Amino acid dipepetide frequency for Mycobacterium phage Yuna

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.971AlaAla: 20.971 ± 1.467
1.356AlaCys: 1.356 ± 0.273
8.086AlaAsp: 8.086 ± 0.797
9.808AlaGlu: 9.808 ± 0.824
2.974AlaPhe: 2.974 ± 0.5
8.66AlaGly: 8.66 ± 0.794
2.974AlaHis: 2.974 ± 0.392
5.165AlaIle: 5.165 ± 0.593
3.704AlaLys: 3.704 ± 0.465
11.633AlaLeu: 11.633 ± 0.786
3.86AlaMet: 3.86 ± 0.459
2.974AlaAsn: 2.974 ± 0.499
7.303AlaPro: 7.303 ± 0.679
4.956AlaGln: 4.956 ± 0.629
9.39AlaArg: 9.39 ± 0.733
4.747AlaSer: 4.747 ± 0.597
6.782AlaThr: 6.782 ± 0.704
11.112AlaVal: 11.112 ± 0.91
2.295AlaTrp: 2.295 ± 0.342
2.661AlaTyr: 2.661 ± 0.359
0.0AlaXaa: 0.0 ± 0.0
Cys
1.2CysAla: 1.2 ± 0.302
0.209CysCys: 0.209 ± 0.126
0.783CysAsp: 0.783 ± 0.184
0.835CysGlu: 0.835 ± 0.207
0.261CysPhe: 0.261 ± 0.128
1.356CysGly: 1.356 ± 0.318
0.261CysHis: 0.261 ± 0.133
0.313CysIle: 0.313 ± 0.127
0.365CysLys: 0.365 ± 0.151
0.939CysLeu: 0.939 ± 0.226
0.313CysMet: 0.313 ± 0.143
0.209CysAsn: 0.209 ± 0.108
0.835CysPro: 0.835 ± 0.209
0.209CysGln: 0.209 ± 0.109
0.939CysArg: 0.939 ± 0.229
1.043CysSer: 1.043 ± 0.262
0.574CysThr: 0.574 ± 0.203
0.47CysVal: 0.47 ± 0.148
0.313CysTrp: 0.313 ± 0.112
0.157CysTyr: 0.157 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
7.982AspAla: 7.982 ± 0.697
0.626AspCys: 0.626 ± 0.177
5.478AspAsp: 5.478 ± 0.717
5.843AspGlu: 5.843 ± 0.594
0.991AspPhe: 0.991 ± 0.208
6.156AspGly: 6.156 ± 0.632
1.304AspHis: 1.304 ± 0.261
1.2AspIle: 1.2 ± 0.276
1.356AspLys: 1.356 ± 0.295
6.364AspLeu: 6.364 ± 0.614
1.722AspMet: 1.722 ± 0.349
1.774AspAsn: 1.774 ± 0.285
3.808AspPro: 3.808 ± 0.517
2.087AspGln: 2.087 ± 0.337
4.382AspArg: 4.382 ± 0.527
3.078AspSer: 3.078 ± 0.466
3.182AspThr: 3.182 ± 0.395
5.008AspVal: 5.008 ± 0.46
0.991AspTrp: 0.991 ± 0.235
1.304AspTyr: 1.304 ± 0.24
0.0AspXaa: 0.0 ± 0.0
Glu
8.555GluAla: 8.555 ± 0.836
0.73GluCys: 0.73 ± 0.204
2.869GluAsp: 2.869 ± 0.383
0.887GluGlu: 0.887 ± 0.247
1.722GluPhe: 1.722 ± 0.329
5.321GluGly: 5.321 ± 0.584
1.722GluHis: 1.722 ± 0.326
1.982GluIle: 1.982 ± 0.294
1.93GluLys: 1.93 ± 0.357
5.06GluLeu: 5.06 ± 0.498
1.565GluMet: 1.565 ± 0.334
1.2GluAsn: 1.2 ± 0.193
3.652GluPro: 3.652 ± 0.519
2.765GluGln: 2.765 ± 0.365
4.33GluArg: 4.33 ± 0.472
2.452GluSer: 2.452 ± 0.307
2.661GluThr: 2.661 ± 0.37
5.478GluVal: 5.478 ± 0.634
1.2GluTrp: 1.2 ± 0.236
2.243GluTyr: 2.243 ± 0.404
0.0GluXaa: 0.0 ± 0.0
Phe
3.234PheAla: 3.234 ± 0.485
0.261PheCys: 0.261 ± 0.115
3.13PheAsp: 3.13 ± 0.45
1.2PheGlu: 1.2 ± 0.318
0.417PhePhe: 0.417 ± 0.151
2.921PheGly: 2.921 ± 0.463
0.209PheHis: 0.209 ± 0.113
0.939PheIle: 0.939 ± 0.178
1.043PheLys: 1.043 ± 0.263
1.669PheLeu: 1.669 ± 0.28
0.574PheMet: 0.574 ± 0.155
1.043PheAsn: 1.043 ± 0.291
1.304PhePro: 1.304 ± 0.232
1.043PheGln: 1.043 ± 0.293
1.669PheArg: 1.669 ± 0.289
0.887PheSer: 0.887 ± 0.263
1.669PheThr: 1.669 ± 0.289
2.191PheVal: 2.191 ± 0.344
0.417PheTrp: 0.417 ± 0.142
0.261PheTyr: 0.261 ± 0.141
0.0PheXaa: 0.0 ± 0.0
Gly
10.277GlyAla: 10.277 ± 0.901
1.148GlyCys: 1.148 ± 0.29
5.686GlyAsp: 5.686 ± 0.505
5.791GlyGlu: 5.791 ± 0.536
2.087GlyPhe: 2.087 ± 0.395
9.286GlyGly: 9.286 ± 1.272
1.826GlyHis: 1.826 ± 0.359
2.087GlyIle: 2.087 ± 0.378
4.017GlyLys: 4.017 ± 0.449
7.721GlyLeu: 7.721 ± 0.847
1.774GlyMet: 1.774 ± 0.258
3.391GlyAsn: 3.391 ± 0.5
3.182GlyPro: 3.182 ± 0.421
2.348GlyGln: 2.348 ± 0.379
5.999GlyArg: 5.999 ± 0.57
5.425GlySer: 5.425 ± 0.452
5.738GlyThr: 5.738 ± 0.709
7.408GlyVal: 7.408 ± 0.526
2.191GlyTrp: 2.191 ± 0.332
2.713GlyTyr: 2.713 ± 0.502
0.0GlyXaa: 0.0 ± 0.0
His
2.035HisAla: 2.035 ± 0.427
0.47HisCys: 0.47 ± 0.188
1.304HisAsp: 1.304 ± 0.323
0.939HisGlu: 0.939 ± 0.21
0.574HisPhe: 0.574 ± 0.168
2.556HisGly: 2.556 ± 0.358
0.417HisHis: 0.417 ± 0.137
0.574HisIle: 0.574 ± 0.162
0.47HisLys: 0.47 ± 0.16
2.035HisLeu: 2.035 ± 0.314
0.261HisMet: 0.261 ± 0.113
0.47HisAsn: 0.47 ± 0.151
1.043HisPro: 1.043 ± 0.254
0.73HisGln: 0.73 ± 0.207
2.191HisArg: 2.191 ± 0.405
0.783HisSer: 0.783 ± 0.188
1.252HisThr: 1.252 ± 0.241
2.191HisVal: 2.191 ± 0.295
0.522HisTrp: 0.522 ± 0.194
0.835HisTyr: 0.835 ± 0.195
0.0HisXaa: 0.0 ± 0.0
Ile
5.165IleAla: 5.165 ± 0.441
0.157IleCys: 0.157 ± 0.104
3.287IleAsp: 3.287 ± 0.379
3.495IleGlu: 3.495 ± 0.481
0.626IlePhe: 0.626 ± 0.18
4.121IleGly: 4.121 ± 0.756
0.417IleHis: 0.417 ± 0.171
0.365IleIle: 0.365 ± 0.144
0.939IleLys: 0.939 ± 0.175
2.921IleLeu: 2.921 ± 0.373
0.209IleMet: 0.209 ± 0.089
1.2IleAsn: 1.2 ± 0.282
1.409IlePro: 1.409 ± 0.282
0.939IleGln: 0.939 ± 0.247
2.348IleArg: 2.348 ± 0.316
1.356IleSer: 1.356 ± 0.229
1.774IleThr: 1.774 ± 0.256
3.287IleVal: 3.287 ± 0.383
0.574IleTrp: 0.574 ± 0.18
0.313IleTyr: 0.313 ± 0.092
