Amino acid dipepetide frequency for Streptomyces silvensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.98AlaAla: 22.98 ± 0.155	1.123AlaCys: 1.123 ± 0.021	9.059AlaAsp: 9.059 ± 0.072	8.746AlaGlu: 8.746 ± 0.067	3.515AlaPhe: 3.515 ± 0.037	13.373AlaGly: 13.373 ± 0.094	3.216AlaHis: 3.216 ± 0.045	3.092AlaIle: 3.092 ± 0.046	2.892AlaLys: 2.892 ± 0.053	14.728AlaLeu: 14.728 ± 0.111	2.411AlaMet: 2.411 ± 0.033	1.781AlaAsn: 1.781 ± 0.031	7.92AlaPro: 7.92 ± 0.087	3.682AlaGln: 3.682 ± 0.049	11.15AlaArg: 11.15 ± 0.079	5.722AlaSer: 5.722 ± 0.053	7.017AlaThr: 7.017 ± 0.055	12.424AlaVal: 12.424 ± 0.083	1.882AlaTrp: 1.882 ± 0.029	2.84AlaTyr: 2.84 ± 0.04	0.005AlaXaa: 0.005 ± 0.001
Cys	1.171CysAla: 1.171 ± 0.023	0.086CysCys: 0.086 ± 0.006	0.485CysAsp: 0.485 ± 0.015	0.394CysGlu: 0.394 ± 0.012	0.222CysPhe: 0.222 ± 0.009	0.983CysGly: 0.983 ± 0.022	0.203CysHis: 0.203 ± 0.01	0.132CysIle: 0.132 ± 0.007	0.115CysLys: 0.115 ± 0.007	0.771CysLeu: 0.771 ± 0.019	0.126CysMet: 0.126 ± 0.007	0.116CysAsn: 0.116 ± 0.007	0.498CysPro: 0.498 ± 0.015	0.171CysGln: 0.171 ± 0.008	0.639CysArg: 0.639 ± 0.021	0.402CysSer: 0.402 ± 0.012	0.507CysThr: 0.507 ± 0.015	0.744CysVal: 0.744 ± 0.017	0.125CysTrp: 0.125 ± 0.007	0.154CysTyr: 0.154 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	8.278AspAla: 8.278 ± 0.07	0.427AspCys: 0.427 ± 0.014	3.945AspAsp: 3.945 ± 0.047	3.927AspGlu: 3.927 ± 0.038	1.699AspPhe: 1.699 ± 0.025	6.672AspGly: 6.672 ± 0.064	1.471AspHis: 1.471 ± 0.027	1.929AspIle: 1.929 ± 0.031	1.355AspLys: 1.355 ± 0.028	6.458AspLeu: 6.458 ± 0.057	0.814AspMet: 0.814 ± 0.018	0.922AspAsn: 0.922 ± 0.023	4.464AspPro: 4.464 ± 0.047	1.508AspGln: 1.508 ± 0.026	5.132AspArg: 5.132 ± 0.053	2.558AspSer: 2.558 ± 0.035	3.432AspThr: 3.432 ± 0.031	5.012AspVal: 5.012 ± 0.048	0.999AspTrp: 0.999 ± 0.022	1.106AspTyr: 1.106 ± 0.024	0.004AspXaa: 0.004 ± 0.001
Glu	7.2GluAla: 7.2 ± 0.07	0.384GluCys: 0.384 ± 0.012	2.795GluAsp: 2.795 ± 0.035	3.339GluGlu: 3.339 ± 0.047	1.423GluPhe: 1.423 ± 0.026	4.346GluGly: 4.346 ± 0.045	1.57GluHis: 1.57 ± 0.026	2.073GluIle: 2.073 ± 0.031	1.433GluLys: 1.433 ± 0.028	6.645GluLeu: 6.645 ± 0.063	0.814GluMet: 0.814 ± 0.02	0.961GluAsn: 0.961 ± 0.022	3.372GluPro: 3.372 ± 0.041	2.297GluGln: 2.297 ± 0.037	5.797GluArg: 5.797 ± 0.054	2.596GluSer: 2.596 ± 0.033	2.761GluThr: 2.761 ± 0.03	4.309GluVal: 4.309 ± 0.045	0.761GluTrp: 0.761 ± 0.02	1.066GluTyr: 1.066 ± 0.024	0.003GluXaa: 0.003 ± 0.001
Phe	3.735PheAla: 3.735 ± 0.042	0.256PheCys: 0.256 ± 0.01	1.999PheAsp: 1.999 ± 0.028	1.391PheGlu: 1.391 ± 0.024	0.827PhePhe: 0.827 ± 0.021	2.945PheGly: 2.945 ± 0.034	0.615PheHis: 0.615 ± 0.017	0.638PheIle: 0.638 ± 0.018	0.523PheLys: 0.523 ± 0.015	2.531PheLeu: 2.531 ± 0.031	0.392PheMet: 0.392 ± 0.014	0.529PheAsn: 0.529 ± 0.014	1.368PhePro: 1.368 ± 0.026	0.642PheGln: 0.642 ± 0.018	1.85PheArg: 1.85 ± 0.026	1.369PheSer: 1.369 ± 0.025	1.977PheThr: 1.977 ± 0.032	2.231PheVal: 2.231 ± 0.035	0.414PheTrp: 0.414 ± 0.014	0.53PheTyr: 0.53 ± 0.015	0.001PheXaa: 0.001 ± 0.001
Gly	12.087GlyAla: 12.087 ± 0.101	0.844GlyCys: 0.844 ± 0.018	5.578GlyAsp: 5.578 ± 0.052	5.195GlyGlu: 5.195 ± 0.051	2.766GlyPhe: 2.766 ± 0.036	9.4GlyGly: 9.4 ± 0.1	2.398GlyHis: 2.398 ± 0.034	3.164GlyIle: 3.164 ± 0.04	2.557GlyLys: 2.557 ± 0.046	9.166GlyLeu: 9.166 ± 0.061	1.861GlyMet: 1.861 ± 0.028	1.701GlyAsn: 1.701 ± 0.032	5.57GlyPro: 5.57 ± 0.054	2.565GlyGln: 2.565 ± 0.038	8.018GlyArg: 8.018 ± 0.069	5.405GlySer: 5.405 ± 0.054	6.643GlyThr: 6.643 ± 0.067	7.616GlyVal: 7.616 ± 0.058	1.623GlyTrp: 1.623 ± 0.027	2.216GlyTyr: 2.216 ± 0.032	0.008GlyXaa: 0.008 ± 0.002
His	2.899HisAla: 2.899 ± 0.036	0.229HisCys: 0.229 ± 0.011	1.473HisAsp: 1.473 ± 0.025	1.303HisGlu: 1.303 ± 0.025	0.668HisPhe: 0.668 ± 0.018	2.547HisGly: 2.547 ± 0.034	0.765HisHis: 0.765 ± 0.02	0.683HisIle: 0.683 ± 0.018	0.379HisLys: 0.379 ± 0.01	2.584HisLeu: 2.584 ± 0.032	0.344HisMet: 0.344 ± 0.011	0.353HisAsn: 0.353 ± 0.011	1.894HisPro: 1.894 ± 0.027	0.7HisGln: 0.7 ± 0.02	2.279HisArg: 2.279 ± 0.033	1.042HisSer: 1.042 ± 0.023	1.458HisThr: 1.458 ± 0.025	1.752HisVal: 1.752 ± 0.028	0.387HisTrp: 0.387 ± 0.013	0.485HisTyr: 0.485 ± 0.014	0.002HisXaa: 0.002 ± 0.001
Ile	4.336IleAla: 4.336 ± 0.042	0.277IleCys: 0.277 ± 0.01	2.05IleAsp: 2.05 ± 0.032	1.832IleGlu: 1.832 ± 0.029	0.638IlePhe: 0.638 ± 0.018	3.299IleGly: 3.299 ± 0.034	0.593IleHis: 0.593 ± 0.018	0.721IleIle: 0.721 ± 0.017	0.705IleLys: 0.705 ± 0.021	2.211IleLeu: 2.211 ± 0.032	0.402IleMet: 0.402 ± 0.012	0.613IleAsn: 0.613 ± 0.017	1.571IlePro: 1.571 ± 0.028	0.641IleGln: 0.641 ± 0.016	2.06IleArg: 2.06 ± 0.028	1.475IleSer: 1.475 ± 0.029	1.991IleThr: 1.991 ± 0.029	2.477IleVal: 2.477 ± 0.039	0.335IleTrp: 0.335 ± 0.012	0.467IleTyr: 0.467 ± 0.013	0.002IleXaa: 0.002 ± 0.001
Lys	2.989LysAla: 2.989 ± 0.046	0.125LysCys: 0.125 ± 0.007	1.482LysAsp: 1.482 ± 0.033	1.266LysGlu: 1.266 ± 0.027	0.502LysPhe: 0.502 ± 0.014	1.929LysGly: 1.929 ± 0.038	0.44LysHis: 0.44 ± 0.015	0.836LysIle: 0.836 ± 0.023	0.996LysLys: 0.996 ± 0.03	1.981LysLeu: 1.981 ± 0.033	0.37LysMet: 0.37 ± 0.012	0.537LysAsn: 0.537 ± 0.017	1.399LysPro: 1.399 ± 0.032	0.718LysGln: 0.718 ± 0.021	1.468LysArg: 1.468 ± 0.027	1.225LysSer: 1.225 ± 0.029	1.196LysThr: 1.196 ± 0.025	1.869LysVal: 1.869 ± 0.031	0.28LysTrp: 0.28 ± 0.01	0.443LysTyr: 0.443 ± 0.016	0.001LysXaa: 0.001 ± 0.001
Leu	15.075LeuAla: 15.075 ± 0.107	0.902LeuCys: 0.902 ± 0.018	6.808LeuAsp: 6.808 ± 0.059	4.379LeuGlu: 4.379 ± 0.048	2.591LeuPhe: 2.591 ± 0.035	9.078LeuGly: 9.078 ± 0.064	2.448LeuHis: 2.448 ± 0.038	2.992LeuIle: 2.992 ± 0.043	2.063LeuLys: 2.063 ± 0.034	11.102LeuLeu: 11.102 ± 0.088	1.588LeuMet: 1.588 ± 0.025	1.621LeuAsn: 1.621 ± 0.027	6.428LeuPro: 6.428 ± 0.066	2.098LeuGln: 2.098 ± 0.03	9.078LeuArg: 9.078 ± 0.073	5.155LeuSer: 5.155 ± 0.051	6.852LeuThr: 6.852 ± 0.057	8.689LeuVal: 8.689 ± 0.069	1.306LeuTrp: 1.306 ± 0.023	1.787LeuTyr: 1.787 ± 0.028	0.004LeuXaa: 0.004 ± 0.001
Met	2.144MetAla: 2.144 ± 0.031	0.145MetCys: 0.145 ± 0.008	0.876MetAsp: 0.876 ± 0.018	0.759MetGlu: 0.759 ± 0.017	0.434MetPhe: 0.434 ± 0.015	1.266MetGly: 1.266 ± 0.025	0.349MetHis: 0.349 ± 0.011	0.61MetIle: 0.61 ± 0.017	0.413MetLys: 0.413 ± 0.013	1.591MetLeu: 1.591 ± 0.026	0.288MetMet: 0.288 ± 0.01	0.428MetAsn: 0.428 ± 0.015	1.08MetPro: 1.08 ± 0.021	0.415MetGln: 0.415 ± 0.013	1.404MetArg: 1.404 ± 0.025	1.217MetSer: 1.217 ± 0.024	1.428MetThr: 1.428 ± 0.022	1.203MetVal: 1.203 ± 0.025	0.203MetTrp: 0.203 ± 0.01	0.324MetTyr: 0.324 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.064AsnAla: 2.064 ± 0.032	0.155AsnCys: 0.155 ± 0.009	0.941AsnAsp: 0.941 ± 0.02	0.756AsnGlu: 0.756 ± 0.019	0.454AsnPhe: 0.454 ± 0.012	1.753AsnGly: 1.753 ± 0.032	0.402AsnHis: 0.402 ± 0.013	0.557AsnIle: 0.557 ± 0.015	0.418AsnLys: 0.418 ± 0.014	1.522AsnLeu: 1.522 ± 0.029	0.268AsnMet: 0.268 ± 0.011	0.391AsnAsn: 0.391 ± 0.014	1.282AsnPro: 1.282 ± 0.024	0.454AsnGln: 0.454 ± 0.015	1.218AsnArg: 1.218 ± 0.024	0.871AsnSer: 0.871 ± 0.024	1.037AsnThr: 1.037 ± 0.021	1.307AsnVal: 1.307 ± 0.025	0.269AsnTrp: 0.269 ± 0.011	0.379AsnTyr: 0.379 ± 0.012	0.001AsnXaa: 0.001 ± 0.001
Pro	8.927ProAla: 8.927 ± 0.081	0.363ProCys: 0.363 ± 0.011	4.73ProAsp: 4.73 ± 0.053	4.19ProGlu: 4.19 ± 0.041	1.501ProPhe: 1.501 ± 0.022	7.156ProGly: 7.156 ± 0.062	1.549ProHis: 1.549 ± 0.029	1.101ProIle: 1.101 ± 0.02	1.243ProLys: 1.243 ± 0.026	5.482ProLeu: 5.482 ± 0.056	0.972ProMet: 0.972 ± 0.02	0.812ProAsn: 0.812 ± 0.022	3.72ProPro: 3.72 ± 0.059	1.803ProGln: 1.803 ± 0.038	4.241ProArg: 4.241 ± 0.039	3.024ProSer: 3.024 ± 0.04	3.118ProThr: 3.118 ± 0.043	5.431ProVal: 5.431 ± 0.056	0.912ProTrp: 0.912 ± 0.02	1.382ProTyr: 1.382 ± 0.026	0.006ProXaa: 0.006 ± 0.002
Gln	3.578GlnAla: 3.578 ± 0.048	0.182GlnCys: 0.182 ± 0.009	1.465GlnAsp: 1.465 ± 0.022	1.534GlnGlu: 1.534 ± 0.026	0.685GlnPhe: 0.685 ± 0.017	2.319GlnGly: 2.319 ± 0.04	0.705GlnHis: 0.705 ± 0.019	0.971GlnIle: 0.971 ± 0.022	0.595GlnLys: 0.595 ± 0.017	2.938GlnLeu: 2.938 ± 0.037	0.453GlnMet: 0.453 ± 0.014	0.464GlnAsn: 0.464 ± 0.015	1.614GlnPro: 1.614 ± 0.031	1.233GlnGln: 1.233 ± 0.032	2.333GlnArg: 2.333 ± 0.034	1.257GlnSer: 1.257 ± 0.023	1.227GlnThr: 1.227 ± 0.024	2.26GlnVal: 2.26 ± 0.035	0.461GlnTrp: 0.461 ± 0.013	0.598GlnTyr: 0.598 ± 0.015	0.001GlnXaa: 0.001 ± 0.001
Arg	10.903ArgAla: 10.903 ± 0.081	0.629ArgCys: 0.629 ± 0.015	4.633ArgAsp: 4.633 ± 0.044	4.733ArgGlu: 4.733 ± 0.047	2.348ArgPhe: 2.348 ± 0.032	6.361ArgGly: 6.361 ± 0.057	2.328ArgHis: 2.328 ± 0.033	2.965ArgIle: 2.965 ± 0.036	1.702ArgLys: 1.702 ± 0.03	8.876ArgLeu: 8.876 ± 0.074	1.724ArgMet: 1.724 ± 0.024	1.312ArgAsn: 1.312 ± 0.024	5.057ArgPro: 5.057 ± 0.054	2.325ArgGln: 2.325 ± 0.033	7.792ArgArg: 7.792 ± 0.072	4.084ArgSer: 4.084 ± 0.041	5.717ArgThr: 5.717 ± 0.061	6.332ArgVal: 6.332 ± 0.052	1.376ArgTrp: 1.376 ± 0.028	1.795ArgTyr: 1.795 ± 0.028	0.005ArgXaa: 0.005 ± 0.001
Ser	6.737SerAla: 6.737 ± 0.064	0.392SerCys: 0.392 ± 0.012	2.621SerAsp: 2.621 ± 0.038	2.262SerGlu: 2.262 ± 0.032	1.454SerPhe: 1.454 ± 0.024	6.134SerGly: 6.134 ± 0.06	1.038SerHis: 1.038 ± 0.019	1.234SerIle: 1.234 ± 0.027	1.056SerLys: 1.056 ± 0.025	4.672SerLeu: 4.672 ± 0.046	1.017SerMet: 1.017 ± 0.02	0.807SerAsn: 0.807 ± 0.02	3.101SerPro: 3.101 ± 0.04	1.142SerGln: 1.142 ± 0.024	3.686SerArg: 3.686 ± 0.042	2.654SerSer: 2.654 ± 0.044	2.913SerThr: 2.913 ± 0.043	4.104SerVal: 4.104 ± 0.039	0.844SerTrp: 0.844 ± 0.018	1.171SerTyr: 1.171 ± 0.022	0.004SerXaa: 0.004 ± 0.001
Thr	9.037ThrAla: 9.037 ± 0.068	0.404ThrCys: 0.404 ± 0.011	3.687ThrAsp: 3.687 ± 0.038	3.224ThrGlu: 3.224 ± 0.035	1.57ThrPhe: 1.57 ± 0.026	6.8ThrGly: 6.8 ± 0.064	1.28ThrHis: 1.28 ± 0.024	1.504ThrIle: 1.504 ± 0.027	1.191ThrLys: 1.191 ± 0.026	5.64ThrLeu: 5.64 ± 0.045	0.92ThrMet: 0.92 ± 0.02	0.903ThrAsn: 0.903 ± 0.022	4.151ThrPro: 4.151 ± 0.052	1.291ThrGln: 1.291 ± 0.024	4.064ThrArg: 4.064 ± 0.034	3.063ThrSer: 3.063 ± 0.042	3.713ThrThr: 3.713 ± 0.048	5.936ThrVal: 5.936 ± 0.055	0.894ThrTrp: 0.894 ± 0.021	1.372ThrTyr: 1.372 ± 0.029	0.002ThrXaa: 0.002 ± 0.001
Val	10.978ValAla: 10.978 ± 0.068	0.788ValCys: 0.788 ± 0.017	5.177ValAsp: 5.177 ± 0.047	4.655ValGlu: 4.655 ± 0.046	2.334ValPhe: 2.334 ± 0.034	6.713ValGly: 6.713 ± 0.065	2.082ValHis: 2.082 ± 0.031	2.549ValIle: 2.549 ± 0.034	1.648ValLys: 1.648 ± 0.028	9.427ValLeu: 9.427 ± 0.071	1.291ValMet: 1.291 ± 0.026	1.511ValAsn: 1.511 ± 0.027	5.413ValPro: 5.413 ± 0.052	2.006ValGln: 2.006 ± 0.03	7.564ValArg: 7.564 ± 0.064	4.128ValSer: 4.128 ± 0.043	5.525ValThr: 5.525 ± 0.051	8.154ValVal: 8.154 ± 0.081	1.138ValTrp: 1.138 ± 0.021	1.487ValTyr: 1.487 ± 0.028	0.004ValXaa: 0.004 ± 0.002
Trp	1.653TrpAla: 1.653 ± 0.03	0.16TrpCys: 0.16 ± 0.008	0.824TrpAsp: 0.824 ± 0.018	0.711TrpGlu: 0.711 ± 0.017	0.504TrpPhe: 0.504 ± 0.017	1.053TrpGly: 1.053 ± 0.02	0.379TrpHis: 0.379 ± 0.013	0.529TrpIle: 0.529 ± 0.014	0.349TrpLys: 0.349 ± 0.014	1.761TrpLeu: 1.761 ± 0.03	0.28TrpMet: 0.28 ± 0.012	0.389TrpAsn: 0.389 ± 0.016	0.758TrpPro: 0.758 ± 0.017	0.632TrpGln: 0.632 ± 0.017	1.397TrpArg: 1.397 ± 0.025	0.907TrpSer: 0.907 ± 0.022	0.974TrpThr: 0.974 ± 0.022	0.973TrpVal: 0.973 ± 0.019	0.331TrpTrp: 0.331 ± 0.013	0.326TrpTyr: 0.326 ± 0.011	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.777TyrAla: 2.777 ± 0.033	0.17TyrCys: 0.17 ± 0.008	1.477TyrAsp: 1.477 ± 0.031	1.35TyrGlu: 1.35 ± 0.027	0.625TyrPhe: 0.625 ± 0.016	2.243TyrGly: 2.243 ± 0.037	0.396TyrHis: 0.396 ± 0.012	0.415TyrIle: 0.415 ± 0.012	0.417TyrLys: 0.417 ± 0.013	1.958TyrLeu: 1.958 ± 0.029	0.241TyrMet: 0.241 ± 0.009	0.348TyrAsn: 0.348 ± 0.013	0.998TyrPro: 0.998 ± 0.021	0.55TyrGln: 0.55 ± 0.015	1.821TyrArg: 1.821 ± 0.028	0.889TyrSer: 0.889 ± 0.018	1.159TyrThr: 1.159 ± 0.022	1.746TyrVal: 1.746 ± 0.027	0.352TyrTrp: 0.352 ± 0.012	0.424TyrTyr: 0.424 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.008XaaAla: 0.008 ± 0.002	0.001XaaCys: 0.001 ± 0.001	0.005XaaAsp: 0.005 ± 0.001	0.002XaaGlu: 0.002 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.008XaaGly: 0.008 ± 0.002	0.002XaaHis: 0.002 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.002XaaLeu: 0.002 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.002XaaAsn: 0.002 ± 0.001	0.006XaaPro: 0.006 ± 0.002	0.001XaaGln: 0.001 ± 0.001	0.004XaaArg: 0.004 ± 0.001	0.004XaaSer: 0.004 ± 0.002	0.002XaaThr: 0.002 ± 0.001	0.002XaaVal: 0.002 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.015XaaXaa: 0.015 ± 0.004

Statistics based on 7592 proteins (2528181 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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