Hubei tombus-like virus 7

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGP4|A0A1L3KGP4_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 7 OX=1923294 PE=4 SV=1
MM1 pKa = 7.35SFAYY5 pKa = 9.28TYY7 pKa = 9.72FFQPKK12 pKa = 9.23RR13 pKa = 11.84APTFDD18 pKa = 3.64FSTTRR23 pKa = 11.84RR24 pKa = 11.84LDD26 pKa = 3.36AFNPATALTGGPGPNIAPSAEE47 pKa = 3.97GLDD50 pKa = 4.01RR51 pKa = 11.84LPSDD55 pKa = 3.46NAQHH59 pKa = 6.72FEE61 pKa = 4.1QAPSPSVQGEE71 pKa = 4.52GVHH74 pKa = 6.16NAPAVGGAAGVGLLGNGAAHH94 pKa = 7.23AGKK97 pKa = 8.58TDD99 pKa = 3.72AEE101 pKa = 4.35CAAAVIGGSSFEE113 pKa = 4.05LRR115 pKa = 11.84FDD117 pKa = 3.83AFVALCSRR125 pKa = 11.84LRR127 pKa = 11.84PEE129 pKa = 4.75PISRR133 pKa = 11.84AVCEE137 pKa = 4.0FRR139 pKa = 11.84LLNGGLNLDD148 pKa = 3.99EE149 pKa = 4.36RR150 pKa = 11.84VAMARR155 pKa = 11.84CVEE158 pKa = 4.18YY159 pKa = 10.77VDD161 pKa = 4.53QYY163 pKa = 11.11EE164 pKa = 4.44FRR166 pKa = 11.84CPTIAVGALTWDD178 pKa = 3.89DD179 pKa = 4.02LVEE182 pKa = 4.28EE183 pKa = 4.34LASTVRR189 pKa = 11.84ADD191 pKa = 4.47PIEE194 pKa = 4.68GPLNQLRR201 pKa = 11.84PVRR204 pKa = 11.84PRR206 pKa = 11.84PSSPPTAVAAAIPVAVPAPEE226 pKa = 4.31LRR228 pKa = 11.84PISWAAASGRR238 pKa = 11.84AEE240 pKa = 3.95PKK242 pKa = 9.94QLQFAADD249 pKa = 4.24DD250 pKa = 4.12DD251 pKa = 4.54CVPDD255 pKa = 3.71VAHH258 pKa = 7.56DD259 pKa = 3.41SGIRR263 pKa = 11.84VGFVDD268 pKa = 4.92EE269 pKa = 4.45PQVAYY274 pKa = 10.44RR275 pKa = 11.84PADD278 pKa = 3.22SDD280 pKa = 3.99YY281 pKa = 11.55DD282 pKa = 4.19DD283 pKa = 4.98EE284 pKa = 5.32PEE286 pKa = 4.21PSNPIVAPSKK296 pKa = 10.28PQMLSQQRR304 pKa = 11.84GFFWRR309 pKa = 11.84MLDD312 pKa = 3.36SVLPRR317 pKa = 11.84FTIFRR322 pKa = 11.84QAATQLHH329 pKa = 6.11ADD331 pKa = 3.73EE332 pKa = 5.04VKK334 pKa = 10.69VSFRR338 pKa = 11.84NASVDD343 pKa = 3.67DD344 pKa = 4.21EE345 pKa = 4.82PEE347 pKa = 4.59LGEE350 pKa = 4.29VAQHH354 pKa = 6.79RR355 pKa = 11.84FRR357 pKa = 11.84DD358 pKa = 3.78VALGAVSHH366 pKa = 6.58RR367 pKa = 11.84KK368 pKa = 6.84YY369 pKa = 10.56HH370 pKa = 6.02RR371 pKa = 11.84AANRR375 pKa = 11.84WHH377 pKa = 5.46QRR379 pKa = 11.84LRR381 pKa = 11.84LLRR384 pKa = 11.84QLRR387 pKa = 11.84EE388 pKa = 3.76EE389 pKa = 5.06LIFEE393 pKa = 4.07SSEE396 pKa = 4.02VKK398 pKa = 10.08RR399 pKa = 11.84VRR401 pKa = 11.84TDD403 pKa = 3.96ANVAWIAIGARR414 pKa = 11.84KK415 pKa = 9.1LVAKK419 pKa = 9.96QVEE422 pKa = 4.17EE423 pKa = 4.18GKK425 pKa = 9.92IDD427 pKa = 3.46RR428 pKa = 11.84RR429 pKa = 11.84HH430 pKa = 5.57ARR432 pKa = 11.84WFRR435 pKa = 11.84SALTEE440 pKa = 4.17TFFLKK445 pKa = 10.67DD446 pKa = 3.72DD447 pKa = 4.51DD448 pKa = 5.47DD449 pKa = 4.78EE450 pKa = 5.22FVDD453 pKa = 3.65TLRR456 pKa = 11.84GSFARR461 pKa = 11.84AWW463 pKa = 3.33

Molecular weight: 51.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGI0|A0A1L3KGI0_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 7 OX=1923294 PE=4 SV=1
MM1 pKa = 7.78PMGGLWSATPVLPSVRR17 pKa = 11.84TPWRR21 pKa = 11.84WEE23 pKa = 3.66TLLNCTSSGFTASVLLGCHH42 pKa = 4.74STGTCRR48 pKa = 11.84CFVLCIPACEE58 pKa = 4.36SVGSRR63 pKa = 11.84VGLASPIRR71 pKa = 11.84LGTPVLCNSRR81 pKa = 11.84YY82 pKa = 9.89ARR84 pKa = 11.84QALLLSCLPDD94 pKa = 3.89LLLTNAAYY102 pKa = 10.01HH103 pKa = 6.3LPRR106 pKa = 11.84RR107 pKa = 11.84SISAPHH113 pKa = 6.47CRR115 pKa = 11.84PFWRR119 pKa = 11.84PSSFVLTLTVSLMQTSAQSSRR140 pKa = 11.84FWPDD144 pKa = 2.62HH145 pKa = 6.4RR146 pKa = 11.84GMPRR150 pKa = 11.84NAKK153 pKa = 7.91TANPPASKK161 pKa = 10.34GGQSGRR167 pKa = 11.84QTTPSSRR174 pKa = 11.84PRR176 pKa = 11.84GGTAQKK182 pKa = 10.46VRR184 pKa = 11.84GKK186 pKa = 10.93GEE188 pKa = 3.88YY189 pKa = 10.3LLPGTFAHH197 pKa = 6.8VGRR200 pKa = 11.84KK201 pKa = 9.44LGEE204 pKa = 4.12KK205 pKa = 10.3AGGWLGGGAGALLSTVTGMGDD226 pKa = 3.35YY227 pKa = 10.94QMNNIMHH234 pKa = 7.91ADD236 pKa = 3.86RR237 pKa = 11.84ALTSAHH243 pKa = 6.01PKK245 pKa = 10.26SYY247 pKa = 10.8EE248 pKa = 3.83FTNTEE253 pKa = 3.88LVQVVASTGEE263 pKa = 3.93AFQSYY268 pKa = 8.91QYY270 pKa = 11.51ANNPGLGNFPWLSAIAQRR288 pKa = 11.84FNKK291 pKa = 9.79YY292 pKa = 8.84RR293 pKa = 11.84FKK295 pKa = 10.72QLVYY299 pKa = 9.88HH300 pKa = 6.38FEE302 pKa = 4.45STSSEE307 pKa = 4.2YY308 pKa = 10.95AAGAGLGTVAIATNYY323 pKa = 10.17DD324 pKa = 3.14AVDD327 pKa = 3.49RR328 pKa = 11.84EE329 pKa = 4.23FASMVEE335 pKa = 4.22MEE337 pKa = 4.17ATEE340 pKa = 4.07NAVSGKK346 pKa = 9.78PSVDD350 pKa = 2.92KK351 pKa = 11.37LHH353 pKa = 6.66GVEE356 pKa = 5.16CATAEE361 pKa = 4.19NALKK365 pKa = 8.99WWYY368 pKa = 9.79VRR370 pKa = 11.84SGPPPASTDD379 pKa = 2.8LRR381 pKa = 11.84MYY383 pKa = 11.26DD384 pKa = 3.31MSEE387 pKa = 3.87TTLATEE393 pKa = 4.85GLFASPGTVIGRR405 pKa = 11.84LKK407 pKa = 10.77VFYY410 pKa = 9.73TVEE413 pKa = 4.04FCNPIALGIPAPYY426 pKa = 10.24LPAPVTLRR434 pKa = 11.84SWYY437 pKa = 10.64APGAAAAGVGPYY449 pKa = 10.03FGLKK453 pKa = 10.37DD454 pKa = 4.16SISSPTWSVGPYY466 pKa = 7.74QTLPVNVSGGSVGSIGVTTYY486 pKa = 10.68PVVDD490 pKa = 3.56VTTNTLKK497 pKa = 10.62FYY499 pKa = 11.02CRR501 pKa = 11.84GTFYY505 pKa = 10.14VTARR509 pKa = 11.84FFFSTAPTVTTIAQSTSGACTLNNPYY535 pKa = 10.47SAGNTWHH542 pKa = 6.07VTTSTDD548 pKa = 2.97KK549 pKa = 10.85TLLSVEE555 pKa = 4.14GVITVTSASYY565 pKa = 7.34TTPASVSLSQSGWGSTFVIGSALTSVSYY593 pKa = 10.86VGQPP597 pKa = 2.88

Molecular weight: 63.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1501

441

597

500.3

54.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.593 ± 1.089

1.799 ± 0.211

4.863 ± 1.363

5.263 ± 0.98

4.664 ± 0.427

7.662 ± 0.91

1.799 ± 0.13

2.665 ± 0.165

3.131 ± 0.467

8.794 ± 0.634

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.865 ± 0.412

3.131 ± 0.221

6.063 ± 0.96

3.464 ± 0.37

7.395 ± 1.205

7.861 ± 1.27

6.262 ± 1.865

7.861 ± 0.103

1.865 ± 0.162

2.998 ± 0.599
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski