Capybara microvirus Cap3_SP_475
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVU2|A0A4V1FVU2_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_475 OX=2585467 PE=4 SV=1
MM1 pKa = 7.81 LSTLCDD7 pKa = 3.96 FNKK10 pKa = 10.03 FDD12 pKa = 3.05 ISKK15 pKa = 8.32 TVRR18 pKa = 11.84 IKK20 pKa = 10.55 FSNIIITVFEE30 pKa = 4.22 SSNFDD35 pKa = 3.45 FDD37 pKa = 3.82 ITQEE41 pKa = 4.1 DD42 pKa = 4.37 KK43 pKa = 11.2 NLNDD47 pKa = 3.51 ISILNDD53 pKa = 3.81 SILEE57 pKa = 3.96 EE58 pKa = 4.31 LNKK61 pKa = 10.65 SFTNVKK67 pKa = 10.04 LL68 pKa = 3.61
Molecular weight: 7.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.662
IPC2_protein 4.507
IPC_protein 4.329
Toseland 4.139
ProMoST 4.482
Dawson 4.317
Bjellqvist 4.469
Wikipedia 4.24
Rodwell 4.164
Grimsley 4.05
Solomon 4.304
Lehninger 4.253
Nozaki 4.431
DTASelect 4.647
Thurlkill 4.19
EMBOSS 4.253
Sillero 4.457
Patrickios 4.342
IPC_peptide 4.304
IPC2_peptide 4.431
IPC2.peptide.svr19 4.409
Protein with the highest isoelectric point:
>tr|A0A4P8W503|A0A4P8W503_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_475 OX=2585467 PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.4 CFAPLRR8 pKa = 11.84 LKK10 pKa = 10.88 VRR12 pKa = 11.84 GATNLSGAPVFVPCGRR28 pKa = 11.84 CFACKK33 pKa = 9.83 QNNRR37 pKa = 11.84 NSWSFRR43 pKa = 11.84 LHH45 pKa = 6.49 NEE47 pKa = 3.56 ASSCLNSFFFTLTYY61 pKa = 9.63 NPKK64 pKa = 9.46 YY65 pKa = 9.66 VPKK68 pKa = 10.44 IKK70 pKa = 9.97 ICNSIFKK77 pKa = 9.08 TVSKK81 pKa = 10.7 RR82 pKa = 11.84 DD83 pKa = 3.25 VQLFIKK89 pKa = 10.46 RR90 pKa = 11.84 LRR92 pKa = 11.84 KK93 pKa = 9.71 NSGLKK98 pKa = 7.71 FTYY101 pKa = 10.39 YY102 pKa = 10.04 IISEE106 pKa = 4.19 YY107 pKa = 10.82 GPRR110 pKa = 11.84 TNRR113 pKa = 11.84 PHH115 pKa = 4.77 YY116 pKa = 10.15 HH117 pKa = 6.8 GIIFLYY123 pKa = 10.81 SKK125 pKa = 10.19 IDD127 pKa = 3.39 KK128 pKa = 10.86 DD129 pKa = 3.68 NLQKK133 pKa = 10.83 FISSSWFLGFCRR145 pKa = 11.84 VDD147 pKa = 3.41 SLSLARR153 pKa = 11.84 IEE155 pKa = 4.44 YY156 pKa = 7.69 VTAYY160 pKa = 8.77 TFKK163 pKa = 10.39 EE164 pKa = 3.98 CEE166 pKa = 4.0 PRR168 pKa = 11.84 FKK170 pKa = 10.63 KK171 pKa = 10.54 YY172 pKa = 9.95 QLPNFRR178 pKa = 11.84 LTSRR182 pKa = 11.84 RR183 pKa = 11.84 PALGSSFLEE192 pKa = 4.84 DD193 pKa = 3.66 YY194 pKa = 11.7 NNFDD198 pKa = 4.77 LIKK201 pKa = 10.51 AIEE204 pKa = 4.29 NGLTNIKK211 pKa = 10.84 YY212 pKa = 9.64 NDD214 pKa = 3.44 KK215 pKa = 10.63 FINIPRR221 pKa = 11.84 YY222 pKa = 8.9 YY223 pKa = 9.86 KK224 pKa = 10.84 NKK226 pKa = 9.79 IAPTSNSYY234 pKa = 11.24 EE235 pKa = 4.06 DD236 pKa = 4.8 LEE238 pKa = 4.78 NIYY241 pKa = 8.95 NTLSFQNDD249 pKa = 3.25 YY250 pKa = 11.02 NIKK253 pKa = 10.39 KK254 pKa = 9.51 FYY256 pKa = 10.3 KK257 pKa = 9.55 YY258 pKa = 11.29 YY259 pKa = 10.38 KK260 pKa = 9.61 IKK262 pKa = 10.26 YY263 pKa = 7.79 PNWDD267 pKa = 3.12 NKK269 pKa = 10.58 QILSYY274 pKa = 10.48 IFHH277 pKa = 7.01 NSNLTHH283 pKa = 5.91 NQLRR287 pKa = 11.84 FTIKK291 pKa = 10.44 QIFKK295 pKa = 10.29 NGKK298 pKa = 7.45 VQNLYY303 pKa = 10.72 DD304 pKa = 3.43
Molecular weight: 36.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.856
IPC2_protein 9.385
IPC_protein 9.297
Toseland 9.809
ProMoST 9.604
Dawson 10.087
Bjellqvist 9.809
Wikipedia 10.277
Rodwell 10.438
Grimsley 10.175
Solomon 10.101
Lehninger 10.058
Nozaki 9.882
DTASelect 9.78
Thurlkill 9.911
EMBOSS 10.248
Sillero 10.014
Patrickios 5.181
IPC_peptide 10.087
IPC2_peptide 8.58
IPC2.peptide.svr19 8.307
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1579
68
626
263.2
30.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.433 ± 0.858
1.267 ± 0.45
5.13 ± 0.532
4.687 ± 0.462
6.966 ± 0.537
3.737 ± 0.565
1.583 ± 0.29
7.346 ± 0.694
7.6 ± 1.35
8.93 ± 0.232
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.393 ± 0.27
9.88 ± 1.415
4.37 ± 0.756
5.13 ± 1.12
3.293 ± 0.747
8.233 ± 0.583
5.573 ± 0.732
4.053 ± 0.559
0.823 ± 0.163
5.573 ± 0.922
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here