Amino acid dipepetide frequency for Macrococcus lamae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.165AlaAla: 6.165 ± 0.124
0.55AlaCys: 0.55 ± 0.035
3.924AlaAsp: 3.924 ± 0.082
4.776AlaGlu: 4.776 ± 0.101
2.966AlaPhe: 2.966 ± 0.082
5.169AlaGly: 5.169 ± 0.107
1.451AlaHis: 1.451 ± 0.05
5.652AlaIle: 5.652 ± 0.117
4.155AlaLys: 4.155 ± 0.088
7.123AlaLeu: 7.123 ± 0.126
2.103AlaMet: 2.103 ± 0.067
2.257AlaAsn: 2.257 ± 0.06
2.018AlaPro: 2.018 ± 0.066
2.133AlaGln: 2.133 ± 0.065
2.793AlaArg: 2.793 ± 0.078
3.656AlaSer: 3.656 ± 0.089
3.289AlaThr: 3.289 ± 0.085
5.855AlaVal: 5.855 ± 0.095
0.459AlaTrp: 0.459 ± 0.03
2.242AlaTyr: 2.242 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
0.391CysAla: 0.391 ± 0.026
0.074CysCys: 0.074 ± 0.012
0.419CysAsp: 0.419 ± 0.028
0.351CysGlu: 0.351 ± 0.027
0.274CysPhe: 0.274 ± 0.024
0.596CysGly: 0.596 ± 0.031
0.229CysHis: 0.229 ± 0.022
0.449CysIle: 0.449 ± 0.028
0.296CysLys: 0.296 ± 0.024
0.642CysLeu: 0.642 ± 0.035
0.167CysMet: 0.167 ± 0.016
0.257CysAsn: 0.257 ± 0.023
0.324CysPro: 0.324 ± 0.026
0.246CysGln: 0.246 ± 0.021
0.244CysArg: 0.244 ± 0.019
0.375CysSer: 0.375 ± 0.025
0.395CysThr: 0.395 ± 0.026
0.37CysVal: 0.37 ± 0.027
0.046CysTrp: 0.046 ± 0.009
0.296CysTyr: 0.296 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
3.788AspAla: 3.788 ± 0.084
0.395AspCys: 0.395 ± 0.029
3.387AspAsp: 3.387 ± 0.088
4.738AspGlu: 4.738 ± 0.095
2.779AspPhe: 2.779 ± 0.067
3.435AspGly: 3.435 ± 0.082
1.413AspHis: 1.413 ± 0.056
4.964AspIle: 4.964 ± 0.089
4.082AspLys: 4.082 ± 0.092
4.599AspLeu: 4.599 ± 0.103
1.672AspMet: 1.672 ± 0.051
2.694AspAsn: 2.694 ± 0.07
1.662AspPro: 1.662 ± 0.058
2.01AspGln: 2.01 ± 0.052
2.368AspArg: 2.368 ± 0.064
2.914AspSer: 2.914 ± 0.072
2.868AspThr: 2.868 ± 0.069
4.165AspVal: 4.165 ± 0.09
0.501AspTrp: 0.501 ± 0.028
3.032AspTyr: 3.032 ± 0.064
0.0AspXaa: 0.0 ± 0.0
Glu
4.976GluAla: 4.976 ± 0.097
0.311GluCys: 0.311 ± 0.023
3.838GluAsp: 3.838 ± 0.077
5.972GluGlu: 5.972 ± 0.138
2.431GluPhe: 2.431 ± 0.074
3.782GluGly: 3.782 ± 0.1
1.572GluHis: 1.572 ± 0.047
5.158GluIle: 5.158 ± 0.102
5.058GluLys: 5.058 ± 0.096
7.149GluLeu: 7.149 ± 0.122
2.283GluMet: 2.283 ± 0.071
3.204GluAsn: 3.204 ± 0.081
1.82GluPro: 1.82 ± 0.066
3.711GluGln: 3.711 ± 0.103
3.656GluArg: 3.656 ± 0.1
3.526GluSer: 3.526 ± 0.071
4.028GluThr: 4.028 ± 0.078
5.036GluVal: 5.036 ± 0.095
0.634GluTrp: 0.634 ± 0.032
2.131GluTyr: 2.131 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
2.688PheAla: 2.688 ± 0.082
0.29PheCys: 0.29 ± 0.021
2.86PheAsp: 2.86 ± 0.068
2.85PheGlu: 2.85 ± 0.062
2.137PhePhe: 2.137 ± 0.07
3.132PheGly: 3.132 ± 0.09
0.878PheHis: 0.878 ± 0.042
4.16PheIle: 4.16 ± 0.099
3.119PheLys: 3.119 ± 0.071
4.067PheLeu: 4.067 ± 0.096
1.292PheMet: 1.292 ± 0.054
2.842PheAsn: 2.842 ± 0.064
1.382PhePro: 1.382 ± 0.045
1.302PheGln: 1.302 ± 0.043
1.472PheArg: 1.472 ± 0.052
2.971PheSer: 2.971 ± 0.075
2.468PheThr: 2.468 ± 0.064
2.996PheVal: 2.996 ± 0.076
0.393PheTrp: 0.393 ± 0.031
1.793PheTyr: 1.793 ± 0.052
0.0PheXaa: 0.0 ± 0.0
Gly
4.432GlyAla: 4.432 ± 0.102
0.485GlyCys: 0.485 ± 0.028
3.204GlyAsp: 3.204 ± 0.083
3.857GlyGlu: 3.857 ± 0.083
3.079GlyPhe: 3.079 ± 0.079
4.448GlyGly: 4.448 ± 0.128
1.605GlyHis: 1.605 ± 0.054
5.606GlyIle: 5.606 ± 0.11
4.162GlyLys: 4.162 ± 0.097
6.121GlyLeu: 6.121 ± 0.124
2.051GlyMet: 2.051 ± 0.068
2.47GlyAsn: 2.47 ± 0.069
1.625GlyPro: 1.625 ± 0.044
2.323GlyGln: 2.323 ± 0.063
2.847GlyArg: 2.847 ± 0.074
3.597GlySer: 3.597 ± 0.076
3.955GlyThr: 3.955 ± 0.097
4.534GlyVal: 4.534 ± 0.088
0.577GlyTrp: 0.577 ± 0.034
2.807GlyTyr: 2.807 ± 0.071
0.0GlyXaa: 0.0 ± 0.0
His
1.446HisAla: 1.446 ± 0.054
0.167HisCys: 0.167 ± 0.017
1.415HisAsp: 1.415 ± 0.046
1.5HisGlu: 1.5 ± 0.045
1.169HisPhe: 1.169 ± 0.052
1.404HisGly: 1.404 ± 0.055
0.924HisHis: 0.924 ± 0.05
1.926HisIle: 1.926 ± 0.053
0.981HisLys: 0.981 ± 0.042
2.326HisLeu: 2.326 ± 0.068
0.716HisMet: 0.716 ± 0.034
0.891HisAsn: 0.891 ± 0.038
1.191HisPro: 1.191 ± 0.047
1.097HisGln: 1.097 ± 0.045
0.916HisArg: 0.916 ± 0.038
1.187HisSer: 1.187 ± 0.045
1.202HisThr: 1.202 ± 0.039
1.636HisVal: 1.636 ± 0.051
0.182HisTrp: 0.182 ± 0.015
1.145HisTyr: 1.145 ± 0.041
0.0HisXaa: 0.0 ± 0.0
Ile
6.008IleAla: 6.008 ± 0.105
0.645IleCys: 0.645 ± 0.034
5.305IleAsp: 5.305 ± 0.095
5.844IleGlu: 5.844 ± 0.098
3.386IlePhe: 3.386 ± 0.108
5.762IleGly: 5.762 ± 0.117
1.775IleHis: 1.775 ± 0.057
7.131IleIle: 7.131 ± 0.146
5.487IleLys: 5.487 ± 0.092
6.679IleLeu: 6.679 ± 0.14
2.144IleMet: 2.144 ± 0.054
4.147IleAsn: 4.147 ± 0.087
2.989IlePro: 2.989 ± 0.08
2.555IleGln: 2.555 ± 0.078
2.906IleArg: 2.906 ± 0.074
5.048IleSer: 5.048 ± 0.099
4.598IleThr: 4.598 ± 0.095
5.552IleVal: 5.552 ± 0.11
0.532IleTrp: 0.532 ± 0.031
2.871IleTyr: 2.871 ± 0.075
0.0IleXaa: 0.0 ± 0.0
Lys
4.504LysAla: 4.504 ± 0.094
0.305LysCys: 0.305 ± 0.024
4.277LysAsp: 4.277 ± 0.101
5.875LysGlu: 5.875 ± 0.123
2.331LysPhe: 2.331 ± 0.065
3.829LysGly: 3.829 ± 0.084
1.438LysHis: 1.438 ± 0.051
4.481LysIle: 4.481 ± 0.09
5.315LysLys: 5.315 ± 0.114
5.729LysLeu: 5.729 ± 0.103
2.173LysMet: 2.173 ± 0.055
3.426LysAsn: 3.426 ± 0.087
2.088LysPro: 2.088 ± 0.057
3.004LysGln: 3.004 ± 0.075
3.146LysArg: 3.146 ± 0.064
3.731LysSer: 3.731 ± 0.079
3.793LysThr: 3.793 ± 0.089
4.599LysVal: 4.599 ± 0.096
0.568LysTrp: 0.568 ± 0.029
2.527LysTyr: 2.527 ± 0.073
0.0LysXaa: 0.0 ± 0.0
Leu
6.516LeuAla: 6.516 ± 0.111
0.601LeuCys: 0.601 ± 0.028
4.884LeuAsp: 4.884 ± 0.087
5.932LeuGlu: 5.932 ± 0.114
4.715LeuPhe: 4.715 ± 0.121
5.872LeuGly: 5.872 ± 0.127
1.856LeuHis: 1.856 ± 0.058
7.958LeuIle: 7.958 ± 0.145
6.895LeuLys: 6.895 ± 0.103
10.052LeuLeu: 10.052 ± 0.188
3.012LeuMet: 3.012 ± 0.074
5.117LeuAsn: 5.117 ± 0.089
3.752LeuPro: 3.752 ± 0.085
2.95LeuGln: 2.95 ± 0.077
3.11LeuArg: 3.11 ± 0.086
6.632LeuSer: 6.632 ± 0.109
6.098LeuThr: 6.098 ± 0.116
5.919LeuVal: 5.919 ± 0.109
0.577LeuTrp: 0.577 ± 0.031
3.281LeuTyr: 3.281 ± 0.076
0.0LeuXaa: 0.0 ± 0.0
Met
2.093MetAla: 2.093 ± 0.062
0.152MetCys: 0.152 ± 0.016
1.582MetAsp: 1.582 ± 0.053
1.739MetGlu: 1.739 ± 0.054
1.169MetPhe: 1.169 ± 0.045
1.567MetGly: 1.567 ± 0.058
0.603MetHis: 0.603 ± 0.035
2.635MetIle: 2.635 ± 0.067
2.575MetLys: 2.575 ± 0.061
2.848MetLeu: 2.848 ± 0.072
1.212MetMet: 1.212 ± 0.043
1.913MetAsn: 1.913 ± 0.062
1.117MetPro: 1.117 ± 0.045
1.02MetGln: 1.02 ± 0.04
1.181MetArg: 1.181 ± 0.039
1.944MetSer: 1.944 ± 0.06
2.383MetThr: 2.383 ± 0.059
1.628MetVal: 1.628 ± 0.053
0.18MetTrp: 0.18 ± 0.016
0.829MetTyr: 0.829 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
3.02AsnAla: 3.02 ± 0.073
0.308AsnCys: 0.308 ± 0.023
2.947AsnAsp: 2.947 ± 0.075
3.497AsnGlu: 3.497 ± 0.071
1.933AsnPhe: 1.933 ± 0.063
3.099AsnGly: 3.099 ± 0.081
1.358AsnHis: 1.358 ± 0.051
3.831AsnIle: 3.831 ± 0.084
3.207AsnLys: 3.207 ± 0.074
3.772AsnLeu: 3.772 ± 0.08
1.299AsnMet: 1.299 ± 0.045
2.588AsnAsn: 2.588 ± 0.069
2.01AsnPro: 2.01 ± 0.057
2.247AsnGln: 2.247 ± 0.068
2.151AsnArg: 2.151 ± 0.068
2.254AsnSer: 2.254 ± 0.072
2.223AsnThr: 2.223 ± 0.058
3.123AsnVal: 3.123 ± 0.067
0.403AsnTrp: 0.403 ± 0.025
2.031AsnTyr: 2.031 ± 0.061
0.0AsnXaa: 0.0 ± 0.0
Pro
2.309ProAla: 2.309 ± 0.061
0.183ProCys: 0.183 ± 0.017
2.175ProAsp: 2.175 ± 0.058
2.861ProGlu: 2.861 ± 0.074
1.813ProPhe: 1.813 ± 0.049
2.249ProGly: 2.249 ± 0.063
0.786ProHis: 0.786 ± 0.036
2.457ProIle: 2.457 ± 0.065
2.046ProLys: 2.046 ± 0.058
3.241ProLeu: 3.241 ± 0.072
0.902ProMet: 0.902 ± 0.041
1.428ProAsn: 1.428 ± 0.047
0.744ProPro: 0.744 ± 0.038
0.924ProGln: 0.924 ± 0.039
1.02ProArg: 1.02 ± 0.043
1.99ProSer: 1.99 ± 0.053
1.777ProThr: 1.777 ± 0.06
3.082ProVal: 3.082 ± 0.078
0.28ProTrp: 0.28 ± 0.024
1.412ProTyr: 1.412 ± 0.053
0.0ProXaa: 0.0 ± 0.0
Gln
2.542GlnAla: 2.542 ± 0.07
0.172GlnCys: 0.172 ± 0.017
1.725GlnAsp: 1.725 ± 0.06
2.25GlnGlu: 2.25 ± 0.067
1.877GlnPhe: 1.877 ± 0.049
2.005GlnGly: 2.005 ± 0.06
0.989GlnHis: 0.989 ± 0.04
2.517GlnIle: 2.517 ± 0.066
2.234GlnLys: 2.234 ± 0.069
4.832GlnLeu: 4.832 ± 0.108
1.299GlnMet: 1.299 ± 0.047
1.628GlnAsn: 1.628 ± 0.055
1.279GlnPro: 1.279 ± 0.054
2.015GlnGln: 2.015 ± 0.077
1.469GlnArg: 1.469 ± 0.056
2.349GlnSer: 2.349 ± 0.063
1.954GlnThr: 1.954 ± 0.057
2.39GlnVal: 2.39 ± 0.057
0.313GlnTrp: 0.313 ± 0.024
1.561GlnTyr: 1.561 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
2.494ArgAla: 2.494 ± 0.072
0.195ArgCys: 0.195 ± 0.019
2.182ArgAsp: 2.182 ± 0.062
2.961ArgGlu: 2.961 ± 0.077
1.895ArgPhe: 1.895 ± 0.053
2.244ArgGly: 2.244 ± 0.062
1.014ArgHis: 1.014 ± 0.042
3.141ArgIle: 3.141 ± 0.084
2.789ArgLys: 2.789 ± 0.078
4.087ArgLeu: 4.087 ± 0.089
1.386ArgMet: 1.386 ± 0.054
1.903ArgAsn: 1.903 ± 0.057
1.307ArgPro: 1.307 ± 0.047
1.851ArgGln: 1.851 ± 0.064
2.133ArgArg: 2.133 ± 0.066
2.036ArgSer: 2.036 ± 0.057
2.383ArgThr: 2.383 ± 0.069
2.599ArgVal: 2.599 ± 0.072
0.316ArgTrp: 0.316 ± 0.018
1.635ArgTyr: 1.635 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
3.674SerAla: 3.674 ± 0.099
0.362SerCys: 0.362 ± 0.024
3.25SerAsp: 3.25 ± 0.063
3.908SerGlu: 3.908 ± 0.089
3.004SerPhe: 3.004 ± 0.073
4.067SerGly: 4.067 ± 0.087
1.527SerHis: 1.527 ± 0.053
4.586SerIle: 4.586 ± 0.102
3.561SerLys: 3.561 ± 0.073
5.603SerLeu: 5.603 ± 0.099
1.674SerMet: 1.674 ± 0.056
2.491SerAsn: 2.491 ± 0.062
1.885SerPro: 1.885 ± 0.054
2.054SerGln: 2.054 ± 0.059
2.491SerArg: 2.491 ± 0.069
3.549SerSer: 3.549 ± 0.084
3.245SerThr: 3.245 ± 0.082
3.978SerVal: 3.978 ± 0.084
0.485SerTrp: 0.485 ± 0.027
2.534SerTyr: 2.534 ± 0.07
0.0SerXaa: 0.0 ± 0.0
Thr
4.498ThrAla: 4.498 ± 0.105
0.359ThrCys: 0.359 ± 0.027
3.327ThrAsp: 3.327 ± 0.072
3.944ThrGlu: 3.944 ± 0.093
2.655ThrPhe: 2.655 ± 0.06
4.201ThrGly: 4.201 ± 0.096
1.299ThrHis: 1.299 ± 0.047
4.661ThrIle: 4.661 ± 0.092
3.272ThrLys: 3.272 ± 0.081
5.706ThrLeu: 5.706 ± 0.103
1.527ThrMet: 1.527 ± 0.051
2.313ThrAsn: 2.313 ± 0.058
2.29ThrPro: 2.29 ± 0.072
1.613ThrGln: 1.613 ± 0.052
1.987ThrArg: 1.987 ± 0.058
3.141ThrSer: 3.141 ± 0.081
3.386ThrThr: 3.386 ± 0.088
4.489ThrVal: 4.489 ± 0.09
0.447ThrTrp: 0.447 ± 0.033
2.113ThrTyr: 2.113 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
4.44ValAla: 4.44 ± 0.1
0.529ValCys: 0.529 ± 0.033
3.952ValAsp: 3.952 ± 0.085
4.575ValGlu: 4.575 ± 0.091
2.958ValPhe: 2.958 ± 0.078
4.069ValGly: 4.069 ± 0.095
1.374ValHis: 1.374 ± 0.048
6.381ValIle: 6.381 ± 0.1
5.058ValLys: 5.058 ± 0.096
6.501ValLeu: 6.501 ± 0.112
2.208ValMet: 2.208 ± 0.057
3.412ValAsn: 3.412 ± 0.072
2.567ValPro: 2.567 ± 0.066
2.231ValGln: 2.231 ± 0.063
2.662ValArg: 2.662 ± 0.067
4.304ValSer: 4.304 ± 0.081
4.732ValThr: 4.732 ± 0.082
4.922ValVal: 4.922 ± 0.104
0.49ValTrp: 0.49 ± 0.031
2.308ValTyr: 2.308 ± 0.072
0.0ValXaa: 0.0 ± 0.0
Trp
0.436TrpAla: 0.436 ± 0.025
0.082TrpCys: 0.082 ± 0.011
0.362TrpAsp: 0.362 ± 0.026
0.346TrpGlu: 0.346 ± 0.025
0.421TrpPhe: 0.421 ± 0.028
0.449TrpGly: 0.449 ± 0.027
0.226TrpHis: 0.226 ± 0.019
0.686TrpIle: 0.686 ± 0.039
0.477TrpLys: 0.477 ± 0.03
0.945TrpLeu: 0.945 ± 0.041
0.305TrpMet: 0.305 ± 0.022
0.416TrpAsn: 0.416 ± 0.027
0.236TrpPro: 0.236 ± 0.019
0.365TrpGln: 0.365 ± 0.029
0.288TrpArg: 0.288 ± 0.023
0.437TrpSer: 0.437 ± 0.025
0.416TrpThr: 0.416 ± 0.025
0.498TrpVal: 0.498 ± 0.03
0.095TrpTrp: 0.095 ± 0.012
0.324TrpTyr: 0.324 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.316TyrAla: 2.316 ± 0.061
0.277TyrCys: 0.277 ± 0.022
2.457TyrAsp: 2.457 ± 0.066
2.498TyrGlu: 2.498 ± 0.071
2.1TyrPhe: 2.1 ± 0.058
2.406TyrGly: 2.406 ± 0.06
1.104TyrHis: 1.104 ± 0.043
2.981TyrIle: 2.981 ± 0.075
2.373TyrLys: 2.373 ± 0.062
3.707TyrLeu: 3.707 ± 0.088
0.948TyrMet: 0.948 ± 0.033
1.902TyrAsn: 1.902 ± 0.055
1.394TyrPro: 1.394 ± 0.053
1.749TyrGln: 1.749 ± 0.059
1.712TyrArg: 1.712 ± 0.053
2.285TyrSer: 2.285 ± 0.057
2.036TyrThr: 2.036 ± 0.064
2.296TyrVal: 2.296 ± 0.06
0.332TyrTrp: 0.332 ± 0.025
1.733TyrTyr: 1.733 ± 0.06
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2076 proteins (610545 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski