Aspergillus ruber CBS 135680

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus ruber

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10053 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A017SEL8|A0A017SEL8_9EURO Serine/threonine-protein phosphatase 2A activator OS=Aspergillus ruber CBS 135680 OX=1388766 GN=EURHEDRAFT_402704 PE=3 SV=1
MM1 pKa = 7.86GYY3 pKa = 9.21ICQGWQLWSDD13 pKa = 3.44HH14 pKa = 6.49PFYY17 pKa = 9.94TQKK20 pKa = 10.93FIDD23 pKa = 3.81VDD25 pKa = 4.12VSSDD29 pKa = 2.98GYY31 pKa = 9.11TYY33 pKa = 10.62HH34 pKa = 7.57PSSQGEE40 pKa = 3.92IEE42 pKa = 3.87IDD44 pKa = 3.28EE45 pKa = 4.93DD46 pKa = 4.03VDD48 pKa = 5.66SEE50 pKa = 5.26DD51 pKa = 3.91EE52 pKa = 5.78DD53 pKa = 3.78EE54 pKa = 6.09GEE56 pKa = 5.48DD57 pKa = 4.4DD58 pKa = 6.21GEE60 pKa = 6.57DD61 pKa = 3.62GDD63 pKa = 4.08EE64 pKa = 4.93DD65 pKa = 4.01EE66 pKa = 4.88NEE68 pKa = 4.23EE69 pKa = 5.09YY70 pKa = 11.05VDD72 pKa = 3.49EE73 pKa = 5.26DD74 pKa = 4.2KK75 pKa = 11.9NNDD78 pKa = 2.99EE79 pKa = 5.1DD80 pKa = 4.78ANSSRR85 pKa = 11.84GIPDD89 pKa = 3.12ILSSYY94 pKa = 9.67PSAGQGYY101 pKa = 9.32KK102 pKa = 10.21RR103 pKa = 11.84RR104 pKa = 11.84DD105 pKa = 3.25SS106 pKa = 3.49

Molecular weight: 12.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A017SDB0|A0A017SDB0_9EURO DUF2235 domain-containing protein OS=Aspergillus ruber CBS 135680 OX=1388766 GN=EURHEDRAFT_516144 PE=4 SV=1
MM1 pKa = 7.52FSRR4 pKa = 11.84RR5 pKa = 11.84TLPALRR11 pKa = 11.84TLRR14 pKa = 11.84AQTPLTTTSLRR25 pKa = 11.84PFSSLLTSRR34 pKa = 11.84SSPPTTAITSTSTLLSPLTSTLTSAFNQSPSRR66 pKa = 11.84QFSASASLAGKK77 pKa = 10.07RR78 pKa = 11.84ITYY81 pKa = 9.51NPSRR85 pKa = 11.84RR86 pKa = 11.84VQKK89 pKa = 10.2RR90 pKa = 11.84RR91 pKa = 11.84HH92 pKa = 5.33GFLARR97 pKa = 11.84LRR99 pKa = 11.84SRR101 pKa = 11.84GGRR104 pKa = 11.84KK105 pKa = 8.5ILMRR109 pKa = 11.84RR110 pKa = 11.84RR111 pKa = 11.84QRR113 pKa = 11.84GKK115 pKa = 10.57KK116 pKa = 9.12ALSWW120 pKa = 3.66

Molecular weight: 13.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10053

0

10053

4591364

49

4894

456.7

50.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.076 ± 0.022

1.255 ± 0.01

5.651 ± 0.016

6.277 ± 0.023

3.806 ± 0.015

6.786 ± 0.019

2.435 ± 0.011

4.991 ± 0.017

4.756 ± 0.019

8.907 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.195 ± 0.009

3.891 ± 0.013

6.046 ± 0.027

4.165 ± 0.018

6.116 ± 0.019

8.361 ± 0.031

5.895 ± 0.016

6.116 ± 0.018

1.416 ± 0.01

2.859 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski