Rodent Torque teno virus 4
Average proteome isoelectric point is 8.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4QBT7|A0A2H4QBT7_9VIRU Capsid protein OS=Rodent Torque teno virus 4 OX=2054611 PE=3 SV=1
MM1 pKa = 7.49 LRR3 pKa = 11.84 RR4 pKa = 11.84 KK5 pKa = 7.3 TRR7 pKa = 11.84 HH8 pKa = 4.98 VPVMQWNPINRR19 pKa = 11.84 KK20 pKa = 8.52 KK21 pKa = 9.96 CTIRR25 pKa = 11.84 GFCPLLYY32 pKa = 10.61 CLGGQKK38 pKa = 10.87 GIMDD42 pKa = 4.02 FTYY45 pKa = 9.9 PANKK49 pKa = 10.17 AILNWMGGGVHH60 pKa = 6.64 SSLISLLDD68 pKa = 3.75 LFWEE72 pKa = 4.1 EE73 pKa = 4.41 RR74 pKa = 11.84 YY75 pKa = 8.89 WRR77 pKa = 11.84 ARR79 pKa = 11.84 WSSSNQGFNLFRR91 pKa = 11.84 YY92 pKa = 9.11 YY93 pKa = 11.13 GVTITLYY100 pKa = 8.28 PCMYY104 pKa = 7.98 YY105 pKa = 10.03 TYY107 pKa = 9.79 IFWYY111 pKa = 8.27 STEE114 pKa = 3.88 EE115 pKa = 3.83 LAEE118 pKa = 4.51 DD119 pKa = 4.07 SEE121 pKa = 4.75 PLTVCHH127 pKa = 6.82 PSQLLLSKK135 pKa = 10.23 HH136 pKa = 5.56 HH137 pKa = 6.36 VIVHH141 pKa = 6.44 RR142 pKa = 11.84 MHH144 pKa = 6.2 GMKK147 pKa = 10.19 VKK149 pKa = 9.95 PVKK152 pKa = 10.46 LRR154 pKa = 11.84 IKK156 pKa = 10.04 PPSQMTGTWHH166 pKa = 7.26 SFAKK170 pKa = 9.25 WAKK173 pKa = 9.75 QPLLKK178 pKa = 10.09 WRR180 pKa = 11.84 VSLCDD185 pKa = 4.57 IEE187 pKa = 5.3 NPWTGFPNSEE197 pKa = 3.98 TQGIHH202 pKa = 4.57 VKK204 pKa = 9.7 VWAISTTSTAAGEE217 pKa = 3.75 KK218 pKa = 10.13 DD219 pKa = 2.73 IWYY222 pKa = 9.35 FPLLDD227 pKa = 5.36 DD228 pKa = 5.03 GTDD231 pKa = 2.96 VWICLHH237 pKa = 5.8 EE238 pKa = 4.59 IKK240 pKa = 10.06 WNRR243 pKa = 11.84 NGDD246 pKa = 3.84 GPDD249 pKa = 3.32 EE250 pKa = 4.62 SSSNFWPVQAEE261 pKa = 4.2 FQKK264 pKa = 11.24 LLVPFYY270 pKa = 9.58 MYY272 pKa = 11.21 GFGRR276 pKa = 11.84 SATFYY281 pKa = 11.25 LMQKK285 pKa = 9.04 DD286 pKa = 3.86 VHH288 pKa = 6.22 MPAPSQGDD296 pKa = 3.01 KK297 pKa = 11.42 GKK299 pKa = 10.3 FLFVKK304 pKa = 9.18 TINSPAWRR312 pKa = 11.84 GADD315 pKa = 3.09 GGFPQFKK322 pKa = 10.52 DD323 pKa = 3.08 NQFYY327 pKa = 10.69 MKK329 pKa = 9.9 FSTVAQIAAQGPWCMKK345 pKa = 10.27 SIGNEE350 pKa = 3.84 THH352 pKa = 6.5 GVNLSMKK359 pKa = 10.25 YY360 pKa = 9.85 KK361 pKa = 10.62 FHH363 pKa = 6.2 FQWGGTPGTHH373 pKa = 7.08 LPPVQPADD381 pKa = 3.75 GGPPQPLLQSTIRR394 pKa = 11.84 WGNSLRR400 pKa = 11.84 ADD402 pKa = 3.61 LRR404 pKa = 11.84 DD405 pKa = 3.53 PTTVGDD411 pKa = 4.0 EE412 pKa = 4.19 VLHH415 pKa = 6.38 PEE417 pKa = 4.33 EE418 pKa = 5.55 LDD420 pKa = 3.39 SAGIINPRR428 pKa = 11.84 AFRR431 pKa = 11.84 RR432 pKa = 11.84 ITKK435 pKa = 9.2 PAVSTGSRR443 pKa = 11.84 SVGTLGCLRR452 pKa = 11.84 PAVWSAEE459 pKa = 3.74 KK460 pKa = 10.24 RR461 pKa = 11.84 QEE463 pKa = 3.99 PSSEE467 pKa = 3.54 EE468 pKa = 4.14 SAQEE472 pKa = 4.3 EE473 pKa = 4.87 YY474 pKa = 10.96 SSSEE478 pKa = 4.08 TGSEE482 pKa = 4.4 TEE484 pKa = 3.97 TEE486 pKa = 3.94 EE487 pKa = 3.71 MDD489 pKa = 3.44 RR490 pKa = 11.84 RR491 pKa = 11.84 RR492 pKa = 11.84 HH493 pKa = 4.36 QRR495 pKa = 11.84 RR496 pKa = 11.84 KK497 pKa = 9.91 RR498 pKa = 11.84 KK499 pKa = 9.71 RR500 pKa = 11.84 LEE502 pKa = 3.52 QLLRR506 pKa = 11.84 GLMTVGLKK514 pKa = 10.22 RR515 pKa = 11.84 PMMAGPSGSSQRR527 pKa = 11.84 SVSII531 pKa = 3.88
Molecular weight: 60.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.813
IPC2_protein 8.726
IPC_protein 8.624
Toseland 9.268
ProMoST 9.121
Dawson 9.589
Bjellqvist 9.399
Wikipedia 9.75
Rodwell 9.809
Grimsley 9.648
Solomon 9.619
Lehninger 9.575
Nozaki 9.516
DTASelect 9.311
Thurlkill 9.443
EMBOSS 9.736
Sillero 9.575
Patrickios 4.851
IPC_peptide 9.619
IPC2_peptide 8.126
IPC2.peptide.svr19 7.768
Protein with the highest isoelectric point:
>tr|A0A2H4QBT7|A0A2H4QBT7_9VIRU Capsid protein OS=Rodent Torque teno virus 4 OX=2054611 PE=3 SV=1
MM1 pKa = 7.49 LRR3 pKa = 11.84 RR4 pKa = 11.84 KK5 pKa = 7.3 TRR7 pKa = 11.84 HH8 pKa = 4.98 VPVMQWNPINRR19 pKa = 11.84 KK20 pKa = 8.52 KK21 pKa = 9.96 CTIRR25 pKa = 11.84 GFCPLLYY32 pKa = 10.61 CLGGQKK38 pKa = 10.87 GIMDD42 pKa = 4.02 FTYY45 pKa = 9.9 PANKK49 pKa = 10.17 AILNWMGGGVHH60 pKa = 6.64 SSLISLLDD68 pKa = 3.75 LFWEE72 pKa = 4.1 EE73 pKa = 4.41 RR74 pKa = 11.84 YY75 pKa = 8.89 WRR77 pKa = 11.84 ARR79 pKa = 11.84 WSSSNQGFNLFRR91 pKa = 11.84 YY92 pKa = 9.11 YY93 pKa = 11.13 GVTITLYY100 pKa = 8.28 PCMYY104 pKa = 7.98 YY105 pKa = 10.03 TYY107 pKa = 9.79 IFWYY111 pKa = 8.27 STEE114 pKa = 3.88 EE115 pKa = 3.83 LAEE118 pKa = 4.51 DD119 pKa = 4.07 SEE121 pKa = 4.75 PLTVCHH127 pKa = 6.82 PSQLLLSKK135 pKa = 10.23 HH136 pKa = 5.56 HH137 pKa = 6.36 VIVHH141 pKa = 6.44 RR142 pKa = 11.84 MHH144 pKa = 6.2 GMKK147 pKa = 10.19 VKK149 pKa = 9.95 PVKK152 pKa = 10.46 LRR154 pKa = 11.84 IKK156 pKa = 10.04 PPSQMTGTWHH166 pKa = 7.26 SFAKK170 pKa = 9.25 WAKK173 pKa = 9.75 QPLLKK178 pKa = 10.09 WRR180 pKa = 11.84 VSLCDD185 pKa = 4.57 IEE187 pKa = 5.3 NPWTGFPNSEE197 pKa = 3.98 TQGIHH202 pKa = 4.57 VKK204 pKa = 9.7 VWAISTTSTAAGEE217 pKa = 3.75 KK218 pKa = 10.13 DD219 pKa = 2.73 IWYY222 pKa = 9.35 FPLLDD227 pKa = 5.36 DD228 pKa = 5.03 GTDD231 pKa = 2.96 VWICLHH237 pKa = 5.8 EE238 pKa = 4.59 IKK240 pKa = 10.06 WNRR243 pKa = 11.84 NGDD246 pKa = 3.84 GPDD249 pKa = 3.32 EE250 pKa = 4.62 SSSNFWPVQAEE261 pKa = 4.2 FQKK264 pKa = 11.24 LLVPFYY270 pKa = 9.58 MYY272 pKa = 11.21 GFGRR276 pKa = 11.84 SATFYY281 pKa = 11.25 LMQKK285 pKa = 9.04 DD286 pKa = 3.86 VHH288 pKa = 6.22 MPAPSQGDD296 pKa = 3.01 KK297 pKa = 11.42 GKK299 pKa = 10.3 FLFVKK304 pKa = 9.18 TINSPAWRR312 pKa = 11.84 GADD315 pKa = 3.09 GGFPQFKK322 pKa = 10.52 DD323 pKa = 3.08 NQFYY327 pKa = 10.69 MKK329 pKa = 9.9 FSTVAQIAAQGPWCMKK345 pKa = 10.27 SIGNEE350 pKa = 3.84 THH352 pKa = 6.5 GVNLSMKK359 pKa = 10.25 YY360 pKa = 9.85 KK361 pKa = 10.62 FHH363 pKa = 6.2 FQWGGTPGTHH373 pKa = 7.08 LPPVQPADD381 pKa = 3.75 GGPPQPLLQSTIRR394 pKa = 11.84 WGNSLRR400 pKa = 11.84 ADD402 pKa = 3.61 LRR404 pKa = 11.84 DD405 pKa = 3.53 PTTVGDD411 pKa = 4.0 EE412 pKa = 4.19 VLHH415 pKa = 6.38 PEE417 pKa = 4.33 EE418 pKa = 5.55 LDD420 pKa = 3.39 SAGIINPRR428 pKa = 11.84 AFRR431 pKa = 11.84 RR432 pKa = 11.84 ITKK435 pKa = 9.2 PAVSTGSRR443 pKa = 11.84 SVGTLGCLRR452 pKa = 11.84 PAVWSAEE459 pKa = 3.74 KK460 pKa = 10.24 RR461 pKa = 11.84 QEE463 pKa = 3.99 PSSEE467 pKa = 3.54 EE468 pKa = 4.14 SAQEE472 pKa = 4.3 EE473 pKa = 4.87 YY474 pKa = 10.96 SSSEE478 pKa = 4.08 TGSEE482 pKa = 4.4 TEE484 pKa = 3.97 TEE486 pKa = 3.94 EE487 pKa = 3.71 MDD489 pKa = 3.44 RR490 pKa = 11.84 RR491 pKa = 11.84 RR492 pKa = 11.84 HH493 pKa = 4.36 QRR495 pKa = 11.84 RR496 pKa = 11.84 KK497 pKa = 9.91 RR498 pKa = 11.84 KK499 pKa = 9.71 RR500 pKa = 11.84 LEE502 pKa = 3.52 QLLRR506 pKa = 11.84 GLMTVGLKK514 pKa = 10.22 RR515 pKa = 11.84 PMMAGPSGSSQRR527 pKa = 11.84 SVSII531 pKa = 3.88
Molecular weight: 60.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.813
IPC2_protein 8.726
IPC_protein 8.624
Toseland 9.268
ProMoST 9.121
Dawson 9.589
Bjellqvist 9.399
Wikipedia 9.75
Rodwell 9.809
Grimsley 9.648
Solomon 9.619
Lehninger 9.575
Nozaki 9.516
DTASelect 9.311
Thurlkill 9.443
EMBOSS 9.736
Sillero 9.575
Patrickios 4.851
IPC_peptide 9.619
IPC2_peptide 8.126
IPC2.peptide.svr19 7.768
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
531
531
531
531.0
60.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.896 ± 0.0
1.695 ± 0.0
3.766 ± 0.0
5.273 ± 0.0
4.331 ± 0.0
8.098 ± 0.0
3.013 ± 0.0
4.331 ± 0.0
5.838 ± 0.0
7.91 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.39 ± 0.0
3.202 ± 0.0
6.968 ± 0.0
4.52 ± 0.0
6.403 ± 0.0
8.286 ± 0.0
6.026 ± 0.0
5.085 ± 0.0
3.766 ± 0.0
3.202 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here