Devosia sp. H239

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Devosiaceae; Devosia

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3157 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M1SXX9|A0A6M1SXX9_9RHIZ Polyphosphate kinase OS=Devosia sp. H239 OX=2714858 GN=ppk PE=3 SV=1
MM1 pKa = 7.78DD2 pKa = 3.84WSLFGLVPMLFGMAPADD19 pKa = 3.75SPRR22 pKa = 11.84ALLDD26 pKa = 4.9AIYY29 pKa = 10.61QPLQSGEE36 pKa = 4.13VVNLEE41 pKa = 3.97EE42 pKa = 5.25HH43 pKa = 6.68YY44 pKa = 10.94SDD46 pKa = 4.98HH47 pKa = 6.27LQALIANNLEE57 pKa = 4.13LNAVDD62 pKa = 3.72VTGAAIDD69 pKa = 3.97PEE71 pKa = 4.68APGIVEE77 pKa = 5.3FNPFLNGTDD86 pKa = 3.27ASLVNLSVTEE96 pKa = 4.1PVVQGQSAVALVSFDD111 pKa = 3.58TASVPTTLSISMINDD126 pKa = 4.04GAWKK130 pKa = 9.64IDD132 pKa = 3.51DD133 pKa = 3.98VASVGAGEE141 pKa = 4.0KK142 pKa = 9.05WLYY145 pKa = 10.89SWLLQYY151 pKa = 11.05DD152 pKa = 4.81PYY154 pKa = 11.11NQQQ157 pKa = 2.64

Molecular weight: 16.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M1SL05|A0A6M1SL05_9RHIZ Pseudoazurin OS=Devosia sp. H239 OX=2714858 GN=G5575_05500 PE=4 SV=1
MM1 pKa = 7.33SAMPRR6 pKa = 11.84HH7 pKa = 6.19IPSTGASAGRR17 pKa = 11.84TLAVASTSEE26 pKa = 4.0IILRR30 pKa = 11.84PRR32 pKa = 11.84EE33 pKa = 4.07VILTFDD39 pKa = 4.07DD40 pKa = 4.55GPRR43 pKa = 11.84AGKK46 pKa = 8.2TPAILDD52 pKa = 3.61TLEE55 pKa = 4.3EE56 pKa = 4.38YY57 pKa = 10.54GVKK60 pKa = 9.39ATFLMLGSAAKK71 pKa = 10.38ANPKK75 pKa = 9.49LARR78 pKa = 11.84EE79 pKa = 4.13VAEE82 pKa = 4.54RR83 pKa = 11.84GHH85 pKa = 5.88TVGSHH90 pKa = 5.79TYY92 pKa = 11.07DD93 pKa = 4.27HH94 pKa = 7.36IDD96 pKa = 3.48LNTVSRR102 pKa = 11.84QEE104 pKa = 3.98ALNEE108 pKa = 3.88IARR111 pKa = 11.84GEE113 pKa = 4.16QAVADD118 pKa = 4.16ALGGAGQALSPFFRR132 pKa = 11.84FPYY135 pKa = 10.16LSQTGFCAPTFSPAISWSSMSISTARR161 pKa = 11.84TITKK165 pKa = 7.73TAPRR169 pKa = 11.84PWRR172 pKa = 11.84RR173 pKa = 11.84EE174 pKa = 3.32RR175 pKa = 11.84WIGSMPVAAASSSSTTFISARR196 pKa = 11.84STCCRR201 pKa = 11.84AFWPSLRR208 pKa = 11.84RR209 pKa = 11.84VAIRR213 pKa = 11.84SSGSRR218 pKa = 11.84PRR220 pKa = 11.84TWVYY224 pKa = 10.23STATT228 pKa = 3.35

Molecular weight: 24.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3157

0

3157

868461

26

4307

275.1

29.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.132 ± 0.062

0.681 ± 0.014

5.858 ± 0.042

5.726 ± 0.04

3.8 ± 0.034

8.403 ± 0.04

1.944 ± 0.024

5.447 ± 0.032

3.12 ± 0.04

10.156 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.025

2.894 ± 0.027

5.002 ± 0.033

3.363 ± 0.024

6.539 ± 0.047

5.678 ± 0.038

5.618 ± 0.034

7.564 ± 0.034

1.322 ± 0.016

2.225 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski