Amino acid dipepetide frequency for Lactobacillus nasuensis JCM 17158

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.021AlaAla: 16.021 ± 0.305	0.415AlaCys: 0.415 ± 0.025	6.869AlaAsp: 6.869 ± 0.112	5.445AlaGlu: 5.445 ± 0.095	3.711AlaPhe: 3.711 ± 0.075	9.365AlaGly: 9.365 ± 0.151	2.467AlaHis: 2.467 ± 0.062	6.443AlaIle: 6.443 ± 0.118	6.679AlaLys: 6.679 ± 0.127	11.316AlaLeu: 11.316 ± 0.16	2.957AlaMet: 2.957 ± 0.065	4.063AlaAsn: 4.063 ± 0.101	4.55AlaPro: 4.55 ± 0.105	5.446AlaGln: 5.446 ± 0.112	4.517AlaArg: 4.517 ± 0.103	5.824AlaSer: 5.824 ± 0.204	8.893AlaThr: 8.893 ± 0.196	8.759AlaVal: 8.759 ± 0.125	1.26AlaTrp: 1.26 ± 0.044	3.239AlaTyr: 3.239 ± 0.067	0.002AlaXaa: 0.002 ± 0.002
Cys	0.4CysAla: 0.4 ± 0.026	0.035CysCys: 0.035 ± 0.008	0.21CysAsp: 0.21 ± 0.017	0.166CysGlu: 0.166 ± 0.017	0.199CysPhe: 0.199 ± 0.018	0.446CysGly: 0.446 ± 0.027	0.128CysHis: 0.128 ± 0.014	0.172CysIle: 0.172 ± 0.014	0.09CysLys: 0.09 ± 0.012	0.421CysLeu: 0.421 ± 0.026	0.075CysMet: 0.075 ± 0.012	0.125CysAsn: 0.125 ± 0.015	0.179CysPro: 0.179 ± 0.021	0.137CysGln: 0.137 ± 0.015	0.157CysArg: 0.157 ± 0.015	0.196CysSer: 0.196 ± 0.017	0.199CysThr: 0.199 ± 0.015	0.274CysVal: 0.274 ± 0.026	0.06CysTrp: 0.06 ± 0.009	0.13CysTyr: 0.13 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	6.239AspAla: 6.239 ± 0.118	0.219AspCys: 0.219 ± 0.019	3.316AspAsp: 3.316 ± 0.095	2.872AspGlu: 2.872 ± 0.08	2.568AspPhe: 2.568 ± 0.06	3.956AspGly: 3.956 ± 0.121	1.46AspHis: 1.46 ± 0.05	2.961AspIle: 2.961 ± 0.076	2.256AspLys: 2.256 ± 0.063	5.482AspLeu: 5.482 ± 0.115	1.487AspMet: 1.487 ± 0.048	1.87AspAsn: 1.87 ± 0.051	2.886AspPro: 2.886 ± 0.073	2.548AspGln: 2.548 ± 0.069	2.631AspArg: 2.631 ± 0.066	2.614AspSer: 2.614 ± 0.071	3.435AspThr: 3.435 ± 0.074	3.896AspVal: 3.896 ± 0.073	0.998AspTrp: 0.998 ± 0.043	2.572AspTyr: 2.572 ± 0.067	0.0AspXaa: 0.0 ± 0.0
Glu	5.264GluAla: 5.264 ± 0.109	0.164GluCys: 0.164 ± 0.016	2.042GluAsp: 2.042 ± 0.066	2.133GluGlu: 2.133 ± 0.073	1.779GluPhe: 1.779 ± 0.053	2.194GluGly: 2.194 ± 0.064	1.122GluHis: 1.122 ± 0.047	2.712GluIle: 2.712 ± 0.085	1.977GluLys: 1.977 ± 0.067	5.152GluLeu: 5.152 ± 0.094	1.301GluMet: 1.301 ± 0.05	1.63GluAsn: 1.63 ± 0.053	1.948GluPro: 1.948 ± 0.054	2.337GluGln: 2.337 ± 0.069	2.314GluArg: 2.314 ± 0.065	2.055GluSer: 2.055 ± 0.064	2.842GluThr: 2.842 ± 0.068	2.896GluVal: 2.896 ± 0.076	0.479GluTrp: 0.479 ± 0.028	1.41GluTyr: 1.41 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	4.428PheAla: 4.428 ± 0.081	0.195PheCys: 0.195 ± 0.018	2.575PheAsp: 2.575 ± 0.054	1.583PheGlu: 1.583 ± 0.055	1.559PhePhe: 1.559 ± 0.053	3.395PheGly: 3.395 ± 0.073	0.795PheHis: 0.795 ± 0.036	2.26PheIle: 2.26 ± 0.063	1.577PheLys: 1.577 ± 0.051	3.236PheLeu: 3.236 ± 0.084	0.957PheMet: 0.957 ± 0.037	1.597PheAsn: 1.597 ± 0.054	1.42PhePro: 1.42 ± 0.052	1.227PheGln: 1.227 ± 0.047	1.389PheArg: 1.389 ± 0.042	2.378PheSer: 2.378 ± 0.067	3.091PheThr: 3.091 ± 0.071	2.801PheVal: 2.801 ± 0.074	0.493PheTrp: 0.493 ± 0.026	1.295PheTyr: 1.295 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	7.135GlyAla: 7.135 ± 0.172	0.359GlyCys: 0.359 ± 0.023	4.241GlyAsp: 4.241 ± 0.094	3.54GlyGlu: 3.54 ± 0.086	3.123GlyPhe: 3.123 ± 0.076	5.371GlyGly: 5.371 ± 0.116	1.72GlyHis: 1.72 ± 0.045	4.768GlyIle: 4.768 ± 0.094	3.849GlyLys: 3.849 ± 0.091	7.519GlyLeu: 7.519 ± 0.105	1.962GlyMet: 1.962 ± 0.055	2.51GlyAsn: 2.51 ± 0.074	2.13GlyPro: 2.13 ± 0.06	3.346GlyGln: 3.346 ± 0.076	3.322GlyArg: 3.322 ± 0.071	3.901GlySer: 3.901 ± 0.096	5.16GlyThr: 5.16 ± 0.118	5.864GlyVal: 5.864 ± 0.103	1.042GlyTrp: 1.042 ± 0.05	2.886GlyTyr: 2.886 ± 0.075	0.003GlyXaa: 0.003 ± 0.002
His	2.25HisAla: 2.25 ± 0.063	0.106HisCys: 0.106 ± 0.014	1.547HisAsp: 1.547 ± 0.045	1.126HisGlu: 1.126 ± 0.042	1.083HisPhe: 1.083 ± 0.039	1.794HisGly: 1.794 ± 0.051	0.796HisHis: 0.796 ± 0.042	1.2HisIle: 1.2 ± 0.042	0.71HisLys: 0.71 ± 0.032	2.509HisLeu: 2.509 ± 0.073	0.532HisMet: 0.532 ± 0.029	0.728HisAsn: 0.728 ± 0.035	1.254HisPro: 1.254 ± 0.049	1.181HisGln: 1.181 ± 0.043	1.238HisArg: 1.238 ± 0.049	1.01HisSer: 1.01 ± 0.04	1.346HisThr: 1.346 ± 0.053	1.639HisVal: 1.639 ± 0.045	0.338HisTrp: 0.338 ± 0.022	1.004HisTyr: 1.004 ± 0.039	0.0HisXaa: 0.0 ± 0.0
Ile	6.651IleAla: 6.651 ± 0.112	0.274IleCys: 0.274 ± 0.02	3.786IleAsp: 3.786 ± 0.088	2.501IleGlu: 2.501 ± 0.07	1.927IlePhe: 1.927 ± 0.06	4.953IleGly: 4.953 ± 0.114	1.212IleHis: 1.212 ± 0.047	3.325IleIle: 3.325 ± 0.104	2.336IleLys: 2.336 ± 0.073	4.676IleLeu: 4.676 ± 0.087	1.381IleMet: 1.381 ± 0.05	2.281IleAsn: 2.281 ± 0.063	2.339IlePro: 2.339 ± 0.061	2.152IleGln: 2.152 ± 0.048	2.342IleArg: 2.342 ± 0.066	2.878IleSer: 2.878 ± 0.079	4.219IleThr: 4.219 ± 0.08	4.293IleVal: 4.293 ± 0.09	0.528IleTrp: 0.528 ± 0.032	1.594IleTyr: 1.594 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	5.167LysAla: 5.167 ± 0.095	0.08LysCys: 0.08 ± 0.012	2.334LysAsp: 2.334 ± 0.076	1.936LysGlu: 1.936 ± 0.072	1.404LysPhe: 1.404 ± 0.045	2.544LysGly: 2.544 ± 0.076	1.021LysHis: 1.021 ± 0.042	2.308LysIle: 2.308 ± 0.077	2.25LysLys: 2.25 ± 0.086	4.605LysLeu: 4.605 ± 0.093	1.3LysMet: 1.3 ± 0.046	1.66LysAsn: 1.66 ± 0.049	2.084LysPro: 2.084 ± 0.066	2.66LysGln: 2.66 ± 0.066	2.518LysArg: 2.518 ± 0.073	2.155LysSer: 2.155 ± 0.066	3.165LysThr: 3.165 ± 0.085	2.985LysVal: 2.985 ± 0.08	0.511LysTrp: 0.511 ± 0.028	1.395LysTyr: 1.395 ± 0.055	0.0LysXaa: 0.0 ± 0.0
Leu	12.777LeuAla: 12.777 ± 0.196	0.431LeuCys: 0.431 ± 0.028	5.196LeuAsp: 5.196 ± 0.103	3.533LeuGlu: 3.533 ± 0.086	3.665LeuPhe: 3.665 ± 0.083	7.43LeuGly: 7.43 ± 0.118	2.156LeuHis: 2.156 ± 0.061	5.788LeuIle: 5.788 ± 0.12	4.166LeuLys: 4.166 ± 0.093	9.457LeuLeu: 9.457 ± 0.137	2.535LeuMet: 2.535 ± 0.068	3.759LeuAsn: 3.759 ± 0.083	5.262LeuPro: 5.262 ± 0.101	4.122LeuGln: 4.122 ± 0.092	4.814LeuArg: 4.814 ± 0.102	5.407LeuSer: 5.407 ± 0.115	8.54LeuThr: 8.54 ± 0.14	7.498LeuVal: 7.498 ± 0.112	1.096LeuTrp: 1.096 ± 0.042	2.66LeuTyr: 2.66 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	2.945MetAla: 2.945 ± 0.074	0.086MetCys: 0.086 ± 0.01	1.179MetAsp: 1.179 ± 0.048	0.784MetGlu: 0.784 ± 0.039	0.876MetPhe: 0.876 ± 0.04	1.597MetGly: 1.597 ± 0.056	0.525MetHis: 0.525 ± 0.029	1.447MetIle: 1.447 ± 0.047	1.417MetLys: 1.417 ± 0.048	2.473MetLeu: 2.473 ± 0.069	0.721MetMet: 0.721 ± 0.034	1.095MetAsn: 1.095 ± 0.043	1.117MetPro: 1.117 ± 0.037	1.238MetGln: 1.238 ± 0.043	1.26MetArg: 1.26 ± 0.044	1.425MetSer: 1.425 ± 0.051	2.094MetThr: 2.094 ± 0.061	1.796MetVal: 1.796 ± 0.049	0.195MetTrp: 0.195 ± 0.017	0.576MetTyr: 0.576 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	3.742AsnAla: 3.742 ± 0.083	0.131AsnCys: 0.131 ± 0.014	1.957AsnAsp: 1.957 ± 0.057	1.634AsnGlu: 1.634 ± 0.058	1.38AsnPhe: 1.38 ± 0.051	3.121AsnGly: 3.121 ± 0.098	1.0AsnHis: 1.0 ± 0.043	1.808AsnIle: 1.808 ± 0.052	1.377AsnLys: 1.377 ± 0.056	3.394AsnLeu: 3.394 ± 0.074	0.982AsnMet: 0.982 ± 0.044	1.197AsnAsn: 1.197 ± 0.047	1.861AsnPro: 1.861 ± 0.059	1.901AsnGln: 1.901 ± 0.054	1.852AsnArg: 1.852 ± 0.057	1.645AsnSer: 1.645 ± 0.049	2.206AsnThr: 2.206 ± 0.063	2.542AsnVal: 2.542 ± 0.071	0.541AsnTrp: 0.541 ± 0.027	1.28AsnTyr: 1.28 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	6.137ProAla: 6.137 ± 0.12	0.109ProCys: 0.109 ± 0.013	2.868ProAsp: 2.868 ± 0.075	2.715ProGlu: 2.715 ± 0.078	1.618ProPhe: 1.618 ± 0.06	3.07ProGly: 3.07 ± 0.08	0.995ProHis: 0.995 ± 0.046	2.213ProIle: 2.213 ± 0.058	1.858ProLys: 1.858 ± 0.059	4.13ProLeu: 4.13 ± 0.089	0.856ProMet: 0.856 ± 0.039	1.594ProAsn: 1.594 ± 0.052	1.036ProPro: 1.036 ± 0.044	2.082ProGln: 2.082 ± 0.067	1.591ProArg: 1.591 ± 0.048	2.277ProSer: 2.277 ± 0.064	3.548ProThr: 3.548 ± 0.085	3.754ProVal: 3.754 ± 0.077	0.49ProTrp: 0.49 ± 0.027	1.343ProTyr: 1.343 ± 0.046	0.002ProXaa: 0.002 ± 0.001
Gln	6.367GlnAla: 6.367 ± 0.137	0.109GlnCys: 0.109 ± 0.011	1.999GlnAsp: 1.999 ± 0.058	1.766GlnGlu: 1.766 ± 0.053	1.805GlnPhe: 1.805 ± 0.051	2.399GlnGly: 2.399 ± 0.062	1.156GlnHis: 1.156 ± 0.049	2.334GlnIle: 2.334 ± 0.06	1.71GlnLys: 1.71 ± 0.051	5.669GlnLeu: 5.669 ± 0.108	1.096GlnMet: 1.096 ± 0.045	1.417GlnAsn: 1.417 ± 0.046	2.605GlnPro: 2.605 ± 0.07	2.697GlnGln: 2.697 ± 0.07	2.296GlnArg: 2.296 ± 0.067	2.26GlnSer: 2.26 ± 0.086	3.311GlnThr: 3.311 ± 0.078	3.575GlnVal: 3.575 ± 0.077	0.561GlnTrp: 0.561 ± 0.032	1.58GlnTyr: 1.58 ± 0.05	0.0GlnXaa: 0.0 ± 0.0
Arg	4.419ArgAla: 4.419 ± 0.091	0.145ArgCys: 0.145 ± 0.015	2.611ArgAsp: 2.611 ± 0.066	2.515ArgGlu: 2.515 ± 0.074	2.023ArgPhe: 2.023 ± 0.062	2.928ArgGly: 2.928 ± 0.08	1.378ArgHis: 1.378 ± 0.048	2.44ArgIle: 2.44 ± 0.072	1.836ArgLys: 1.836 ± 0.058	5.28ArgLeu: 5.28 ± 0.118	1.086ArgMet: 1.086 ± 0.042	1.42ArgAsn: 1.42 ± 0.045	1.963ArgPro: 1.963 ± 0.053	2.921ArgGln: 2.921 ± 0.075	3.121ArgArg: 3.121 ± 0.084	1.919ArgSer: 1.919 ± 0.062	2.545ArgThr: 2.545 ± 0.072	3.498ArgVal: 3.498 ± 0.071	0.579ArgTrp: 0.579 ± 0.032	1.838ArgTyr: 1.838 ± 0.062	0.0ArgXaa: 0.0 ± 0.0
Ser	5.71SerAla: 5.71 ± 0.18	0.16SerCys: 0.16 ± 0.017	3.086SerAsp: 3.086 ± 0.079	2.316SerGlu: 2.316 ± 0.071	2.241SerPhe: 2.241 ± 0.063	4.543SerGly: 4.543 ± 0.097	1.114SerHis: 1.114 ± 0.045	2.557SerIle: 2.557 ± 0.072	2.042SerLys: 2.042 ± 0.068	5.08SerLeu: 5.08 ± 0.11	1.149SerMet: 1.149 ± 0.045	1.675SerAsn: 1.675 ± 0.058	1.992SerPro: 1.992 ± 0.055	2.301SerGln: 2.301 ± 0.094	2.299SerArg: 2.299 ± 0.067	2.654SerSer: 2.654 ± 0.107	3.481SerThr: 3.481 ± 0.149	3.828SerVal: 3.828 ± 0.094	0.692SerTrp: 0.692 ± 0.036	1.672SerTyr: 1.672 ± 0.058	0.0SerXaa: 0.0 ± 0.0
Thr	8.493ThrAla: 8.493 ± 0.172	0.188ThrCys: 0.188 ± 0.017	3.833ThrAsp: 3.833 ± 0.085	2.749ThrGlu: 2.749 ± 0.066	2.55ThrPhe: 2.55 ± 0.07	6.036ThrGly: 6.036 ± 0.152	1.533ThrHis: 1.533 ± 0.053	4.424ThrIle: 4.424 ± 0.096	3.481ThrLys: 3.481 ± 0.087	7.282ThrLeu: 7.282 ± 0.127	1.535ThrMet: 1.535 ± 0.051	2.551ThrAsn: 2.551 ± 0.073	4.077ThrPro: 4.077 ± 0.099	2.97ThrGln: 2.97 ± 0.08	2.854ThrArg: 2.854 ± 0.066	3.665ThrSer: 3.665 ± 0.145	5.821ThrThr: 5.821 ± 0.194	6.318ThrVal: 6.318 ± 0.114	0.784ThrTrp: 0.784 ± 0.04	2.283ThrTyr: 2.283 ± 0.065	0.002ThrXaa: 0.002 ± 0.001
Val	9.695ValAla: 9.695 ± 0.148	0.341ValCys: 0.341 ± 0.026	4.098ValAsp: 4.098 ± 0.075	2.738ValGlu: 2.738 ± 0.084	2.648ValPhe: 2.648 ± 0.069	5.478ValGly: 5.478 ± 0.104	1.437ValHis: 1.437 ± 0.046	4.47ValIle: 4.47 ± 0.11	3.212ValLys: 3.212 ± 0.084	6.993ValLeu: 6.993 ± 0.129	2.017ValMet: 2.017 ± 0.06	2.823ValAsn: 2.823 ± 0.065	3.579ValPro: 3.579 ± 0.072	2.731ValGln: 2.731 ± 0.069	3.175ValArg: 3.175 ± 0.085	4.161ValSer: 4.161 ± 0.093	6.67ValThr: 6.67 ± 0.119	6.343ValVal: 6.343 ± 0.141	0.841ValTrp: 0.841 ± 0.037	2.209ValTyr: 2.209 ± 0.053	0.002ValXaa: 0.002 ± 0.001
Trp	1.037TrpAla: 1.037 ± 0.041	0.075TrpCys: 0.075 ± 0.009	0.496TrpAsp: 0.496 ± 0.03	0.35TrpGlu: 0.35 ± 0.025	0.6TrpPhe: 0.6 ± 0.032	0.805TrpGly: 0.805 ± 0.035	0.416TrpHis: 0.416 ± 0.023	0.473TrpIle: 0.473 ± 0.03	0.253TrpLys: 0.253 ± 0.021	2.037TrpLeu: 2.037 ± 0.074	0.231TrpMet: 0.231 ± 0.02	0.404TrpAsn: 0.404 ± 0.031	0.508TrpPro: 0.508 ± 0.029	0.911TrpGln: 0.911 ± 0.033	0.835TrpArg: 0.835 ± 0.038	0.582TrpSer: 0.582 ± 0.03	0.657TrpThr: 0.657 ± 0.035	0.835TrpVal: 0.835 ± 0.042	0.305TrpTrp: 0.305 ± 0.024	0.404TrpTyr: 0.404 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.364TyrAla: 3.364 ± 0.069	0.178TyrCys: 0.178 ± 0.017	2.022TyrAsp: 2.022 ± 0.061	1.349TyrGlu: 1.349 ± 0.054	1.496TyrPhe: 1.496 ± 0.049	2.325TyrGly: 2.325 ± 0.069	0.96TyrHis: 0.96 ± 0.038	1.539TyrIle: 1.539 ± 0.049	1.004TyrLys: 1.004 ± 0.041	3.581TyrLeu: 3.581 ± 0.081	0.685TyrMet: 0.685 ± 0.03	1.149TyrAsn: 1.149 ± 0.046	1.491TyrPro: 1.491 ± 0.053	1.806TyrGln: 1.806 ± 0.053	1.94TyrArg: 1.94 ± 0.06	1.586TyrSer: 1.586 ± 0.052	2.204TyrThr: 2.204 ± 0.074	2.266TyrVal: 2.266 ± 0.058	0.422TyrTrp: 0.422 ± 0.028	1.253TyrTyr: 1.253 ± 0.056	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.002XaaCys: 0.002 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.001	0.002XaaPro: 0.002 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.002XaaThr: 0.002 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.149XaaXaa: 0.149 ± 0.072

Statistics based on 2092 proteins (663238 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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