Apis mellifera associated microvirus 46

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UUF3|A0A3S8UUF3_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 46 OX=2494776 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 10.3EE3 pKa = 4.07LIKK6 pKa = 10.57KK7 pKa = 8.02GKK9 pKa = 9.43RR10 pKa = 11.84WAEE13 pKa = 3.95KK14 pKa = 8.82LTSSGKK20 pKa = 9.83EE21 pKa = 3.93VLDD24 pKa = 4.09DD25 pKa = 3.9TPIAAPLRR33 pKa = 11.84LTRR36 pKa = 11.84PPSRR40 pKa = 11.84IEE42 pKa = 4.09DD43 pKa = 3.52MRR45 pKa = 11.84LLLSIVEE52 pKa = 4.17RR53 pKa = 11.84QRR55 pKa = 11.84AEE57 pKa = 3.78RR58 pKa = 11.84GHH60 pKa = 6.24EE61 pKa = 4.15SFEE64 pKa = 4.04EE65 pKa = 4.74ADD67 pKa = 4.37DD68 pKa = 4.26FDD70 pKa = 5.56VGDD73 pKa = 4.91DD74 pKa = 3.65YY75 pKa = 11.94DD76 pKa = 3.74PRR78 pKa = 11.84SPHH81 pKa = 6.19EE82 pKa = 3.8LAISHH87 pKa = 6.19EE88 pKa = 4.54LEE90 pKa = 4.08PHH92 pKa = 6.8LSPVDD97 pKa = 3.9EE98 pKa = 5.28DD99 pKa = 4.1PSPASGEE106 pKa = 4.22GSPGAAKK113 pKa = 10.47RR114 pKa = 11.84EE115 pKa = 4.42GGDD118 pKa = 3.73PPADD122 pKa = 3.55SAPPPSKK129 pKa = 10.87AA130 pKa = 3.57

Molecular weight: 14.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UUA3|A0A3S8UUA3_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 46 OX=2494776 PE=3 SV=1
MM1 pKa = 7.46KK2 pKa = 10.28KK3 pKa = 9.97VLSRR7 pKa = 11.84SGRR10 pKa = 11.84RR11 pKa = 11.84VVVINASGSEE21 pKa = 4.06SRR23 pKa = 11.84SASGFSPLLAVPEE36 pKa = 4.17IFRR39 pKa = 11.84SGVRR43 pKa = 11.84SQDD46 pKa = 2.89NFTDD50 pKa = 4.0LDD52 pKa = 4.07LSVPFPVADD61 pKa = 3.12RR62 pKa = 11.84RR63 pKa = 11.84LFNPEE68 pKa = 3.64NPISRR73 pKa = 11.84PAKK76 pKa = 9.18RR77 pKa = 11.84FSGIPARR84 pKa = 11.84VISPKK89 pKa = 10.26LKK91 pKa = 8.3KK92 pKa = 10.23ARR94 pKa = 11.84YY95 pKa = 7.93GRR97 pKa = 11.84SRR99 pKa = 11.84PFLSSFPSVLAFQRR113 pKa = 11.84PRR115 pKa = 11.84SVALCVRR122 pKa = 11.84RR123 pKa = 11.84SVRR126 pKa = 11.84RR127 pKa = 11.84QVLFSRR133 pKa = 11.84GVPGRR138 pKa = 11.84RR139 pKa = 11.84VAKK142 pKa = 9.62PGRR145 pKa = 11.84NAFSNVRR152 pKa = 11.84CC153 pKa = 4.07

Molecular weight: 17.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1370

130

494

274.0

30.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.635 ± 1.19

0.876 ± 0.475

4.453 ± 1.001

5.255 ± 0.986

4.307 ± 0.819

8.175 ± 0.633

2.044 ± 0.541

4.161 ± 0.252

4.745 ± 0.853

7.81 ± 0.389

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.044 ± 0.39

3.066 ± 0.49

7.007 ± 0.99

3.431 ± 0.62

7.883 ± 1.584

9.708 ± 0.979

4.307 ± 1.252

6.569 ± 0.966

1.241 ± 0.311

3.285 ± 0.735
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski