Vibrio phage phi 3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Ermolyevavirinae; Jesfedecavirus; Vibrio virus phi3

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 145 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5HAQ9|A0A0B5HAQ9_9CAUD Uncharacterized protein OS=Vibrio phage phi 3 OX=1589298 GN=SBVP3_0067 PE=4 SV=1
MM1 pKa = 7.45AKK3 pKa = 9.97EE4 pKa = 3.93INGYY8 pKa = 8.38MPVGMLQSLVVTIAYY23 pKa = 9.26KK24 pKa = 10.2PDD26 pKa = 3.49YY27 pKa = 11.14EE28 pKa = 4.51NTTSLADD35 pKa = 3.66EE36 pKa = 4.56MPSPEE41 pKa = 4.23TIVVSEE47 pKa = 4.31MYY49 pKa = 10.76VVDD52 pKa = 6.71FINQSQFYY60 pKa = 9.97LGLEE64 pKa = 4.06EE65 pKa = 6.03FVDD68 pKa = 4.51FDD70 pKa = 4.94KK71 pKa = 11.34EE72 pKa = 4.3PEE74 pKa = 4.24EE75 pKa = 4.01QCSAIAKK82 pKa = 9.72FIEE85 pKa = 4.82PKK87 pKa = 10.33PGFCPYY93 pKa = 9.83VVNAMFVDD101 pKa = 4.55FMGKK105 pKa = 8.66VQVIEE110 pKa = 5.09DD111 pKa = 4.71LDD113 pKa = 3.91AQSNFPSSYY122 pKa = 10.41SLKK125 pKa = 10.84

Molecular weight: 14.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5H309|A0A0B5H309_9CAUD Putative replicative DNA helicase OS=Vibrio phage phi 3 OX=1589298 GN=SBVP3_00110 PE=4 SV=1
MM1 pKa = 7.34TGKK4 pKa = 10.36KK5 pKa = 9.98LSARR9 pKa = 11.84TRR11 pKa = 11.84KK12 pKa = 9.54IEE14 pKa = 3.89GSFGFTLLEE23 pKa = 3.81ARR25 pKa = 11.84RR26 pKa = 11.84AGLTKK31 pKa = 10.66VLGLLSKK38 pKa = 10.25ATDD41 pKa = 4.5GYY43 pKa = 10.43VSKK46 pKa = 10.74HH47 pKa = 4.93PRR49 pKa = 11.84AVEE52 pKa = 3.78YY53 pKa = 8.49TASVFGLL60 pKa = 3.67

Molecular weight: 6.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

145

0

145

31785

48

1484

219.2

24.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.909 ± 0.351

1.158 ± 0.119

5.735 ± 0.158

6.83 ± 0.3

4.043 ± 0.147

6.358 ± 0.269

1.79 ± 0.125

5.808 ± 0.162

6.818 ± 0.269

8.998 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.131

5.009 ± 0.164

3.577 ± 0.133

3.184 ± 0.131

4.486 ± 0.166

7.161 ± 0.341

6.195 ± 0.316

7.224 ± 0.18

1.224 ± 0.068

4.172 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski