Torque teno indri virus 1
Average proteome isoelectric point is 7.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z1W0S6|A0A1Z1W0S6_9VIRU Capsid protein OS=Torque teno indri virus 1 OX=2010248 GN=orf1 PE=3 SV=1
MM1 pKa = 8.18 DD2 pKa = 4.52 YY3 pKa = 11.01 SRR5 pKa = 11.84 QKK7 pKa = 10.52 QNEE10 pKa = 4.38 AKK12 pKa = 9.34 WKK14 pKa = 10.32 LGINLAHH21 pKa = 6.18 STWCSCGDD29 pKa = 3.28 WFGHH33 pKa = 5.51 LTCIYY38 pKa = 8.83 YY39 pKa = 10.55 RR40 pKa = 11.84 SGEE43 pKa = 4.15 SSTAVQAKK51 pKa = 9.67 NKK53 pKa = 9.27 TPEE56 pKa = 3.91 TCLGGGGEE64 pKa = 4.32 GGVYY68 pKa = 9.81 QGGGAGGGEE77 pKa = 4.58 DD78 pKa = 3.63 GGEE81 pKa = 4.03 DD82 pKa = 3.45 ATIPDD87 pKa = 4.05 AALEE91 pKa = 4.24 DD92 pKa = 4.2 AEE94 pKa = 4.27 QQ95 pKa = 3.66
Molecular weight: 9.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.265
IPC2_protein 4.596
IPC_protein 4.444
Toseland 4.304
ProMoST 4.558
Dawson 4.393
Bjellqvist 4.546
Wikipedia 4.279
Rodwell 4.291
Grimsley 4.215
Solomon 4.393
Lehninger 4.342
Nozaki 4.52
DTASelect 4.66
Thurlkill 4.317
EMBOSS 4.291
Sillero 4.571
Patrickios 2.015
IPC_peptide 4.393
IPC2_peptide 4.558
IPC2.peptide.svr19 4.487
Protein with the highest isoelectric point:
>tr|A0A1Z1W0T3|A0A1Z1W0T3_9VIRU ORF3 OS=Torque teno indri virus 1 OX=2010248 GN=orf3 PE=4 SV=1
MM1 pKa = 7.07 PWRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 WRR10 pKa = 11.84 IPGRR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 WRR18 pKa = 11.84 WRR20 pKa = 11.84 RR21 pKa = 11.84 WWRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 YY27 pKa = 8.75 YY28 pKa = 9.2 PRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 FRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 TVRR40 pKa = 11.84 KK41 pKa = 8.16 PLNIIQYY48 pKa = 7.23 QPRR51 pKa = 11.84 IIKK54 pKa = 10.2 RR55 pKa = 11.84 CVIKK59 pKa = 10.93 GKK61 pKa = 10.14 VLVLGGHH68 pKa = 6.04 TFQTIEE74 pKa = 4.3 RR75 pKa = 11.84 FHH77 pKa = 6.08 HH78 pKa = 6.04 TKK80 pKa = 9.25 TAADD84 pKa = 3.9 GTTEE88 pKa = 4.05 TVFVHH93 pKa = 6.94 GGTTDD98 pKa = 2.59 WMTVTLQEE106 pKa = 4.94 LYY108 pKa = 10.64 KK109 pKa = 10.67 GWKK112 pKa = 8.38 QRR114 pKa = 11.84 QNTWSTSNATFDD126 pKa = 3.18 MARR129 pKa = 11.84 YY130 pKa = 9.23 KK131 pKa = 10.53 GAKK134 pKa = 6.84 FTLWKK139 pKa = 10.24 HH140 pKa = 5.53 IEE142 pKa = 3.82 HH143 pKa = 7.53 DD144 pKa = 4.39 YY145 pKa = 10.39 VVVWDD150 pKa = 4.47 TEE152 pKa = 4.14 QSALLTADD160 pKa = 3.6 IQRR163 pKa = 11.84 MQPFQAQLTEE173 pKa = 4.09 KK174 pKa = 9.7 NHH176 pKa = 5.22 IVVRR180 pKa = 11.84 SNRR183 pKa = 11.84 WGNSAPAKK191 pKa = 8.12 TLRR194 pKa = 11.84 IKK196 pKa = 10.34 PPPGMKK202 pKa = 9.8 NQWYY206 pKa = 9.15 YY207 pKa = 10.8 MSDD210 pKa = 3.1 IADD213 pKa = 3.68 KK214 pKa = 10.71 TLVNYY219 pKa = 8.88 GFSIADD225 pKa = 3.25 IRR227 pKa = 11.84 DD228 pKa = 3.26 GGAGDD233 pKa = 3.7 TGRR236 pKa = 11.84 IITPWPDD243 pKa = 2.7 GSVEE247 pKa = 4.08 NTVTEE252 pKa = 4.21 GGKK255 pKa = 10.25 KK256 pKa = 7.94 YY257 pKa = 9.34 TIQNYY262 pKa = 7.37 SWQWDD267 pKa = 3.79 LPPWQIQISDD277 pKa = 3.3 KK278 pKa = 9.99 WRR280 pKa = 11.84 QPFWMSQRR288 pKa = 11.84 GLGLTEE294 pKa = 4.33 NNWIYY299 pKa = 10.56 DD300 pKa = 3.4 INIPYY305 pKa = 8.87 YY306 pKa = 8.9 QFWWGIQGSEE316 pKa = 4.23 DD317 pKa = 3.47 PQINTDD323 pKa = 3.16 YY324 pKa = 10.32 WGKK327 pKa = 10.7 KK328 pKa = 7.65 EE329 pKa = 5.25 DD330 pKa = 3.87 GMEE333 pKa = 3.74 NLYY336 pKa = 10.03 PYY338 pKa = 10.25 KK339 pKa = 10.27 ILWWDD344 pKa = 3.28 QEE346 pKa = 4.09 ANRR349 pKa = 11.84 RR350 pKa = 11.84 LFGIKK355 pKa = 9.94 FPGVIHH361 pKa = 7.04 ILQNGPMVPHH371 pKa = 7.01 LKK373 pKa = 10.6 NPFSHH378 pKa = 7.02 IIDD381 pKa = 3.57 YY382 pKa = 10.55 KK383 pKa = 10.68 FYY385 pKa = 9.9 WEE387 pKa = 4.21 WGGHH391 pKa = 5.27 IPHH394 pKa = 6.59 SRR396 pKa = 11.84 NIEE399 pKa = 3.77 KK400 pKa = 10.54 DD401 pKa = 3.14 PRR403 pKa = 11.84 FQTFKK408 pKa = 11.05 GQDD411 pKa = 3.23 VQNPRR416 pKa = 11.84 KK417 pKa = 9.88 AYY419 pKa = 7.4 KK420 pKa = 9.96 TSLAPWDD427 pKa = 3.81 FDD429 pKa = 4.23 KK430 pKa = 11.53 DD431 pKa = 3.8 GLITKK436 pKa = 9.67 HH437 pKa = 5.74 AFRR440 pKa = 11.84 RR441 pKa = 11.84 LLSPTEE447 pKa = 4.4 DD448 pKa = 3.62 EE449 pKa = 4.87 SDD451 pKa = 3.6 CSLHH455 pKa = 6.21 FKK457 pKa = 10.4 EE458 pKa = 5.27 RR459 pKa = 11.84 VPKK462 pKa = 9.99 EE463 pKa = 3.97 QEE465 pKa = 3.86 DD466 pKa = 4.06 DD467 pKa = 3.69 SSQEE471 pKa = 3.74 EE472 pKa = 4.66 SSCNGEE478 pKa = 4.04 SDD480 pKa = 3.79 SDD482 pKa = 3.99 SDD484 pKa = 3.78 STLQIPIQKK493 pKa = 9.68 QIKK496 pKa = 9.43 LIQRR500 pKa = 11.84 RR501 pKa = 11.84 LAMDD505 pKa = 3.4 CQFRR509 pKa = 11.84 KK510 pKa = 10.01 RR511 pKa = 11.84 LRR513 pKa = 11.84 TYY515 pKa = 11.18 LKK517 pKa = 10.21 AQKK520 pKa = 10.39
Molecular weight: 62.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.164
IPC2_protein 9.311
IPC_protein 9.355
Toseland 9.999
ProMoST 9.721
Dawson 10.204
Bjellqvist 9.882
Wikipedia 10.379
Rodwell 10.57
Grimsley 10.277
Solomon 10.233
Lehninger 10.189
Nozaki 9.999
DTASelect 9.867
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.116
Patrickios 9.984
IPC_peptide 10.233
IPC2_peptide 8.492
IPC2.peptide.svr19 8.315
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
705
90
520
235.0
27.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.965 ± 1.871
1.277 ± 0.848
6.099 ± 0.526
5.106 ± 1.04
3.546 ± 0.689
7.234 ± 3.434
2.553 ± 0.199
6.099 ± 1.464
7.801 ± 2.046
5.39 ± 0.327
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.844 ± 0.222
3.83 ± 0.298
5.106 ± 0.845
6.241 ± 0.425
8.085 ± 1.979
6.95 ± 3.869
6.667 ± 0.343
2.979 ± 1.099
4.681 ± 1.056
3.546 ± 1.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here