0.0IleXaa: 0.0 ± 0.0
Lys
4.226LysAla: 4.226 ± 0.505
0.365LysCys: 0.365 ± 0.135
0.991LysAsp: 0.991 ± 0.236
0.47LysGlu: 0.47 ± 0.134
0.783LysPhe: 0.783 ± 0.242
2.921LysGly: 2.921 ± 0.429
0.73LysHis: 0.73 ± 0.197
1.409LysIle: 1.409 ± 0.298
0.417LysLys: 0.417 ± 0.153
3.287LysLeu: 3.287 ± 0.35
0.939LysMet: 0.939 ± 0.251
0.522LysAsn: 0.522 ± 0.183
2.452LysPro: 2.452 ± 0.364
0.887LysGln: 0.887 ± 0.236
2.556LysArg: 2.556 ± 0.411
1.096LysSer: 1.096 ± 0.196
1.878LysThr: 1.878 ± 0.331
2.817LysVal: 2.817 ± 0.349
0.678LysTrp: 0.678 ± 0.188
0.887LysTyr: 0.887 ± 0.257
0.0LysXaa: 0.0 ± 0.0
Leu
13.459LeuAla: 13.459 ± 0.787
0.522LeuCys: 0.522 ± 0.161
7.825LeuAsp: 7.825 ± 0.616
2.035LeuGlu: 2.035 ± 0.367
2.243LeuPhe: 2.243 ± 0.375
6.99LeuGly: 6.99 ± 0.562
2.452LeuHis: 2.452 ± 0.363
3.913LeuIle: 3.913 ± 0.471
2.295LeuLys: 2.295 ± 0.334
7.408LeuLeu: 7.408 ± 0.628
1.878LeuMet: 1.878 ± 0.368
2.139LeuAsn: 2.139 ± 0.39
4.434LeuPro: 4.434 ± 0.526
2.921LeuGln: 2.921 ± 0.375
6.782LeuArg: 6.782 ± 0.497
5.843LeuSer: 5.843 ± 0.453
4.747LeuThr: 4.747 ± 0.558
5.791LeuVal: 5.791 ± 0.574
1.565LeuTrp: 1.565 ± 0.32
1.774LeuTyr: 1.774 ± 0.36
0.0LeuXaa: 0.0 ± 0.0
Met
2.765MetAla: 2.765 ± 0.44
0.104MetCys: 0.104 ± 0.068
0.783MetAsp: 0.783 ± 0.167
0.574MetGlu: 0.574 ± 0.176
0.939MetPhe: 0.939 ± 0.239
1.565MetGly: 1.565 ± 0.279
0.626MetHis: 0.626 ± 0.175
0.73MetIle: 0.73 ± 0.18
0.261MetLys: 0.261 ± 0.122
1.774MetLeu: 1.774 ± 0.334
0.209MetMet: 0.209 ± 0.083
0.887MetAsn: 0.887 ± 0.212
1.304MetPro: 1.304 ± 0.312
1.2MetGln: 1.2 ± 0.223
1.565MetArg: 1.565 ± 0.261
2.452MetSer: 2.452 ± 0.335
2.139MetThr: 2.139 ± 0.345
1.774MetVal: 1.774 ± 0.291
0.417MetTrp: 0.417 ± 0.146
0.365MetTyr: 0.365 ± 0.128
0.0MetXaa: 0.0 ± 0.0
Asn
3.6AsnAla: 3.6 ± 0.47
0.365AsnCys: 0.365 ± 0.136
1.096AsnAsp: 1.096 ± 0.258
1.252AsnGlu: 1.252 ± 0.251
0.783AsnPhe: 0.783 ± 0.22
3.547AsnGly: 3.547 ± 0.558
0.365AsnHis: 0.365 ± 0.118
0.783AsnIle: 0.783 ± 0.254
0.678AsnLys: 0.678 ± 0.207
2.139AsnLeu: 2.139 ± 0.342
0.47AsnMet: 0.47 ± 0.137
0.783AsnAsn: 0.783 ± 0.286
2.243AsnPro: 2.243 ± 0.379
0.574AsnGln: 0.574 ± 0.158
2.191AsnArg: 2.191 ± 0.328
0.626AsnSer: 0.626 ± 0.17
2.139AsnThr: 2.139 ± 0.333
2.243AsnVal: 2.243 ± 0.275
0.522AsnTrp: 0.522 ± 0.136
0.574AsnTyr: 0.574 ± 0.146
0.0AsnXaa: 0.0 ± 0.0
Pro
8.295ProAla: 8.295 ± 0.633
0.47ProCys: 0.47 ± 0.188
3.026ProAsp: 3.026 ± 0.369
4.486ProGlu: 4.486 ± 0.507
1.878ProPhe: 1.878 ± 0.35
5.425ProGly: 5.425 ± 0.538
0.939ProHis: 0.939 ± 0.208
1.617ProIle: 1.617 ± 0.267
1.617ProLys: 1.617 ± 0.317
4.591ProLeu: 4.591 ± 0.49
0.991ProMet: 0.991 ± 0.28
1.096ProAsn: 1.096 ± 0.295
2.974ProPro: 2.974 ± 0.437
1.2ProGln: 1.2 ± 0.276
3.13ProArg: 3.13 ± 0.366
2.817ProSer: 2.817 ± 0.439
3.287ProThr: 3.287 ± 0.319
4.539ProVal: 4.539 ± 0.605
0.887ProTrp: 0.887 ± 0.153
1.461ProTyr: 1.461 ± 0.292
0.0ProXaa: 0.0 ± 0.0
Gln
4.486GlnAla: 4.486 ± 0.472
0.313GlnCys: 0.313 ± 0.124
1.461GlnAsp: 1.461 ± 0.342
0.783GlnGlu: 0.783 ± 0.171
1.2GlnPhe: 1.2 ± 0.259
2.556GlnGly: 2.556 ± 0.273
0.887GlnHis: 0.887 ± 0.161
2.556GlnIle: 2.556 ± 0.361
0.887GlnLys: 0.887 ± 0.201
2.243GlnLeu: 2.243 ± 0.333
0.783GlnMet: 0.783 ± 0.23
0.939GlnAsn: 0.939 ± 0.25
2.556GlnPro: 2.556 ± 0.349
1.722GlnGln: 1.722 ± 0.267
3.13GlnArg: 3.13 ± 0.369
1.252GlnSer: 1.252 ± 0.236
1.878GlnThr: 1.878 ± 0.32
2.921GlnVal: 2.921 ± 0.398
0.887GlnTrp: 0.887 ± 0.262
0.73GlnTyr: 0.73 ± 0.209
0.0GlnXaa: 0.0 ± 0.0
Arg
6.886ArgAla: 6.886 ± 0.607
1.096ArgCys: 1.096 ± 0.261
4.591ArgAsp: 4.591 ± 0.477
4.539ArgGlu: 4.539 ± 0.523
2.035ArgPhe: 2.035 ± 0.295
5.373ArgGly: 5.373 ± 0.548
1.669ArgHis: 1.669 ± 0.28
3.078ArgIle: 3.078 ± 0.41
3.391ArgLys: 3.391 ± 0.426
6.677ArgLeu: 6.677 ± 0.568
2.452ArgMet: 2.452 ± 0.352
2.348ArgAsn: 2.348 ± 0.339
3.339ArgPro: 3.339 ± 0.439
2.661ArgGln: 2.661 ± 0.373
6.521ArgArg: 6.521 ± 0.756
3.808ArgSer: 3.808 ± 0.444
4.226ArgThr: 4.226 ± 0.594
5.112ArgVal: 5.112 ± 0.533
2.035ArgTrp: 2.035 ± 0.375
1.722ArgTyr: 1.722 ± 0.355
0.0ArgXaa: 0.0 ± 0.0
Ser
6.782SerAla: 6.782 ± 0.787
0.313SerCys: 0.313 ± 0.133
2.817SerAsp: 2.817 ± 0.325
3.287SerGlu: 3.287 ± 0.399
1.617SerPhe: 1.617 ± 0.287
4.695SerGly: 4.695 ± 0.602
1.148SerHis: 1.148 ± 0.262
1.669SerIle: 1.669 ± 0.259
1.617SerLys: 1.617 ± 0.276
4.747SerLeu: 4.747 ± 0.44
1.252SerMet: 1.252 ± 0.264
1.2SerAsn: 1.2 ± 0.223
2.765SerPro: 2.765 ± 0.358
1.565SerGln: 1.565 ± 0.299
3.287SerArg: 3.287 ± 0.367
3.026SerSer: 3.026 ± 0.47
2.556SerThr: 2.556 ± 0.328
3.339SerVal: 3.339 ± 0.363
1.148SerTrp: 1.148 ± 0.212
1.252SerTyr: 1.252 ± 0.234
0.0SerXaa: 0.0 ± 0.0
Thr
6.99ThrAla: 6.99 ± 0.66
0.783ThrCys: 0.783 ± 0.187
3.078ThrAsp: 3.078 ± 0.478
3.913ThrGlu: 3.913 ± 0.395
1.826ThrPhe: 1.826 ± 0.304
5.634ThrGly: 5.634 ± 0.626
1.304ThrHis: 1.304 ± 0.301
2.661ThrIle: 2.661 ± 0.391
2.139ThrLys: 2.139 ± 0.341
4.069ThrLeu: 4.069 ± 0.442
0.939ThrMet: 0.939 ± 0.171
1.096ThrAsn: 1.096 ± 0.224
3.391ThrPro: 3.391 ± 0.504
1.826ThrGln: 1.826 ± 0.328
3.756ThrArg: 3.756 ± 0.474
2.608ThrSer: 2.608 ± 0.433
3.182ThrThr: 3.182 ± 0.346
4.904ThrVal: 4.904 ± 0.536
1.409ThrTrp: 1.409 ± 0.194
1.304ThrTyr: 1.304 ± 0.223
0.0ThrXaa: 0.0 ± 0.0
Val
9.651ValAla: 9.651 ± 0.862
1.513ValCys: 1.513 ± 0.282
5.373ValAsp: 5.373 ± 0.463
6.417ValGlu: 6.417 ± 0.921
2.191ValPhe: 2.191 ± 0.415
7.095ValGly: 7.095 ± 0.737
1.617ValHis: 1.617 ± 0.333
2.452ValIle: 2.452 ± 0.371
2.452ValLys: 2.452 ± 0.42
6.782ValLeu: 6.782 ± 0.53
1.409ValMet: 1.409 ± 0.287
2.243ValAsn: 2.243 ± 0.377
4.852ValPro: 4.852 ± 0.504
2.191ValGln: 2.191 ± 0.344
5.373ValArg: 5.373 ± 0.535
4.382ValSer: 4.382 ± 0.514
4.278ValThr: 4.278 ± 0.478
6.521ValVal: 6.521 ± 0.758
2.452ValTrp: 2.452 ± 0.43
1.461ValTyr: 1.461 ± 0.227
0.0ValXaa: 0.0 ± 0.0
Trp
2.504TrpAla: 2.504 ± 0.35
0.417TrpCys: 0.417 ± 0.162
1.252TrpAsp: 1.252 ± 0.249
0.626TrpGlu: 0.626 ± 0.174
0.365TrpPhe: 0.365 ± 0.156
1.565TrpGly: 1.565 ± 0.305
0.626TrpHis: 0.626 ± 0.206
0.835TrpIle: 0.835 ± 0.187
0.261TrpLys: 0.261 ± 0.118
2.765TrpLeu: 2.765 ± 0.449
0.365TrpMet: 0.365 ± 0.144
0.574TrpAsn: 0.574 ± 0.136
0.991TrpPro: 0.991 ± 0.192
1.304TrpGln: 1.304 ± 0.25
2.139TrpArg: 2.139 ± 0.34
1.2TrpSer: 1.2 ± 0.229
1.252TrpThr: 1.252 ± 0.227
1.513TrpVal: 1.513 ± 0.278
0.313TrpTrp: 0.313 ± 0.137
0.574TrpTyr: 0.574 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.348TyrAla: 2.348 ± 0.315
0.365TyrCys: 0.365 ± 0.128
1.722TyrAsp: 1.722 ± 0.321
1.148TyrGlu: 1.148 ± 0.23
0.574TyrPhe: 0.574 ± 0.171
2.348TyrGly: 2.348 ± 0.351
0.157TyrHis: 0.157 ± 0.087
0.574TyrIle: 0.574 ± 0.162
0.47TyrLys: 0.47 ± 0.167
2.191TyrLeu: 2.191 ± 0.398
0.417TyrMet: 0.417 ± 0.155
0.887TyrAsn: 0.887 ± 0.192
0.939TyrPro: 0.939 ± 0.222
1.043TyrGln: 1.043 ± 0.197
1.878TyrArg: 1.878 ± 0.348
1.2TyrSer: 1.2 ± 0.237
1.617TyrThr: 1.617 ± 0.322
2.087TyrVal: 2.087 ± 0.289
0.626TyrTrp: 0.626 ± 0.239
0.522TyrTyr: 0.522 ± 0.156
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 99 proteins (19170 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski