Amino acid dipepetide frequency for Sinorhizobium phage phiM9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.525AlaAla: 4.525 ± 0.327
0.626AlaCys: 0.626 ± 0.125
3.691AlaAsp: 3.691 ± 0.279
4.463AlaGlu: 4.463 ± 0.314
2.919AlaPhe: 2.919 ± 0.24
3.941AlaGly: 3.941 ± 0.304
1.376AlaHis: 1.376 ± 0.166
4.692AlaIle: 4.692 ± 0.344
5.005AlaLys: 5.005 ± 0.374
5.026AlaLeu: 5.026 ± 0.337
2.127AlaMet: 2.127 ± 0.207
2.669AlaAsn: 2.669 ± 0.217
2.252AlaPro: 2.252 ± 0.249
2.169AlaGln: 2.169 ± 0.227
3.774AlaArg: 3.774 ± 0.254
3.92AlaSer: 3.92 ± 0.337
3.274AlaThr: 3.274 ± 0.288
4.525AlaVal: 4.525 ± 0.288
0.938AlaTrp: 0.938 ± 0.113
2.148AlaTyr: 2.148 ± 0.227
0.0AlaXaa: 0.0 ± 0.0
Cys
0.542CysAla: 0.542 ± 0.123
0.104CysCys: 0.104 ± 0.046
0.772CysAsp: 0.772 ± 0.114
0.772CysGlu: 0.772 ± 0.126
0.605CysPhe: 0.605 ± 0.118
0.73CysGly: 0.73 ± 0.128
0.292CysHis: 0.292 ± 0.078
0.605CysIle: 0.605 ± 0.139
0.667CysLys: 0.667 ± 0.122
0.772CysLeu: 0.772 ± 0.126
0.188CysMet: 0.188 ± 0.066
0.438CysAsn: 0.438 ± 0.093
0.292CysPro: 0.292 ± 0.081
0.313CysGln: 0.313 ± 0.074
0.48CysArg: 0.48 ± 0.106
0.584CysSer: 0.584 ± 0.11
0.521CysThr: 0.521 ± 0.114
0.834CysVal: 0.834 ± 0.124
0.146CysTrp: 0.146 ± 0.05
0.438CysTyr: 0.438 ± 0.093
0.0CysXaa: 0.0 ± 0.0
Asp
4.671AspAla: 4.671 ± 0.299
0.48AspCys: 0.48 ± 0.093
4.212AspAsp: 4.212 ± 0.406
5.318AspGlu: 5.318 ± 0.402
3.545AspPhe: 3.545 ± 0.31
4.046AspGly: 4.046 ± 0.242
1.314AspHis: 1.314 ± 0.18
4.254AspIle: 4.254 ± 0.288
3.337AspLys: 3.337 ± 0.262
6.152AspLeu: 6.152 ± 0.362
1.919AspMet: 1.919 ± 0.207
2.44AspAsn: 2.44 ± 0.202
2.878AspPro: 2.878 ± 0.192
2.252AspGln: 2.252 ± 0.216
3.608AspArg: 3.608 ± 0.298
3.337AspSer: 3.337 ± 0.288
3.17AspThr: 3.17 ± 0.286
5.088AspVal: 5.088 ± 0.364
1.105AspTrp: 1.105 ± 0.147
2.899AspTyr: 2.899 ± 0.262
0.0AspXaa: 0.0 ± 0.0
Glu
4.692GluAla: 4.692 ± 0.379
0.959GluCys: 0.959 ± 0.17
4.108GluAsp: 4.108 ± 0.328
6.902GluGlu: 6.902 ± 0.641
4.254GluPhe: 4.254 ± 0.286
3.649GluGly: 3.649 ± 0.313
1.23GluHis: 1.23 ± 0.189
5.776GluIle: 5.776 ± 0.348
5.464GluLys: 5.464 ± 0.503
6.715GluLeu: 6.715 ± 0.416
1.981GluMet: 1.981 ± 0.174
3.754GluAsn: 3.754 ± 0.265
2.21GluPro: 2.21 ± 0.233
2.231GluGln: 2.231 ± 0.192
4.317GluArg: 4.317 ± 0.317
4.004GluSer: 4.004 ± 0.388
3.879GluThr: 3.879 ± 0.343
5.213GluVal: 5.213 ± 0.312
1.147GluTrp: 1.147 ± 0.16
2.899GluTyr: 2.899 ± 0.239
0.0GluXaa: 0.0 ± 0.0
Phe
2.899PheAla: 2.899 ± 0.26
0.563PheCys: 0.563 ± 0.098
4.129PheAsp: 4.129 ± 0.285
4.567PheGlu: 4.567 ± 0.313
2.231PhePhe: 2.231 ± 0.267
3.191PheGly: 3.191 ± 0.201
0.813PheHis: 0.813 ± 0.129
2.544PheIle: 2.544 ± 0.249
2.815PheLys: 2.815 ± 0.255
3.587PheLeu: 3.587 ± 0.264
1.46PheMet: 1.46 ± 0.182
2.419PheAsn: 2.419 ± 0.228
1.752PhePro: 1.752 ± 0.179
1.293PheGln: 1.293 ± 0.162
2.711PheArg: 2.711 ± 0.236
3.462PheSer: 3.462 ± 0.278
3.003PheThr: 3.003 ± 0.239
3.524PheVal: 3.524 ± 0.284
0.792PheTrp: 0.792 ± 0.13
1.981PheTyr: 1.981 ± 0.193
0.0PheXaa: 0.0 ± 0.0
Gly
3.608GlyAla: 3.608 ± 0.413
0.563GlyCys: 0.563 ± 0.113
4.192GlyAsp: 4.192 ± 0.341
3.879GlyGlu: 3.879 ± 0.247
2.836GlyPhe: 2.836 ± 0.201
4.192GlyGly: 4.192 ± 0.587
1.543GlyHis: 1.543 ± 0.184
3.733GlyIle: 3.733 ± 0.269
4.171GlyLys: 4.171 ± 0.329
4.629GlyLeu: 4.629 ± 0.374
1.522GlyMet: 1.522 ± 0.204
3.003GlyAsn: 3.003 ± 0.267
1.773GlyPro: 1.773 ± 0.183
2.127GlyGln: 2.127 ± 0.207
3.003GlyArg: 3.003 ± 0.28
4.4GlySer: 4.4 ± 0.377
4.692GlyThr: 4.692 ± 0.494
3.774GlyVal: 3.774 ± 0.26
0.938GlyTrp: 0.938 ± 0.143
2.586GlyTyr: 2.586 ± 0.26
0.0GlyXaa: 0.0 ± 0.0
His
1.105HisAla: 1.105 ± 0.161
0.271HisCys: 0.271 ± 0.079
1.022HisAsp: 1.022 ± 0.138
1.335HisGlu: 1.335 ± 0.197
0.918HisPhe: 0.918 ± 0.141
1.522HisGly: 1.522 ± 0.157
0.5HisHis: 0.5 ± 0.095
1.376HisIle: 1.376 ± 0.175
1.043HisLys: 1.043 ± 0.15
1.981HisLeu: 1.981 ± 0.257
0.5HisMet: 0.5 ± 0.095
0.834HisAsn: 0.834 ± 0.149
0.98HisPro: 0.98 ± 0.152
0.605HisGln: 0.605 ± 0.107
1.084HisArg: 1.084 ± 0.152
1.314HisSer: 1.314 ± 0.18
1.043HisThr: 1.043 ± 0.137
1.627HisVal: 1.627 ± 0.229
0.25HisTrp: 0.25 ± 0.079
0.772HisTyr: 0.772 ± 0.109
0.0HisXaa: 0.0 ± 0.0
Ile
4.212IleAla: 4.212 ± 0.292
0.772IleCys: 0.772 ± 0.136
5.38IleAsp: 5.38 ± 0.287
5.818IleGlu: 5.818 ± 0.391
2.773IlePhe: 2.773 ± 0.25
4.546IleGly: 4.546 ± 0.334
1.314IleHis: 1.314 ± 0.15
3.232IleIle: 3.232 ± 0.254
4.004IleLys: 4.004 ± 0.249
4.15IleLeu: 4.15 ± 0.35
1.272IleMet: 1.272 ± 0.172
3.211IleAsn: 3.211 ± 0.328
2.44IlePro: 2.44 ± 0.251
2.002IleGln: 2.002 ± 0.217
3.503IleArg: 3.503 ± 0.262
4.004IleSer: 4.004 ± 0.275
3.858IleThr: 3.858 ± 0.303
4.775IleVal: 4.775 ± 0.309
0.688IleTrp: 0.688 ± 0.114
2.336IleTyr: 2.336 ± 0.239
0.0IleXaa: 0.0 ± 0.0
Lys
4.483LysAla: 4.483 ± 0.295
0.688LysCys: 0.688 ± 0.124
4.504LysAsp: 4.504 ± 0.37
4.483LysGlu: 4.483 ± 0.313
3.733LysPhe: 3.733 ± 0.258
2.94LysGly: 2.94 ± 0.283
1.46LysHis: 1.46 ± 0.186
5.589LysIle: 5.589 ± 0.359
6.11LysLys: 6.11 ± 0.52
5.484LysLeu: 5.484 ± 0.312
2.106LysMet: 2.106 ± 0.224
3.483LysAsn: 3.483 ± 0.313
2.002LysPro: 2.002 ± 0.228
2.064LysGln: 2.064 ± 0.204
3.441LysArg: 3.441 ± 0.331
4.337LysSer: 4.337 ± 0.334
4.337LysThr: 4.337 ± 0.286
4.212LysVal: 4.212 ± 0.311
0.959LysTrp: 0.959 ± 0.147
2.315LysTyr: 2.315 ± 0.231
0.0LysXaa: 0.0 ± 0.0
Leu
5.484LeuAla: 5.484 ± 0.344
0.813LeuCys: 0.813 ± 0.135
5.484LeuAsp: 5.484 ± 0.345
6.569LeuGlu: 6.569 ± 0.386
3.42LeuPhe: 3.42 ± 0.293
4.525LeuGly: 4.525 ± 0.282
1.71LeuHis: 1.71 ± 0.163
4.212LeuIle: 4.212 ± 0.278
5.443LeuLys: 5.443 ± 0.398
5.881LeuLeu: 5.881 ± 0.423
1.939LeuMet: 1.939 ± 0.203
4.171LeuAsn: 4.171 ± 0.266
3.232LeuPro: 3.232 ± 0.262
2.69LeuGln: 2.69 ± 0.225
4.755LeuArg: 4.755 ± 0.366
5.756LeuSer: 5.756 ± 0.367
4.504LeuThr: 4.504 ± 0.359
5.255LeuVal: 5.255 ± 0.373
0.813LeuTrp: 0.813 ± 0.122
2.085LeuTyr: 2.085 ± 0.22
0.0LeuXaa: 0.0 ± 0.0
Met
2.023MetAla: 2.023 ± 0.199
0.188MetCys: 0.188 ± 0.062
1.209MetAsp: 1.209 ± 0.184
1.501MetGlu: 1.501 ± 0.169
1.314MetPhe: 1.314 ± 0.175
0.751MetGly: 0.751 ± 0.11
0.396MetHis: 0.396 ± 0.073
1.939MetIle: 1.939 ± 0.21
2.628MetLys: 2.628 ± 0.267
1.46MetLeu: 1.46 ± 0.199
0.813MetMet: 0.813 ± 0.127
1.481MetAsn: 1.481 ± 0.18
0.813MetPro: 0.813 ± 0.128
0.792MetGln: 0.792 ± 0.124
1.439MetArg: 1.439 ± 0.16
2.023MetSer: 2.023 ± 0.218
2.899MetThr: 2.899 ± 0.265
1.23MetVal: 1.23 ± 0.153
0.188MetTrp: 0.188 ± 0.058
0.834MetTyr: 0.834 ± 0.125
0.0MetXaa: 0.0 ± 0.0
Asn
3.211AsnAla: 3.211 ± 0.283
0.417AsnCys: 0.417 ± 0.113
3.003AsnAsp: 3.003 ± 0.231
3.483AsnGlu: 3.483 ± 0.285
2.794AsnPhe: 2.794 ± 0.256
4.15AsnGly: 4.15 ± 0.402
0.646AsnHis: 0.646 ± 0.128
2.773AsnIle: 2.773 ± 0.287
3.024AsnLys: 3.024 ± 0.246
3.524AsnLeu: 3.524 ± 0.34
1.23AsnMet: 1.23 ± 0.159
2.336AsnAsn: 2.336 ± 0.245
2.085AsnPro: 2.085 ± 0.199
1.418AsnGln: 1.418 ± 0.14
2.669AsnArg: 2.669 ± 0.225
3.128AsnSer: 3.128 ± 0.279
2.565AsnThr: 2.565 ± 0.242
3.503AsnVal: 3.503 ± 0.23
0.605AsnTrp: 0.605 ± 0.104
1.501AsnTyr: 1.501 ± 0.18
0.0AsnXaa: 0.0 ± 0.0
Pro
2.127ProAla: 2.127 ± 0.219
0.188ProCys: 0.188 ± 0.062
2.19ProAsp: 2.19 ± 0.2
2.836ProGlu: 2.836 ± 0.275
1.564ProPhe: 1.564 ± 0.193
2.377ProGly: 2.377 ± 0.213
0.792ProHis: 0.792 ± 0.146
2.19ProIle: 2.19 ± 0.222
2.377ProLys: 2.377 ± 0.25
2.648ProLeu: 2.648 ± 0.255
0.667ProMet: 0.667 ± 0.116
1.793ProAsn: 1.793 ± 0.159
1.043ProPro: 1.043 ± 0.181
0.918ProGln: 0.918 ± 0.165
1.773ProArg: 1.773 ± 0.184
2.669ProSer: 2.669 ± 0.246
1.981ProThr: 1.981 ± 0.204
3.211ProVal: 3.211 ± 0.295
0.292ProTrp: 0.292 ± 0.093
1.335ProTyr: 1.335 ± 0.177
0.0ProXaa: 0.0 ± 0.0
Gln
2.044GlnAla: 2.044 ± 0.184
0.271GlnCys: 0.271 ± 0.073
1.773GlnAsp: 1.773 ± 0.189
2.315GlnGlu: 2.315 ± 0.222
1.647GlnPhe: 1.647 ± 0.195
1.355GlnGly: 1.355 ± 0.148
0.646GlnHis: 0.646 ± 0.122
2.336GlnIle: 2.336 ± 0.238
2.169GlnLys: 2.169 ± 0.231
2.94GlnLeu: 2.94 ± 0.235
0.918GlnMet: 0.918 ± 0.153
1.314GlnAsn: 1.314 ± 0.185
0.709GlnPro: 0.709 ± 0.125
1.126GlnGln: 1.126 ± 0.145
1.585GlnArg: 1.585 ± 0.172
2.19GlnSer: 2.19 ± 0.293
1.439GlnThr: 1.439 ± 0.184
2.21GlnVal: 2.21 ± 0.27
0.375GlnTrp: 0.375 ± 0.091
1.209GlnTyr: 1.209 ± 0.161
0.0GlnXaa: 0.0 ± 0.0
Arg
3.42ArgAla: 3.42 ± 0.206
0.48ArgCys: 0.48 ± 0.087
3.17ArgAsp: 3.17 ± 0.269
3.649ArgGlu: 3.649 ± 0.247
2.836ArgPhe: 2.836 ± 0.279
3.086ArgGly: 3.086 ± 0.243
0.959ArgHis: 0.959 ± 0.159
3.774ArgIle: 3.774 ± 0.289
3.837ArgLys: 3.837 ± 0.286
4.65ArgLeu: 4.65 ± 0.312
1.501ArgMet: 1.501 ± 0.201
3.086ArgAsn: 3.086 ± 0.232
1.668ArgPro: 1.668 ± 0.195
1.877ArgGln: 1.877 ± 0.198
3.045ArgArg: 3.045 ± 0.31
3.608ArgSer: 3.608 ± 0.285
3.232ArgThr: 3.232 ± 0.252
3.962ArgVal: 3.962 ± 0.297
0.438ArgTrp: 0.438 ± 0.083
2.398ArgTyr: 2.398 ± 0.257
0.0ArgXaa: 0.0 ± 0.0
Ser
4.275SerAla: 4.275 ± 0.403
0.813SerCys: 0.813 ± 0.138
4.734SerAsp: 4.734 ± 0.355
4.817SerGlu: 4.817 ± 0.307
3.441SerPhe: 3.441 ± 0.256
5.109SerGly: 5.109 ± 0.533
1.418SerHis: 1.418 ± 0.172
4.233SerIle: 4.233 ± 0.275
4.358SerLys: 4.358 ± 0.33
5.151SerLeu: 5.151 ± 0.3
1.856SerMet: 1.856 ± 0.198
2.836SerAsn: 2.836 ± 0.235
1.939SerPro: 1.939 ± 0.208
1.668SerGln: 1.668 ± 0.154
3.545SerArg: 3.545 ± 0.253
5.38SerSer: 5.38 ± 0.419
3.733SerThr: 3.733 ± 0.382
4.838SerVal: 4.838 ± 0.347
0.772SerTrp: 0.772 ± 0.131
2.169SerTyr: 2.169 ± 0.186
0.0SerXaa: 0.0 ± 0.0
Thr
3.879ThrAla: 3.879 ± 0.318
0.605ThrCys: 0.605 ± 0.102
3.337ThrAsp: 3.337 ± 0.311
3.545ThrGlu: 3.545 ± 0.314
3.003ThrPhe: 3.003 ± 0.275
4.192ThrGly: 4.192 ± 0.534
0.876ThrHis: 0.876 ± 0.136
3.858ThrIle: 3.858 ± 0.246
3.795ThrLys: 3.795 ± 0.256
4.379ThrLeu: 4.379 ± 0.301
1.209ThrMet: 1.209 ± 0.152
3.003ThrAsn: 3.003 ± 0.343
2.461ThrPro: 2.461 ± 0.239
1.752ThrGln: 1.752 ± 0.205
3.42ThrArg: 3.42 ± 0.308
4.004ThrSer: 4.004 ± 0.518
4.212ThrThr: 4.212 ± 0.513
4.546ThrVal: 4.546 ± 0.368
1.064ThrTrp: 1.064 ± 0.143
2.482ThrTyr: 2.482 ± 0.235
0.0ThrXaa: 0.0 ± 0.0
Val
3.795ValAla: 3.795 ± 0.274
0.772ValCys: 0.772 ± 0.153
5.63ValAsp: 5.63 ± 0.467
5.61ValGlu: 5.61 ± 0.348
3.253ValPhe: 3.253 ± 0.222
4.025ValGly: 4.025 ± 0.385
1.606ValHis: 1.606 ± 0.185
4.233ValIle: 4.233 ± 0.284
5.026ValLys: 5.026 ± 0.325
5.568ValLeu: 5.568 ± 0.381
1.335ValMet: 1.335 ± 0.141
3.337ValAsn: 3.337 ± 0.253
2.586ValPro: 2.586 ± 0.3
1.814ValGln: 1.814 ± 0.173
3.962ValArg: 3.962 ± 0.304
5.568ValSer: 5.568 ± 0.489
4.463ValThr: 4.463 ± 0.335
5.067ValVal: 5.067 ± 0.413
0.751ValTrp: 0.751 ± 0.119
2.607ValTyr: 2.607 ± 0.243
0.0ValXaa: 0.0 ± 0.0
Trp
0.459TrpAla: 0.459 ± 0.096
0.209TrpCys: 0.209 ± 0.066
0.646TrpAsp: 0.646 ± 0.131
0.688TrpGlu: 0.688 ± 0.121
0.688TrpPhe: 0.688 ± 0.109
0.313TrpGly: 0.313 ± 0.08
0.375TrpHis: 0.375 ± 0.094
0.813TrpIle: 0.813 ± 0.123
1.355TrpLys: 1.355 ± 0.175
1.23TrpLeu: 1.23 ± 0.166
0.605TrpMet: 0.605 ± 0.122
0.751TrpAsn: 0.751 ± 0.131
0.48TrpPro: 0.48 ± 0.116
0.313TrpGln: 0.313 ± 0.075
0.772TrpArg: 0.772 ± 0.128
0.918TrpSer: 0.918 ± 0.165
1.001TrpThr: 1.001 ± 0.133
0.605TrpVal: 0.605 ± 0.094
0.355TrpTrp: 0.355 ± 0.1
0.563TrpTyr: 0.563 ± 0.102
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.607TyrAla: 2.607 ± 0.203
0.313TyrCys: 0.313 ± 0.073
2.836TyrAsp: 2.836 ± 0.267
2.836TyrGlu: 2.836 ± 0.237
1.96TyrPhe: 1.96 ± 0.223
2.523TyrGly: 2.523 ± 0.295
0.813TyrHis: 0.813 ± 0.138
2.127TyrIle: 2.127 ± 0.194
2.106TyrLys: 2.106 ± 0.244
2.815TyrLeu: 2.815 ± 0.206
0.751TyrMet: 0.751 ± 0.125
1.71TyrAsn: 1.71 ± 0.159
1.376TyrPro: 1.376 ± 0.162
1.147TyrGln: 1.147 ± 0.152
1.793TyrArg: 1.793 ± 0.197
2.482TyrSer: 2.482 ± 0.245
1.814TyrThr: 1.814 ± 0.282
3.024TyrVal: 3.024 ± 0.292
0.521TyrTrp: 0.521 ± 0.101
1.084TyrTyr: 1.084 ± 0.149
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 271 proteins (47955 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski