Amino acid dipepetide frequency for Frondihabitans sp. PhB188

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.708AlaAla: 19.708 ± 0.18
0.611AlaCys: 0.611 ± 0.024
8.274AlaAsp: 8.274 ± 0.095
7.096AlaGlu: 7.096 ± 0.096
4.03AlaPhe: 4.03 ± 0.062
12.161AlaGly: 12.161 ± 0.111
2.272AlaHis: 2.272 ± 0.046
5.652AlaIle: 5.652 ± 0.075
3.116AlaLys: 3.116 ± 0.062
13.583AlaLeu: 13.583 ± 0.137
2.563AlaMet: 2.563 ± 0.051
2.344AlaAsn: 2.344 ± 0.057
6.576AlaPro: 6.576 ± 0.112
3.614AlaGln: 3.614 ± 0.059
8.285AlaArg: 8.285 ± 0.106
8.111AlaSer: 8.111 ± 0.092
8.351AlaThr: 8.351 ± 0.098
11.872AlaVal: 11.872 ± 0.121
1.721AlaTrp: 1.721 ± 0.05
2.404AlaTyr: 2.404 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.569CysAla: 0.569 ± 0.026
0.039CysCys: 0.039 ± 0.006
0.287CysAsp: 0.287 ± 0.016
0.212CysGlu: 0.212 ± 0.014
0.149CysPhe: 0.149 ± 0.012
0.489CysGly: 0.489 ± 0.022
0.104CysHis: 0.104 ± 0.01
0.167CysIle: 0.167 ± 0.014
0.053CysLys: 0.053 ± 0.007
0.47CysLeu: 0.47 ± 0.025
0.064CysMet: 0.064 ± 0.008
0.088CysAsn: 0.088 ± 0.01
0.248CysPro: 0.248 ± 0.018
0.118CysGln: 0.118 ± 0.011
0.271CysArg: 0.271 ± 0.015
0.334CysSer: 0.334 ± 0.016
0.295CysThr: 0.295 ± 0.019
0.411CysVal: 0.411 ± 0.018
0.05CysTrp: 0.05 ± 0.008
0.1CysTyr: 0.1 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
8.093AspAla: 8.093 ± 0.084
0.234AspCys: 0.234 ± 0.016
4.719AspAsp: 4.719 ± 0.087
4.216AspGlu: 4.216 ± 0.066
1.811AspPhe: 1.811 ± 0.045
6.031AspGly: 6.031 ± 0.09
1.225AspHis: 1.225 ± 0.034
2.323AspIle: 2.323 ± 0.046
1.105AspLys: 1.105 ± 0.038
6.415AspLeu: 6.415 ± 0.083
0.745AspMet: 0.745 ± 0.025
1.009AspAsn: 1.009 ± 0.038
3.824AspPro: 3.824 ± 0.051
1.654AspGln: 1.654 ± 0.039
4.336AspArg: 4.336 ± 0.075
3.329AspSer: 3.329 ± 0.06
3.201AspThr: 3.201 ± 0.056
5.492AspVal: 5.492 ± 0.069
0.933AspTrp: 0.933 ± 0.032
1.307AspTyr: 1.307 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
6.823GluAla: 6.823 ± 0.094
0.183GluCys: 0.183 ± 0.015
1.898GluAsp: 1.898 ± 0.047
2.244GluGlu: 2.244 ± 0.054
1.818GluPhe: 1.818 ± 0.045
3.638GluGly: 3.638 ± 0.058
1.345GluHis: 1.345 ± 0.048
2.601GluIle: 2.601 ± 0.052
1.539GluLys: 1.539 ± 0.041
5.94GluLeu: 5.94 ± 0.084
0.938GluMet: 0.938 ± 0.028
1.147GluAsn: 1.147 ± 0.037
2.845GluPro: 2.845 ± 0.054
1.918GluGln: 1.918 ± 0.046
4.349GluArg: 4.349 ± 0.075
3.133GluSer: 3.133 ± 0.056
3.09GluThr: 3.09 ± 0.048
4.346GluVal: 4.346 ± 0.062
0.819GluTrp: 0.819 ± 0.027
1.037GluTyr: 1.037 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.237PheAla: 4.237 ± 0.074
0.179PheCys: 0.179 ± 0.013
2.655PheAsp: 2.655 ± 0.054
1.777PheGlu: 1.777 ± 0.045
1.12PhePhe: 1.12 ± 0.032
3.541PheGly: 3.541 ± 0.065
0.631PheHis: 0.631 ± 0.022
1.155PheIle: 1.155 ± 0.038
0.522PheLys: 0.522 ± 0.023
2.975PheLeu: 2.975 ± 0.059
0.428PheMet: 0.428 ± 0.02
0.631PheAsn: 0.631 ± 0.024
1.369PhePro: 1.369 ± 0.031
0.852PheGln: 0.852 ± 0.031
1.781PheArg: 1.781 ± 0.041
1.921PheSer: 1.921 ± 0.038
2.251PheThr: 2.251 ± 0.052
3.05PheVal: 3.05 ± 0.057
0.483PheTrp: 0.483 ± 0.019
0.694PheTyr: 0.694 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
10.644GlyAla: 10.644 ± 0.106
0.46GlyCys: 0.46 ± 0.023
5.19GlyAsp: 5.19 ± 0.078
4.337GlyGlu: 4.337 ± 0.063
3.394GlyPhe: 3.394 ± 0.058
7.719GlyGly: 7.719 ± 0.106
1.887GlyHis: 1.887 ± 0.04
4.655GlyIle: 4.655 ± 0.064
2.184GlyLys: 2.184 ± 0.053
9.076GlyLeu: 9.076 ± 0.097
1.69GlyMet: 1.69 ± 0.043
1.712GlyAsn: 1.712 ± 0.045
3.706GlyPro: 3.706 ± 0.069
2.477GlyGln: 2.477 ± 0.055
6.329GlyArg: 6.329 ± 0.092
6.345GlySer: 6.345 ± 0.083
6.235GlyThr: 6.235 ± 0.104
8.147GlyVal: 8.147 ± 0.093
1.47GlyTrp: 1.47 ± 0.035
2.26GlyTyr: 2.26 ± 0.04
0.0GlyXaa: 0.0 ± 0.0
His
2.214HisAla: 2.214 ± 0.048
0.079HisCys: 0.079 ± 0.009
1.503HisAsp: 1.503 ± 0.036
1.107HisGlu: 1.107 ± 0.031
0.612HisPhe: 0.612 ± 0.025
1.919HisGly: 1.919 ± 0.045
0.508HisHis: 0.508 ± 0.028
0.67HisIle: 0.67 ± 0.023
0.262HisLys: 0.262 ± 0.015
2.078HisLeu: 2.078 ± 0.049
0.254HisMet: 0.254 ± 0.013
0.347HisAsn: 0.347 ± 0.017
1.381HisPro: 1.381 ± 0.035
0.498HisGln: 0.498 ± 0.02
1.522HisArg: 1.522 ± 0.04
1.058HisSer: 1.058 ± 0.035
0.972HisThr: 0.972 ± 0.029
1.698HisVal: 1.698 ± 0.039
0.256HisTrp: 0.256 ± 0.017
0.417HisTyr: 0.417 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.532IleAla: 6.532 ± 0.077
0.223IleCys: 0.223 ± 0.014
3.34IleAsp: 3.34 ± 0.058
2.478IleGlu: 2.478 ± 0.046
1.095IlePhe: 1.095 ± 0.037
4.52IleGly: 4.52 ± 0.071
0.697IleHis: 0.697 ± 0.026
1.701IleIle: 1.701 ± 0.049
0.897IleLys: 0.897 ± 0.034
3.768IleLeu: 3.768 ± 0.07
0.595IleMet: 0.595 ± 0.026
0.895IleAsn: 0.895 ± 0.031
2.212IlePro: 2.212 ± 0.05
1.055IleGln: 1.055 ± 0.033
2.651IleArg: 2.651 ± 0.047
2.52IleSer: 2.52 ± 0.044
3.033IleThr: 3.033 ± 0.064
4.703IleVal: 4.703 ± 0.075
0.459IleTrp: 0.459 ± 0.022
0.692IleTyr: 0.692 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
3.173LysAla: 3.173 ± 0.061
0.078LysCys: 0.078 ± 0.009
1.112LysAsp: 1.112 ± 0.033
0.894LysGlu: 0.894 ± 0.026
0.579LysPhe: 0.579 ± 0.023
1.77LysGly: 1.77 ± 0.046
0.51LysHis: 0.51 ± 0.022
1.004LysIle: 1.004 ± 0.036
1.058LysLys: 1.058 ± 0.043
2.04LysLeu: 2.04 ± 0.046
0.429LysMet: 0.429 ± 0.022
0.708LysAsn: 0.708 ± 0.024
1.392LysPro: 1.392 ± 0.035
0.828LysGln: 0.828 ± 0.03
1.63LysArg: 1.63 ± 0.044
1.541LysSer: 1.541 ± 0.046
1.672LysThr: 1.672 ± 0.045
1.874LysVal: 1.874 ± 0.047
0.271LysTrp: 0.271 ± 0.018
0.533LysTyr: 0.533 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
14.106LeuAla: 14.106 ± 0.145
0.445LeuCys: 0.445 ± 0.019
6.648LeuAsp: 6.648 ± 0.082
5.039LeuGlu: 5.039 ± 0.084
3.023LeuPhe: 3.023 ± 0.059
9.323LeuGly: 9.323 ± 0.099
1.846LeuHis: 1.846 ± 0.044
4.259LeuIle: 4.259 ± 0.067
2.016LeuLys: 2.016 ± 0.052
9.863LeuLeu: 9.863 ± 0.138
1.59LeuMet: 1.59 ± 0.038
1.756LeuAsn: 1.756 ± 0.048
5.41LeuPro: 5.41 ± 0.074
2.46LeuGln: 2.46 ± 0.047
6.982LeuArg: 6.982 ± 0.101
6.061LeuSer: 6.061 ± 0.097
6.45LeuThr: 6.45 ± 0.079
9.474LeuVal: 9.474 ± 0.104
1.164LeuTrp: 1.164 ± 0.036
1.683LeuTyr: 1.683 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
2.095MetAla: 2.095 ± 0.041
0.086MetCys: 0.086 ± 0.007
0.706MetAsp: 0.706 ± 0.025
0.61MetGlu: 0.61 ± 0.023
0.493MetPhe: 0.493 ± 0.021
1.177MetGly: 1.177 ± 0.032
0.321MetHis: 0.321 ± 0.016
0.873MetIle: 0.873 ± 0.029
0.49MetLys: 0.49 ± 0.021
1.667MetLeu: 1.667 ± 0.041
0.306MetMet: 0.306 ± 0.018
0.462MetAsn: 0.462 ± 0.023
1.063MetPro: 1.063 ± 0.03
0.473MetGln: 0.473 ± 0.022
1.224MetArg: 1.224 ± 0.033
1.48MetSer: 1.48 ± 0.04
1.818MetThr: 1.818 ± 0.034
1.238MetVal: 1.238 ± 0.036
0.156MetTrp: 0.156 ± 0.012
0.253MetTyr: 0.253 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.426AsnAla: 2.426 ± 0.054
0.117AsnCys: 0.117 ± 0.01
1.216AsnAsp: 1.216 ± 0.033
0.927AsnGlu: 0.927 ± 0.032
0.683AsnPhe: 0.683 ± 0.026
2.0AsnGly: 2.0 ± 0.063
0.353AsnHis: 0.353 ± 0.018
0.811AsnIle: 0.811 ± 0.03
0.441AsnLys: 0.441 ± 0.022
1.856AsnLeu: 1.856 ± 0.043
0.338AsnMet: 0.338 ± 0.019
0.516AsnAsn: 0.516 ± 0.023
1.47AsnPro: 1.47 ± 0.034
0.591AsnGln: 0.591 ± 0.025
1.295AsnArg: 1.295 ± 0.035
1.187AsnSer: 1.187 ± 0.038
1.205AsnThr: 1.205 ± 0.036
1.755AsnVal: 1.755 ± 0.043
0.324AsnTrp: 0.324 ± 0.016
0.501AsnTyr: 0.501 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
7.288ProAla: 7.288 ± 0.127
0.154ProCys: 0.154 ± 0.013
3.677ProAsp: 3.677 ± 0.064
3.393ProGlu: 3.393 ± 0.059
1.754ProPhe: 1.754 ± 0.038
5.013ProGly: 5.013 ± 0.077
1.053ProHis: 1.053 ± 0.03
2.052ProIle: 2.052 ± 0.041
1.207ProLys: 1.207 ± 0.038
4.781ProLeu: 4.781 ± 0.08
0.848ProMet: 0.848 ± 0.026
1.043ProAsn: 1.043 ± 0.029
2.066ProPro: 2.066 ± 0.045
1.396ProGln: 1.396 ± 0.036
3.223ProArg: 3.223 ± 0.06
3.607ProSer: 3.607 ± 0.057
3.659ProThr: 3.659 ± 0.062
4.915ProVal: 4.915 ± 0.077
0.794ProTrp: 0.794 ± 0.03
0.957ProTyr: 0.957 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.486GlnAla: 3.486 ± 0.057
0.098GlnCys: 0.098 ± 0.01
1.181GlnAsp: 1.181 ± 0.03
1.332GlnGlu: 1.332 ± 0.032
0.878GlnPhe: 0.878 ± 0.03
2.179GlnGly: 2.179 ± 0.046
0.582GlnHis: 0.582 ± 0.024
1.391GlnIle: 1.391 ± 0.037
0.841GlnLys: 0.841 ± 0.028
2.967GlnLeu: 2.967 ± 0.056
0.509GlnMet: 0.509 ± 0.02
0.695GlnAsn: 0.695 ± 0.031
1.464GlnPro: 1.464 ± 0.041
1.162GlnGln: 1.162 ± 0.041
2.058GlnArg: 2.058 ± 0.042
1.661GlnSer: 1.661 ± 0.045
1.76GlnThr: 1.76 ± 0.042
2.329GlnVal: 2.329 ± 0.043
0.4GlnTrp: 0.4 ± 0.022
0.663GlnTyr: 0.663 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
8.233ArgAla: 8.233 ± 0.108
0.273ArgCys: 0.273 ± 0.019
4.191ArgAsp: 4.191 ± 0.064
3.756ArgGlu: 3.756 ± 0.069
2.371ArgPhe: 2.371 ± 0.05
5.101ArgGly: 5.101 ± 0.071
1.465ArgHis: 1.465 ± 0.04
3.392ArgIle: 3.392 ± 0.054
1.341ArgLys: 1.341 ± 0.037
7.136ArgLeu: 7.136 ± 0.093
1.455ArgMet: 1.455 ± 0.034
1.29ArgAsn: 1.29 ± 0.04
3.621ArgPro: 3.621 ± 0.058
2.036ArgGln: 2.036 ± 0.046
6.046ArgArg: 6.046 ± 0.095
4.252ArgSer: 4.252 ± 0.069
4.065ArgThr: 4.065 ± 0.069
5.847ArgVal: 5.847 ± 0.081
1.019ArgTrp: 1.019 ± 0.033
1.439ArgTyr: 1.439 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
7.851SerAla: 7.851 ± 0.088
0.319SerCys: 0.319 ± 0.017
3.67SerAsp: 3.67 ± 0.058
2.784SerGlu: 2.784 ± 0.054
2.159SerPhe: 2.159 ± 0.045
6.35SerGly: 6.35 ± 0.09
1.144SerHis: 1.144 ± 0.036
2.904SerIle: 2.904 ± 0.055
1.628SerLys: 1.628 ± 0.047
5.912SerLeu: 5.912 ± 0.07
1.251SerMet: 1.251 ± 0.031
1.348SerAsn: 1.348 ± 0.035
3.406SerPro: 3.406 ± 0.06
1.595SerGln: 1.595 ± 0.034
4.169SerArg: 4.169 ± 0.073
4.752SerSer: 4.752 ± 0.076
4.831SerThr: 4.831 ± 0.075
5.206SerVal: 5.206 ± 0.074
0.992SerTrp: 0.992 ± 0.032
1.316SerTyr: 1.316 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
8.665ThrAla: 8.665 ± 0.114
0.289ThrCys: 0.289 ± 0.017
3.587ThrAsp: 3.587 ± 0.058
2.966ThrGlu: 2.966 ± 0.053
2.008ThrPhe: 2.008 ± 0.05
6.337ThrGly: 6.337 ± 0.094
1.185ThrHis: 1.185 ± 0.028
3.027ThrIle: 3.027 ± 0.061
1.585ThrLys: 1.585 ± 0.042
6.234ThrLeu: 6.234 ± 0.082
1.023ThrMet: 1.023 ± 0.031
1.322ThrAsn: 1.322 ± 0.036
4.294ThrPro: 4.294 ± 0.067
1.584ThrGln: 1.584 ± 0.043
3.952ThrArg: 3.952 ± 0.059
4.341ThrSer: 4.341 ± 0.061
5.14ThrThr: 5.14 ± 0.085
6.499ThrVal: 6.499 ± 0.116
0.966ThrTrp: 0.966 ± 0.026
1.405ThrTyr: 1.405 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
12.224ValAla: 12.224 ± 0.128
0.444ValCys: 0.444 ± 0.021
6.105ValAsp: 6.105 ± 0.076
4.881ValGlu: 4.881 ± 0.077
2.983ValPhe: 2.983 ± 0.053
7.642ValGly: 7.642 ± 0.1
1.642ValHis: 1.642 ± 0.042
4.143ValIle: 4.143 ± 0.066
1.992ValLys: 1.992 ± 0.046
9.168ValLeu: 9.168 ± 0.109
1.413ValMet: 1.413 ± 0.035
1.748ValAsn: 1.748 ± 0.042
4.797ValPro: 4.797 ± 0.072
2.194ValGln: 2.194 ± 0.043
5.634ValArg: 5.634 ± 0.081
5.627ValSer: 5.627 ± 0.084
6.233ValThr: 6.233 ± 0.109
9.696ValVal: 9.696 ± 0.128
1.104ValTrp: 1.104 ± 0.036
1.633ValTyr: 1.633 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.594TrpAla: 1.594 ± 0.035
0.083TrpCys: 0.083 ± 0.008
0.622TrpAsp: 0.622 ± 0.025
0.532TrpGlu: 0.532 ± 0.022
0.552TrpPhe: 0.552 ± 0.022
1.029TrpGly: 1.029 ± 0.031
0.3TrpHis: 0.3 ± 0.015
0.639TrpIle: 0.639 ± 0.022
0.369TrpLys: 0.369 ± 0.019
1.584TrpLeu: 1.584 ± 0.044
0.31TrpMet: 0.31 ± 0.015
0.438TrpAsn: 0.438 ± 0.02
0.7TrpPro: 0.7 ± 0.025
0.53TrpGln: 0.53 ± 0.024
1.096TrpArg: 1.096 ± 0.032
1.076TrpSer: 1.076 ± 0.038
0.892TrpThr: 0.892 ± 0.03
1.056TrpVal: 1.056 ± 0.031
0.31TrpTrp: 0.31 ± 0.016
0.277TrpTyr: 0.277 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.383TyrAla: 2.383 ± 0.044
0.124TyrCys: 0.124 ± 0.011
1.422TyrAsp: 1.422 ± 0.041
1.07TyrGlu: 1.07 ± 0.03
0.742TyrPhe: 0.742 ± 0.027
1.977TyrGly: 1.977 ± 0.047
0.283TyrHis: 0.283 ± 0.016
0.647TyrIle: 0.647 ± 0.026
0.448TyrLys: 0.448 ± 0.023
2.088TyrLeu: 2.088 ± 0.04
0.239TyrMet: 0.239 ± 0.015
0.511TyrAsn: 0.511 ± 0.022
1.034TyrPro: 1.034 ± 0.03
0.599TyrGln: 0.599 ± 0.025
1.495TyrArg: 1.495 ± 0.041
1.253TyrSer: 1.253 ± 0.032
1.259TyrThr: 1.259 ± 0.044
1.698TyrVal: 1.698 ± 0.04
0.297TyrTrp: 0.297 ± 0.016
0.501TyrTyr: 0.501 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3539 proteins (1122767 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski