Borna disease virus (strain V) (BDV)
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P0C799-2|PHOSP-2_BDVV Isoform of P0C799 Isoform p16 of Phosphoprotein OS=Borna disease virus (strain V) OX=928296 GN=P/X PE=1 SV=1
MM1 pKa = 7.76 ISDD4 pKa = 4.12 PDD6 pKa = 3.4 QRR8 pKa = 11.84 TGRR11 pKa = 11.84 EE12 pKa = 3.69 QLSNDD17 pKa = 3.48 EE18 pKa = 4.78 LIKK21 pKa = 11.06 KK22 pKa = 9.74 LVTEE26 pKa = 4.26 LAEE29 pKa = 3.91 NSMIEE34 pKa = 4.03 AEE36 pKa = 4.42 EE37 pKa = 4.07 VRR39 pKa = 11.84 GTLGDD44 pKa = 3.36 ISARR48 pKa = 11.84 IEE50 pKa = 3.93 AGFEE54 pKa = 4.21 SLSALQVEE62 pKa = 5.61 TIQTAQRR69 pKa = 11.84 CDD71 pKa = 3.27 HH72 pKa = 6.83 SDD74 pKa = 3.55 SIRR77 pKa = 11.84 ILGEE81 pKa = 3.61 NIKK84 pKa = 10.55 ILDD87 pKa = 3.75 RR88 pKa = 11.84 SMKK91 pKa = 10.56 TMMEE95 pKa = 4.08 TMKK98 pKa = 11.31 LMMEE102 pKa = 4.38 KK103 pKa = 10.34 VDD105 pKa = 4.56 LLYY108 pKa = 11.09 ASTAVGTSAPMLPSHH123 pKa = 7.28 PAPPRR128 pKa = 11.84 IYY130 pKa = 9.39 PQLPSAPTTDD140 pKa = 3.14 EE141 pKa = 3.5 WDD143 pKa = 3.86 IIPP146 pKa = 4.79
Molecular weight: 16.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.701
IPC2_protein 4.609
IPC_protein 4.507
Toseland 4.355
ProMoST 4.609
Dawson 4.444
Bjellqvist 4.596
Wikipedia 4.317
Rodwell 4.342
Grimsley 4.266
Solomon 4.444
Lehninger 4.406
Nozaki 4.558
DTASelect 4.698
Thurlkill 4.355
EMBOSS 4.329
Sillero 4.622
Patrickios 4.164
IPC_peptide 4.457
IPC2_peptide 4.609
IPC2.peptide.svr19 4.575
Protein with the highest isoelectric point:
>sp|P0C797|NCAP_BDVV Nucleoprotein OS=Borna disease virus (strain V) OX=928296 GN=N PE=1 SV=1
MM1 pKa = 7.3 NSKK4 pKa = 10.31 HH5 pKa = 6.92 SYY7 pKa = 10.83 VEE9 pKa = 4.3 LKK11 pKa = 10.9 DD12 pKa = 3.76 KK13 pKa = 11.1 VIVPGWPTLMLEE25 pKa = 3.83 IDD27 pKa = 4.11 FVGGTSRR34 pKa = 11.84 NQFLNIPFLSVKK46 pKa = 10.28 EE47 pKa = 3.94 PLQLPRR53 pKa = 11.84 EE54 pKa = 4.28 KK55 pKa = 10.81 KK56 pKa = 8.75 LTDD59 pKa = 3.37 YY60 pKa = 8.76 FTIDD64 pKa = 3.51 VEE66 pKa = 4.47 PAGHH70 pKa = 6.21 SLVNIYY76 pKa = 9.79 FQIDD80 pKa = 3.82 DD81 pKa = 4.34 FLLLTLNSLSVYY93 pKa = 10.08 KK94 pKa = 10.8 DD95 pKa = 3.79 PIRR98 pKa = 11.84 KK99 pKa = 9.45 YY100 pKa = 10.2 MFLRR104 pKa = 11.84 LNKK107 pKa = 9.78 DD108 pKa = 3.36 QSKK111 pKa = 9.99 HH112 pKa = 5.94 AINAAFNVFSYY123 pKa = 10.67 RR124 pKa = 11.84 LRR126 pKa = 11.84 NIGVGPLGPDD136 pKa = 3.05 IRR138 pKa = 11.84 SSGPP142 pKa = 3.07
Molecular weight: 16.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.639
IPC2_protein 8.595
IPC_protein 8.624
Toseland 9.209
ProMoST 9.019
Dawson 9.472
Bjellqvist 9.151
Wikipedia 9.663
Rodwell 9.692
Grimsley 9.575
Solomon 9.589
Lehninger 9.545
Nozaki 9.121
DTASelect 9.165
Thurlkill 9.297
EMBOSS 9.619
Sillero 9.385
Patrickios 4.8
IPC_peptide 9.575
IPC2_peptide 7.702
IPC2.peptide.svr19 7.645
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
2
8
3517
87
1711
439.6
49.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.995 ± 0.565
2.019 ± 0.496
5.061 ± 0.351
5.601 ± 0.719
3.782 ± 0.495
5.886 ± 0.397
2.388 ± 0.2
6.028 ± 0.267
4.35 ± 0.511
11.373 ± 0.808
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.559 ± 0.488
2.985 ± 0.495
5.857 ± 0.561
3.583 ± 0.169
6.113 ± 0.366
8.274 ± 0.37
6.369 ± 0.647
6.17 ± 0.626
1.279 ± 0.257
3.327 ± 0.434
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here