Arthrospira platensis (strain NIES-39 / IAM M-135) (Spirulina platensis)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Microcoleaceae; Arthrospira; Arthrospira platensis

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6009 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4ZPZ7|D4ZPZ7_ARTPN Methyltransferase OS=Arthrospira platensis (strain NIES-39 / IAM M-135) OX=696747 GN=NIES39_Q01180 PE=3 SV=1
MM1 pKa = 6.9NTLNVAQPYY10 pKa = 7.75EE11 pKa = 4.24IPSNEE16 pKa = 3.52NDD18 pKa = 3.31VFLARR23 pKa = 11.84YY24 pKa = 8.71EE25 pKa = 4.15PDD27 pKa = 3.38GSQVWTTRR35 pKa = 11.84LASNRR40 pKa = 11.84SEE42 pKa = 3.85IAHH45 pKa = 6.84ALTTGLDD52 pKa = 3.38GSIYY56 pKa = 10.46VAGFTSGDD64 pKa = 3.25LGGEE68 pKa = 4.12INNGGTDD75 pKa = 3.52AFISNYY81 pKa = 9.92EE82 pKa = 3.8PDD84 pKa = 3.55GTLTRR89 pKa = 11.84TRR91 pKa = 11.84LLGTSNWDD99 pKa = 3.09GARR102 pKa = 11.84ALTTGFDD109 pKa = 3.48GSIYY113 pKa = 9.8MAGEE117 pKa = 3.73TWGDD121 pKa = 3.45LNGEE125 pKa = 4.32INQSGADD132 pKa = 3.7GFIAKK137 pKa = 9.74YY138 pKa = 10.07EE139 pKa = 4.12PDD141 pKa = 3.41GTLVWTRR148 pKa = 11.84LLGSNGWGAAHH159 pKa = 7.73ALTTGIDD166 pKa = 3.41GFIYY170 pKa = 10.6VAGLTEE176 pKa = 4.12GDD178 pKa = 3.29INEE181 pKa = 4.78EE182 pKa = 3.45IFNRR186 pKa = 11.84NRR188 pKa = 11.84GGNALISKK196 pKa = 8.73YY197 pKa = 9.65EE198 pKa = 4.14PEE200 pKa = 5.41DD201 pKa = 3.46GTLVWTTQLGTPNPDD216 pKa = 2.95EE217 pKa = 5.2ANALTTGLDD226 pKa = 3.35GSIYY230 pKa = 9.0MAGWTQGNLGGEE242 pKa = 4.32MNRR245 pKa = 11.84GYY247 pKa = 11.09SDD249 pKa = 3.75GFIAKK254 pKa = 10.04YY255 pKa = 8.38EE256 pKa = 4.16TDD258 pKa = 4.33GALAWTRR265 pKa = 11.84LLASNDD271 pKa = 2.96WDD273 pKa = 3.73GANALTTRR281 pKa = 11.84IDD283 pKa = 3.38GSIYY287 pKa = 9.09IAGYY291 pKa = 8.17TRR293 pKa = 11.84GNLNGEE299 pKa = 4.32INNGASDD306 pKa = 3.77AFMSRR311 pKa = 11.84VIVDD315 pKa = 3.48GTVAEE320 pKa = 4.39PVPPPEE326 pKa = 5.44FIDD329 pKa = 4.2PLLPLNGDD337 pKa = 4.42YY338 pKa = 10.83IPIAGDD344 pKa = 3.57FNGSGITDD352 pKa = 3.87ILWYY356 pKa = 10.61APGPDD361 pKa = 4.95PDD363 pKa = 4.21YY364 pKa = 10.37MWYY367 pKa = 9.75FHH369 pKa = 7.39ADD371 pKa = 2.86GSYY374 pKa = 10.3GSRR377 pKa = 11.84LFMINGYY384 pKa = 8.57YY385 pKa = 9.81IPIAGDD391 pKa = 3.61FNGSGITDD399 pKa = 3.87ILWYY403 pKa = 10.61APGPDD408 pKa = 4.87PDD410 pKa = 4.13FMWYY414 pKa = 10.32FNQDD418 pKa = 2.35GNYY421 pKa = 10.54DD422 pKa = 4.11GFQLTVDD429 pKa = 3.43GHH431 pKa = 5.41YY432 pKa = 11.13APVPADD438 pKa = 3.38LEE440 pKa = 4.66VFF442 pKa = 3.88

Molecular weight: 47.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4ZQM7|D4ZQM7_ARTPN Reverse transcriptase OS=Arthrospira platensis (strain NIES-39 / IAM M-135) OX=696747 GN=NIES39_D07940 PE=4 SV=1
MM1 pKa = 7.42AQQTLKK7 pKa = 9.97GTSRR11 pKa = 11.84KK12 pKa = 9.14KK13 pKa = 10.39KK14 pKa = 8.02RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84NGRR29 pKa = 11.84AVIRR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84QKK38 pKa = 9.97GRR40 pKa = 11.84SRR42 pKa = 11.84LSVV45 pKa = 3.01

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6009

0

6009

1745523

29

4747

290.5

32.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.379 ± 0.034

1.064 ± 0.015

5.262 ± 0.033

6.288 ± 0.04

3.807 ± 0.025

6.836 ± 0.04

1.943 ± 0.017

6.901 ± 0.034

4.511 ± 0.041

10.539 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.984 ± 0.016

4.514 ± 0.031

5.062 ± 0.032

5.299 ± 0.035

5.494 ± 0.028

6.531 ± 0.058

5.652 ± 0.036

6.221 ± 0.033

1.565 ± 0.017

3.15 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski