bacterium

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5998 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q5NKM6|A0A4Q5NKM6_9BACT Uncharacterized protein OS=bacterium OX=1869227 GN=EON83_27580 PE=4 SV=1
MM1 pKa = 7.14SHH3 pKa = 4.82QTRR6 pKa = 11.84EE7 pKa = 4.02EE8 pKa = 4.4LEE10 pKa = 4.51LGTNTIEE17 pKa = 4.14IEE19 pKa = 4.15AVPTMKK25 pKa = 10.36EE26 pKa = 3.81VLYY29 pKa = 10.39LAEE32 pKa = 6.0DD33 pKa = 3.83GTEE36 pKa = 3.84VDD38 pKa = 4.08DD39 pKa = 4.98PPRR42 pKa = 11.84EE43 pKa = 4.03LVGIAIDD50 pKa = 3.34MGGNQVYY57 pKa = 10.39LSLGGRR63 pKa = 11.84QPGAIYY69 pKa = 10.14LWLNWEE75 pKa = 4.9DD76 pKa = 3.57IYY78 pKa = 11.38FVADD82 pKa = 3.41SFDD85 pKa = 3.82EE86 pKa = 4.85FVHH89 pKa = 6.26SLYY92 pKa = 11.23VVDD95 pKa = 5.09

Molecular weight: 10.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q5NKE4|A0A4Q5NKE4_9BACT Magnesium transport protein CorA OS=bacterium OX=1869227 GN=corA PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.68QPKK8 pKa = 9.4KK9 pKa = 9.39RR10 pKa = 11.84PRR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 8.61LGFRR19 pKa = 11.84ARR21 pKa = 11.84MSSPGGRR28 pKa = 11.84KK29 pKa = 9.07VISRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84YY40 pKa = 9.39KK41 pKa = 10.08LTQVV45 pKa = 3.23

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5998

0

5998

2076602

23

2376

346.2

37.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.355 ± 0.034

0.936 ± 0.009

5.234 ± 0.024

5.754 ± 0.038

4.019 ± 0.023

8.032 ± 0.034

1.927 ± 0.017

4.911 ± 0.022

4.132 ± 0.026

9.792 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.065 ± 0.014

3.846 ± 0.026

5.386 ± 0.026

3.855 ± 0.018

6.13 ± 0.031

6.484 ± 0.028

5.979 ± 0.039

6.858 ± 0.029

1.736 ± 0.018

2.567 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski