Tick-associated genomovirus 2
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D0Y767|A0A2D0Y767_9VIRU Capsid protein OS=Tick-associated genomovirus 2 OX=2025478 PE=4 SV=1
MM1 pKa = 6.83 PAKK4 pKa = 10.37 YY5 pKa = 10.16 KK6 pKa = 10.83 LDD8 pKa = 3.76 GEE10 pKa = 4.52 QFFMLTYY17 pKa = 7.49 PTTPADD23 pKa = 3.72 FDD25 pKa = 4.2 GSGIIAILEE34 pKa = 3.9 RR35 pKa = 11.84 LGCNYY40 pKa = 10.01 RR41 pKa = 11.84 VGRR44 pKa = 11.84 EE45 pKa = 3.75 LHH47 pKa = 6.22 QDD49 pKa = 3.86 GKK51 pKa = 9.59 PHH53 pKa = 6.0 FHH55 pKa = 7.08 AMCCFDD61 pKa = 4.56 EE62 pKa = 5.53 PYY64 pKa = 10.51 TDD66 pKa = 3.48 GDD68 pKa = 3.27 ARR70 pKa = 11.84 RR71 pKa = 11.84 TFTVGTRR78 pKa = 11.84 IPNIRR83 pKa = 11.84 VRR85 pKa = 11.84 RR86 pKa = 11.84 TRR88 pKa = 11.84 PEE90 pKa = 3.95 RR91 pKa = 11.84 GWDD94 pKa = 3.51 YY95 pKa = 11.19 VGKK98 pKa = 9.63 HH99 pKa = 5.89 AGTKK103 pKa = 9.13 EE104 pKa = 3.65 GHH106 pKa = 6.76 HH107 pKa = 6.11 IVGEE111 pKa = 4.1 KK112 pKa = 8.63 GTRR115 pKa = 11.84 PGGDD119 pKa = 3.31 EE120 pKa = 4.56 DD121 pKa = 4.55 SSEE124 pKa = 4.19 RR125 pKa = 11.84 PSNDD129 pKa = 2.18 SWHH132 pKa = 6.69 EE133 pKa = 3.93 IILARR138 pKa = 11.84 TRR140 pKa = 11.84 EE141 pKa = 4.05 EE142 pKa = 4.16 FFDD145 pKa = 3.77 LAARR149 pKa = 11.84 LAPRR153 pKa = 11.84 QLACSFTSLSAYY165 pKa = 10.31 ADD167 pKa = 3.13 WKK169 pKa = 9.38 YY170 pKa = 11.03 RR171 pKa = 11.84 PVKK174 pKa = 10.14 EE175 pKa = 4.46 EE176 pKa = 4.05 YY177 pKa = 8.66 VTPEE181 pKa = 4.37 GDD183 pKa = 2.63 WSVPFEE189 pKa = 4.18 LQDD192 pKa = 3.37 WVDD195 pKa = 3.82 DD196 pKa = 4.3 NVRR199 pKa = 11.84 GTPGRR204 pKa = 11.84 PKK206 pKa = 10.84 GLVLFGATRR215 pKa = 11.84 LGKK218 pKa = 7.65 TVWARR223 pKa = 11.84 SLGSHH228 pKa = 6.62 YY229 pKa = 10.68 YY230 pKa = 10.71 AGGLWDD236 pKa = 3.64 MAEE239 pKa = 4.13 FHH241 pKa = 6.57 PQEE244 pKa = 3.81 QEE246 pKa = 3.79 YY247 pKa = 10.64 AIWDD251 pKa = 3.83 DD252 pKa = 3.55 MLGGLKK258 pKa = 10.24 AGYY261 pKa = 9.78 FNYY264 pKa = 10.28 KK265 pKa = 9.29 NWLGGQFAFTIQDD278 pKa = 3.08 KK279 pKa = 10.49 YY280 pKa = 11.37 GKK282 pKa = 9.7 KK283 pKa = 10.23 KK284 pKa = 10.01 SIKK287 pKa = 8.46 WGKK290 pKa = 9.46 PSIFICNVDD299 pKa = 3.93 PRR301 pKa = 11.84 DD302 pKa = 3.76 EE303 pKa = 4.4 PPAFNDD309 pKa = 3.36 RR310 pKa = 11.84 VGMDD314 pKa = 3.5 WAWLEE319 pKa = 4.27 EE320 pKa = 3.93 NCVFYY325 pKa = 10.42 EE326 pKa = 4.07 VKK328 pKa = 10.41 EE329 pKa = 4.29 AIFRR333 pKa = 11.84 ASTEE337 pKa = 3.81
Molecular weight: 38.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.643
IPC2_protein 5.639
IPC_protein 5.664
Toseland 5.995
ProMoST 5.855
Dawson 5.881
Bjellqvist 5.919
Wikipedia 5.868
Rodwell 5.855
Grimsley 6.122
Solomon 5.881
Lehninger 5.868
Nozaki 6.122
DTASelect 6.313
Thurlkill 6.287
EMBOSS 6.249
Sillero 6.224
Patrickios 4.151
IPC_peptide 5.893
IPC2_peptide 6.211
IPC2.peptide.svr19 6.222
Protein with the highest isoelectric point:
>tr|A0A2D0Y767|A0A2D0Y767_9VIRU Capsid protein OS=Tick-associated genomovirus 2 OX=2025478 PE=4 SV=1
MM1 pKa = 7.9 AYY3 pKa = 8.65 TRR5 pKa = 11.84 KK6 pKa = 9.66 RR7 pKa = 11.84 RR8 pKa = 11.84 YY9 pKa = 6.82 TRR11 pKa = 11.84 RR12 pKa = 11.84 SYY14 pKa = 11.46 GSSKK18 pKa = 10.65 AKK20 pKa = 9.08 KK21 pKa = 5.11 TTRR24 pKa = 11.84 PSARR28 pKa = 11.84 RR29 pKa = 11.84 YY30 pKa = 9.61 RR31 pKa = 11.84 STGRR35 pKa = 11.84 TRR37 pKa = 11.84 RR38 pKa = 11.84 SIRR41 pKa = 11.84 KK42 pKa = 8.78 APMSKK47 pKa = 9.85 RR48 pKa = 11.84 RR49 pKa = 11.84 ILNTSSRR56 pKa = 11.84 KK57 pKa = 9.32 KK58 pKa = 10.23 RR59 pKa = 11.84 NGMLSWSNTTTSGSVRR75 pKa = 11.84 APAIGPAYY83 pKa = 10.74 VNGAVGGFFAWQATAQQLDD102 pKa = 3.91 AQSTIANQATRR113 pKa = 11.84 TATTCYY119 pKa = 9.1 MKK121 pKa = 10.88 GLAEE125 pKa = 4.26 HH126 pKa = 6.77 LRR128 pKa = 11.84 IQTSSGLPWFHH139 pKa = 6.88 RR140 pKa = 11.84 RR141 pKa = 11.84 VCFTLKK147 pKa = 10.61 GINAFNSALSTDD159 pKa = 3.88 TPNFPISYY167 pKa = 10.37 YY168 pKa = 10.61 VDD170 pKa = 3.1 TSNGIEE176 pKa = 3.89 RR177 pKa = 11.84 LWQNEE182 pKa = 4.22 QINNMPNTLNAQWSLLFKK200 pKa = 10.51 GTVDD204 pKa = 4.75 KK205 pKa = 11.13 DD206 pKa = 3.23 WNDD209 pKa = 3.69 YY210 pKa = 10.58 IIAPIDD216 pKa = 3.48 TTRR219 pKa = 11.84 VSVKK223 pKa = 10.02 FDD225 pKa = 3.5 KK226 pKa = 10.39 TWTMQSGNSSGIVRR240 pKa = 11.84 EE241 pKa = 4.05 RR242 pKa = 11.84 KK243 pKa = 8.79 LWHH246 pKa = 6.56 PMNHH250 pKa = 5.87 NLVYY254 pKa = 10.86 DD255 pKa = 4.19 DD256 pKa = 5.71 DD257 pKa = 4.83 EE258 pKa = 6.49 NGDD261 pKa = 4.11 IEE263 pKa = 4.56 SSSYY267 pKa = 11.64 NSTTSKK273 pKa = 11.16 AGMGDD278 pKa = 3.79 YY279 pKa = 10.85 YY280 pKa = 10.99 VIDD283 pKa = 3.37 MFQRR287 pKa = 11.84 RR288 pKa = 11.84 SRR290 pKa = 11.84 WIKK293 pKa = 10.06 YY294 pKa = 9.24 GSSQITTNSTLYY306 pKa = 9.27 WHH308 pKa = 7.04 EE309 pKa = 4.08 KK310 pKa = 9.32
Molecular weight: 35.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.838
IPC_protein 10.482
Toseland 10.452
ProMoST 10.204
Dawson 10.628
Bjellqvist 10.335
Wikipedia 10.833
Rodwell 10.921
Grimsley 10.701
Solomon 10.672
Lehninger 10.643
Nozaki 10.438
DTASelect 10.321
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.54
Patrickios 10.57
IPC_peptide 10.672
IPC2_peptide 9.18
IPC2.peptide.svr19 8.596
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
886
239
337
295.3
33.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.885 ± 0.112
1.58 ± 0.451
6.433 ± 0.888
5.418 ± 1.488
4.289 ± 0.744
8.465 ± 1.088
2.37 ± 0.351
4.74 ± 0.367
5.192 ± 0.488
6.095 ± 0.708
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.144 ± 0.351
3.837 ± 1.363
5.079 ± 0.857
2.709 ± 0.549
8.691 ± 0.604
6.659 ± 2.01
7.336 ± 1.418
4.515 ± 0.462
3.047 ± 0.333
4.515 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here