Alteromonas phage PB15
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1J0GWA3|A0A1J0GWA3_9CAUD Gene transfer agent prohead protease OS=Alteromonas phage PB15 OX=1913113 PE=4 SV=1
MM1 pKa = 7.65 NEE3 pKa = 3.76 QTNLMRR9 pKa = 11.84 SMIAAQIHH17 pKa = 6.11 EE18 pKa = 4.91 NDD20 pKa = 3.03 GCMPYY25 pKa = 10.4 NNSGIAIMDD34 pKa = 3.34 IQFNLLWEE42 pKa = 4.24 EE43 pKa = 4.54 VYY45 pKa = 10.93 GYY47 pKa = 10.3 IPDD50 pKa = 3.89 GFEE53 pKa = 3.7 GAKK56 pKa = 10.2
Molecular weight: 6.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.933
IPC2_protein 4.215
IPC_protein 3.986
Toseland 3.834
ProMoST 4.139
Dawson 3.948
Bjellqvist 4.113
Wikipedia 3.872
Rodwell 3.834
Grimsley 3.757
Solomon 3.923
Lehninger 3.872
Nozaki 4.101
DTASelect 4.19
Thurlkill 3.884
EMBOSS 3.884
Sillero 4.101
Patrickios 0.604
IPC_peptide 3.923
IPC2_peptide 4.088
IPC2.peptide.svr19 4.007
Protein with the highest isoelectric point:
>tr|A0A1J0GWF0|A0A1J0GWF0_9CAUD Uncharacterized protein OS=Alteromonas phage PB15 OX=1913113 PE=4 SV=1
MM1 pKa = 7.16 VLVGRR6 pKa = 11.84 FLLTLVAKK14 pKa = 10.69 LLMRR18 pKa = 11.84 MTTWPWSRR26 pKa = 11.84 NVSKK30 pKa = 11.2 GEE32 pKa = 3.82 QYY34 pKa = 9.92 TRR36 pKa = 11.84 SQFFYY41 pKa = 10.84 FGDD44 pKa = 3.47
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.911
IPC_protein 10.687
Toseland 10.54
ProMoST 10.277
Dawson 10.701
Bjellqvist 10.423
Wikipedia 10.921
Rodwell 10.921
Grimsley 10.774
Solomon 10.789
Lehninger 10.76
Nozaki 10.496
DTASelect 10.423
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.613
Patrickios 10.804
IPC_peptide 10.789
IPC2_peptide 9.297
IPC2.peptide.svr19 8.366
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
61
0
61
11335
44
748
185.8
20.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.69 ± 0.619
1.306 ± 0.148
5.743 ± 0.236
6.855 ± 0.297
3.714 ± 0.2
8.152 ± 0.369
1.861 ± 0.224
6.334 ± 0.266
5.69 ± 0.386
7.569 ± 0.29
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.867 ± 0.219
4.808 ± 0.289
3.82 ± 0.253
3.926 ± 0.239
4.693 ± 0.24
7.199 ± 0.338
5.726 ± 0.381
6.149 ± 0.244
1.456 ± 0.138
3.441 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here