Amino acid dipepetide frequency for Pseudothermotoga thermarum DSM 5069

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.324AlaAla: 5.324 ± 0.125	0.722AlaCys: 0.722 ± 0.036	2.796AlaAsp: 2.796 ± 0.074	4.253AlaGlu: 4.253 ± 0.095	3.381AlaPhe: 3.381 ± 0.074	4.599AlaGly: 4.599 ± 0.09	0.961AlaHis: 0.961 ± 0.043	5.605AlaIle: 5.605 ± 0.115	5.581AlaLys: 5.581 ± 0.093	7.615AlaLeu: 7.615 ± 0.13	1.817AlaMet: 1.817 ± 0.067	2.362AlaAsn: 2.362 ± 0.063	1.782AlaPro: 1.782 ± 0.055	1.957AlaGln: 1.957 ± 0.067	3.268AlaArg: 3.268 ± 0.072	3.692AlaSer: 3.692 ± 0.084	3.152AlaThr: 3.152 ± 0.079	6.038AlaVal: 6.038 ± 0.114	0.601AlaTrp: 0.601 ± 0.033	2.341AlaTyr: 2.341 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	0.61CysAla: 0.61 ± 0.036	0.122CysCys: 0.122 ± 0.016	0.522CysAsp: 0.522 ± 0.033	0.771CysGlu: 0.771 ± 0.036	0.47CysPhe: 0.47 ± 0.028	0.987CysGly: 0.987 ± 0.04	0.215CysHis: 0.215 ± 0.02	0.543CysIle: 0.543 ± 0.029	0.667CysLys: 0.667 ± 0.032	0.892CysLeu: 0.892 ± 0.037	0.206CysMet: 0.206 ± 0.02	0.311CysAsn: 0.311 ± 0.024	0.525CysPro: 0.525 ± 0.03	0.293CysGln: 0.293 ± 0.021	0.324CysArg: 0.324 ± 0.024	0.592CysSer: 0.592 ± 0.035	0.372CysThr: 0.372 ± 0.024	0.801CysVal: 0.801 ± 0.04	0.114CysTrp: 0.114 ± 0.014	0.319CysTyr: 0.319 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	2.63AspAla: 2.63 ± 0.062	0.475AspCys: 0.475 ± 0.031	1.839AspAsp: 1.839 ± 0.058	4.422AspGlu: 4.422 ± 0.093	3.019AspPhe: 3.019 ± 0.078	3.193AspGly: 3.193 ± 0.082	0.715AspHis: 0.715 ± 0.036	3.502AspIle: 3.502 ± 0.074	3.165AspLys: 3.165 ± 0.084	5.508AspLeu: 5.508 ± 0.105	0.915AspMet: 0.915 ± 0.04	1.263AspAsn: 1.263 ± 0.047	2.616AspPro: 2.616 ± 0.075	1.372AspGln: 1.372 ± 0.047	1.936AspArg: 1.936 ± 0.062	2.146AspSer: 2.146 ± 0.063	1.678AspThr: 1.678 ± 0.059	4.337AspVal: 4.337 ± 0.084	0.584AspTrp: 0.584 ± 0.031	1.965AspTyr: 1.965 ± 0.058	0.0AspXaa: 0.0 ± 0.0
Glu	4.653GluAla: 4.653 ± 0.097	0.468GluCys: 0.468 ± 0.026	3.396GluAsp: 3.396 ± 0.079	6.693GluGlu: 6.693 ± 0.137	3.162GluPhe: 3.162 ± 0.073	4.058GluGly: 4.058 ± 0.077	0.954GluHis: 0.954 ± 0.036	6.786GluIle: 6.786 ± 0.124	8.453GluLys: 8.453 ± 0.129	7.138GluLeu: 7.138 ± 0.128	1.878GluMet: 1.878 ± 0.055	3.785GluAsn: 3.785 ± 0.101	2.195GluPro: 2.195 ± 0.062	2.281GluGln: 2.281 ± 0.071	3.887GluArg: 3.887 ± 0.087	3.203GluSer: 3.203 ± 0.067	3.282GluThr: 3.282 ± 0.082	5.192GluVal: 5.192 ± 0.102	0.606GluTrp: 0.606 ± 0.033	2.625GluTyr: 2.625 ± 0.069	0.0GluXaa: 0.0 ± 0.0
Phe	3.794PheAla: 3.794 ± 0.079	0.59PheCys: 0.59 ± 0.038	2.972PheAsp: 2.972 ± 0.074	4.31PheGlu: 4.31 ± 0.078	2.729PhePhe: 2.729 ± 0.075	3.911PheGly: 3.911 ± 0.084	0.792PheHis: 0.792 ± 0.038	3.04PheIle: 3.04 ± 0.083	3.338PheLys: 3.338 ± 0.075	5.626PheLeu: 5.626 ± 0.106	1.04PheMet: 1.04 ± 0.041	1.762PheAsn: 1.762 ± 0.051	1.801PhePro: 1.801 ± 0.057	1.396PheGln: 1.396 ± 0.049	1.707PheArg: 1.707 ± 0.048	3.593PheSer: 3.593 ± 0.084	2.198PheThr: 2.198 ± 0.065	4.71PheVal: 4.71 ± 0.09	0.523PheTrp: 0.523 ± 0.03	1.765PheTyr: 1.765 ± 0.064	0.0PheXaa: 0.0 ± 0.0
Gly	4.274GlyAla: 4.274 ± 0.117	0.777GlyCys: 0.777 ± 0.037	2.608GlyAsp: 2.608 ± 0.069	4.147GlyGlu: 4.147 ± 0.086	3.705GlyPhe: 3.705 ± 0.073	4.259GlyGly: 4.259 ± 0.099	1.052GlyHis: 1.052 ± 0.043	5.865GlyIle: 5.865 ± 0.092	5.807GlyLys: 5.807 ± 0.093	6.29GlyLeu: 6.29 ± 0.127	1.827GlyMet: 1.827 ± 0.055	2.222GlyAsn: 2.222 ± 0.057	1.912GlyPro: 1.912 ± 0.061	1.808GlyGln: 1.808 ± 0.06	2.84GlyArg: 2.84 ± 0.07	3.458GlySer: 3.458 ± 0.075	3.575GlyThr: 3.575 ± 0.088	5.131GlyVal: 5.131 ± 0.096	0.801GlyTrp: 0.801 ± 0.038	2.929GlyTyr: 2.929 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	0.971HisAla: 0.971 ± 0.044	0.14HisCys: 0.14 ± 0.016	0.657HisAsp: 0.657 ± 0.034	0.998HisGlu: 0.998 ± 0.04	0.78HisPhe: 0.78 ± 0.037	1.205HisGly: 1.205 ± 0.047	0.324HisHis: 0.324 ± 0.025	1.06HisIle: 1.06 ± 0.042	0.769HisLys: 0.769 ± 0.033	1.582HisLeu: 1.582 ± 0.051	0.371HisMet: 0.371 ± 0.022	0.523HisAsn: 0.523 ± 0.028	0.946HisPro: 0.946 ± 0.042	0.441HisGln: 0.441 ± 0.027	0.709HisArg: 0.709 ± 0.027	0.834HisSer: 0.834 ± 0.037	0.71HisThr: 0.71 ± 0.035	1.214HisVal: 1.214 ± 0.042	0.128HisTrp: 0.128 ± 0.013	0.517HisTyr: 0.517 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.994IleAla: 5.994 ± 0.105	0.853IleCys: 0.853 ± 0.04	4.404IleAsp: 4.404 ± 0.085	5.852IleGlu: 5.852 ± 0.111	4.428IlePhe: 4.428 ± 0.1	5.178IleGly: 5.178 ± 0.11	1.133IleHis: 1.133 ± 0.035	4.984IleIle: 4.984 ± 0.113	5.14IleLys: 5.14 ± 0.099	8.551IleLeu: 8.551 ± 0.122	1.551IleMet: 1.551 ± 0.053	2.728IleAsn: 2.728 ± 0.063	3.51IlePro: 3.51 ± 0.081	1.835IleGln: 1.835 ± 0.055	3.207IleArg: 3.207 ± 0.073	5.152IleSer: 5.152 ± 0.106	3.731IleThr: 3.731 ± 0.079	7.044IleVal: 7.044 ± 0.107	0.735IleTrp: 0.735 ± 0.032	2.616IleTyr: 2.616 ± 0.065	0.0IleXaa: 0.0 ± 0.0
Lys	5.404LysAla: 5.404 ± 0.087	0.567LysCys: 0.567 ± 0.035	4.23LysAsp: 4.23 ± 0.095	6.329LysGlu: 6.329 ± 0.122	3.136LysPhe: 3.136 ± 0.072	4.375LysGly: 4.375 ± 0.081	1.084LysHis: 1.084 ± 0.037	7.255LysIle: 7.255 ± 0.134	6.126LysLys: 6.126 ± 0.106	8.06LysLeu: 8.06 ± 0.116	2.016LysMet: 2.016 ± 0.053	3.586LysAsn: 3.586 ± 0.072	3.032LysPro: 3.032 ± 0.075	2.437LysGln: 2.437 ± 0.07	3.671LysArg: 3.671 ± 0.083	4.006LysSer: 4.006 ± 0.076	3.806LysThr: 3.806 ± 0.075	7.023LysVal: 7.023 ± 0.111	0.717LysTrp: 0.717 ± 0.04	2.838LysTyr: 2.838 ± 0.072	0.0LysXaa: 0.0 ± 0.0
Leu	7.572LeuAla: 7.572 ± 0.118	1.011LeuCys: 1.011 ± 0.046	4.924LeuAsp: 4.924 ± 0.104	7.777LeuGlu: 7.777 ± 0.121	4.814LeuPhe: 4.814 ± 0.104	6.612LeuGly: 6.612 ± 0.104	1.288LeuHis: 1.288 ± 0.047	7.527LeuIle: 7.527 ± 0.144	9.009LeuLys: 9.009 ± 0.121	10.362LeuLeu: 10.362 ± 0.177	2.429LeuMet: 2.429 ± 0.057	4.092LeuAsn: 4.092 ± 0.077	4.061LeuPro: 4.061 ± 0.073	3.129LeuGln: 3.129 ± 0.071	4.848LeuArg: 4.848 ± 0.093	7.19LeuSer: 7.19 ± 0.129	5.119LeuThr: 5.119 ± 0.095	7.828LeuVal: 7.828 ± 0.117	0.949LeuTrp: 0.949 ± 0.04	3.172LeuTyr: 3.172 ± 0.07	0.0LeuXaa: 0.0 ± 0.0
Met	1.644MetAla: 1.644 ± 0.058	0.19MetCys: 0.19 ± 0.016	1.049MetAsp: 1.049 ± 0.038	1.583MetGlu: 1.583 ± 0.056	0.938MetPhe: 0.938 ± 0.038	1.45MetGly: 1.45 ± 0.055	0.275MetHis: 0.275 ± 0.02	2.084MetIle: 2.084 ± 0.055	2.491MetLys: 2.491 ± 0.06	2.24MetLeu: 2.24 ± 0.065	0.613MetMet: 0.613 ± 0.035	1.162MetAsn: 1.162 ± 0.044	0.964MetPro: 0.964 ± 0.037	0.593MetGln: 0.593 ± 0.035	1.403MetArg: 1.403 ± 0.051	1.24MetSer: 1.24 ± 0.041	1.076MetThr: 1.076 ± 0.04	1.892MetVal: 1.892 ± 0.055	0.174MetTrp: 0.174 ± 0.017	0.784MetTyr: 0.784 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	2.627AsnAla: 2.627 ± 0.067	0.496AsnCys: 0.496 ± 0.031	1.626AsnAsp: 1.626 ± 0.047	2.447AsnGlu: 2.447 ± 0.065	2.354AsnPhe: 2.354 ± 0.072	2.601AsnGly: 2.601 ± 0.073	0.595AsnHis: 0.595 ± 0.029	2.822AsnIle: 2.822 ± 0.071	2.279AsnLys: 2.279 ± 0.054	4.949AsnLeu: 4.949 ± 0.094	0.756AsnMet: 0.756 ± 0.034	1.353AsnAsn: 1.353 ± 0.051	2.117AsnPro: 2.117 ± 0.053	1.151AsnGln: 1.151 ± 0.038	1.478AsnArg: 1.478 ± 0.046	1.951AsnSer: 1.951 ± 0.061	1.458AsnThr: 1.458 ± 0.051	3.692AsnVal: 3.692 ± 0.08	0.465AsnTrp: 0.465 ± 0.028	1.561AsnTyr: 1.561 ± 0.054	0.0AsnXaa: 0.0 ± 0.0
Pro	2.286ProAla: 2.286 ± 0.065	0.309ProCys: 0.309 ± 0.024	2.12ProAsp: 2.12 ± 0.06	2.925ProGlu: 2.925 ± 0.07	2.053ProPhe: 2.053 ± 0.055	2.53ProGly: 2.53 ± 0.063	0.676ProHis: 0.676 ± 0.038	2.903ProIle: 2.903 ± 0.055	2.777ProLys: 2.777 ± 0.073	3.411ProLeu: 3.411 ± 0.072	0.871ProMet: 0.871 ± 0.031	1.678ProAsn: 1.678 ± 0.054	1.268ProPro: 1.268 ± 0.043	1.123ProGln: 1.123 ± 0.042	1.426ProArg: 1.426 ± 0.045	2.308ProSer: 2.308 ± 0.058	2.165ProThr: 2.165 ± 0.057	3.214ProVal: 3.214 ± 0.074	0.454ProTrp: 0.454 ± 0.029	1.465ProTyr: 1.465 ± 0.051	0.0ProXaa: 0.0 ± 0.0
Gln	2.047GlnAla: 2.047 ± 0.056	0.158GlnCys: 0.158 ± 0.014	1.211GlnAsp: 1.211 ± 0.045	2.188GlnGlu: 2.188 ± 0.063	1.164GlnPhe: 1.164 ± 0.039	1.435GlnGly: 1.435 ± 0.046	0.424GlnHis: 0.424 ± 0.03	2.926GlnIle: 2.926 ± 0.066	2.598GlnLys: 2.598 ± 0.064	3.115GlnLeu: 3.115 ± 0.063	0.907GlnMet: 0.907 ± 0.036	1.357GlnAsn: 1.357 ± 0.046	1.037GlnPro: 1.037 ± 0.04	1.151GlnGln: 1.151 ± 0.052	1.551GlnArg: 1.551 ± 0.06	1.338GlnSer: 1.338 ± 0.053	1.383GlnThr: 1.383 ± 0.044	2.213GlnVal: 2.213 ± 0.062	0.229GlnTrp: 0.229 ± 0.018	0.853GlnTyr: 0.853 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.478ArgAla: 2.478 ± 0.065	0.457ArgCys: 0.457 ± 0.032	1.726ArgAsp: 1.726 ± 0.055	3.31ArgGlu: 3.31 ± 0.077	2.424ArgPhe: 2.424 ± 0.066	2.437ArgGly: 2.437 ± 0.066	0.644ArgHis: 0.644 ± 0.032	4.342ArgIle: 4.342 ± 0.09	4.178ArgLys: 4.178 ± 0.083	4.352ArgLeu: 4.352 ± 0.092	1.434ArgMet: 1.434 ± 0.052	1.938ArgAsn: 1.938 ± 0.052	1.362ArgPro: 1.362 ± 0.05	1.418ArgGln: 1.418 ± 0.044	2.399ArgArg: 2.399 ± 0.068	2.139ArgSer: 2.139 ± 0.063	2.022ArgThr: 2.022 ± 0.064	2.902ArgVal: 2.902 ± 0.072	0.457ArgTrp: 0.457 ± 0.023	1.985ArgTyr: 1.985 ± 0.063	0.0ArgXaa: 0.0 ± 0.0
Ser	3.503SerAla: 3.503 ± 0.09	0.58SerCys: 0.58 ± 0.035	2.458SerAsp: 2.458 ± 0.074	3.606SerGlu: 3.606 ± 0.082	3.394SerPhe: 3.394 ± 0.101	3.947SerGly: 3.947 ± 0.082	1.0SerHis: 1.0 ± 0.038	4.422SerIle: 4.422 ± 0.087	4.576SerLys: 4.576 ± 0.081	5.932SerLeu: 5.932 ± 0.117	1.424SerMet: 1.424 ± 0.049	2.177SerAsn: 2.177 ± 0.064	2.058SerPro: 2.058 ± 0.069	1.91SerGln: 1.91 ± 0.056	2.304SerArg: 2.304 ± 0.061	3.52SerSer: 3.52 ± 0.091	2.712SerThr: 2.712 ± 0.067	4.119SerVal: 4.119 ± 0.074	0.641SerTrp: 0.641 ± 0.034	2.029SerTyr: 2.029 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	3.22ThrAla: 3.22 ± 0.069	0.432ThrCys: 0.432 ± 0.024	2.014ThrAsp: 2.014 ± 0.058	2.38ThrGlu: 2.38 ± 0.07	2.586ThrPhe: 2.586 ± 0.066	3.664ThrGly: 3.664 ± 0.075	0.801ThrHis: 0.801 ± 0.035	3.941ThrIle: 3.941 ± 0.083	3.17ThrLys: 3.17 ± 0.063	4.862ThrLeu: 4.862 ± 0.093	1.086ThrMet: 1.086 ± 0.037	1.788ThrAsn: 1.788 ± 0.051	2.027ThrPro: 2.027 ± 0.06	1.179ThrGln: 1.179 ± 0.042	1.894ThrArg: 1.894 ± 0.052	2.669ThrSer: 2.669 ± 0.069	2.621ThrThr: 2.621 ± 0.076	4.261ThrVal: 4.261 ± 0.089	0.468ThrTrp: 0.468 ± 0.026	1.647ThrTyr: 1.647 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	5.864ValAla: 5.864 ± 0.116	0.891ValCys: 0.891 ± 0.045	4.339ValAsp: 4.339 ± 0.085	7.286ValGlu: 7.286 ± 0.12	4.446ValPhe: 4.446 ± 0.101	5.576ValGly: 5.576 ± 0.108	1.127ValHis: 1.127 ± 0.044	5.883ValIle: 5.883 ± 0.095	6.43ValLys: 6.43 ± 0.104	8.288ValLeu: 8.288 ± 0.111	1.746ValMet: 1.746 ± 0.054	2.861ValAsn: 2.861 ± 0.069	2.938ValPro: 2.938 ± 0.072	2.378ValGln: 2.378 ± 0.065	3.403ValArg: 3.403 ± 0.076	4.628ValSer: 4.628 ± 0.089	3.581ValThr: 3.581 ± 0.077	7.896ValVal: 7.896 ± 0.116	0.689ValTrp: 0.689 ± 0.038	2.993ValTyr: 2.993 ± 0.073	0.0ValXaa: 0.0 ± 0.0
Trp	0.618TrpAla: 0.618 ± 0.033	0.088TrpCys: 0.088 ± 0.012	0.463TrpAsp: 0.463 ± 0.029	0.681TrpGlu: 0.681 ± 0.035	0.59TrpPhe: 0.59 ± 0.028	0.6TrpGly: 0.6 ± 0.036	0.171TrpHis: 0.171 ± 0.018	0.842TrpIle: 0.842 ± 0.04	0.738TrpLys: 0.738 ± 0.038	1.055TrpLeu: 1.055 ± 0.037	0.32TrpMet: 0.32 ± 0.022	0.486TrpAsn: 0.486 ± 0.031	0.255TrpPro: 0.255 ± 0.02	0.371TrpGln: 0.371 ± 0.025	0.489TrpArg: 0.489 ± 0.031	0.506TrpSer: 0.506 ± 0.026	0.418TrpThr: 0.418 ± 0.029	0.658TrpVal: 0.658 ± 0.034	0.208TrpTrp: 0.208 ± 0.02	0.413TrpTyr: 0.413 ± 0.028	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.338TyrAla: 2.338 ± 0.064	0.327TyrCys: 0.327 ± 0.024	1.926TyrAsp: 1.926 ± 0.058	2.738TyrGlu: 2.738 ± 0.071	2.024TyrPhe: 2.024 ± 0.067	2.671TyrGly: 2.671 ± 0.065	0.649TyrHis: 0.649 ± 0.032	2.294TyrIle: 2.294 ± 0.059	2.406TyrLys: 2.406 ± 0.068	3.885TyrLeu: 3.885 ± 0.076	0.636TyrMet: 0.636 ± 0.035	1.341TyrAsn: 1.341 ± 0.045	1.556TyrPro: 1.556 ± 0.05	1.063TyrGln: 1.063 ± 0.042	1.661TyrArg: 1.661 ± 0.051	2.148TyrSer: 2.148 ± 0.053	1.652TyrThr: 1.652 ± 0.051	3.077TyrVal: 3.077 ± 0.069	0.426TyrTrp: 0.426 ± 0.028	1.4TyrTyr: 1.4 ± 0.058	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1928 proteins (615132 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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