = 3.59 AEE148 pKa = 4.21 QHH150 pKa = 6.37 DD151 pKa = 5.7 LDD153 pKa = 4.59 TFMSMLDD160 pKa = 3.77 EE161 pKa = 5.75 IEE163 pKa = 5.36 ADD165 pKa = 3.72 DD166 pKa = 4.86 LKK168 pKa = 11.22 APTVIYY174 pKa = 9.35 ITGGTGKK181 pKa = 10.52 GKK183 pKa = 9.0 TYY185 pKa = 10.53 SAYY188 pKa = 9.67 KK189 pKa = 10.01 RR190 pKa = 11.84 ALKK193 pKa = 9.92 EE194 pKa = 3.89 YY195 pKa = 8.86 PKK197 pKa = 10.86 KK198 pKa = 10.86 KK199 pKa = 9.65 IGKK202 pKa = 8.31 LTLKK206 pKa = 10.49 NDD208 pKa = 4.65 FIDD211 pKa = 4.76 IVNGDD216 pKa = 3.48 NAEE219 pKa = 4.15 CFVIEE224 pKa = 4.5 EE225 pKa = 4.39 FRR227 pKa = 11.84 PSQIKK232 pKa = 10.58 ASDD235 pKa = 3.47 FLQLTDD241 pKa = 3.67 KK242 pKa = 11.17 YY243 pKa = 11.22 GYY245 pKa = 9.65 RR246 pKa = 11.84 CNIKK250 pKa = 10.62 GGFATLRR257 pKa = 11.84 PKK259 pKa = 10.17 MIIICSIIQPEE270 pKa = 4.47 HH271 pKa = 6.28 IYY273 pKa = 10.7 KK274 pKa = 10.81 EE275 pKa = 4.23 EE276 pKa = 4.02 VNKK279 pKa = 10.37 QFLRR283 pKa = 11.84 RR284 pKa = 11.84 ITEE287 pKa = 4.47 RR288 pKa = 11.84 IDD290 pKa = 3.48 LGPEE294 pKa = 4.16 LDD296 pKa = 4.75 PVDD299 pKa = 4.38 EE300 pKa = 5.0 LYY302 pKa = 10.78 EE303 pKa = 4.02
Molecular weight: 34.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.108
IPC2_protein 6.109
IPC_protein 6.211
Toseland 6.491
ProMoST 6.427
Dawson 6.547
Bjellqvist 6.517
Wikipedia 6.576
Rodwell 6.547
Grimsley 6.62
Solomon 6.561
Lehninger 6.561
Nozaki 6.81
DTASelect 6.985
Thurlkill 7.015
EMBOSS 7.0
Sillero 6.942
Patrickios 4.177
IPC_peptide 6.576
IPC2_peptide 6.737
IPC2.peptide.svr19 6.797
Protein with the highest isoelectric point:
>tr|A0A0B4UI13|A0A0B4UI13_9VIRU Replication-associated protein OS=Odonata-associated circular virus-17 OX=1592117 PE=4 SV=1
MM1 pKa = 7.48 VIFLEE6 pKa = 4.67 PSFSLVKK13 pKa = 10.36 MSQTQIVDD21 pKa = 3.4 YY22 pKa = 10.94 VFYY25 pKa = 10.23 SCYY28 pKa = 8.42 MAYY31 pKa = 10.26 GRR33 pKa = 11.84 RR34 pKa = 11.84 STYY37 pKa = 9.23 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 10.06 GRR42 pKa = 11.84 RR43 pKa = 11.84 GNRR46 pKa = 11.84 SLTTRR51 pKa = 11.84 RR52 pKa = 11.84 IFNNKK57 pKa = 7.4 GAKK60 pKa = 9.28 AQAKK64 pKa = 9.37 QIYY67 pKa = 8.6 ALKK70 pKa = 10.14 RR71 pKa = 11.84 AVNRR75 pKa = 11.84 VRR77 pKa = 11.84 AQCKK81 pKa = 10.13 PEE83 pKa = 3.77 VKK85 pKa = 9.92 YY86 pKa = 11.23 VKK88 pKa = 10.54 SEE90 pKa = 4.0 TQNRR94 pKa = 11.84 ALGLEE99 pKa = 4.1 HH100 pKa = 6.68 QGASFLQYY108 pKa = 10.21 YY109 pKa = 9.22 AQLPMPGISAGTGDD123 pKa = 4.22 GNRR126 pKa = 11.84 IGDD129 pKa = 4.03 NVRR132 pKa = 11.84 LLPMKK137 pKa = 10.5 LGINIRR143 pKa = 11.84 YY144 pKa = 8.25 EE145 pKa = 4.0 EE146 pKa = 4.27 YY147 pKa = 10.23 MNSLTKK153 pKa = 10.09 TYY155 pKa = 10.39 PVDD158 pKa = 3.22 IPLNSTGGQVRR169 pKa = 11.84 IIAVQAITAHH179 pKa = 5.97 SNEE182 pKa = 4.15 PQLVDD187 pKa = 3.94 LMNANEE193 pKa = 4.09 YY194 pKa = 11.43 NMGAIGAAGMMNMSFKK210 pKa = 10.73 RR211 pKa = 11.84 GITSHH216 pKa = 6.4 YY217 pKa = 9.17 KK218 pKa = 9.22 ILMNKK223 pKa = 8.42 VVTVSKK229 pKa = 10.61 DD230 pKa = 3.21 RR231 pKa = 11.84 PIVNKK236 pKa = 10.23 RR237 pKa = 11.84 YY238 pKa = 9.95 LIRR241 pKa = 11.84 PAIKK245 pKa = 9.16 HH246 pKa = 5.6 LKK248 pKa = 8.42 VGRR251 pKa = 11.84 RR252 pKa = 11.84 TDD254 pKa = 3.27 STT256 pKa = 3.82
Molecular weight: 28.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.926
IPC_protein 10.496
Toseland 10.467
ProMoST 10.175
Dawson 10.643
Bjellqvist 10.335
Wikipedia 10.833
Rodwell 10.994
Grimsley 10.716
Solomon 10.687
Lehninger 10.643
Nozaki 10.438
DTASelect 10.335
Thurlkill 10.496
EMBOSS 10.862
Sillero 10.555
Patrickios 10.643
IPC_peptide 10.687
IPC2_peptide 9.194
IPC2.peptide.svr19 8.486
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
559
256
303
279.5
31.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.619 ± 0.533
1.431 ± 0.431
5.367 ± 1.748
5.188 ± 1.37
2.683 ± 0.225
6.977 ± 0.296
2.326 ± 0.507
8.945 ± 1.27
8.229 ± 0.795
7.513 ± 0.32
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.578 ± 0.737
4.651 ± 1.321
4.293 ± 0.257
4.293 ± 0.262
6.619 ± 1.571
4.83 ± 0.943
5.725 ± 0.17
5.367 ± 1.105
0.0 ± 0.0
5.367 ± 0.327
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here